Factsheet for Os08t0295300-01

Subcellular Localization

min:

: max

Winner_takes_all: plasma membrane, golgi

Predictor Summary:

plastid 1
cytosol 2
mitochondrion 7

Predictors

GFP

MS/MS

Papers

golgi: 25047511
plasma membrane: 27800704

msms PMID: 27800704 doi

J Cao, C Yang, L Li, L Jiang, Y Wu, C Wu, Q Bu, G Xia, X Liu, Y Luo, J Liu

Huai'an Institute of Agricultural Sciences, Huai'an, 223001, China., Institute of Analytical Chemistry and Synthetic and Functional Biomolecules Center, College of Chemistry and Molecular Engineering, Peking University, Beijing, 100871, China; and., Northeast Institute of Geography and Agroecology, Key Laboratory of Soybean Molecular Design Breeding, Chinese Academy of Sciences, Harbin, 150081, China., State Key Laboratory of Plant Genomics, Institute of Microbiology, Chinese Academy of Sciences, Beijing, 100101, China.

msms PMID: 25047511 doi

Y Nie, F Huang, S Dong, L Li, P Gao, H Zhao, Y Wang, S Han

Beijing Key Laboratory of Gene Resource and Molecular Development, Beijing Normal University, College of Life Sciences, Beijing, P. R. China.

PPI

No PPI Data

Homology

Paralog

locus	Identity	Homology Identity

Ortholog

locus	Homology Species	Location	Identity	Homology Identity
TraesCS3B01G080400.1	Wheat	golgi, unclear	82.42	86.97
TraesCS3D01G066900.1	Wheat	mitochondrion	81.61	85.88
Zm00001d032667_P005	Maize	mitochondrion	84.7	85.62
TraesCS3A01G067300.1	Wheat	mitochondrion	81.34	85.59
EER91117	Sorghum	mitochondrion	83.62	84.3
GSMUA_Achr1P18800_001	Banana	cytosol	58.12	83.59
HORVU3Hr1G012410.6	Barley	mitochondrion	80.0	82.09
HORVU1Hr1G057910.4	Barley	cytosol	42.68	79.9
Zm00001d027762_P001	Maize	cytosol	23.36	79.82
TraesCS1D01G236900.1	Wheat	cytosol, mitochondrion	64.3	78.65
TraesCS1B01G248400.1	Wheat	cytosol	62.28	78.64
TraesCS1A01G235200.1	Wheat	cytosol	72.08	78.28
Zm00001d013607_P003	Maize	mitochondrion	83.76	76.28
VIT_01s0026g00340.t01	Wine grape	cytosol	70.2	76.24
VIT_19s0015g00470.t01	Wine grape	cytosol	70.07	76.09
Solyc08g074550.2.1	Tomato	plastid	67.65	74.56
CDY19754	Canola	cytosol	60.27	73.73
KRH69521	Soybean	endoplasmic reticulum, nucleus	68.99	72.39
KRH74628	Soybean	endoplasmic reticulum	69.53	72.25
Solyc05g005800.2.1	Tomato	plastid	69.13	71.03
CDX88245	Canola	mitochondrion	66.58	69.08
Bra009922.1-P	Field mustard	mitochondrion	66.58	69.08
Bra036596.1-P	Field mustard	mitochondrion	65.23	68.74
CDX80596	Canola	extracellular	65.77	68.63
PGSC0003DMT400097200	Potato	cytosol, mitochondrion	37.85	68.28
AT5G26830.1	Thale cress	mitochondrion	64.43	67.7
CDX87030	Canola	mitochondrion, plastid	64.56	66.9
PGSC0003DMT400076047	Potato	cytosol	17.72	65.67
Bra031021.1-P	Field mustard	cytosol	49.8	64.3
CDY21715	Canola	cytosol	53.42	63.38
AT1G17960.1	Thale cress	cytosol	37.32	60.7
CDY21726	Canola	cytosol	33.15	56.65
Bra031009.1-P	Field mustard	cytosol	28.32	56.57
CDX81854	Canola	cytosol	32.48	55.89
AT1G18130.1	Thale cress	cytosol	12.08	54.55
Solyc07g064750.1.1	Tomato	cytosol	12.48	38.27
Os02t0538000-01	Rice	plasma membrane	31.01	34.27

Protein Annotations

KEGG:00970+6.1.1.3	MapMan:17.2.19	Gene3D:3.10.20.30	Gene3D:3.30.930.10	Gene3D:3.30.980.10	Gene3D:3.40.50.800
EntrezGene:4345200	EMBL:AK070378	InterPro:Anticodon-bd	InterPro:Anticodon-bd_dom_sf	ProteinID:BAD01219.1	ProteinID:BAD01414.1
ProteinID:BAF23389.1	ProteinID:BAT04781.1	ProteinID:BAT04782.1	InterPro:Beta-grasp_dom_sf	GO:GO:0000166	GO:GO:0003674
GO:GO:0003824	GO:GO:0004812	GO:GO:0004829	GO:GO:0005488	GO:GO:0005524	GO:GO:0005575
GO:GO:0005622	GO:GO:0005623	GO:GO:0005737	GO:GO:0006139	GO:GO:0006412	GO:GO:0006418
GO:GO:0006435	GO:GO:0008150	GO:GO:0008152	GO:GO:0009058	GO:GO:0009987	GO:GO:0016874
GO:GO:0019538	GO:GO:0043039	InterPro:IPR006195	InterPro:IPR012675	InterPro:IPR036621	HAMAP:MF_00184
EnsemblPlantsGene:Os08g0295300	EnsemblPlants:Os08t0295300-01	PFAM:PF00587	PFAM:PF02824	PFAM:PF03129	PFAM:PF07973
PRINTS:PR01047	PFscan:PS50862	PANTHER:PTHR11451	PANTHER:PTHR11451:SF46	UniProt:Q6Z0N5	SMART:SM00863
SUPFAM:SSF52954	SUPFAM:SSF55186	SUPFAM:SSF55681	SUPFAM:SSF81271	InterPro:TGS	InterPro:TGS-like
TIGRFAMs:TIGR00418	InterPro:Thr-tRNA-ligase_IIa	InterPro:Thr/Ala-tRNA-synth_IIc_edit	InterPro:ThrRS_core	UniParc:UPI0000212F95	RefSeq:XP_015650707.1
InterPro:aa-tRNA-synt_IIb	InterPro:aa-tRNA-synth_II	SEG:seg	InterPro:tRNA_SAD	:	:

Description

Similar to Threonyl-tRNA synthetase, cytoplasmic (EC 6.1.1.3) (Threonine--tRNA ligase) (ThrRS). (Os08t0295300-01);Similar to Threonyl-tRNA synthetase (Fragment). (Os08t0295300-02)

Coordinates

chr8:-:11887897..11897477

Molecular Weight (calculated)

84950.8 Da

IEP (calculated)

8.051

GRAVY (calculated)

-0.406

Length

745 amino acids

Sequence
(BLAST)

001: MLVCLRRRGL SLLRQRPPRP FPPLPRPRSS PPRVASLRPL SAAAMGENSA AAAGKGKEAK GKATTSASAS APNVEPDVAY LEAVTQKRIR QFEEIQAKQA
101: LERLNIGGEP IRITLPDGAV KDGKKWISTP MDIAKEISSG LANSCLIAQV NGTLWDMTRP LEGDCELKLF KFDSNEGRDT FWHSSAHILG ESLERAYGCK
201: LCIGPCTTRG EGFYYDAYYN DLTLNETHFG IIDAQAQKAV AEKQPFERIE VSRAEALEMF AENKFKVEII NELPEDKTIT VYRCGPLVDL CRGPHIPNTS
301: FVKAFACLKA SSSYWRGKAD RESLQRVYGI SFPDSKRLKE YKHLLEEAKK RDHRLLGQTQ DLFFFHQLSP GSCFFLPHGA IIYNKLMDFL RQQYRDRGYQ
401: EVLSPNIYNM QLWETSGHAA NYKENMFVFE IEKQEFGLKP MNCPGHCLMF EHRVRSYREL PLRMADFGVL HRNELSGALT GLTRVRRFQQ DDAHIFCRES
501: QIKDEVKAVL DFINYVYEIF GFKYELELST RPEKYLGDIE TWNKAEQQLT EALNEFGKPW QINEGDGAFY GPKIDIGVFD ALKRKFQCAT LQLDFQLPLR
601: FKLTYSAEDE AKLERPVMIH RAILGSVERM FAILLEHYNG KWPLWLSPRQ AIVCSISSNS VEYAKQVRAR IHEAGFHVAI DETDRTIQKK VREAQLAQFN
701: YILVVGAQEA ETGQVSVRVR DKAELATVSI DDIITRFKEE VAAYK

Best Arabidopsis Sequence Match ( AT5G26830.1 )
(BLAST)

001: MLLRLTARSI RRFTTSSSSL PLLSSSSFCT VPTMAANHPK DEAYLSAVIP KRIKLFEQIQ ANQLENLKSL PHDPIKVTLP DGNVKEGKKW ETTPMDIAAQ
101: ISKGLANSAL ISAVDDVLWD MNRPLEGDCK LELFKFDSDK GRDTLWHSSA HILGQALEQE YGCQLCIGPC TTRGEGFYYD GFYGELGLSD NHFPSIEAGA
201: AKAAKEAQPF ERIEVTKDQA LEMFSENNFK VELINGLPAD MTITVYRCGP LVDLCRGPHI PNTSFVKAFK CLRASSAYWK GDKDRESLQR VYGISYPDQK
301: QLKKYLQFLE EAKKYDHRLL GQKQELFFSH QLSPGSYFFL PLGTRVYNRL MDFIKNQYWH RGYTEVITPN MYNMELWQTS GHADNYKDNM FTFNIEKQEF
401: GLKPMNCPGH CLIFQHRVRS YRELPMRLAD FGVLHRNEAS GALSGLTRVR RFQQDDAHIF CTTEQVKGEV QGVLEFIDYV YKVFGFTYEL KLSTRPEKYL
501: GDLETWDKAE ADLKEAIEAF GKPLVLNEGD GAFYGPKIDI TVSDAMNRKF QCATLQLDFQ LPIRFNLEYA AEDEAKKSRP VMIHRAVLGS VERMFAILLE
601: HYKGKWPFWI SPRQAIVCPI SEKSQQYAEK VQKQIKDAGF YVDADLTDRK IDKKVREAQL AQYNYILVVG ETEAATGQVS VRVRDNAAHS VKSIEDLLEE
701: FKAKTAEFV

Arabidopsis Description

THRRSThreonine--tRNA ligase, mitochondrial 1 [Source:UniProtKB/Swiss-Prot;Acc:O04630]
SUBAcon: [mitochondrion]

Hydropathy Plot

About CropPAL

The Protein Annotated Locations Database (CropPAL) houses large scale proteomic and GFP localization data from published experimental studies in Soybean (Glycine max), Maize (Zea mays), Wheat (Triticum aestivum), Barley (Hordeum vulgare), Rice (Oryza sativa), Field mustard (Brassica rapa), Canola (Brassica napus), Sorghum (Sorghum bicolor), Potato (Solanum tuberosum), Tomato (Solanum lycopersicum), Banana (Musa acuminata) and Wine grape (Vitis vinifera) as well as precomputed predictions for protein subcellular localizations using protein sequences.