CropPAL

Subcellular Localization

min:

: max

Winner_takes_all: nucleus

Predictor Summary:

plastid 8
nucleus 2

Predictors

GFP

MS/MS

Papers

nucleus: 21132161

msms PMID: 21132161 doi

B Cooper, KB Campbell, J Feng, WM Garrett, R Frederick

Soybean Genomics and Improvement Laboratory, USDA-ARS, Beltsville, MD 20705, USA. bret.cooper@ars.usda.gov

PPI

Inferred distinct locusB in Crop

locusB	locations
KRG96225
KRH12155
KRH25284
KRH30384
KRH37161
KRH67971

Inferred from Arabidopsis experimental PPI

Ath locusA	locusB	Ath locusB	Paper
AT3G06980.1	KRH12155	AT1G30860.1	21798944
AT3G06980.1	KRH37161	AT1G30860.1	21798944
AT3G06980.1	KRG96225	AT3G11590.1	21798944
AT3G06980.1	KRH67971	AT3G11590.1	21798944
AT3G06980.1	KRH25284	AT5G22310.1	21798944
AT3G06980.1	KRH30384	AT5G22310.1	21798944

Homology

Paralog

locus	Identity	Homology Identity

Ortholog

locus	Homology Species	Location	Identity	Homology Identity
KRH51370	Soybean	nucleus	76.14	75.85
CDY24656	Canola	mitochondrion	58.52	48.51
CDY05302	Canola	plastid	57.01	46.81
VIT_05s0020g01130.t01	Wine grape	mitochondrion	61.93	46.25
Os03t0108600-01	Rice	mitochondrion, plastid	55.11	45.4
Zm00001d048531_P001	Maize	plastid	55.87	45.04
TraesCS3B01G568500.1	Wheat	mitochondrion	54.73	44.81
EER95670	Sorghum	mitochondrion, plastid	55.49	44.66
TraesCS5A01G548800.2	Wheat	mitochondrion, plastid	54.73	43.92
TraesCS4D01G359000.1	Wheat	plastid	54.73	43.72
HORVU4Hr1G090840.3	Barley	plastid	54.73	43.26
GSMUA_Achr3P24670_001	Banana	plastid	53.22	42.13
Bra029613.1-P	Field mustard	plastid	57.39	40.73
AT3G06980.1	Thale cress	plastid	58.71	39.69
Solyc11g045190.1.1	Tomato	nucleus	32.39	34.76
KRH70245	Soybean	nucleus	26.14	21.7
KRG99241	Soybean	plastid	22.73	20.73
KRH08609	Soybean	plastid	14.02	19.84
KRH70239	Soybean	mitochondrion	12.31	17.43

Protein Annotations

EntrezGene:100784377	Gene3D:3.40.50.300	MapMan:35.1	EMBL:ACUP02002135	InterPro:DEAD/DEAH_box_helicase_dom	EnsemblPlantsGene:GLYMA_04G002600
GO:GO:0000166	GO:GO:0003674	GO:GO:0003676	GO:GO:0003824	GO:GO:0004004	GO:GO:0005488
GO:GO:0005524	GO:GO:0005575	GO:GO:0005622	GO:GO:0005623	GO:GO:0005634	GO:GO:0005730
GO:GO:0005737	GO:GO:0006139	GO:GO:0008150	GO:GO:0008152	GO:GO:0009987	GO:GO:0010501
GO:GO:0016787	InterPro:Helicase_ATP-bd	InterPro:Helicase_C	UniProt:I1JSC9	InterPro:IPR001650	InterPro:IPR014001
InterPro:IPR014014	EnsemblPlants:KRH60674	ProteinID:KRH60674	ProteinID:KRH60674.1	InterPro:P-loop_NTPase	PFAM:PF00270
PFAM:PF00271	PFscan:PS51192	PFscan:PS51194	PFscan:PS51195	PANTHER:PTHR24031	PANTHER:PTHR24031:SF181
InterPro:RNA_helicase_DEAD_Q_motif	SMART:SM00487	SMART:SM00490	SUPFAM:SSF52540	UniParc:UPI0002339C1D	SEG:seg

Description

hypothetical protein

Coordinates

chr4:-:211968..215847

Molecular Weight (calculated)

58918.9 Da

IEP (calculated)

9.259

GRAVY (calculated)

-0.280

Length

528 amino acids

Sequence
(BLAST)

001: MAVGQLVPSN LFLNHSAATT LIPTSTPPRH RHLLCISSAN HTTATDNDSP FSSFGRVKTL LVHRRRKDQS HRRAVQLEDD NDDDIAPRPR RSQSRSRGGE
101: RWDMIPNYTP QSKSATDTKF FSLKSFKEIG CSEYMIESLQ KLLLSRPSHV QAMAFAPVIS GKTCVIADQS GSGKTLAYLA PIIQLLRLEE LEGRSSKSSS
201: QAPRVLVLAP TAELASQVLD NCRSLSKSGV PFKSMVVTGG FRQKTQLENL QQGVDVLIAT PGRFLFLIHE GFLQLTNLRC AILDEVDILF GDEDFEVALQ
301: SLINSSPVDT QYLFVTATLP KNVYTKLVEV FPDCEMIMGP GMHRISSRLQ EIIVDCSGED GQEKTPDTAF LNKKTALLQL VEENPVPRTI VFCNKIETCR
401: KVENLLKRFD RKGNHVQVLP FHAAMTQESR LASMEEFTRS PSKGVSQFMV CTDRASRGID FTRVDHVILF DFPRDPSEYV RRVGRTARGA KGVGKAFIFV
501: VGKQVSLARK IMERNQKGHP LHDVPSAY

Best Arabidopsis Sequence Match ( AT3G06980.1 )
(BLAST)

001: MLARAPPPYF NFPARNNTIC NRNEIVRLFR NGGGVVARGA GFTRRPLETS SSYDDSTDDG FVIISAADKE NEFAPPPSSD LLSSIPSESA RRNGSRSRGL
101: TASFGRLKAQ KVKALVGKVT QKKQHMSHNE EEDEDDASDE NYSADEGFGS SSILDLMRKK LAMKAIPRSG KSAERNEVKR ASKVRESRES RRDLDRLEGD
201: DEDVDEVSNP DRFTDNQRAG SRSSYSKGGY AANSRGKGDR LSVARDLDSF EGHGRAIDEV SNPRKFNDNE RAESRSSYSR DSSANSRGRE DRRFVAKELD
301: TFQGRDKAYD EVYNPRRFTD NERGLRGGSH SKGSDTNSRG WGDRRSVVYT RDMDDWRERN KTKDTRETGF FSRKTFAEIG CSEDMMKALK EQNFDRPAHI
401: QAMAFSPVID GKSCIIADQS GSGKTLAYLV PVIQRLREEE LQGHSKSSPG CPRVIVLVPT AELASQVLAN CRSISKSGVP FRSMVVTGGF RQRTQLENLE
501: QGVDVLIATP GRFTYLMNEG ILGLSNLRCA ILDEVDILFG DDEFEAALQN LINSSPVTAQ YLFVTATLPL EIYNKLVEVF PDCEVVMGPR VHRVSNALEE
601: FLVDCSGDDN AEKTPETAFQ NKKTALLQIM EENPVSKTII FCNKIETCRK VENIFKRVDR KERQLHVLPF HAALSQESRL TNMQEFTSSQ PEENSLFLVC
701: TDRASRGIDF SGVDHVVLFD FPRDPSEYVR RVGRTARGAR GKGKAFIFVV GKQVGLARRI IERNEKGHPV HDVPNAYEFT T

Arabidopsis Description

RH50DEAD-box ATP-dependent RNA helicase 50 [Source:UniProtKB/Swiss-Prot;Acc:Q8GUG7]
SUBAcon: [plastid]

Hydropathy Plot

About CropPAL

The Protein Annotated Locations Database (CropPAL) houses large scale proteomic and GFP localization data from published experimental studies in Soybean (Glycine max), Maize (Zea mays), Wheat (Triticum aestivum), Barley (Hordeum vulgare), Rice (Oryza sativa), Field mustard (Brassica rapa), Canola (Brassica napus), Sorghum (Sorghum bicolor), Potato (Solanum tuberosum), Tomato (Solanum lycopersicum), Banana (Musa acuminata) and Wine grape (Vitis vinifera) as well as precomputed predictions for protein subcellular localizations using protein sequences.