CropPAL

Subcellular Localization

min:

: max

Winner_takes_all: nucleus

Predictor Summary:

plastid 7
nucleus 1
mitochondrion 2

Predictors

GFP

MS/MS

Papers

nucleus: 21132161

msms PMID: 21132161 doi

B Cooper, KB Campbell, J Feng, WM Garrett, R Frederick

Soybean Genomics and Improvement Laboratory, USDA-ARS, Beltsville, MD 20705, USA. bret.cooper@ars.usda.gov

PPI

Inferred distinct locusB in Crop

locusB	locations
KRG96225
KRH12155
KRH25284
KRH30384
KRH37161
KRH67971

Inferred from Arabidopsis experimental PPI

Ath locusA	locusB	Ath locusB	Paper
AT3G06980.1	KRH12155	AT1G30860.1	21798944
AT3G06980.1	KRH37161	AT1G30860.1	21798944
AT3G06980.1	KRG96225	AT3G11590.1	21798944
AT3G06980.1	KRH67971	AT3G11590.1	21798944
AT3G06980.1	KRH25284	AT5G22310.1	21798944
AT3G06980.1	KRH30384	AT5G22310.1	21798944

Homology

Paralog

locus	Identity	Homology Identity

Ortholog

locus	Homology Species	Location	Identity	Homology Identity
KRH60674	Soybean	nucleus	75.85	76.14
CDY24656	Canola	mitochondrion	58.3	48.51
CDY05302	Canola	plastid	58.11	47.9
VIT_05s0020g01130.t01	Wine grape	mitochondrion	62.83	47.1
Os03t0108600-01	Rice	mitochondrion, plastid	56.23	46.49
Zm00001d048531_P001	Maize	plastid	57.17	46.26
EER95670	Sorghum	mitochondrion, plastid	56.6	45.73
TraesCS3B01G568500.1	Wheat	mitochondrion	55.47	45.58
TraesCS5A01G548800.2	Wheat	mitochondrion, plastid	55.09	44.38
TraesCS4D01G359000.1	Wheat	plastid	55.09	44.18
HORVU4Hr1G090840.3	Barley	plastid	55.09	43.71
GSMUA_Achr3P24670_001	Banana	plastid	54.91	43.63
Bra029613.1-P	Field mustard	plastid	58.3	41.53
AT3G06980.1	Thale cress	plastid	57.74	39.18
Solyc11g045190.1.1	Tomato	nucleus	33.02	35.57
KRG99241	Soybean	plastid	23.02	21.07
KRH70245	Soybean	nucleus	24.15	20.13
KRH08609	Soybean	plastid	13.4	19.03
KRH70239	Soybean	mitochondrion	12.45	17.69

Protein Annotations

EntrezGene:100780849	Gene3D:3.40.50.300	MapMan:35.1	UniProt:A0A0R0J9W7	EMBL:ACUP02003371	InterPro:DEAD/DEAH_box_helicase_dom
EnsemblPlantsGene:GLYMA_06G002600	GO:GO:0000166	GO:GO:0003674	GO:GO:0003676	GO:GO:0005488	GO:GO:0005524
InterPro:Helicase_ATP-bd	InterPro:Helicase_C	InterPro:IPR001650	InterPro:IPR014001	InterPro:IPR014014	EnsemblPlants:KRH51370
ProteinID:KRH51370	ProteinID:KRH51370.1	InterPro:P-loop_NTPase	PFAM:PF00270	PFAM:PF00271	PFscan:PS51192
PFscan:PS51194	PFscan:PS51195	PANTHER:PTHR24031	PANTHER:PTHR24031:SF181	InterPro:RNA_helicase_DEAD_Q_motif	SMART:SM00487
SMART:SM00490	SUPFAM:SSF52540	UniParc:UPI0001B83755	SEG:seg	:	:

Description

hypothetical protein

Coordinates

chr6:-:220584..224030

Molecular Weight (calculated)

59146.3 Da

IEP (calculated)

9.718

GRAVY (calculated)

-0.282

Length

530 amino acids

Sequence
(BLAST)

001: MVVGQLVPSN LFLNRSAATR HRHLLCVSAN DTTSTNNTDN GSPFSSFGRL KAQKVKTLLV HRRGKDQSHR RPVHLEDDDD YVATPRPRRS QSRTRGGERW
101: DMIPNYTPPA QSKSVSDTKF FSLKSFKEIG CSEYMIESLQ KLLFSRPSHV QAMAFTPVIS GKTCVIADQS GSGKTFAYLA PIIQRLRQQE LEGIISKSSS
201: QAPSPRVLVL APTAELASQV LDNCRSLSKS GVPFKSMVVT GGFRQKTQLE NLQQGVDVLI ATPGRFLFLI NQGFLHLTNL RCAVLDEVDI LFGDEDFEVA
301: LQSLINSSPV DTQYLFVTAT LPKNVYTKLV EVFPDCEMIM GPGMHRISSR LQEIIVDCSG EDGQEKTPDT AFLNKKTALL QLVEESPVPR TIVFCNKIET
401: CRKVENLLKR FDRKGNCVQV LPFHAAMTQE SRLASMEEFT RSPSKGVSQF MVCTDRASRG IDFARVDHVI LFDFPRDPSE YVRRVGRTAR GAKGVGKAFI
501: FVVGKQVSLA RKIMERNQKG HPLHDVPSAY

Best Arabidopsis Sequence Match ( AT3G06980.1 )
(BLAST)

001: MLARAPPPYF NFPARNNTIC NRNEIVRLFR NGGGVVARGA GFTRRPLETS SSYDDSTDDG FVIISAADKE NEFAPPPSSD LLSSIPSESA RRNGSRSRGL
101: TASFGRLKAQ KVKALVGKVT QKKQHMSHNE EEDEDDASDE NYSADEGFGS SSILDLMRKK LAMKAIPRSG KSAERNEVKR ASKVRESRES RRDLDRLEGD
201: DEDVDEVSNP DRFTDNQRAG SRSSYSKGGY AANSRGKGDR LSVARDLDSF EGHGRAIDEV SNPRKFNDNE RAESRSSYSR DSSANSRGRE DRRFVAKELD
301: TFQGRDKAYD EVYNPRRFTD NERGLRGGSH SKGSDTNSRG WGDRRSVVYT RDMDDWRERN KTKDTRETGF FSRKTFAEIG CSEDMMKALK EQNFDRPAHI
401: QAMAFSPVID GKSCIIADQS GSGKTLAYLV PVIQRLREEE LQGHSKSSPG CPRVIVLVPT AELASQVLAN CRSISKSGVP FRSMVVTGGF RQRTQLENLE
501: QGVDVLIATP GRFTYLMNEG ILGLSNLRCA ILDEVDILFG DDEFEAALQN LINSSPVTAQ YLFVTATLPL EIYNKLVEVF PDCEVVMGPR VHRVSNALEE
601: FLVDCSGDDN AEKTPETAFQ NKKTALLQIM EENPVSKTII FCNKIETCRK VENIFKRVDR KERQLHVLPF HAALSQESRL TNMQEFTSSQ PEENSLFLVC
701: TDRASRGIDF SGVDHVVLFD FPRDPSEYVR RVGRTARGAR GKGKAFIFVV GKQVGLARRI IERNEKGHPV HDVPNAYEFT T

Arabidopsis Description

RH50DEAD-box ATP-dependent RNA helicase 50 [Source:UniProtKB/Swiss-Prot;Acc:Q8GUG7]
SUBAcon: [plastid]

Hydropathy Plot

About CropPAL

The Protein Annotated Locations Database (CropPAL) houses large scale proteomic and GFP localization data from published experimental studies in Soybean (Glycine max), Maize (Zea mays), Wheat (Triticum aestivum), Barley (Hordeum vulgare), Rice (Oryza sativa), Field mustard (Brassica rapa), Canola (Brassica napus), Sorghum (Sorghum bicolor), Potato (Solanum tuberosum), Tomato (Solanum lycopersicum), Banana (Musa acuminata) and Wine grape (Vitis vinifera) as well as precomputed predictions for protein subcellular localizations using protein sequences.