Factsheet for Zm00001d048531_P001

Subcellular Localization

min:

: max

Winner_takes_all: plastid

Predictor Summary:

plastid 2
mitochondrion 3
cytosol 1
nucleus 1

Predictors

GFP

MS/MS

Papers

plastid: 22065420
plastid: 23198870
mitochondrion: 27297264
plasma membrane: 27341663
mitochondrion: 29575040

msms PMID: 27297264 doi

D Dahal, KJ Newton, BP Mooney

Division of Biological Sciences, ‡Department of Biochemistry, and §The Charles W Gehrke Proteomics Center, University of Missouri , Columbia, Missouri 65211, United States.

msms PMID: 23198870 doi

M Huang, G Friso, K Nishimura, X Qu, PD Olinares, W Majeran, Q Sun, KJ van Wijk

Department of Plant Biology, Cornell University, Ithaca, New York 14853, United States.

msms PMID: 27341663 doi

P Voothuluru, JC Anderson, RE Sharp, SC Peck

Bond Life Sciences Center, University of Missouri, Columbia, MO, 65211, USA., Division of Biochemistry, University of Missouri, Columbia, MO, 65211, USA., Division of Plant Sciences, University of Missouri, Columbia, MO, 65211, USA., Interdisciplinary Plant Group, University of Missouri, Columbia, MO, 65211, USA.

msms PMID: 29575040 doi

WQ Wang, Y Wang, Q Zhang, IM Møller, SQ Song

Department of Molecular Biology and Genetics, Aarhus University, DK-4200 Slagelse, Denmark., Key Laboratory of Plant Molecular Physiology, Institute of Botany, the Chinese Academy of Sciences, Beijing 100093, China., Key Laboratory of Plant Resources and Beijing Botanical Garden, Institute of Botany, the Chinese Academy of Sciences, Beijing 100093, China.

msms PMID: 22065420 doi

W Majeran, G Friso, Y Asakura, X Qu, M Huang, L Ponnala, KP Watkins, A Barkan, KJ van Wijk

Department of Plant Biology, Cornell University, Ithaca, New York 14853, USA.

PPI

Inferred distinct locusB in Crop

locusB	locations
Zm00001d000402_P001
Zm00001d003996_P001
Zm00001d006417_P002
Zm00001d021532_P001
Zm00001d025208_P002

Inferred from Arabidopsis experimental PPI

Ath locusA	locusB	Ath locusB	Paper
AT3G06980.1	Zm00001d006417_P002	AT1G30860.1	21798944
AT3G06980.1	Zm00001d021532_P001	AT1G30860.1	21798944
AT3G06980.1	Zm00001d000402_P001	AT3G11590.1	21798944
AT3G06980.1	Zm00001d003996_P001	AT3G11590.1	21798944
AT3G06980.1	Zm00001d025208_P002	AT3G11590.1	21798944
AT3G06980.1	Zm00001d000402_P001	AT5G22310.1	21798944
AT3G06980.1	Zm00001d003996_P001	AT5G22310.1	21798944
AT3G06980.1	Zm00001d025208_P002	AT5G22310.1	21798944

Homology

Paralog

locus	Identity	Homology Identity

Ortholog

locus	Homology Species	Location	Identity	Homology Identity
EER95670	Sorghum	mitochondrion, plastid	92.67	92.53
Os03t0108600-01	Rice	mitochondrion, plastid	79.54	81.28
TraesCS3B01G568500.1	Wheat	mitochondrion	70.23	71.32
TraesCS5A01G548800.2	Wheat	mitochondrion, plastid	69.92	69.6
Solyc11g045180.1.1	Tomato	nucleus	19.08	69.06
TraesCS4D01G359000.1	Wheat	plastid	69.62	68.99
HORVU4Hr1G090840.3	Barley	plastid	70.23	68.86
KRH51370	Soybean	nucleus	46.26	57.17
KRH60674	Soybean	nucleus	45.04	55.87
CDX73166	Canola	cytosol	15.42	52.6
GSMUA_Achr3P24670_001	Banana	plastid	52.37	51.42
VIT_05s0020g01130.t01	Wine grape	mitochondrion	49.47	45.83
Bra029613.1-P	Field mustard	plastid	49.47	43.55
AT3G06980.1	Thale cress	plastid	48.85	40.97
Zm00001d020357_P003	Maize	plastid	18.78	21.43

Protein Annotations

EntrezGene:100280046	Gene3D:3.40.50.300	MapMan:35.1	ProteinID:AQL10140.1	UniProt:B8A1K2	EMBL:BT055444
InterPro:DEAD/DEAH_box_helicase_dom	GO:GO:0000166	GO:GO:0003674	GO:GO:0003676	GO:GO:0003724	GO:GO:0003824
GO:GO:0004004	GO:GO:0004386	GO:GO:0005488	GO:GO:0005524	GO:GO:0005575	GO:GO:0005622
GO:GO:0005623	GO:GO:0005737	GO:GO:0006950	GO:GO:0006968	GO:GO:0008150	GO:GO:0009507
GO:GO:0009536	GO:GO:0009605	GO:GO:0009607	GO:GO:0009719	GO:GO:0009817	GO:GO:0009987
GO:GO:0016787	GO:GO:0071369	GO:GO:0071395	GO:GO:0071446	InterPro:Helicase_ATP-bd	InterPro:Helicase_C
InterPro:IPR001650	InterPro:IPR014001	InterPro:IPR014014	InterPro:P-loop_NTPase	PFAM:PF00270	PFAM:PF00271
PFscan:PS51192	PFscan:PS51194	PFscan:PS51195	PANTHER:PTHR24031	PANTHER:PTHR24031:SF181	InterPro:RNA_helicase_DEAD_Q_motif
SMART:SM00487	SMART:SM00490	SUPFAM:SSF52540	UniParc:UPI0001917696	EnsemblPlantsGene:Zm00001d048531	EnsemblPlants:Zm00001d048531_P001
EnsemblPlants:Zm00001d048531_T001	SEG:seg	:	:	:	:

Description

DEAD-box ATP-dependent RNA helicase 50

Coordinates

chr9:-:157939097..157942646

Molecular Weight (calculated)

72465.8 Da

IEP (calculated)

8.757

GRAVY (calculated)

-0.458

Length

655 amino acids

Sequence
(BLAST)

001: MMEVAGAQAR AVPLLLRNTA SLRTSVSVSC TGSRRSWVAA ATAEGDETRG FDKVPMDTPG AYRLVDRDTG RSVIVWGGTD DSDEASMPSP AVLSRTTDRR
101: HSQGVGGGTG IGNFGRFKAQ KIKSLVTRSA HRKRESSNRS STAWSDESSF NGSDDEEESY FERRKHVSDS ERHPKMSSGS RDGRTRSAHS LSSVLSQYRG
201: DDDTDFPGSE ATSGSKRWGN VADVTYGRQN QKQREPLNFP QRKGPLDGGF FSRRSFKEIG CSDEMLGALR NFDFPRPSHI QAMAYGPILE GRSCVVADQS
301: GSGKTLAYLC PIIQNLRNEE VQGLHKSSPR NPRVIVLTPT AELASQVLNN CRLISKSGVP FRSMVATGGF RQKTQLESLD QELDVIIATP GRFLYLLQEG
401: FVQLANLRCV VLDEVDILFG EEGFEQVLHQ LITVAPVTTQ YLFVTATLPL DIYNKVVETF PDCEVIMGPG VHRTSSRLEE ILVDCSGDDN EEKNPETAFS
501: NKKTALLKII EESPVRKTIV FCNKIETCRK VENALRRVDR KASQIKVLPF HAALDQAQRI ANIKEFLNKQ TADSMFLVCT DRASRGIDFA NVNHVVLFDY
601: PRDPSEYVRR VGRTARGASG NGKAFVFAVG KQVSLARRVM ERNMKGHPLH DVPCV

Best Arabidopsis Sequence Match ( AT3G06980.1 )
(BLAST)

001: MLARAPPPYF NFPARNNTIC NRNEIVRLFR NGGGVVARGA GFTRRPLETS SSYDDSTDDG FVIISAADKE NEFAPPPSSD LLSSIPSESA RRNGSRSRGL
101: TASFGRLKAQ KVKALVGKVT QKKQHMSHNE EEDEDDASDE NYSADEGFGS SSILDLMRKK LAMKAIPRSG KSAERNEVKR ASKVRESRES RRDLDRLEGD
201: DEDVDEVSNP DRFTDNQRAG SRSSYSKGGY AANSRGKGDR LSVARDLDSF EGHGRAIDEV SNPRKFNDNE RAESRSSYSR DSSANSRGRE DRRFVAKELD
301: TFQGRDKAYD EVYNPRRFTD NERGLRGGSH SKGSDTNSRG WGDRRSVVYT RDMDDWRERN KTKDTRETGF FSRKTFAEIG CSEDMMKALK EQNFDRPAHI
401: QAMAFSPVID GKSCIIADQS GSGKTLAYLV PVIQRLREEE LQGHSKSSPG CPRVIVLVPT AELASQVLAN CRSISKSGVP FRSMVVTGGF RQRTQLENLE
501: QGVDVLIATP GRFTYLMNEG ILGLSNLRCA ILDEVDILFG DDEFEAALQN LINSSPVTAQ YLFVTATLPL EIYNKLVEVF PDCEVVMGPR VHRVSNALEE
601: FLVDCSGDDN AEKTPETAFQ NKKTALLQIM EENPVSKTII FCNKIETCRK VENIFKRVDR KERQLHVLPF HAALSQESRL TNMQEFTSSQ PEENSLFLVC
701: TDRASRGIDF SGVDHVVLFD FPRDPSEYVR RVGRTARGAR GKGKAFIFVV GKQVGLARRI IERNEKGHPV HDVPNAYEFT T

Arabidopsis Description

RH50DEAD-box ATP-dependent RNA helicase 50 [Source:UniProtKB/Swiss-Prot;Acc:Q8GUG7]
SUBAcon: [plastid]

Hydropathy Plot

About CropPAL

The Protein Annotated Locations Database (CropPAL) houses large scale proteomic and GFP localization data from published experimental studies in Soybean (Glycine max), Maize (Zea mays), Wheat (Triticum aestivum), Barley (Hordeum vulgare), Rice (Oryza sativa), Field mustard (Brassica rapa), Canola (Brassica napus), Sorghum (Sorghum bicolor), Potato (Solanum tuberosum), Tomato (Solanum lycopersicum), Banana (Musa acuminata) and Wine grape (Vitis vinifera) as well as precomputed predictions for protein subcellular localizations using protein sequences.