CropPAL

Subcellular Localization

min:

: max

Winner_takes_all: nucleus

Predictor Summary:

nucleus 3
cytosol 3
mitochondrion 1

Predictors

GFP

MS/MS

Papers

Winner Takes All:	nucleus
Any Predictor:	cytosol, mitochondrion, nucleus
BaCelLo:	nucleus
EpiLoc:	cytosol
MultiLoc:	cytosol
Plant-mPloc:	nucleus
PProwler:	mitochondrion
WoLF PSORT:	nucleus
YLoc:	cytosol

nucleus: 21132161

msms PMID: 21132161 doi

B Cooper, KB Campbell, J Feng, WM Garrett, R Frederick

Soybean Genomics and Improvement Laboratory, USDA-ARS, Beltsville, MD 20705, USA. bret.cooper@ars.usda.gov

PPI

Inferred distinct locusB in Crop

locusB	locations
KRG98502
KRG98507
KRH46360
KRH46364

Inferred from Arabidopsis experimental PPI

Ath locusA	locusB	Ath locusB	Paper
AT1G18550.1	KRG98502	AT4G26630.1	25387881
AT1G18550.1	KRG98507	AT4G26630.1	25387881
AT1G18550.1	KRH46360	AT4G26630.1	25387881
AT1G18550.1	KRH46364	AT4G26630.1	25387881

Homology

Paralog

locus	Identity	Homology Identity

Ortholog

locus	Homology Species	Location	Identity	Homology Identity
KRH56713	Soybean	cytosol, plastid	80.0	67.76
KRH03839	Soybean	nucleus	79.03	66.22
VIT_17s0000g04330.t01	Wine grape	cytosol	77.74	63.34
GSMUA_Achr6P13660_001	Banana	cytosol	69.19	62.9
Zm00001d042644_P001	Maize	cytosol	31.94	62.66
Solyc03g114380.2.1	Tomato	cytosol	74.68	59.44
Zm00001d048931_P001	Maize	nucleus	33.23	58.86
AT1G18550.1	Thale cress	nucleus	68.23	58.34
PGSC0003DMT400062969	Potato	cytosol	75.32	58.3
CDY26149	Canola	nucleus	67.42	58.14
CDY19223	Canola	nucleus	67.42	58.06
Bra025894.1-P	Field mustard	nucleus	64.35	56.92
Os01t0605500-00	Rice	plastid	67.74	54.12
HORVU3Hr1G052930.1	Barley	plastid	66.29	53.45
TraesCS3D01G218300.1	Wheat	plastid	66.77	53.28
TraesCS3A01G214700.1	Wheat	plastid	66.61	52.81
TraesCS3B01G246600.1	Wheat	plastid	66.13	52.56
EES03243	Sorghum	mitochondrion	63.71	50.71
Zm00001d044420_P001	Maize	mitochondrion	63.39	50.26
KRH44359	Soybean	cytosol	25.0	23.0
KRH47037	Soybean	cytosol	24.84	22.85
KRH09867	Soybean	cytosol	25.16	22.25
KRH23459	Soybean	cytosol	25.0	22.11
KRG93660	Soybean	mitochondrion	9.68	21.58
KRH39281	Soybean	cytosol	23.55	21.57
KRH48398	Soybean	cytosol	23.71	21.24
KRH02264	Soybean	cytosol	26.13	19.88
KRH49096	Soybean	plastid	20.48	19.78
KRH50824	Soybean	cytosol	25.97	19.78
KRG99933	Soybean	plastid	20.32	19.63
KRH37102	Soybean	cytosol	25.48	18.08
KRH12061	Soybean	cytosol	25.32	18.0
KRH38442	Soybean	cytosol	15.97	14.41
KRH08928	Soybean	cytosol	16.45	14.21

Protein Annotations

EntrezGene:100815524	MapMan:20.1.3.7	Gene3D:3.40.850.10	EMBL:ACUP02002468	ncoils:Coil	EnsemblPlantsGene:GLYMA_04G145200
GO:GO:0000166	GO:GO:0003674	GO:GO:0003774	GO:GO:0003777	GO:GO:0003824	GO:GO:0005488
GO:GO:0005515	GO:GO:0005524	GO:GO:0005575	GO:GO:0005622	GO:GO:0005623	GO:GO:0005856
GO:GO:0005871	GO:GO:0005874	GO:GO:0007018	GO:GO:0008017	GO:GO:0008150	GO:GO:0009987
GO:GO:0016787	GO:GO:0016887	UniProt:I1N1P2	InterPro:IPR001752	InterPro:IPR036961	EnsemblPlants:KRH62967
ProteinID:KRH62967	ProteinID:KRH62967.1	InterPro:Kinesin-like_fam	InterPro:Kinesin_motor_CS	InterPro:Kinesin_motor_dom	InterPro:Kinesin_motor_dom_sf
InterPro:P-loop_NTPase	PFAM:PF00225	PRINTS:PR00380	ScanProsite:PS00411	PFscan:PS50067	PANTHER:PTHR24115
PANTHER:PTHR24115:SF557	SMART:SM00129	SUPFAM:SSF52540	UniParc:UPI000233EDFA	SEG:seg	:

Description

hypothetical protein

Coordinates

chr4:-:26615737..26619972

Molecular Weight (calculated)

68787.5 Da

IEP (calculated)

10.430

GRAVY (calculated)

-0.532

Length

620 amino acids

Sequence
(BLAST)

001: MPASTRSQTV PNESNSEHDA LKEKMKSLLA LYEQHKQKHG AAAAAPPPSK NPSLKTGTRI LVFVRVRPLA KKEMEAGSRC CVRIVDRRDV YLTEFASEKD
101: YLRLKRLRGR HFAFDASFPD SATQQDVYST TTSELVEAVL QGRNGSVFCY GATGAGKTYT MLGTVESPGV MVLAIKDLFN KIRMRSYDGN HAVHLSYLEV
201: YNETVRDLLS PGRPLVLRED KQGIVAAGLT QYRAYSTDEV MALLQQGNRS RTTEPTRANE TSSRSHAILQ VVVEYRVRDA AMNIIKKMGK LSLIDLAGSE
301: RALATDQRTV RSLEGANINR SLLALSSCIN ALVEGKKHIP YRNSKLTQLL KDSLGGSCNT VMIANISPSN LAFGETQNTL HWADRAKEIR TKAINANEDL
401: LPVPETETDQ AKLVLELQKE NRELRMQLAR QHQKIMTLQA HSSAPPTPPS ATSLLSTPPT SAPPSEKRRT RPSLLAATHF TPEAKNKGDE VAVTIRTLQQ
501: RVKALEAELE RVKKDYSLQL KQKDDVIREL SQKGGKKVVA TRASLRPKEA NTGELKSPSY RFWSPVQTAK KRSFWDITTA NSPSVTTLNG RKTRSHLISE
601: PTAHPSMLLQ PGFARKKGDI

Best Arabidopsis Sequence Match ( AT1G18550.1 )
(BLAST)

001: MPVSTRSKVM KQERNEQENT NLNLPLRNPH QGLKEKMRAL TLLYEQQKRA SFSLRNPNHN QSPKPEDQRF KTQLLDSSKK GDRFHRLDGK DSTFVEEDTK
101: ENNVFEADRI FGVSSVPVKP SGVIRKLSMG NGARNVSEAE KLESLNASVS RILVFVRLRP MGKKERENGS RCCVKVLNKR DVYLTEFTNE NDYLRLKRLR
201: VRHFTFDSSF PETTTQQEVY STTTGDLVEA VLEGRNGSVF CYGATGAGKT YTMLGTMENP GVMVLAIKDL FAKVRQRSLD GNHVVHLSYL EVYNETVRDL
301: LSPGRPLILR EDKQGIVAAG LTQYRAYSTD EVMALLQRGN QNRTTEPTRC NETSSRSHAI LQVIVEYKTR DASMNIISRV GKLSLIDLAG SERALATDQR
401: TLRSLEGANI NRSLLALSSC INALVEGKKH IPYRNSKLTQ LLKDSLGGSC NTVMIANISP SSQSFGETQN TLHWADRAKE IRVKECEVNE EVVQVGEEEG
501: ADQAKLLLEL QKENSELRVQ LAKQQQKLLT LQAENIAAAN NNNNISLTPP SISSLMTPPS ALTAQQKKKP RHSLLSGTCF TPESLKRTKA EEAVKELQLT
601: VKALKMEMER MKREHGLQMK KQKDELMKDL CSRKSEKTPE RCKETRRIVT RGSLRPKEKE KELKSPSHRF ASPVAAAKKR SFWDITVANT SPALDRRKTR
701: SHGLVHQEAP SKLLQPGFAR PHMKH

Arabidopsis Description

KIN8AKinesin-like protein KIN-8A [Source:UniProtKB/Swiss-Prot;Acc:F4ICA0]
SUBAcon: [nucleus]

Hydropathy Plot

About CropPAL

The Protein Annotated Locations Database (CropPAL) houses large scale proteomic and GFP localization data from published experimental studies in Soybean (Glycine max), Maize (Zea mays), Wheat (Triticum aestivum), Barley (Hordeum vulgare), Rice (Oryza sativa), Field mustard (Brassica rapa), Canola (Brassica napus), Sorghum (Sorghum bicolor), Potato (Solanum tuberosum), Tomato (Solanum lycopersicum), Banana (Musa acuminata) and Wine grape (Vitis vinifera) as well as precomputed predictions for protein subcellular localizations using protein sequences.