CropPAL

Subcellular Localization

min:

: max

Winner_takes_all: nucleus

Predictor Summary:

plastid 7
cytosol 3
mitochondrion 1

Predictors

GFP

MS/MS

Papers

nucleus: 21132161

msms PMID: 21132161 doi

B Cooper, KB Campbell, J Feng, WM Garrett, R Frederick

Soybean Genomics and Improvement Laboratory, USDA-ARS, Beltsville, MD 20705, USA. bret.cooper@ars.usda.gov

PPI

Inferred distinct locusB in Crop

locusB	locations
KRG94268
KRH57705

Inferred from Arabidopsis experimental PPI

Ath locusA	locusB	Ath locusB	Paper
AT2G04842.1	KRG94268	AT5G40930.1	21848659
AT2G04842.1	KRH57705	AT5G40930.1	21848659

Homology

Paralog

locus	Identity	Homology Identity

Ortholog

locus	Homology Species	Location	Identity	Homology Identity
KRH17875	Soybean	mitochondrion	33.74	86.61
VIT_12s0057g00400.t01	Wine grape	plastid	79.91	76.51
Bra013193.1-P	Field mustard	plastid	76.07	76.07
CDX93065	Canola	plastid	76.38	75.57
AT2G04842.1	Thale cress	plastid	75.15	75.38
Solyc07g048080.2.1	Tomato	nucleus	77.45	75.04
CDY50043	Canola	plastid	75.0	75.0
GSMUA_Achr4P01570_001	Banana	plastid	76.84	75.0
PGSC0003DMT400015710	Potato	mitochondrion, plastid	77.15	74.85
KXG36582	Sorghum	plastid	74.85	73.38
TraesCS6D01G262800.1	Wheat	plastid	74.85	73.16
TraesCS6A01G282300.1	Wheat	plastid	74.23	72.56
TraesCS6B01G310700.1	Wheat	plastid	71.63	72.18
Os02t0538000-01	Rice	plasma membrane	74.54	72.11
HORVU6Hr1G070790.5	Barley	plastid	74.85	70.42
Zm00001d021962_P001	Maize	plastid	67.64	69.45
KRH69521	Soybean	endoplasmic reticulum, nucleus	35.74	32.82
KRH74628	Soybean	endoplasmic reticulum	35.89	32.64

Protein Annotations

KEGG:00970+6.1.1.3	EntrezGene:100784904	MapMan:17.2.19	Gene3D:3.30.54.20	Gene3D:3.30.930.10	Gene3D:3.30.980.10
Gene3D:3.40.50.800	EMBL:ACUP02012146	InterPro:Anticodon-bd	InterPro:Anticodon-bd_dom_sf	EnsemblPlantsGene:GLYMA_19G191200	GO:GO:0000166
GO:GO:0003674	GO:GO:0003824	GO:GO:0004812	GO:GO:0004829	GO:GO:0005488	GO:GO:0005524
GO:GO:0005575	GO:GO:0005622	GO:GO:0005623	GO:GO:0005737	GO:GO:0005739	GO:GO:0006139
GO:GO:0006412	GO:GO:0006418	GO:GO:0006435	GO:GO:0008150	GO:GO:0008152	GO:GO:0009058
GO:GO:0009536	GO:GO:0009570	GO:GO:0009987	GO:GO:0019538	GO:GO:0043039	UniProt:I1NAJ0
InterPro:IPR006195	InterPro:IPR036621	EnsemblPlants:KRG96128	ProteinID:KRG96128	ProteinID:KRG96128.1	HAMAP:MF_00184
PFAM:PF00587	PFAM:PF03129	PFAM:PF07973	PRINTS:PR01047	PFscan:PS50862	PANTHER:PTHR11451
PANTHER:PTHR11451:SF44	SMART:SM00863	SUPFAM:SSF52954	SUPFAM:SSF55186	SUPFAM:SSF55681	TIGRFAMs:TIGR00418
InterPro:Thr-tRNA-ligase_IIa	InterPro:Thr/Ala-tRNA-synth_IIc_edit	InterPro:ThrRS_core	UniParc:UPI000233F5EB	InterPro:aa-tRNA-synt_IIb	InterPro:aa-tRNA-synth_II
SEG:seg	InterPro:tRNA_SAD	:	:	:	:

Description

hypothetical protein

Coordinates

chr19:-:44859989..44865990

Molecular Weight (calculated)

74670.4 Da

IEP (calculated)

6.275

GRAVY (calculated)

-0.410

Length

652 amino acids

Sequence
(BLAST)

001: MATFAVATTL STSSIAPLNA RRGFGFGFGS FLSPTTKPKR PSITTTLAVA TDSSVCFNPN PNPNPNPNQL VLPSNESSEM LLRIRHTSAH VMAMAVQKLY
101: PDAKVTIGPW IENGFYYDFD MEPLTDKDLK RIKKEMDRII GKNLPLVREE VSRGEAHRRI SALNEPYKLE ILESIKDDPI TIYHIGDEWW DLCAGPHVDS
201: TGHINKKAVE LESIAGAYWR GDERKPMLQR IYGTAWENEE QLKAYLHFKE EAKRRDHRRL GQDLDLFSIQ DDAGGGLVFW HPKGAMVRHI IEDFWKKIHM
301: KRGYDLLYTP HVAKADLWKI SGHLDFYKEN MYDQMSVEDE LYQLRPMNCP YHILVYKSKL HSYRDFPIRV AELGTVYRYE LSGSLHGLFR VRGFTQDDAH
401: IFCLDDQIKD EIRGVLDLTE EILLQFGFEK YEVNLSTRPE KAVGDDDIWE KATSALEDAL DDKGWTYQID DGGGAFYGPK VDIKIEDALG RKWQCSTIQV
501: DFNLPQRFDI TYVDSNTEKR RPIMIHRAVL GSLERFFGIL IEHYAGDFPL WLSPIQARVL PVTDAQLEYC NVVTNKLKMY GIRAEVCHGE RLPKLIRNAE
601: KQKIPLMVVV GSKEVETETV TVRSRFGGEL GTMPVDDFID RIKLGTENPI SL

Best Arabidopsis Sequence Match ( AT2G04842.2 )
(BLAST)

001: MASSHSLLFS SSFLSKPSSF TSSLRRFVYL PTRQFWPRQR HGFSTVFAVA TEPAISSSGP KKAEPSTVVL PSNESSDKLL KIRHTCAHVM AMAVQKLFPD
101: AKVTIGPWID NGFYYDFDME PLTDKDLKRI KKEMDRIISR NLPLLREEVS REEAKKRIMA INEPYKMEIL DGIKEEPITV YHIGNEWWDL CAGPHVETTG
201: KINKKAVELE SVAGAYWRGD EKRQMLQRIY GTAWESEEQL KAYLHFKEEA KRRDHRRIGQ DLDLFSIQDE AGGGLVFWHP KGAIVRNIIE ESWKKMHVEH
301: GYDLIYTPHV AKADLWKISG HLDFYRENMY DQMEIEDELY QLRPMNCPYH ILLYQRKRQS YRDLPIRVAE LGTVYRYELS GSLHGLFRVR GFTQDDAHIF
401: CLEDQIKDEI RGVLDLTEEI LSRFGFNKYE VNLSTRPEKS VGGDDIWEKA TCALRDALDD KGWSYEVDEG GGAFYGPKID LKIEDALGRK WQCSTIQVDF
501: NLPQRFDITY VDTNSDKKRP IMIHRAVLGS LERFFGVLIE HYAGDFPLWL SPVQVRVLPV TDNQLEFCKE VSKKLRACGV RAELCHGERL PKLIRNAETQ
601: KIPLMAVVGP KEVETGTVTV RSRFGGELGT IPVDDLINKI NIAVETRTAL

Arabidopsis Description

EMB2761Threonine--tRNA ligase, chloroplastic/mitochondrial 2 [Source:UniProtKB/Swiss-Prot;Acc:F4IFC5]
SUBAcon: [plastid]

Hydropathy Plot

About CropPAL

The Protein Annotated Locations Database (CropPAL) houses large scale proteomic and GFP localization data from published experimental studies in Soybean (Glycine max), Maize (Zea mays), Wheat (Triticum aestivum), Barley (Hordeum vulgare), Rice (Oryza sativa), Field mustard (Brassica rapa), Canola (Brassica napus), Sorghum (Sorghum bicolor), Potato (Solanum tuberosum), Tomato (Solanum lycopersicum), Banana (Musa acuminata) and Wine grape (Vitis vinifera) as well as precomputed predictions for protein subcellular localizations using protein sequences.