CropPAL

Subcellular Localization

min:

: max

Winner_takes_all: endoplasmic reticulum

Predictor Summary:

plastid 4
cytosol 1
mitochondrion 6

Predictors

GFP

MS/MS

Papers

nucleus: 21132161
endoplasmic reticulum: 23041469
endoplasmic reticulum: 27224218
endoplasmic reticulum: 28499913

msms PMID: 21132161 doi

B Cooper, KB Campbell, J Feng, WM Garrett, R Frederick

Soybean Genomics and Improvement Laboratory, USDA-ARS, Beltsville, MD 20705, USA. bret.cooper@ars.usda.gov

msms PMID: 23041469 doi

S Komatsu, R Kuji, Y Nanjo, S Hiraga, K Furukawa

National Institute of Crop Science, Tsukuba 305-8518, Japan. skomatsu@affrc.go.jp

msms PMID: 28499913 doi

S Komatsu, X Wang, X Yin, Y Nanjo, H Ohyanagi, K Sakata

Department of Life Science and Informatics, Maebashi Institute of Technology, Maebashi 371-0816, Japan. Electronic address: ksakata@maebashi-it.ac.jp., Institute of Crop Science, National Agriculture and Food Research Organization, Tsukuba 305-8518, Japan., Institute of Crop Science, National Agriculture and Food Research Organization, Tsukuba 305-8518, Japan; Faculty of Life and Environmental Sciences, University of Tsukuba, Tsukuba 305-8572, Japan., Institute of Crop Science, National Agriculture and Food Research Organization, Tsukuba 305-8518, Japan; Faculty of Life and Environmental Sciences, University of Tsukuba, Tsukuba 305-8572, Japan. Electronic address: komatsu.setsuko.fu@u.tsukuba.ac.jp., King Abdullah University of Science and Technology (KAUST), Computational Bioscience Research Center (CBRC), Thuwal 23955-6900, Saudi Arabia.

msms PMID: 27224218 doi

X Wang, S Komatsu

Graduate School of Life and Environmental Sciences, University of Tsukuba , Tsukuba 305-8572, Japan., National Institute of Crop Science, National Agriculture and Food Research Organization , Tsukuba 305-8518, Japan.

PPI

Inferred distinct locusB in Crop

locusB	locations
KRH03376
KRH54462
KRH57009
KRH63457

Inferred from Arabidopsis experimental PPI

Ath locusA	locusB	Ath locusB	Paper
AT5G26830.1	KRH03376	AT3G48100.1	18642946
AT5G26830.1	KRH54462	AT3G48100.1	18642946
AT5G26830.1	KRH57009	AT3G48100.1	18642946
AT5G26830.1	KRH63457	AT3G48100.1	18642946

Homology

Paralog

locus	Identity	Homology Identity

Ortholog

locus	Homology Species	Location	Identity	Homology Identity
KRH69521	Soybean	endoplasmic reticulum, nucleus	95.12	96.06
VIT_01s0026g00340.t01	Wine grape	cytosol	76.43	79.88
VIT_19s0015g00470.t01	Wine grape	cytosol	76.29	79.74
CDY19754	Canola	cytosol	66.53	78.33
CDX88245	Canola	mitochondrion	73.92	73.82
Bra009922.1-P	Field mustard	mitochondrion	73.92	73.82
Bra036596.1-P	Field mustard	mitochondrion	72.11	73.13
AT5G26830.1	Thale cress	mitochondrion	71.97	72.78
CDX80596	Canola	extracellular	72.38	72.69
CDX87030	Canola	mitochondrion, plastid	71.97	71.77
HORVU1Hr1G057910.4	Barley	cytosol	39.75	71.61
TraesCS3D01G066900.1	Wheat	mitochondrion	70.29	71.19
TraesCS3A01G067300.1	Wheat	mitochondrion	70.15	71.05
TraesCS3B01G080400.1	Wheat	golgi, unclear	69.87	70.96
Zm00001d027762_P001	Maize	cytosol	21.34	70.18
Os08t0295300-01	Rice	golgi, plasma membrane	72.25	69.53
TraesCS1B01G248400.1	Wheat	cytosol	57.18	69.49
HORVU3Hr1G012410.6	Barley	mitochondrion	70.01	69.15
TraesCS1A01G235200.1	Wheat	cytosol	65.97	68.95
Zm00001d032667_P005	Maize	mitochondrion	70.29	68.39
PGSC0003DMT400097200	Potato	cytosol, mitochondrion	39.19	68.04
EER91117	Sorghum	mitochondrion	69.87	67.79
TraesCS1D01G236900.1	Wheat	cytosol, mitochondrion	57.46	67.65
Bra031021.1-P	Field mustard	cytosol	53.84	66.9
CDY21715	Canola	cytosol	57.88	66.08
AT1G17960.1	Thale cress	cytosol	40.86	63.97
AT1G18130.1	Thale cress	cytosol	14.64	63.64
Zm00001d013607_P003	Maize	mitochondrion	69.6	61.0
Bra031009.1-P	Field mustard	cytosol	29.71	57.1
CDY21726	Canola	cytosol	34.45	56.65
CDX81854	Canola	cytosol	33.89	56.12
GSMUA_Achr1P18790_001	Banana	cytosol	18.55	48.72
Solyc07g064750.1.1	Tomato	cytosol	13.39	39.51
KRH17875	Soybean	mitochondrion	13.81	38.98
KRG96128	Soybean	nucleus	32.64	35.89

Protein Annotations

KEGG:00970+6.1.1.3	EntrezGene:100812652	MapMan:17.2.19	Gene3D:3.10.20.30	Gene3D:3.30.930.10	Gene3D:3.30.980.10
Gene3D:3.40.50.800	EMBL:ACUP02000052	InterPro:Anticodon-bd	InterPro:Anticodon-bd_dom_sf	InterPro:Beta-grasp_dom_sf	ncoils:Coil
EnsemblPlantsGene:GLYMA_01G032500	GO:GO:0000166	GO:GO:0003674	GO:GO:0003824	GO:GO:0004812	GO:GO:0004829
GO:GO:0005488	GO:GO:0005524	GO:GO:0005575	GO:GO:0005622	GO:GO:0005623	GO:GO:0005737
GO:GO:0006139	GO:GO:0006412	GO:GO:0006418	GO:GO:0006435	GO:GO:0008150	GO:GO:0008152
GO:GO:0009058	GO:GO:0009987	GO:GO:0019538	GO:GO:0043039	UniProt:I1J5B4	InterPro:IPR006195
InterPro:IPR012675	InterPro:IPR036621	EnsemblPlants:KRH74628	ProteinID:KRH74628	ProteinID:KRH74628.1	HAMAP:MF_00184
PFAM:PF00587	PFAM:PF02824	PFAM:PF03129	PFAM:PF07973	PRINTS:PR01047	PFscan:PS50862
PANTHER:PTHR11451	PANTHER:PTHR11451:SF46	SMART:SM00863	SUPFAM:SSF52954	SUPFAM:SSF55186	SUPFAM:SSF55681
SUPFAM:SSF81271	InterPro:TGS	InterPro:TGS-like	TIGRFAMs:TIGR00418	InterPro:Thr-tRNA-ligase_IIa	InterPro:Thr/Ala-tRNA-synth_IIc_edit
InterPro:ThrRS_core	UniParc:UPI0002337304	InterPro:aa-tRNA-synt_IIb	InterPro:aa-tRNA-synth_II	SEG:seg	InterPro:tRNA_SAD

Description

hypothetical protein

Coordinates

chr1:+:3415166..3425683

Molecular Weight (calculated)

82314.2 Da

IEP (calculated)

7.483

GRAVY (calculated)

-0.432

Length

717 amino acids

Sequence
(BLAST)

001: MLCSLIRIRR HAPFHHTLRS LLPINRFSSS SSSSASAAAA AAMVAHAKDE AYLSAAIPKR VRLFETIQAE QRTQRLSLSP DPIKVTLPDG NVKDAKKWLS
101: TPLDVAREIS KNLANSALIA KVNGVLWDMT RPLEDDCQLQ IFKFDDDEGR DTFWHSSAHI LGQSLETEYG CKLCIGPCTT RGEGFYYDAF YGDLGLNDDH
201: FKQIEAGALK AVAEKQPFER IEVTRDQALE MFSDNKFKIE IINDLPADKT ITVYRCGPLV DLCRGPHIPN TSFVKAIACL KASSAYWRGD KDRESLQRVY
301: GISYPDQKSL KEYLHRLEEA KKYDHRILGV KQELILHHEW SPGSWFFLPH GARIYNKLMD FIRNQYRDRG YQEVISPNVF NMDLWVQSGH AANYREDMFI
401: LEVDKQEFGL KPMNCPGHCL MFKHRVRSYR ELPLRFADFG VLHRNEASGA LSGLTRVRRF QQDDAHIFCR ESQIKDEVRN GLNFINYVYK IFGFTYELKL
501: STRPEKYLGD IATWDKAESA LKEALDDFGK PWQLNEGDGA FYGPKIDISV SDALSRKFQC ATLQLDFQLP DRFKLEFSAE DEAKIERPVM IHRAILGSVE
601: RMFAILLEHY KGKWPFWLSP RQAIVCPVSE KSQSYALQVR DQIHQAGYHV DADTTDRKIQ KKVREAQLAQ YNFILVVGEE EANSGQVSVR VRDLAEHKVM
701: SIEKLLEHFR DKTAAFE

Best Arabidopsis Sequence Match ( AT5G26830.1 )
(BLAST)

001: MLLRLTARSI RRFTTSSSSL PLLSSSSFCT VPTMAANHPK DEAYLSAVIP KRIKLFEQIQ ANQLENLKSL PHDPIKVTLP DGNVKEGKKW ETTPMDIAAQ
101: ISKGLANSAL ISAVDDVLWD MNRPLEGDCK LELFKFDSDK GRDTLWHSSA HILGQALEQE YGCQLCIGPC TTRGEGFYYD GFYGELGLSD NHFPSIEAGA
201: AKAAKEAQPF ERIEVTKDQA LEMFSENNFK VELINGLPAD MTITVYRCGP LVDLCRGPHI PNTSFVKAFK CLRASSAYWK GDKDRESLQR VYGISYPDQK
301: QLKKYLQFLE EAKKYDHRLL GQKQELFFSH QLSPGSYFFL PLGTRVYNRL MDFIKNQYWH RGYTEVITPN MYNMELWQTS GHADNYKDNM FTFNIEKQEF
401: GLKPMNCPGH CLIFQHRVRS YRELPMRLAD FGVLHRNEAS GALSGLTRVR RFQQDDAHIF CTTEQVKGEV QGVLEFIDYV YKVFGFTYEL KLSTRPEKYL
501: GDLETWDKAE ADLKEAIEAF GKPLVLNEGD GAFYGPKIDI TVSDAMNRKF QCATLQLDFQ LPIRFNLEYA AEDEAKKSRP VMIHRAVLGS VERMFAILLE
601: HYKGKWPFWI SPRQAIVCPI SEKSQQYAEK VQKQIKDAGF YVDADLTDRK IDKKVREAQL AQYNYILVVG ETEAATGQVS VRVRDNAAHS VKSIEDLLEE
701: FKAKTAEFV

Arabidopsis Description

THRRSThreonine--tRNA ligase, mitochondrial 1 [Source:UniProtKB/Swiss-Prot;Acc:O04630]
SUBAcon: [mitochondrion]

Hydropathy Plot

About CropPAL

The Protein Annotated Locations Database (CropPAL) houses large scale proteomic and GFP localization data from published experimental studies in Soybean (Glycine max), Maize (Zea mays), Wheat (Triticum aestivum), Barley (Hordeum vulgare), Rice (Oryza sativa), Field mustard (Brassica rapa), Canola (Brassica napus), Sorghum (Sorghum bicolor), Potato (Solanum tuberosum), Tomato (Solanum lycopersicum), Banana (Musa acuminata) and Wine grape (Vitis vinifera) as well as precomputed predictions for protein subcellular localizations using protein sequences.