Factsheet for Os02t0538000-01

Subcellular Localization

min:

: max

Winner_takes_all: plasma membrane

Predictor Summary:

plastid 3
cytosol 2
mitochondrion 5

Predictors

GFP

MS/MS

Papers

plasma membrane: 27800704

msms PMID: 27800704 doi

J Cao, C Yang, L Li, L Jiang, Y Wu, C Wu, Q Bu, G Xia, X Liu, Y Luo, J Liu

Huai'an Institute of Agricultural Sciences, Huai'an, 223001, China., Institute of Analytical Chemistry and Synthetic and Functional Biomolecules Center, College of Chemistry and Molecular Engineering, Peking University, Beijing, 100871, China; and., Northeast Institute of Geography and Agroecology, Key Laboratory of Soybean Molecular Design Breeding, Chinese Academy of Sciences, Harbin, 150081, China., State Key Laboratory of Plant Genomics, Institute of Microbiology, Chinese Academy of Sciences, Beijing, 100101, China.

PPI

Inferred distinct locusB in Crop

locusB	locations
Os01t0921600-01

Inferred from Arabidopsis experimental PPI

Ath locusA	locusB	Ath locusB	Paper
AT2G04842.1	Os01t0921600-01	AT5G40930.1	21848659

Homology

Paralog

locus	Identity	Homology Identity

Ortholog

locus	Homology Species	Location	Identity	Homology Identity
KXG36582	Sorghum	plastid	83.98	85.11
TraesCS6D01G262800.1	Wheat	plastid	83.09	83.96
TraesCS6A01G282300.1	Wheat	plastid	82.94	83.81
Zm00001d021962_P001	Maize	plastid	77.6	82.36
TraesCS6B01G310700.1	Wheat	plastid	78.64	81.92
HORVU6Hr1G070790.5	Barley	plastid	81.9	79.65
GSMUA_Achr4P01570_001	Banana	plastid	75.52	76.2
KRH17875	Soybean	mitochondrion	28.49	75.59
KRG96128	Soybean	nucleus	72.11	74.54
Bra013193.1-P	Field mustard	plastid	71.96	74.39
CDY50043	Canola	plastid	71.51	73.93
AT2G04842.1	Thale cress	plastid	71.07	73.69
CDX93065	Canola	plastid	71.96	73.6
VIT_12s0057g00400.t01	Wine grape	plastid	72.85	72.1
Solyc07g048080.2.1	Tomato	nucleus	71.66	71.77
PGSC0003DMT400015710	Potato	mitochondrion, plastid	70.92	71.13
Os08t0295300-01	Rice	golgi, plasma membrane	34.27	31.01

Protein Annotations

KEGG:00970+6.1.1.3	MapMan:17.2.19	Gene3D:3.30.54.20	Gene3D:3.30.930.10	Gene3D:3.30.980.10	Gene3D:3.40.50.800
EntrezGene:4329580	EMBL:AK058738	InterPro:Anticodon-bd	InterPro:Anticodon-bd_dom_sf	ProteinID:BAD27919.1	ProteinID:BAD28830.1
ProteinID:BAF08966.1	ProteinID:BAS79082.1	ncoils:Coil	ProteinID:EEE57138.1	GO:GO:0000003	GO:GO:0000166
GO:GO:0003674	GO:GO:0003824	GO:GO:0004812	GO:GO:0004829	GO:GO:0005488	GO:GO:0005524
GO:GO:0005575	GO:GO:0005622	GO:GO:0005623	GO:GO:0005737	GO:GO:0005739	GO:GO:0006139
GO:GO:0006412	GO:GO:0006418	GO:GO:0006435	GO:GO:0007275	GO:GO:0008150	GO:GO:0008152
GO:GO:0009058	GO:GO:0009507	GO:GO:0009536	GO:GO:0009570	GO:GO:0009790	GO:GO:0009791
GO:GO:0009793	GO:GO:0009987	GO:GO:0019538	GO:GO:0043039	InterPro:IPR006195	InterPro:IPR036621
HAMAP:MF_00184	EnsemblPlantsGene:Os02g0538000	EnsemblPlants:Os02t0538000-01	PFAM:PF00587	PFAM:PF03129	PFAM:PF07973
PRINTS:PR01047	PFscan:PS50862	PANTHER:PTHR11451	PANTHER:PTHR11451:SF44	UniProt:Q6ER90	SMART:SM00863
SUPFAM:SSF52954	SUPFAM:SSF55186	SUPFAM:SSF55681	TIGRFAMs:TIGR00418	InterPro:Thr-tRNA-ligase_IIa	InterPro:Thr/Ala-tRNA-synth_IIc_edit
InterPro:ThrRS_core	UniParc:UPI00003CE556	RefSeq:XP_015625570.1	InterPro:aa-tRNA-synt_IIb	InterPro:aa-tRNA-synth_II	SEG:seg
InterPro:tRNA_SAD	:	:	:	:	:

Description

Lethal albinic seedlingThreonyl-tRNA synthetase, Chloroplast biogenesis and chloroplast ribosomal system construction (Os02t0538000-01)

Coordinates

chr2:-:19924420..19928340

Molecular Weight (calculated)

76941.8 Da

IEP (calculated)

6.878

GRAVY (calculated)

-0.463

Length

674 amino acids

Sequence
(BLAST)

001: MAAAVSAPLL RFHHHHRCLL RHSSSPRRYP AGFSRGLSSV SFGLPRSTTT AARSTASPSA PSAAAAEATD AASAQAGSDG KGDGIGEEER VVLPTNESSD
101: RLLRIRHTCA HVMAMAVQKL FPNSKVTIGP WIDNGFYYDF DMEPLTDKDL KRIKKEMDRI IRKNLPLVRE EVSREEAQKR IEALNEPYKL EILEGIKEDP
201: ITIYHIGEEW WDLCAGPHVE STGKIDRRAV ELESVAGAYW RGDESNQMLQ RIYGTAWETE DQLKAYIHFK EEAKRRDHRR LGLDLDLFSI QEDAGGGLVF
301: WHPKGAIIRH VLEDSWKQIH LQRGYDLLYT PHVAKAELWK VSGHIDFYKE NMYNQMDVED ELYQLRPMNC PYHILVYKRK LHSYRDFPIR VAELGTVYRY
401: ELSGSLHGLF RVRGFTQDDA HIFCLEDQIK DEIRGVLDLT EQILGQFGFR YYEINLSTRP EKSVGSDDIW EKATLALKDA LEDKGWEYKV DEGGGAFYGP
501: KIDLKIEDAL GRKWQCSTVQ VDFNLPERFD ITYVNSNSEK KRPIMIHRAI LGSLERFFGV LIEHYAGDFP LWLAPIQARI LPVTDNELQY CNEVASELKS
601: KGIRAEVCHG ERLPKLIRNA ETKKVPLMAV VGPKEVQART LTIRSRHSGE IGTMPVDEFF RRIQLAIANK SSSL

Best Arabidopsis Sequence Match ( AT2G04842.2 )
(BLAST)

001: MASSHSLLFS SSFLSKPSSF TSSLRRFVYL PTRQFWPRQR HGFSTVFAVA TEPAISSSGP KKAEPSTVVL PSNESSDKLL KIRHTCAHVM AMAVQKLFPD
101: AKVTIGPWID NGFYYDFDME PLTDKDLKRI KKEMDRIISR NLPLLREEVS REEAKKRIMA INEPYKMEIL DGIKEEPITV YHIGNEWWDL CAGPHVETTG
201: KINKKAVELE SVAGAYWRGD EKRQMLQRIY GTAWESEEQL KAYLHFKEEA KRRDHRRIGQ DLDLFSIQDE AGGGLVFWHP KGAIVRNIIE ESWKKMHVEH
301: GYDLIYTPHV AKADLWKISG HLDFYRENMY DQMEIEDELY QLRPMNCPYH ILLYQRKRQS YRDLPIRVAE LGTVYRYELS GSLHGLFRVR GFTQDDAHIF
401: CLEDQIKDEI RGVLDLTEEI LSRFGFNKYE VNLSTRPEKS VGGDDIWEKA TCALRDALDD KGWSYEVDEG GGAFYGPKID LKIEDALGRK WQCSTIQVDF
501: NLPQRFDITY VDTNSDKKRP IMIHRAVLGS LERFFGVLIE HYAGDFPLWL SPVQVRVLPV TDNQLEFCKE VSKKLRACGV RAELCHGERL PKLIRNAETQ
601: KIPLMAVVGP KEVETGTVTV RSRFGGELGT IPVDDLINKI NIAVETRTAL

Arabidopsis Description

EMB2761Threonine--tRNA ligase, chloroplastic/mitochondrial 2 [Source:UniProtKB/Swiss-Prot;Acc:F4IFC5]
SUBAcon: [plastid]

Hydropathy Plot

About CropPAL

The Protein Annotated Locations Database (CropPAL) houses large scale proteomic and GFP localization data from published experimental studies in Soybean (Glycine max), Maize (Zea mays), Wheat (Triticum aestivum), Barley (Hordeum vulgare), Rice (Oryza sativa), Field mustard (Brassica rapa), Canola (Brassica napus), Sorghum (Sorghum bicolor), Potato (Solanum tuberosum), Tomato (Solanum lycopersicum), Banana (Musa acuminata) and Wine grape (Vitis vinifera) as well as precomputed predictions for protein subcellular localizations using protein sequences.