CropPAL

Subcellular Localization

min:

: max

Winner_takes_all: endoplasmic reticulum

Predictor Summary:

extracellular 4
endoplasmic reticulum 7
vacuole 4
plasma membrane 4
golgi 4
plastid 1
nucleus 1

Predictors

GFP

MS/MS

Papers

Winner Takes All:	endoplasmic reticulum
Any Predictor:	nucleus, plastid, secretory
BaCelLo:	secretory
iPSORT:	plastid
MultiLoc:	endoplasmic reticulum
Plant-mPloc:	nucleus
Predotar:	secretory
PProwler:	secretory
TargetP:	secretory
WoLF PSORT:	endoplasmic reticulum
YLoc:	endoplasmic reticulum

nucleus: 21132161
endoplasmic reticulum: 27224218
endoplasmic reticulum: 28499913

msms PMID: 21132161 doi

B Cooper, KB Campbell, J Feng, WM Garrett, R Frederick

Soybean Genomics and Improvement Laboratory, USDA-ARS, Beltsville, MD 20705, USA. bret.cooper@ars.usda.gov

msms PMID: 28499913 doi

S Komatsu, X Wang, X Yin, Y Nanjo, H Ohyanagi, K Sakata

Department of Life Science and Informatics, Maebashi Institute of Technology, Maebashi 371-0816, Japan. Electronic address: ksakata@maebashi-it.ac.jp., Institute of Crop Science, National Agriculture and Food Research Organization, Tsukuba 305-8518, Japan., Institute of Crop Science, National Agriculture and Food Research Organization, Tsukuba 305-8518, Japan; Faculty of Life and Environmental Sciences, University of Tsukuba, Tsukuba 305-8572, Japan., Institute of Crop Science, National Agriculture and Food Research Organization, Tsukuba 305-8518, Japan; Faculty of Life and Environmental Sciences, University of Tsukuba, Tsukuba 305-8572, Japan. Electronic address: komatsu.setsuko.fu@u.tsukuba.ac.jp., King Abdullah University of Science and Technology (KAUST), Computational Bioscience Research Center (CBRC), Thuwal 23955-6900, Saudi Arabia.

msms PMID: 27224218 doi

X Wang, S Komatsu

Graduate School of Life and Environmental Sciences, University of Tsukuba , Tsukuba 305-8572, Japan., National Institute of Crop Science, National Agriculture and Food Research Organization , Tsukuba 305-8518, Japan.

PPI

Inferred distinct locusB in Crop

locusB	locations
KRH05183
KRH15859
KRH41720
KRH49658
KRH60419
KRH70354

Inferred from Arabidopsis experimental PPI

Ath locusA	locusB	Ath locusB	Paper
AT5G56360.1	KRH41720	AT1G68060.1	21798944
AT5G56360.1	KRH49658	AT1G68060.1	21798944
AT5G56360.1	KRH60419	AT1G68060.1	21798944
AT5G56360.1	KRH70354	AT1G68060.1	21798944
AT5G56360.1	KRH05183	AT4G32190.1	21798944
AT5G56360.1	KRH15859	AT4G32190.1	21798944

Homology

Paralog

locus	Identity	Homology Identity

Ortholog

locus	Homology Species	Location	Identity	Homology Identity
KRG89462	Soybean	endoplasmic reticulum	85.21	84.14
VIT_06s0004g00140.t01	Wine grape	endoplasmic reticulum	61.21	60.34
AT5G56360.1	Thale cress	endoplasmic reticulum	58.19	56.57
CDY32789	Canola	endoplasmic reticulum	56.44	55.38
PGSC0003DMT400011814	Potato	endoplasmic reticulum	55.33	55.06
Bra002821.1-P	Field mustard	endoplasmic reticulum	55.96	54.91
CDY46579	Canola	endoplasmic reticulum	56.12	54.81
CDY11931	Canola	endoplasmic reticulum	56.6	54.52
Bra028933.1-P	Field mustard	endoplasmic reticulum	55.96	54.07
TraesCS3B01G218600.2	Wheat	golgi	51.35	53.39
KXG32260	Sorghum	endoplasmic reticulum	51.83	53.27
TraesCS3D01G192900.1	Wheat	endoplasmic reticulum	51.19	53.22
CDY36796	Canola	endoplasmic reticulum	55.01	53.15
Solyc12g099660.1.1	Tomato	unclear	53.74	52.98
Os01t0276800-01	Rice	endoplasmic reticulum	51.51	52.77
TraesCS3A01G189400.3	Wheat	endoplasmic reticulum	50.72	52.73
HORVU3Hr1G039400.5	Barley	endoplasmic reticulum	51.19	52.27
GSMUA_Achr10P...	Banana	endoplasmic reticulum	22.58	51.45
Zm00001d040449_P001	Maize	endoplasmic reticulum	22.73	48.47
GSMUA_Achr5P27690_001	Banana	endoplasmic reticulum	49.44	45.67
Zm00001d008236_P004	Maize	endoplasmic reticulum	46.9	40.75
KRH55830	Soybean	plasma membrane	11.45	38.1
KRH25690	Soybean	plastid	11.45	31.86

Protein Annotations

EntrezGene:100807083	MapMan:18.1.1.6.2.2	Gene3D:2.70.130.10	EMBL:ACUP02004695	ncoils:Coil	EnsemblPlantsGene:GLYMA_07G224600
GO:GO:0006491	GO:GO:0008150	GO:GO:0008152	GO:GO:0009987	GO:GO:0019538	InterPro:Glucosidase_2_bsu
UniProt:I1KMB9	InterPro:IPR009011	EnsemblPlants:KRH50502	ProteinID:KRH50502	ProteinID:KRH50502.1	InterPro:Man6P_isomerase_rcpt-bd_dom_sf
PFAM:PF12999	PFAM:PF13015	InterPro:PRKCSH	InterPro:PRKCSH_N	PANTHER:PTHR12630	PANTHER:PTHR12630:SF10
SUPFAM:SSF50911	SignalP:SignalP-noTM	UniParc:UPI00023C5959	SEG:seg	:	:

Description

hypothetical protein

Coordinates

chr7:+:40165977..40177352

Molecular Weight (calculated)

71173.9 Da

IEP (calculated)

4.354

GRAVY (calculated)

-1.148

Length

629 amino acids

Sequence
(BLAST)

001: MKLLIALLLV LCAPFSSSSK PKDPFLGISP EDDKYYKASD VIRCKDGSGK FTKAQLNDDF CDCADGTDEP GTSACPGGKF YCRNAGHSPV YLFSSRVNDG
101: ICDCCDGTDE YDGQVKCPNT CWEAGKVARD RLEKKIATYQ EGVKLRKLEM EQAKVAMEKD EAELSKLKKE ESILKGIVKQ LKDHKEQIEK AEEKERLQKE
201: KEEKQKKESE EKANEAKDKA DEDTGHRNEA EKHSDVEDNS VENNHDKIEN LEGSPADQDE AGDKLEDVLD NDDEASDSPG SEGSLHNKVE ENAKEAEEEP
301: IVKSETDIKV GNKESSAEII NKGNDASENT EGLSREELGR LVASRWTGEN TDKSSAEPDT TLDNEDHEDL PKGTNNEEYE GYASETDDDI DSNKYDDDSH
401: KYDDEDEVDE EYREDEHDDL TSSYKSDSDN EPDLSDNPSW LEKIQRTVRN IFQAVNLFQA PVNQSDAARV RKEYDESSAK LSKIQSRISS LKQKLKHDFG
501: PAKEFYSFYD HCFEGKENKY TYKVCPYKQA SQEEGYSNTR LGSWDKFEDS YRVMVFSNGD KCWNGPDRSL KVKLRCGLKN EITDVDEPSR CEYVAVLSTP
601: ALCQEERLKE LQHKLDLLNS EIPSNHDEL

Best Arabidopsis Sequence Match ( AT5G56360.1 )
(BLAST)

001: MRVVVISSFV SVSLQLSFLL LLASAIRSSS SPPNDPFLGI SPQDEKYYKS SSEIKCKDGS KKFTKAQLND DFCDCSDGTD EPGTSACPTG KFYCRNAGHS
101: PVILFSSRVN DGICDCCDGS DEYDGHVSCQ NTCWEAGKAA RENLKKKIET YNQGLVIRRQ EIEQAKVGLE KDAAELKKLK SEQKILKGLV DQLKDRKEQI
201: EKVEEKERLQ KEKEEKEKKE AELAAQQGKG DAEEKTDDSE KVEESSHDEG TPAVSQHDET THHDEIGNYK DYPSDEEPAA EGEPTSILDE ATHTNPADEH
301: VVERKEESTS SEDSSSPTDE SQNDGSAEKE ESDEVKKVED FVTEKKEELS KEELGRLVAS RWTGEKSDKP TEADDIPKAD DQENHEHTPI TAHEADEDDG
401: FVSDGDEDTS DDGKYSDHEP EDDSYEEEYR HDSSSSYKSD ADDDVDFSET TSNPTWLEKI QKTVKNILLA VNLFQTTPVD KSEADRVRKE YDESSSKLNK
501: IQSRISSLEK KLKQDFGPEK EFYSFHGRCF ESKQGKYTYK VCAYKEATQE EGYSKTRLGE WDKFENSYQF MSYTNGEKCW NGPDRSLKVK LRCGLKNELM
601: DVDEPSRCEY AAILSTPARC LEDKLKELQQ KLEKLMNQDK PQNHDEL

Arabidopsis Description

PSL4Glucosidase 2 subunit beta [Source:UniProtKB/Swiss-Prot;Acc:Q9FM96]
SUBAcon: [endoplasmic reticulum]

Hydropathy Plot

About CropPAL

The Protein Annotated Locations Database (CropPAL) houses large scale proteomic and GFP localization data from published experimental studies in Soybean (Glycine max), Maize (Zea mays), Wheat (Triticum aestivum), Barley (Hordeum vulgare), Rice (Oryza sativa), Field mustard (Brassica rapa), Canola (Brassica napus), Sorghum (Sorghum bicolor), Potato (Solanum tuberosum), Tomato (Solanum lycopersicum), Banana (Musa acuminata) and Wine grape (Vitis vinifera) as well as precomputed predictions for protein subcellular localizations using protein sequences.