CropPAL

Subcellular Localization

min:

: max

Winner_takes_all: endoplasmic reticulum

Predictor Summary:

cytosol 4
plastid 1
mitochondrion 2

Predictors

GFP

MS/MS

Papers

nucleus: 21132161
endoplasmic reticulum: 27224218
endoplasmic reticulum: 28499913

msms PMID: 21132161 doi

B Cooper, KB Campbell, J Feng, WM Garrett, R Frederick

Soybean Genomics and Improvement Laboratory, USDA-ARS, Beltsville, MD 20705, USA. bret.cooper@ars.usda.gov

msms PMID: 28499913 doi

S Komatsu, X Wang, X Yin, Y Nanjo, H Ohyanagi, K Sakata

Department of Life Science and Informatics, Maebashi Institute of Technology, Maebashi 371-0816, Japan. Electronic address: ksakata@maebashi-it.ac.jp., Institute of Crop Science, National Agriculture and Food Research Organization, Tsukuba 305-8518, Japan., Institute of Crop Science, National Agriculture and Food Research Organization, Tsukuba 305-8518, Japan; Faculty of Life and Environmental Sciences, University of Tsukuba, Tsukuba 305-8572, Japan., Institute of Crop Science, National Agriculture and Food Research Organization, Tsukuba 305-8518, Japan; Faculty of Life and Environmental Sciences, University of Tsukuba, Tsukuba 305-8572, Japan. Electronic address: komatsu.setsuko.fu@u.tsukuba.ac.jp., King Abdullah University of Science and Technology (KAUST), Computational Bioscience Research Center (CBRC), Thuwal 23955-6900, Saudi Arabia.

msms PMID: 27224218 doi

X Wang, S Komatsu

Graduate School of Life and Environmental Sciences, University of Tsukuba , Tsukuba 305-8572, Japan., National Institute of Crop Science, National Agriculture and Food Research Organization , Tsukuba 305-8518, Japan.

PPI

Inferred distinct locusB in Crop

locusB	locations
KRG89812
KRG94975
KRH18664
KRH37948
KRH39528
KRH45461
KRH52864
KRH62155
KRH74430
KRH75627

Inferred from Arabidopsis experimental PPI

Ath locusA	locusB	Ath locusB	Paper
AT4G37870.1	KRG89812	AT2G41090.1	17360592
AT4G37870.1	KRG94975	AT2G41090.1	17360592
AT4G37870.1	KRH18664	AT2G41090.1	17360592
AT4G37870.1	KRH37948	AT4G37870.1	21798944
AT4G37870.1	KRH39528	AT4G37870.1	21798944
AT4G37870.1	KRH45461	AT4G37870.1	21798944
AT4G37870.1	KRH52864	AT4G37870.1	21798944
AT4G37870.1	KRH62155	AT4G37870.1	21798944
AT4G37870.1	KRH74430	AT4G37870.1	21798944
AT4G37870.1	KRH75627	AT4G37870.1	21798944
AT4G37870.1	KRG89812	AT5G21274.1	17360592
AT4G37870.1	KRG94975	AT5G21274.1	17360592
AT4G37870.1	KRH18664	AT5G21274.1	17360592

Homology

Paralog

locus	Identity	Homology Identity

Ortholog

locus	Homology Species	Location	Identity	Homology Identity
KRH39528	Soybean	nucleus	96.36	96.22
VIT_00s1995g00010.t01	Wine grape	cytosol	60.91	90.13
KRH52864	Soybean	cytosol	48.48	87.43
KRH75627	Soybean	cytosol	84.55	86.11
CDY31619	Canola	cytosol	69.85	86.01
KRH45461	Soybean	nucleus	81.21	85.08
KXG39751	Sorghum	cytosol	83.18	83.31
CDX75681	Canola	cytosol	82.88	81.76
Bra011792.1-P	Field mustard	cytosol	82.73	81.61
AT4G37870.1	Thale cress	cytosol	82.58	81.22
CDX72658	Canola	cytosol	78.48	81.19
CDX69339	Canola	cytosol	78.94	81.15
Bra017856.1-P	Field mustard	cytosol	81.67	81.05
TraesCS4D01G220500.1	Wheat	cytosol	81.82	80.84
KRH62155	Soybean	cytosol, nucleus	81.97	80.51
TraesCS4B01G220200.1	Wheat	cytosol	81.21	80.0
TraesCS4A01G083900.1	Wheat	mitochondrion	82.12	75.07
KRH37948	Soybean	cytosol	14.39	74.8
HORVU4Hr1G062440.2	Barley	cytosol	73.48	72.07
Os10t0204300-01	Rice	cytosol	3.79	52.08
Zm00001d008318_P001	Maize	cytosol	16.97	50.0
GSMUA_Achr4P20850_001	Banana	cytosol	8.48	47.06

Protein Annotations

KEGG:00010+4.1.1.49	KEGG:00020+4.1.1.49	KEGG:00620+4.1.1.49	KEGG:00710+4.1.1.49	EntrezGene:100791015	Gene3D:2.170.8.10
MapMan:3.10.1	Gene3D:3.40.449.10	Gene3D:3.90.228.20	EMBL:ACUP02000025	ncoils:Coil	EnsemblPlantsGene:GLYMA_01G019200
GO:GO:0000166	GO:GO:0003674	GO:GO:0003824	GO:GO:0004611	GO:GO:0004612	GO:GO:0005488
GO:GO:0005524	GO:GO:0005575	GO:GO:0005622	GO:GO:0005623	GO:GO:0005737	GO:GO:0005829
GO:GO:0005975	GO:GO:0006094	GO:GO:0008150	GO:GO:0008152	GO:GO:0009058	GO:GO:0017076
UniProt:I1J4W3	InterPro:IPR008210	InterPro:IPR013035	EnsemblPlants:KRH74430	ProteinID:KRH74430	ProteinID:KRH74430.1
HAMAP:MF_00453	InterPro:PEPCK_ATP_CS	InterPro:PEP_carboxykinase_ATP	InterPro:PEP_carboxykinase_C	InterPro:PEP_carboxykinase_N	PFAM:PF01293
ScanProsite:PS00532	PANTHER:PTHR30031	PANTHER:PTHR30031:SF4	MetaCyc:PWY-561	MetaCyc:PWY-7117	SUPFAM:SSF53795
SUPFAM:SSF68923	TIGRFAMs:TIGR00224	UniParc:UPI00023379FE	SEG:seg	:	:

Description

hypothetical protein

Coordinates

chr1:-:1839153..1846859

Molecular Weight (calculated)

73107.8 Da

IEP (calculated)

7.237

GRAVY (calculated)

-0.401

Length

660 amino acids

Sequence
(BLAST)

001: MASNGNGSVA NGNGSARNGL AKIQTQKKHD GICHDDSGPT VKAQTIDELH SLQKKKSAPT TPVTGFQAPF ATLTEEERHK QQLQSISASL ASLTRETGPK
101: LVKGDPARKS DTQKVTHVHH HVSTPTIVVS DSSLKFTHVL YNLSPAELYE QAIKYEKGSF ITSTGALATL SGAKTGRSPR DKRVVKDDLT ENELWWGKGS
201: PNIEMDEHTF MVNRERAVDY LNSLDKVFVN DQFLNWDTEN RIKVRIVSAR AYHSLFMHNM CIRPTPEELE NFGTPDFTIY NAGQFPCNRY THYMTSSTSI
301: DLNLARREMV ILGTQYAGEM KKGLFGVMHY LMPKRQILSL HSGCNMGKDG DVALFFGLSG TGKTTLSTDH NRYLIGDDEH CWGDNGVSNI EGGCYAKCID
401: LSREKEPDIW NAIRFGTVLE NVVFDEHDRE VDYSDKSVTE NTRAAYPIEY IPNAKLPCVG PHPKNVILLA CDAFGVLPPV SKLNLAQTMY HFISGYTALV
501: AGTEEGIKEP QATFSACFGA AFIMLHPTKY AAMLAEKMQK HGATGWLVNT GWSAGSYGYG SRIKLQYTRK IIDAIHSGSL LNAEYKKTEI FGLEIPTEVE
601: GVPSEILDPV NTWSNKKGYK ETLLKLAGLF KNNFETFTNY KIGKGNNNLT EEILAAGPIF

Best Arabidopsis Sequence Match ( AT4G37870.1 )
(BLAST)

001: MSAGNGNATN GDGGFSFPKG PVMPKITTGA AKRGSGVCHD DSGPTVNATT IDELHSLQKK RSAPTTPINQ NAAAAFAAVS EEERQKIQLQ SISASLASLT
101: RESGPKVVRG DPAEKKTDGS TTPAYAHGQH HSIFSPATGA VSDSSLKFTH VLYNLSPAEL YEQAIKYEKG SFITSNGALA TLSGAKTGRA PRDKRVVRDA
201: TTEDELWWGK GSPNIEMDEH TFMVNRERAV DYLNSLEKVF VNDQYLNWDP ENRIKVRIVS ARAYHSLFMH NMCIRPTQEE LESFGTPDFT IYNAGQFPCN
301: RYTHYMTSST SVDLNLARRE MVILGTQYAG EMKKGLFSVM HYLMPKRRIL SLHSGCNMGK DGDVALFFGL SGTGKTTLST DHNRYLIGDD EHCWTETGVS
401: NIEGGCYAKC VDLSREKEPD IWNAIKFGTV LENVVFDEHT REVDYSDKSV TENTRAAYPI EFIPNAKIPC VGPHPTNVIL LACDAFGVLP PVSKLNLAQT
501: MYHFISGYTA LVAGTEDGIK EPTATFSACF GAAFIMLHPT KYAAMLAEKM KSQGATGWLV NTGWSGGSYG VGNRIKLAYT RKIIDAIHSG SLLKANYKKT
601: EIFGFEIPTE IEGIPSEILD PVNSWSDKKA HKDTLVKLGG LFKKNFEVFA NHKIGVDGKL TEEILAAGPI F

Arabidopsis Description

PCKAPhosphoenolpyruvate carboxykinase (ATP)-like protein [Source:UniProtKB/TrEMBL;Acc:Q0WWL8]
SUBAcon: [cytosol]

Hydropathy Plot

About CropPAL

The Protein Annotated Locations Database (CropPAL) houses large scale proteomic and GFP localization data from published experimental studies in Soybean (Glycine max), Maize (Zea mays), Wheat (Triticum aestivum), Barley (Hordeum vulgare), Rice (Oryza sativa), Field mustard (Brassica rapa), Canola (Brassica napus), Sorghum (Sorghum bicolor), Potato (Solanum tuberosum), Tomato (Solanum lycopersicum), Banana (Musa acuminata) and Wine grape (Vitis vinifera) as well as precomputed predictions for protein subcellular localizations using protein sequences.