Factsheet for PGSC0003DMT400097200

Subcellular Localization

min:

: max

Winner_takes_all: mitochondrion, cytosol

Predictor Summary:

cytosol 4
mitochondrion 3

Predictors

GFP

MS/MS

Papers

mitochondrion: 24351685

msms PMID: 24351685 doi

F Salvato, JF Havelund, M Chen, RS Rao, A Rogowska-Wrzesinska, ON Jensen, DR Gang, JJ Thelen, IM Møller

Department of Biochemistry and Interdisciplinary Plant Group, University of Missouri, Columbia, Missouri 65211.

PPI

Inferred distinct locusB in Crop

locusB	locations
PGSC0003DMT400063187

Inferred from Arabidopsis experimental PPI

Ath locusA	locusB	Ath locusB	Paper
AT5G26830.1	PGSC0003DMT400063187	AT3G48100.1	18642946

Homology

Paralog

locus	Identity	Homology Identity

Ortholog

locus	Homology Species	Location	Identity	Homology Identity
CDY21726	Canola	cytosol	56.42	53.44
CDX81854	Canola	cytosol	55.93	53.35
Bra031009.1-P	Field mustard	cytosol	47.7	52.81
AT1G18130.1	Thale cress	cytosol	20.82	52.12
Solyc08g074550.2.1	Tomato	plastid	78.69	48.08
Solyc05g005800.2.1	Tomato	plastid	78.93	44.97
TraesCS1B01G248400.1	Wheat	cytosol	62.95	44.07
CDY19754	Canola	cytosol	64.41	43.68
VIT_19s0015g00470.t01	Wine grape	cytosol	71.91	43.29
VIT_01s0026g00340.t01	Wine grape	cytosol	71.91	43.29
TraesCS1D01G236900.1	Wheat	cytosol, mitochondrion	59.81	40.56
TraesCS3B01G080400.1	Wheat	golgi, unclear	67.07	39.24
KRH74628	Soybean	endoplasmic reticulum	68.04	39.19
KRH69521	Soybean	endoplasmic reticulum, nucleus	67.31	39.15
TraesCS3A01G067300.1	Wheat	mitochondrion	67.07	39.12
Zm00001d027762_P001	Maize	cytosol	20.58	38.99
TraesCS3D01G066900.1	Wheat	mitochondrion	66.83	38.98
TraesCS1A01G235200.1	Wheat	cytosol	64.41	38.78
CDY21715	Canola	cytosol	58.84	38.69
Bra036596.1-P	Field mustard	mitochondrion	65.86	38.47
HORVU3Hr1G012410.6	Barley	mitochondrion	67.31	38.29
Zm00001d032667_P005	Maize	mitochondrion	68.28	38.26
EER91117	Sorghum	mitochondrion	68.04	38.02
CDX87030	Canola	mitochondrion, plastid	66.1	37.97
CDX80596	Canola	extracellular	65.62	37.96
AT5G26830.1	Thale cress	mitochondrion	65.13	37.94
Os08t0295300-01	Rice	golgi, plasma membrane	68.28	37.85
CDX88245	Canola	mitochondrion	65.62	37.74
Bra009922.1-P	Field mustard	mitochondrion	65.62	37.74
Bra031021.1-P	Field mustard	cytosol	52.3	37.44
AT1G17960.1	Thale cress	cytosol	41.4	37.34
Zm00001d013607_P003	Maize	mitochondrion	67.55	34.11
HORVU1Hr1G057910.4	Barley	cytosol	29.3	30.4
PGSC0003DMT400015710	Potato	mitochondrion, plastid	39.95	24.55
PGSC0003DMT400076047	Potato	cytosol	0.0	0.0

Protein Annotations

KEGG:00970+6.1.1.3	EntrezGene:102605285	MapMan:17.2.19	Gene3D:3.30.930.10	Gene3D:3.40.50.800	InterPro:Anticodon-bd
InterPro:Anticodon-bd_dom_sf	GO:GO:0000166	GO:GO:0003674	GO:GO:0003824	GO:GO:0004812	GO:GO:0004829
GO:GO:0005488	GO:GO:0005524	GO:GO:0005575	GO:GO:0005622	GO:GO:0005623	GO:GO:0005737
GO:GO:0006139	GO:GO:0006412	GO:GO:0006418	GO:GO:0006435	GO:GO:0008150	GO:GO:0008152
GO:GO:0009058	GO:GO:0009987	GO:GO:0019538	InterPro:IPR006195	InterPro:IPR036621	UniProt:M1E043
PFAM:PF00587	PFAM:PF03129	EnsemblPlantsGene:PGSC0003DMG400046771	PGSC:PGSC0003DMG400046771	EnsemblPlants:PGSC0003DMT400097200	PRINTS:PR01047
PFscan:PS50862	PANTHER:PTHR11451	SUPFAM:SSF52954	SUPFAM:SSF55681	TIGRFAMs:TIGR00418	InterPro:Thr-tRNA-ligase_IIa
InterPro:ThrRS_core	UniParc:UPI000294A042	RefSeq:XP_006346202.1	InterPro:aa-tRNA-synt_IIb	InterPro:aa-tRNA-synth_II	:

Description

Threonyl-tRNA synthetase [Source:PGSC_GENE;Acc:PGSC0003DMG400046771]

Coordinates

chr7:+:54962426..54963667

Molecular Weight (calculated)

48322.7 Da

IEP (calculated)

6.988

GRAVY (calculated)

-0.505

Length

413 amino acids

Sequence
(BLAST)

001: MGQENVETLE EANKYDHREL NKKQELFYFD PMSPGSCFFL PHGARICNKL LEFLKNEYWK RGYEEIWSPD IYKMQLWETS GHAAKYKENM FVFEIDDKAV
101: DQFGLKPMNC PGHCKVFGHR VRSYRELPLR LADFGALHRN EASGALTGLT RLRQFHQDDA HIFCRESQIK DEVKGVLDFI SYVYTIFGFS NVDFKLSTRP
201: EKYIGNLQSW KKAEDALAEA LNEFGKPWEI NKGDGAFYGP KIDISVSDAM KRNLQCATLQ LDFQLPERFN LLYVAEYDQI RERPVMIHRA ILGSVERMFA
301: ILFEHYKGKW PFWLSPRQAI VCPVSNKSQS YAFEIWKRLH DARYHVDIDV SDKTVGKKVR EAQLVQYNYI LVVGEEEAKN GQVSVRVRDK RDHQVMTVDE
401: LLDQFKDMVA SFQ

Best Arabidopsis Sequence Match ( AT5G26830.1 )
(BLAST)

001: MLLRLTARSI RRFTTSSSSL PLLSSSSFCT VPTMAANHPK DEAYLSAVIP KRIKLFEQIQ ANQLENLKSL PHDPIKVTLP DGNVKEGKKW ETTPMDIAAQ
101: ISKGLANSAL ISAVDDVLWD MNRPLEGDCK LELFKFDSDK GRDTLWHSSA HILGQALEQE YGCQLCIGPC TTRGEGFYYD GFYGELGLSD NHFPSIEAGA
201: AKAAKEAQPF ERIEVTKDQA LEMFSENNFK VELINGLPAD MTITVYRCGP LVDLCRGPHI PNTSFVKAFK CLRASSAYWK GDKDRESLQR VYGISYPDQK
301: QLKKYLQFLE EAKKYDHRLL GQKQELFFSH QLSPGSYFFL PLGTRVYNRL MDFIKNQYWH RGYTEVITPN MYNMELWQTS GHADNYKDNM FTFNIEKQEF
401: GLKPMNCPGH CLIFQHRVRS YRELPMRLAD FGVLHRNEAS GALSGLTRVR RFQQDDAHIF CTTEQVKGEV QGVLEFIDYV YKVFGFTYEL KLSTRPEKYL
501: GDLETWDKAE ADLKEAIEAF GKPLVLNEGD GAFYGPKIDI TVSDAMNRKF QCATLQLDFQ LPIRFNLEYA AEDEAKKSRP VMIHRAVLGS VERMFAILLE
601: HYKGKWPFWI SPRQAIVCPI SEKSQQYAEK VQKQIKDAGF YVDADLTDRK IDKKVREAQL AQYNYILVVG ETEAATGQVS VRVRDNAAHS VKSIEDLLEE
701: FKAKTAEFV

Arabidopsis Description

THRRSThreonine--tRNA ligase, mitochondrial 1 [Source:UniProtKB/Swiss-Prot;Acc:O04630]
SUBAcon: [mitochondrion]

Hydropathy Plot

About CropPAL

The Protein Annotated Locations Database (CropPAL) houses large scale proteomic and GFP localization data from published experimental studies in Soybean (Glycine max), Maize (Zea mays), Wheat (Triticum aestivum), Barley (Hordeum vulgare), Rice (Oryza sativa), Field mustard (Brassica rapa), Canola (Brassica napus), Sorghum (Sorghum bicolor), Potato (Solanum tuberosum), Tomato (Solanum lycopersicum), Banana (Musa acuminata) and Wine grape (Vitis vinifera) as well as precomputed predictions for protein subcellular localizations using protein sequences.