CropPAL

Subcellular Localization

min:

: max

Winner_takes_all: nucleus

Predictor Summary:

cytosol 3
mitochondrion 1
nucleus 1

Predictors

GFP

MS/MS

Papers

Winner Takes All:	nucleus
Any Predictor:	cytosol, mitochondrion, nucleus
BaCelLo:	cytosol
MultiLoc:	cytosol
PProwler:	mitochondrion
WoLF PSORT:	nucleus
YLoc:	cytosol

nucleus: 21132161

msms PMID: 21132161 doi

B Cooper, KB Campbell, J Feng, WM Garrett, R Frederick

Soybean Genomics and Improvement Laboratory, USDA-ARS, Beltsville, MD 20705, USA. bret.cooper@ars.usda.gov

PPI

Inferred distinct locusB in Crop

locusB	locations
KRG89812
KRG94975
KRH18664
KRH37948
KRH39528
KRH45461
KRH52864
KRH62155
KRH74430
KRH75627

Inferred from Arabidopsis experimental PPI

Ath locusA	locusB	Ath locusB	Paper
AT4G37870.1	KRG89812	AT2G41090.1	17360592
AT4G37870.1	KRG94975	AT2G41090.1	17360592
AT4G37870.1	KRH18664	AT2G41090.1	17360592
AT4G37870.1	KRH37948	AT4G37870.1	21798944
AT4G37870.1	KRH39528	AT4G37870.1	21798944
AT4G37870.1	KRH45461	AT4G37870.1	21798944
AT4G37870.1	KRH52864	AT4G37870.1	21798944
AT4G37870.1	KRH62155	AT4G37870.1	21798944
AT4G37870.1	KRH74430	AT4G37870.1	21798944
AT4G37870.1	KRH75627	AT4G37870.1	21798944
AT4G37870.1	KRG89812	AT5G21274.1	17360592
AT4G37870.1	KRG94975	AT5G21274.1	17360592
AT4G37870.1	KRH18664	AT5G21274.1	17360592

Homology

Paralog

locus	Identity	Homology Identity

Ortholog

locus	Homology Species	Location	Identity	Homology Identity
KRH75627	Soybean	cytosol	94.92	92.28
KRH37948	Soybean	cytosol	18.1	89.76
VIT_00s1995g00010.t01	Wine grape	cytosol	60.48	85.43
KRH52864	Soybean	cytosol	49.37	84.97
KRH74430	Soybean	endoplasmic reticulum	85.08	81.21
KRH39528	Soybean	nucleus	84.92	80.94
CDY31619	Canola	cytosol	68.57	80.6
KXG39751	Sorghum	cytosol	80.0	76.48
CDX75681	Canola	cytosol	81.11	76.38
Bra011792.1-P	Field mustard	cytosol	80.95	76.23
AT4G37870.1	Thale cress	cytosol	80.63	75.71
CDX69339	Canola	cytosol	77.14	75.7
Bra017856.1-P	Field mustard	cytosol	79.84	75.64
CDX72658	Canola	cytosol	76.03	75.08
KRH62155	Soybean	cytosol, nucleus	79.84	74.85
TraesCS4D01G220500.1	Wheat	cytosol	79.37	74.85
TraesCS4B01G220200.1	Wheat	cytosol	78.73	74.03
TraesCS4A01G083900.1	Wheat	mitochondrion	79.52	69.39
HORVU4Hr1G062440.2	Barley	cytosol	71.9	67.31
Zm00001d008318_P001	Maize	cytosol	17.94	50.45
Os10t0204300-01	Rice	cytosol	3.33	43.75
GSMUA_Achr4P20850_001	Banana	cytosol	6.67	35.29

Protein Annotations

KEGG:00010+4.1.1.49	KEGG:00020+4.1.1.49	KEGG:00620+4.1.1.49	KEGG:00710+4.1.1.49	Gene3D:2.170.8.10	MapMan:3.10.1
Gene3D:3.40.449.10	Gene3D:3.90.228.20	UniProt:A0A0R0IZ72	EMBL:ACUP02005227	EMBL:ACUP02005228	EnsemblPlantsGene:GLYMA_08G273100
GO:GO:0000166	GO:GO:0003674	GO:GO:0003824	GO:GO:0004611	GO:GO:0004612	GO:GO:0005488
GO:GO:0005524	GO:GO:0005575	GO:GO:0005622	GO:GO:0005623	GO:GO:0005737	GO:GO:0005829
GO:GO:0005975	GO:GO:0006094	GO:GO:0008150	GO:GO:0008152	GO:GO:0009058	GO:GO:0017076
InterPro:IPR008210	InterPro:IPR013035	EnsemblPlants:KRH45461	ProteinID:KRH45461	ProteinID:KRH45461.1	HAMAP:MF_00453
InterPro:PEPCK_ATP_CS	InterPro:PEP_carboxykinase_ATP	InterPro:PEP_carboxykinase_C	InterPro:PEP_carboxykinase_N	PFAM:PF01293	PIRSF:PIRSF006294
ScanProsite:PS00532	PANTHER:PTHR30031	PANTHER:PTHR30031:SF4	MetaCyc:PWY-561	MetaCyc:PWY-7117	SUPFAM:SSF53795
SUPFAM:SSF68923	TIGRFAMs:TIGR00224	UniParc:UPI0006ED6F0A	SEG:seg	:	:

Description

hypothetical protein

Coordinates

chr8:-:35775129..35783047

Molecular Weight (calculated)

69675.6 Da

IEP (calculated)

6.211

GRAVY (calculated)

-0.355

Length

630 amino acids

Sequence
(BLAST)

001: MPTSDYDGNG NGLTKIEVGD DEEGHPTVKA QTIDELHSLQ KKKSAPSTPK GTFPASNSVF TEERNKQQLE SISASLASLT RESGPKVVKG DPAARKFEGS
101: RVEHVPHQIL TPTIAVSDSA LKFTHVLYNL SPAELYEQAI KYEKGSFITS TGALATLSGA KTGRCPRDKR VVKDDMDEHS FMVNRERAVD YLNSLDKVFV
201: NDQFLNWDPE NKIKVRIVSA RAYHSLFMHN MCIRPTPEEL ENFGTPDFTI YNAGQFPCNR YTHYMTSSTS IDLSLARKEM VILGTQYAGE MKKGLFSLMH
301: YLMPKRQILS LHSGCNMGKG GDVALFFGLS GTGKTTLSTD HNRYLIGDDE HCWSESGVSN IEGGCYAKCI DLSRENEPDI WNAIKFGTVL ENVVFDEHFR
401: EVDYTDKSVT ENTRAAYPIE YIPNVKLPCV GPHPKNVILL ACDAFGVLPP VSRLNLSQTM YHFISGYTAL VAGTEEGIKE PKATFSACFG AAFIMLHPTK
501: YAAMLAEKMQ NHGANGWLVN TGWSGGSYGC GNRIKLSYTR KIIDAIHSGS LLDAEYKKTE IFGLEIPNKV EGVPSEILEP ENTWSDKQAY KDTLLKLAGL
601: FNKNFETFTI GENNQVTEEI LAAGPIIGDA

Best Arabidopsis Sequence Match ( AT4G37870.1 )
(BLAST)

001: MSAGNGNATN GDGGFSFPKG PVMPKITTGA AKRGSGVCHD DSGPTVNATT IDELHSLQKK RSAPTTPINQ NAAAAFAAVS EEERQKIQLQ SISASLASLT
101: RESGPKVVRG DPAEKKTDGS TTPAYAHGQH HSIFSPATGA VSDSSLKFTH VLYNLSPAEL YEQAIKYEKG SFITSNGALA TLSGAKTGRA PRDKRVVRDA
201: TTEDELWWGK GSPNIEMDEH TFMVNRERAV DYLNSLEKVF VNDQYLNWDP ENRIKVRIVS ARAYHSLFMH NMCIRPTQEE LESFGTPDFT IYNAGQFPCN
301: RYTHYMTSST SVDLNLARRE MVILGTQYAG EMKKGLFSVM HYLMPKRRIL SLHSGCNMGK DGDVALFFGL SGTGKTTLST DHNRYLIGDD EHCWTETGVS
401: NIEGGCYAKC VDLSREKEPD IWNAIKFGTV LENVVFDEHT REVDYSDKSV TENTRAAYPI EFIPNAKIPC VGPHPTNVIL LACDAFGVLP PVSKLNLAQT
501: MYHFISGYTA LVAGTEDGIK EPTATFSACF GAAFIMLHPT KYAAMLAEKM KSQGATGWLV NTGWSGGSYG VGNRIKLAYT RKIIDAIHSG SLLKANYKKT
601: EIFGFEIPTE IEGIPSEILD PVNSWSDKKA HKDTLVKLGG LFKKNFEVFA NHKIGVDGKL TEEILAAGPI F

Arabidopsis Description

PCKAPhosphoenolpyruvate carboxykinase (ATP)-like protein [Source:UniProtKB/TrEMBL;Acc:Q0WWL8]
SUBAcon: [cytosol]

Hydropathy Plot

About CropPAL

The Protein Annotated Locations Database (CropPAL) houses large scale proteomic and GFP localization data from published experimental studies in Soybean (Glycine max), Maize (Zea mays), Wheat (Triticum aestivum), Barley (Hordeum vulgare), Rice (Oryza sativa), Field mustard (Brassica rapa), Canola (Brassica napus), Sorghum (Sorghum bicolor), Potato (Solanum tuberosum), Tomato (Solanum lycopersicum), Banana (Musa acuminata) and Wine grape (Vitis vinifera) as well as precomputed predictions for protein subcellular localizations using protein sequences.