CropPAL

Subcellular Localization

min:

: max

Winner_takes_all: nucleus, endoplasmic reticulum

Predictor Summary:

plastid 3
cytosol 2
mitochondrion 6

Predictors

GFP

MS/MS

Papers

nucleus: 21132161
endoplasmic reticulum: 27224218

msms PMID: 21132161 doi

B Cooper, KB Campbell, J Feng, WM Garrett, R Frederick

Soybean Genomics and Improvement Laboratory, USDA-ARS, Beltsville, MD 20705, USA. bret.cooper@ars.usda.gov

msms PMID: 27224218 doi

X Wang, S Komatsu

Graduate School of Life and Environmental Sciences, University of Tsukuba , Tsukuba 305-8572, Japan., National Institute of Crop Science, National Agriculture and Food Research Organization , Tsukuba 305-8518, Japan.

PPI

Inferred distinct locusB in Crop

locusB	locations
KRH03376
KRH54462
KRH57009
KRH63457

Inferred from Arabidopsis experimental PPI

Ath locusA	locusB	Ath locusB	Paper
AT5G26830.1	KRH03376	AT3G48100.1	18642946
AT5G26830.1	KRH54462	AT3G48100.1	18642946
AT5G26830.1	KRH57009	AT3G48100.1	18642946
AT5G26830.1	KRH63457	AT3G48100.1	18642946

Homology

Paralog

locus	Identity	Homology Identity

Ortholog

locus	Homology Species	Location	Identity	Homology Identity
KRH74628	Soybean	endoplasmic reticulum	96.06	95.12
VIT_01s0026g00340.t01	Wine grape	cytosol	77.89	80.61
VIT_19s0015g00470.t01	Wine grape	cytosol	77.46	80.17
CDY19754	Canola	cytosol	67.61	78.82
CDX88245	Canola	mitochondrion	75.21	74.37
Bra009922.1-P	Field mustard	mitochondrion	75.21	74.37
Bra036596.1-P	Field mustard	mitochondrion	73.52	73.83
AT5G26830.1	Thale cress	mitochondrion	73.52	73.62
CDX80596	Canola	extracellular	73.52	73.11
CDX87030	Canola	mitochondrion, plastid	73.1	72.18
HORVU1Hr1G057910.4	Barley	cytosol	40.14	71.61
TraesCS3D01G066900.1	Wheat	mitochondrion	70.56	70.76
TraesCS3A01G067300.1	Wheat	mitochondrion	70.42	70.62
TraesCS3B01G080400.1	Wheat	golgi, unclear	70.14	70.54
TraesCS1B01G248400.1	Wheat	cytosol	57.75	69.49
Zm00001d027762_P001	Maize	cytosol	21.27	69.27
TraesCS1A01G235200.1	Wheat	cytosol	66.9	69.24
Os08t0295300-01	Rice	golgi, plasma membrane	72.39	68.99
HORVU3Hr1G012410.6	Barley	mitochondrion	70.42	68.87
Zm00001d032667_P005	Maize	mitochondrion	71.27	68.66
EER91117	Sorghum	mitochondrion	70.42	67.66
TraesCS1D01G236900.1	Wheat	cytosol, mitochondrion	58.03	67.65
PGSC0003DMT400097200	Potato	cytosol, mitochondrion	39.15	67.31
Bra031021.1-P	Field mustard	cytosol	54.65	67.24
CDY21715	Canola	cytosol	58.73	66.4
AT1G17960.1	Thale cress	cytosol	41.83	64.85
AT1G18130.1	Thale cress	cytosol	14.93	64.24
Zm00001d013607_P003	Maize	mitochondrion	70.14	60.88
Bra031009.1-P	Field mustard	cytosol	30.0	57.1
CDY21726	Canola	cytosol	34.79	56.65
CDX81854	Canola	cytosol	34.37	56.35
GSMUA_Achr1P18790_001	Banana	cytosol	18.73	48.72
Solyc07g064750.1.1	Tomato	cytosol	13.52	39.51
KRH17875	Soybean	mitochondrion	13.66	38.19
KRG96128	Soybean	nucleus	32.82	35.74

Protein Annotations

KEGG:00970+6.1.1.3	EntrezGene:100802879	MapMan:17.2.19	Gene3D:3.10.20.30	Gene3D:3.30.930.10	Gene3D:3.30.980.10
Gene3D:3.40.50.800	EMBL:ACUP02000720	InterPro:Anticodon-bd	InterPro:Anticodon-bd_dom_sf	InterPro:Beta-grasp_dom_sf	EnsemblPlantsGene:GLYMA_02G032800
GO:GO:0000166	GO:GO:0003674	GO:GO:0003824	GO:GO:0004812	GO:GO:0004829	GO:GO:0005488
GO:GO:0005524	GO:GO:0005575	GO:GO:0005622	GO:GO:0005623	GO:GO:0005737	GO:GO:0006139
GO:GO:0006412	GO:GO:0006418	GO:GO:0006435	GO:GO:0008150	GO:GO:0008152	GO:GO:0009058
GO:GO:0009987	GO:GO:0019538	GO:GO:0043039	UniProt:I1JC08	InterPro:IPR006195	InterPro:IPR012675
InterPro:IPR036621	EnsemblPlants:KRH69521	ProteinID:KRH69521	ProteinID:KRH69521.1	HAMAP:MF_00184	PFAM:PF00587
PFAM:PF02824	PFAM:PF03129	PFAM:PF07973	PRINTS:PR01047	PFscan:PS50862	PANTHER:PTHR11451
PANTHER:PTHR11451:SF46	SMART:SM00863	SUPFAM:SSF52954	SUPFAM:SSF55186	SUPFAM:SSF55681	SUPFAM:SSF81271
InterPro:TGS	InterPro:TGS-like	TIGRFAMs:TIGR00418	InterPro:Thr-tRNA-ligase_IIa	InterPro:Thr/Ala-tRNA-synth_IIc_edit	InterPro:ThrRS_core
UniParc:UPI0002337467	InterPro:aa-tRNA-synt_IIb	InterPro:aa-tRNA-synth_II	SEG:seg	InterPro:tRNA_SAD	:

Description

hypothetical protein

Coordinates

chr2:-:3029017..3040197

Molecular Weight (calculated)

81827.6 Da

IEP (calculated)

7.221

GRAVY (calculated)

-0.440

Length

710 amino acids

Sequence
(BLAST)

001: MLCSLIRFRR SAPSYHTLHS LFPTINRFSS SASAAMVAHA KDEAYLSATI PKRIRLFETI LAEQHTQRLS LSPDPIKVTL PDGSVKEAKK WHTTPLDVAR
101: EISKNLANSA LIAKVNGVLW DMTRPLEDDC QLQIFKFDDD EGRDTFWHSS AHILGQSLET EYGCKLCIGP CTTRGEGFYY DAFYGELGLN DDHFKQIEAG
201: ALKAVAEKQP FERIEVTRDQ ALEMFSDNKF KVEIINDLPA DKTITVYRCG PLVDLCRGPH IPNTSFVKAI ACLKASSAYW RGDKDRESLQ RVYGISYPDQ
301: KSLKEYLHRL EEAKKYDHRI LGVKQELILH HEWSPGSWFF LPQGTRIYNK LMDFIRNQYR DRGYQEVISP NVFNMELWVQ SGHAANYRED MFILEVDKQE
401: FGLKPMNCPG HCLMFKHRVR SYRELPLRFA DFGVLHRNEA SGALSGLTRV RRFQQDDAHI FCRESQIKDE VRNSLNFINY VYKIFGFTYE LKLSTRPEKY
501: LGDIATWDKA ESALKEALDD FGKPWQLNEG DGAFYGPKID ISVSDALGRK FQCATLQLDF QLPDRFKLEF SAEDEAKIER PVMIHRAILG SVERMFAILL
601: EHYKGKWPFW LSPRQAIVCP VSEKSQAYAL QVRDQIHQAG YYVDADTTDR KIQKKVREAQ LAQYNYILVV GEEEANTGQV SVRVRDLAEH KVMSIEKLLE
701: HFRDKAAAFE

Best Arabidopsis Sequence Match ( AT5G26830.1 )
(BLAST)

001: MLLRLTARSI RRFTTSSSSL PLLSSSSFCT VPTMAANHPK DEAYLSAVIP KRIKLFEQIQ ANQLENLKSL PHDPIKVTLP DGNVKEGKKW ETTPMDIAAQ
101: ISKGLANSAL ISAVDDVLWD MNRPLEGDCK LELFKFDSDK GRDTLWHSSA HILGQALEQE YGCQLCIGPC TTRGEGFYYD GFYGELGLSD NHFPSIEAGA
201: AKAAKEAQPF ERIEVTKDQA LEMFSENNFK VELINGLPAD MTITVYRCGP LVDLCRGPHI PNTSFVKAFK CLRASSAYWK GDKDRESLQR VYGISYPDQK
301: QLKKYLQFLE EAKKYDHRLL GQKQELFFSH QLSPGSYFFL PLGTRVYNRL MDFIKNQYWH RGYTEVITPN MYNMELWQTS GHADNYKDNM FTFNIEKQEF
401: GLKPMNCPGH CLIFQHRVRS YRELPMRLAD FGVLHRNEAS GALSGLTRVR RFQQDDAHIF CTTEQVKGEV QGVLEFIDYV YKVFGFTYEL KLSTRPEKYL
501: GDLETWDKAE ADLKEAIEAF GKPLVLNEGD GAFYGPKIDI TVSDAMNRKF QCATLQLDFQ LPIRFNLEYA AEDEAKKSRP VMIHRAVLGS VERMFAILLE
601: HYKGKWPFWI SPRQAIVCPI SEKSQQYAEK VQKQIKDAGF YVDADLTDRK IDKKVREAQL AQYNYILVVG ETEAATGQVS VRVRDNAAHS VKSIEDLLEE
701: FKAKTAEFV

Arabidopsis Description

THRRSThreonine--tRNA ligase, mitochondrial 1 [Source:UniProtKB/Swiss-Prot;Acc:O04630]
SUBAcon: [mitochondrion]

Hydropathy Plot

About CropPAL

The Protein Annotated Locations Database (CropPAL) houses large scale proteomic and GFP localization data from published experimental studies in Soybean (Glycine max), Maize (Zea mays), Wheat (Triticum aestivum), Barley (Hordeum vulgare), Rice (Oryza sativa), Field mustard (Brassica rapa), Canola (Brassica napus), Sorghum (Sorghum bicolor), Potato (Solanum tuberosum), Tomato (Solanum lycopersicum), Banana (Musa acuminata) and Wine grape (Vitis vinifera) as well as precomputed predictions for protein subcellular localizations using protein sequences.