CropPAL

Subcellular Localization

min:

: max

Winner_takes_all: nucleus

Predictor Summary:

cytosol 4
plastid 1
mitochondrion 2

Predictors

GFP

MS/MS

Papers

Winner Takes All:	nucleus
Any Predictor:	cytosol, mitochondrion, plastid
BaCelLo:	cytosol
EpiLoc:	plastid
MultiLoc:	cytosol
Plant-mPloc:	cytosol
PProwler:	mitochondrion
WoLF PSORT:	mitochondrion
YLoc:	cytosol

nucleus: 21132161

msms PMID: 21132161 doi

B Cooper, KB Campbell, J Feng, WM Garrett, R Frederick

Soybean Genomics and Improvement Laboratory, USDA-ARS, Beltsville, MD 20705, USA. bret.cooper@ars.usda.gov

PPI

Inferred distinct locusB in Crop

locusB	locations
KRG89812
KRG94975
KRH18664
KRH37948
KRH39528
KRH45461
KRH52864
KRH62155
KRH74430
KRH75627

Inferred from Arabidopsis experimental PPI

Ath locusA	locusB	Ath locusB	Paper
AT4G37870.1	KRG89812	AT2G41090.1	17360592
AT4G37870.1	KRG94975	AT2G41090.1	17360592
AT4G37870.1	KRH18664	AT2G41090.1	17360592
AT4G37870.1	KRH37948	AT4G37870.1	21798944
AT4G37870.1	KRH39528	AT4G37870.1	21798944
AT4G37870.1	KRH45461	AT4G37870.1	21798944
AT4G37870.1	KRH52864	AT4G37870.1	21798944
AT4G37870.1	KRH62155	AT4G37870.1	21798944
AT4G37870.1	KRH74430	AT4G37870.1	21798944
AT4G37870.1	KRH75627	AT4G37870.1	21798944
AT4G37870.1	KRG89812	AT5G21274.1	17360592
AT4G37870.1	KRG94975	AT5G21274.1	17360592
AT4G37870.1	KRH18664	AT5G21274.1	17360592

Homology

Paralog

locus	Identity	Homology Identity

Ortholog

locus	Homology Species	Location	Identity	Homology Identity
KRH74430	Soybean	endoplasmic reticulum	96.22	96.36
VIT_00s1995g00010.t01	Wine grape	cytosol	60.97	90.36
KRH52864	Soybean	cytosol	48.26	87.16
KRH75627	Soybean	cytosol	83.96	85.65
CDY31619	Canola	cytosol	69.44	85.63
KRH45461	Soybean	nucleus	80.94	84.92
KXG39751	Sorghum	cytosol	82.6	82.85
CDX75681	Canola	cytosol	82.75	81.76
Bra011792.1-P	Field mustard	cytosol	82.6	81.61
CDX72658	Canola	cytosol	78.37	81.19
CDX69339	Canola	cytosol	78.82	81.15
AT4G37870.1	Thale cress	cytosol	82.3	81.07
Bra017856.1-P	Field mustard	cytosol	81.54	81.05
TraesCS4D01G220500.1	Wheat	cytosol	81.24	80.39
KRH62155	Soybean	cytosol, nucleus	81.39	80.06
TraesCS4B01G220200.1	Wheat	cytosol	80.64	79.55
KRH37948	Soybean	cytosol	14.52	75.59
TraesCS4A01G083900.1	Wheat	mitochondrion	81.54	74.65
HORVU4Hr1G062440.2	Barley	cytosol	73.37	72.07
Os10t0204300-01	Rice	cytosol	3.78	52.08
Zm00001d008318_P001	Maize	cytosol	16.79	49.55
GSMUA_Achr4P20850_001	Banana	cytosol	8.62	47.9

Protein Annotations

KEGG:00010+4.1.1.49	KEGG:00020+4.1.1.49	KEGG:00620+4.1.1.49	KEGG:00710+4.1.1.49	EntrezGene:100786257	Gene3D:2.170.8.10
MapMan:3.10.1	Gene3D:3.40.449.10	Gene3D:3.90.228.20	EMBL:ACUP02005942	ncoils:Coil	EnsemblPlantsGene:GLYMA_09G203500
GO:GO:0000166	GO:GO:0003674	GO:GO:0003824	GO:GO:0004611	GO:GO:0004612	GO:GO:0005488
GO:GO:0005524	GO:GO:0005575	GO:GO:0005622	GO:GO:0005623	GO:GO:0005737	GO:GO:0005829
GO:GO:0005975	GO:GO:0006094	GO:GO:0008150	GO:GO:0008152	GO:GO:0009058	GO:GO:0017076
UniProt:I1L4W3	InterPro:IPR008210	InterPro:IPR013035	EnsemblPlants:KRH39528	ProteinID:KRH39528	ProteinID:KRH39528.1
HAMAP:MF_00453	InterPro:PEPCK_ATP_CS	InterPro:PEP_carboxykinase_ATP	InterPro:PEP_carboxykinase_C	InterPro:PEP_carboxykinase_N	PFAM:PF01293
ScanProsite:PS00532	PANTHER:PTHR30031	PANTHER:PTHR30031:SF4	MetaCyc:PWY-561	MetaCyc:PWY-7117	SUPFAM:SSF53795
SUPFAM:SSF68923	TIGRFAMs:TIGR00224	UniParc:UPI0002337BB3	SEG:seg	:	:

Description

hypothetical protein

Coordinates

chr9:+:42786762..42795194

Molecular Weight (calculated)

73192.9 Da

IEP (calculated)

7.533

GRAVY (calculated)

-0.409

Length

661 amino acids

Sequence
(BLAST)

001: MASNGNGSVA NGNGTARNGL AKIQTHKKHN GICHDDSGPT VKAQTIDELH SLQKKKSAPT TPVTGFQAPF ATLTEEERHK QQLQSISASL ASLTRETGPN
101: LVKGDPARKS DTQKVTHVHH HHVSTPTIAV SDSALKFTHV LYNLSPAELY EQAIKYEKGS FISSTGALAT LSGAKTGRSP RDKRVVKDDL TKNDLWWGKG
201: SPNIEMDEHS FMVNRERAVD YLNSLDKVFV NDQFLNWDTE NRIKVRIVSA RAYHSLFMHN MCIRPTPEEL ENFGTPDFTI YNAGQFPCNR YTHYMTSSTS
301: IDLNLARREM VILGTQYAGE MKKGLFSVMH YLMPKRQILS LHSGCNMGKD GDVALFFGLS GTGKTTLSTD HNRYLIGDDE HCWGDNGVSN IEGGCYAKCI
401: DLSREKEPDI WNAIRFGTVL ENVVFDEHDR EVDYSDKSVT ENTRAAYPIE YIPNAKLPCV GPHPKNVILL ACDAFGVLPP VSKLNLAQTM YHFISGYTAL
501: VAGTEEGIKE PQATFSACFG AAFIMLHPTK YAAMLAEKMQ KHGATGWLVN TGWSAGSYGN GSRIKLQYTR KIIDAIHSGS LLNAEYKKTE IFGLEIPTEV
601: EGVPSEILDP MNTWSNKKAY KETLLKLAGL FKNNFETFTN YKIGKGNNNL TEEILAAGPI F

Best Arabidopsis Sequence Match ( AT4G37870.1 )
(BLAST)

001: MSAGNGNATN GDGGFSFPKG PVMPKITTGA AKRGSGVCHD DSGPTVNATT IDELHSLQKK RSAPTTPINQ NAAAAFAAVS EEERQKIQLQ SISASLASLT
101: RESGPKVVRG DPAEKKTDGS TTPAYAHGQH HSIFSPATGA VSDSSLKFTH VLYNLSPAEL YEQAIKYEKG SFITSNGALA TLSGAKTGRA PRDKRVVRDA
201: TTEDELWWGK GSPNIEMDEH TFMVNRERAV DYLNSLEKVF VNDQYLNWDP ENRIKVRIVS ARAYHSLFMH NMCIRPTQEE LESFGTPDFT IYNAGQFPCN
301: RYTHYMTSST SVDLNLARRE MVILGTQYAG EMKKGLFSVM HYLMPKRRIL SLHSGCNMGK DGDVALFFGL SGTGKTTLST DHNRYLIGDD EHCWTETGVS
401: NIEGGCYAKC VDLSREKEPD IWNAIKFGTV LENVVFDEHT REVDYSDKSV TENTRAAYPI EFIPNAKIPC VGPHPTNVIL LACDAFGVLP PVSKLNLAQT
501: MYHFISGYTA LVAGTEDGIK EPTATFSACF GAAFIMLHPT KYAAMLAEKM KSQGATGWLV NTGWSGGSYG VGNRIKLAYT RKIIDAIHSG SLLKANYKKT
601: EIFGFEIPTE IEGIPSEILD PVNSWSDKKA HKDTLVKLGG LFKKNFEVFA NHKIGVDGKL TEEILAAGPI F

Arabidopsis Description

PCKAPhosphoenolpyruvate carboxykinase (ATP)-like protein [Source:UniProtKB/TrEMBL;Acc:Q0WWL8]
SUBAcon: [cytosol]

Hydropathy Plot

About CropPAL

The Protein Annotated Locations Database (CropPAL) houses large scale proteomic and GFP localization data from published experimental studies in Soybean (Glycine max), Maize (Zea mays), Wheat (Triticum aestivum), Barley (Hordeum vulgare), Rice (Oryza sativa), Field mustard (Brassica rapa), Canola (Brassica napus), Sorghum (Sorghum bicolor), Potato (Solanum tuberosum), Tomato (Solanum lycopersicum), Banana (Musa acuminata) and Wine grape (Vitis vinifera) as well as precomputed predictions for protein subcellular localizations using protein sequences.