CropPAL

Subcellular Localization

min:

: max

Winner_takes_all: nucleus, cytosol

Predictor Summary:

cytosol 5
plastid 1
mitochondrion 1

Predictors

GFP

MS/MS

Papers

nucleus: 21132161

msms PMID: 21132161 doi

B Cooper, KB Campbell, J Feng, WM Garrett, R Frederick

Soybean Genomics and Improvement Laboratory, USDA-ARS, Beltsville, MD 20705, USA. bret.cooper@ars.usda.gov

PPI

Inferred distinct locusB in Crop

locusB	locations
KRG89812
KRG94975
KRH18664
KRH37948
KRH39528
KRH45461
KRH52864
KRH62155
KRH74430
KRH75627

Inferred from Arabidopsis experimental PPI

Ath locusA	locusB	Ath locusB	Paper
AT4G37870.1	KRG89812	AT2G41090.1	17360592
AT4G37870.1	KRG94975	AT2G41090.1	17360592
AT4G37870.1	KRH18664	AT2G41090.1	17360592
AT4G37870.1	KRH37948	AT4G37870.1	21798944
AT4G37870.1	KRH39528	AT4G37870.1	21798944
AT4G37870.1	KRH45461	AT4G37870.1	21798944
AT4G37870.1	KRH52864	AT4G37870.1	21798944
AT4G37870.1	KRH62155	AT4G37870.1	21798944
AT4G37870.1	KRH74430	AT4G37870.1	21798944
AT4G37870.1	KRH75627	AT4G37870.1	21798944
AT4G37870.1	KRG89812	AT5G21274.1	17360592
AT4G37870.1	KRG94975	AT5G21274.1	17360592
AT4G37870.1	KRH18664	AT5G21274.1	17360592

Homology

Paralog

locus	Identity	Homology Identity

Ortholog

locus	Homology Species	Location	Identity	Homology Identity
KRH52864	Soybean	cytosol	52.53	96.45
CDY31619	Canola	cytosol	66.52	83.4
KRH74430	Soybean	endoplasmic reticulum	80.51	81.97
KRH39528	Soybean	nucleus	80.06	81.39
KXG39751	Sorghum	cytosol	78.57	80.12
KRH45461	Soybean	nucleus	74.85	79.84
KRH75627	Soybean	cytosol	76.49	79.32
AT4G37870.1	Thale cress	cytosol	78.72	78.84
CDX75681	Canola	cytosol	78.42	78.77
Bra017856.1-P	Field mustard	cytosol	77.68	78.5
Bra011792.1-P	Field mustard	cytosol	78.12	78.48
TraesCS4D01G220500.1	Wheat	cytosol	77.98	78.44
Solyc04g076880.2.1	Tomato	cytosol, extracellular, nucleus, plastid	77.23	78.4
CDX72658	Canola	cytosol	74.4	78.37
PGSC0003DMT400016354	Potato	cytosol	77.08	78.25
CDX69339	Canola	cytosol	74.26	77.73
TraesCS4B01G220200.1	Wheat	cytosol	77.23	77.46
Solyc12g088160.1.1	Tomato	cytosol, plastid	73.36	75.73
PGSC0003DMT400041521	Potato	cytosol	73.51	75.42
PGSC0003DMT400005163	Potato	cytosol	73.36	75.27
TraesCS4A01G083900.1	Wheat	mitochondrion	78.27	72.85
HORVU4Hr1G062440.2	Barley	cytosol	69.64	69.54
KRH37948	Soybean	cytosol	13.1	69.29
PGSC0003DMT400005170	Potato	cytosol	34.08	66.38
Zm00001d008318_P001	Maize	cytosol	16.22	48.66
Os10t0204300-01	Rice	cytosol	3.42	47.92
GSMUA_Achr4P20850_001	Banana	cytosol	7.44	42.02

Protein Annotations

KEGG:00010+4.1.1.49	KEGG:00020+4.1.1.49	KEGG:00620+4.1.1.49	KEGG:00710+4.1.1.49	EntrezGene:100781710	Gene3D:2.170.8.10
MapMan:3.10.1	Gene3D:3.40.449.10	Gene3D:3.90.228.20	EMBL:ACUP02002225	ncoils:Coil	EnsemblPlantsGene:GLYMA_04G089700
GO:GO:0000166	GO:GO:0003674	GO:GO:0003824	GO:GO:0004611	GO:GO:0004612	GO:GO:0005488
GO:GO:0005524	GO:GO:0005575	GO:GO:0005622	GO:GO:0005623	GO:GO:0005737	GO:GO:0005829
GO:GO:0005975	GO:GO:0006094	GO:GO:0008150	GO:GO:0008152	GO:GO:0009058	GO:GO:0017076
UniProt:I1JV14	InterPro:IPR008210	InterPro:IPR013035	EnsemblPlants:KRH62155	ProteinID:KRH62155	ProteinID:KRH62155.1
HAMAP:MF_00453	InterPro:PEPCK_ATP_CS	InterPro:PEP_carboxykinase_ATP	InterPro:PEP_carboxykinase_C	InterPro:PEP_carboxykinase_N	PFAM:PF01293
ScanProsite:PS00532	PANTHER:PTHR30031	PANTHER:PTHR30031:SF4	MetaCyc:PWY-561	MetaCyc:PWY-7117	SUPFAM:SSF53795
SUPFAM:SSF68923	TIGRFAMs:TIGR00224	UniParc:UPI0002339660	SEG:seg	:	:

Description

hypothetical protein

Coordinates

chr4:-:7821056..7827386

Molecular Weight (calculated)

74458.2 Da

IEP (calculated)

6.842

GRAVY (calculated)

-0.392

Length

672 amino acids

Sequence
(BLAST)

001: METITENGSD GVSLARNTSN NARRGLPMIQ TNMKPTDSEF QVCHDDSTPP VRAQTIHELH SLQKKKSTPS TPLSATQGPF ATVSDEERQK QQLQSISASL
101: ASLTRESGPK VVKGDPATKA EKGTHVHHHP QPHYFASTAF DVSDSALKFT HVLYNLSPAE LYEQAIKHEK GSFITATGAL ATLSGAKTGR SPRDKRVVRD
201: DTTEHDLWWG KGSPNIEMDE HTFLINRERA VDYLNSLEKV YVNDQFLNWD PEHRIKVRIV SARAYHSLFM HNMCIRPTPE ELEEFGTPDF TIYNAGQFPC
301: NRYTHYMTSS TSIDINIARK EMVILGTQYA GEMKKGLFGL MHYLMPKRNI LSLHSGCNMG KDGDVALFFG LSGTGKTTLS TDHNRYLIGD DEHCWSENGV
401: ANIEGGCYAK CVDLSKDKEP DIWNAIKFGT VLENVVFDEH TREVDFSDKS VTENTRAAYP IEYIPNAKIP CVAPHAKNVI LLACDAFGVL PPVSKLSLAQ
501: TMYHFISGYT ALVAGTEDGI KEPQATFSAC FGAAFIMMHP TKYAAMLAEK MQKHGATGWL VNTGWSGGSY GLGNRIKLAY TRKIIDAIHS GSLLNAQYTK
601: TEVFGLEIPT ALEGVPSEIL DPVNTWSDKK AYQDTRLKLA SLFKKNFDGF TTYKIGGDQK LTEEIVSAGP IF

Best Arabidopsis Sequence Match ( AT4G37870.1 )
(BLAST)

001: MSAGNGNATN GDGGFSFPKG PVMPKITTGA AKRGSGVCHD DSGPTVNATT IDELHSLQKK RSAPTTPINQ NAAAAFAAVS EEERQKIQLQ SISASLASLT
101: RESGPKVVRG DPAEKKTDGS TTPAYAHGQH HSIFSPATGA VSDSSLKFTH VLYNLSPAEL YEQAIKYEKG SFITSNGALA TLSGAKTGRA PRDKRVVRDA
201: TTEDELWWGK GSPNIEMDEH TFMVNRERAV DYLNSLEKVF VNDQYLNWDP ENRIKVRIVS ARAYHSLFMH NMCIRPTQEE LESFGTPDFT IYNAGQFPCN
301: RYTHYMTSST SVDLNLARRE MVILGTQYAG EMKKGLFSVM HYLMPKRRIL SLHSGCNMGK DGDVALFFGL SGTGKTTLST DHNRYLIGDD EHCWTETGVS
401: NIEGGCYAKC VDLSREKEPD IWNAIKFGTV LENVVFDEHT REVDYSDKSV TENTRAAYPI EFIPNAKIPC VGPHPTNVIL LACDAFGVLP PVSKLNLAQT
501: MYHFISGYTA LVAGTEDGIK EPTATFSACF GAAFIMLHPT KYAAMLAEKM KSQGATGWLV NTGWSGGSYG VGNRIKLAYT RKIIDAIHSG SLLKANYKKT
601: EIFGFEIPTE IEGIPSEILD PVNSWSDKKA HKDTLVKLGG LFKKNFEVFA NHKIGVDGKL TEEILAAGPI F

Arabidopsis Description

PCKAPhosphoenolpyruvate carboxykinase (ATP)-like protein [Source:UniProtKB/TrEMBL;Acc:Q0WWL8]
SUBAcon: [cytosol]

Hydropathy Plot

About CropPAL

The Protein Annotated Locations Database (CropPAL) houses large scale proteomic and GFP localization data from published experimental studies in Soybean (Glycine max), Maize (Zea mays), Wheat (Triticum aestivum), Barley (Hordeum vulgare), Rice (Oryza sativa), Field mustard (Brassica rapa), Canola (Brassica napus), Sorghum (Sorghum bicolor), Potato (Solanum tuberosum), Tomato (Solanum lycopersicum), Banana (Musa acuminata) and Wine grape (Vitis vinifera) as well as precomputed predictions for protein subcellular localizations using protein sequences.