Factsheet for Solyc12g099660.1.1

Subcellular Localization

min:

: max

Winner_takes_all: unclear

Predictor Summary:

nucleus 2
cytosol 1
extracellular 4
endoplasmic reticulum 6
vacuole 5
plasma membrane 4
golgi 4

Predictors

GFP

MS/MS

Papers

Winner Takes All:	unclear
Any Predictor:	cytosol, nucleus, secretory
BaCelLo:	nucleus
EpiLoc:	cytosol
iPSORT:	secretory
MultiLoc:	endoplasmic reticulum
Plant-mPloc:	nucleus
Predotar:	secretory
PProwler:	secretory
TargetP:	secretory
WoLF PSORT:	vacuole
YLoc:	endoplasmic reticulum

unclear: 26455813

msms PMID: 26455813 doi

P Paul, P Chaturvedi, M Selymesi, A Ghatak, A Mesihovic, KD Scharf, W Weckwerth, S Simm, E Schleiff

Goethe University, Department of Biosciences, Molecular Cell Biology of Plants, Frankfurt/Main, Germany., Goethe University, Department of Biosciences, Molecular Cell Biology of Plants, Frankfurt/Main, Germany; SPOT-ITN Consortium., Goethe University, Department of Biosciences, Molecular Cell Biology of Plants, Frankfurt/Main, Germany; SPOT-ITN Consortium; Goethe University, Cluster of Excellence Frankfurt, Frankfurt/Main, Germany; Goethe University, Buchmann Institute of Molecular Life Sciences, Frankfurt/Main, Germany. Electronic address: schleiff@bio.uni-frankfurt.de., SPOT-ITN Consortium; University of Vienna, Department of Ecogenomics and Systems biology, Vienna, Austria., University of Vienna, Department of Ecogenomics and Systems biology, Vienna, Austria; School of Biotechnology and Bioinformatics, D.Y. Patil University, Mumbai, India.

PPI

Inferred distinct locusB in Crop

locusB	locations
Solyc01g005330.2.1
Solyc04g008040.2.1
Solyc05g017980.2.1
Solyc08g022030.2.1

Inferred from Arabidopsis experimental PPI

Ath locusA	locusB	Ath locusB	Paper
AT5G56360.1	Solyc01g005330.2.1	AT1G68060.1	21798944
AT5G56360.1	Solyc04g008040.2.1	AT1G68060.1	21798944
AT5G56360.1	Solyc05g017980.2.1	AT1G68060.1	21798944
AT5G56360.1	Solyc08g022030.2.1	AT4G32190.1	21798944

Homology

Paralog

locus	Identity	Homology Identity

Ortholog

locus	Homology Species	Location	Identity	Homology Identity
PGSC0003DMT400011814	Potato	endoplasmic reticulum	86.05	86.87
VIT_06s0004g00140.t01	Wine grape	endoplasmic reticulum	56.11	56.11
KRH50502	Soybean	endoplasmic reticulum	52.98	53.74
GSMUA_Achr10P...	Banana	endoplasmic reticulum	23.2	53.62
AT5G56360.1	Thale cress	endoplasmic reticulum	54.08	53.32
CDY32789	Canola	endoplasmic reticulum	53.13	52.89
KRG89462	Soybean	endoplasmic reticulum	52.66	52.75
CDY46579	Canola	endoplasmic reticulum	52.66	52.17
Bra002821.1-P	Field mustard	endoplasmic reticulum	51.88	51.64
CDY36796	Canola	endoplasmic reticulum	51.72	50.69
Bra028933.1-P	Field mustard	endoplasmic reticulum	51.57	50.54
CDY11931	Canola	endoplasmic reticulum	51.57	50.38
Os01t0276800-01	Rice	endoplasmic reticulum	45.45	47.23
KXG32260	Sorghum	endoplasmic reticulum	44.67	46.57
TraesCS3B01G218600.2	Wheat	golgi	43.42	45.79
TraesCS3D01G192900.1	Wheat	endoplasmic reticulum	43.42	45.79
HORVU3Hr1G039400.5	Barley	endoplasmic reticulum	44.04	45.62
TraesCS3A01G189400.3	Wheat	endoplasmic reticulum	43.1	45.45
Zm00001d040449_P001	Maize	endoplasmic reticulum	21.0	45.42
GSMUA_Achr5P27690_001	Banana	endoplasmic reticulum	46.71	43.76
Solyc10g008100.2.1	Tomato	plasma membrane	12.23	38.61
Zm00001d008236_P004	Maize	endoplasmic reticulum	39.81	35.08

Protein Annotations

MapMan:18.1.1.6.2.2	Gene3D:2.70.130.10	Gene3D:4.10.400.10	ncoils:Coil	GO:GO:0003674	GO:GO:0005488
GO:GO:0005515	GO:GO:0005575	GO:GO:0005622	GO:GO:0005623	GO:GO:0005737	GO:GO:0005783
GO:GO:0006491	GO:GO:0006950	GO:GO:0008150	GO:GO:0008152	GO:GO:0009605	GO:GO:0009607
GO:GO:0009987	GO:GO:0019538	GO:GO:0042742	InterPro:Glucosidase_2_bsu	InterPro:IPR009011	UniProt:K4DHY7
InterPro:LDL_receptor-like_sf	InterPro:LDrepeatLR_classA_rpt	InterPro:Man6P_isomerase_rcpt-bd_dom_sf	PFAM:PF12999	PFAM:PF13015	InterPro:PRKCSH
InterPro:PRKCSH_N	PANTHER:PTHR12630	PANTHER:PTHR12630:SF10	SUPFAM:SSF50911	SUPFAM:SSF57424	SignalP:SignalP-noTM
EnsemblPlantsGene:Solyc12g099660.1	EnsemblPlants:Solyc12g099660.1.1	UniParc:UPI000276A85A	SEG:seg	:	:

Description

Glucosidase 2 subunit beta [Source:Projected from Arabidopsis thaliana (AT5G56360) UniProtKB/Swiss-Prot;Acc:Q9FM96]

Coordinates

chr12:+:66713761..66722349

Molecular Weight (calculated)

72904.0 Da

IEP (calculated)

4.458

GRAVY (calculated)

-1.116

Length

638 amino acids

Sequence
(BLAST)

001: MELRFQFVFL LSCIFCISSI DRSVSLPSLV NLGIAPEDEN YYKGLSSGAI NCKDGSKKFT KAQLNDDFCD CPDGSDEPGT SACPSGKFYC KNAGHAPLFI
101: YSSRVNDGIC DCCDGSDEHD GKVKCPNTCW EVGRVARDKL KKKIATFQEG ITIRKKEIEE AKLAIAKEET EVSKLKNEQK ILKGRVEQLQ DKKEQIEKVE
201: EEERLKREKE EKERKEADDA KLEASKLEEK TEVHEEAVKS DIHDKIGLLE DYPPLKDVVE GHDKAADEEQ HDDHSVKDEF PVDEVEQAPE DSSQHPEVKE
301: ESSTNNNKAD VSSRNEEKDA AENIESLSKE ELGRVIGSRW LGKKSEQETV SVGAGTDSNH DNHDNHDEVP SDTHEEEYHG YDSDADDRKY DDDDEHKYDD
401: DEHKYDDDDN EEHVEDSVGE DHDSSSSYKS ESDDDSDFAD TTTTTSPSWT EKIQQTVKRI FRSVNLFQTP VNISDADRIR KEYDEASAKL TKVESRLSSL
501: KQKLKHDFGP EKEFYSFHGQ CFESKENKYT YKICPFKEAT QVEGYSTTRL GNWDKFEDSY RTMQFTNGDH CWNGPNRSLK VKLRCGLKNE VTDIDEPSRC
601: EYLAFFSTPA LCLEEKLKEL QDKLDMMNKE QPQDHDEL

Best Arabidopsis Sequence Match ( AT5G56360.1 )
(BLAST)

001: MRVVVISSFV SVSLQLSFLL LLASAIRSSS SPPNDPFLGI SPQDEKYYKS SSEIKCKDGS KKFTKAQLND DFCDCSDGTD EPGTSACPTG KFYCRNAGHS
101: PVILFSSRVN DGICDCCDGS DEYDGHVSCQ NTCWEAGKAA RENLKKKIET YNQGLVIRRQ EIEQAKVGLE KDAAELKKLK SEQKILKGLV DQLKDRKEQI
201: EKVEEKERLQ KEKEEKEKKE AELAAQQGKG DAEEKTDDSE KVEESSHDEG TPAVSQHDET THHDEIGNYK DYPSDEEPAA EGEPTSILDE ATHTNPADEH
301: VVERKEESTS SEDSSSPTDE SQNDGSAEKE ESDEVKKVED FVTEKKEELS KEELGRLVAS RWTGEKSDKP TEADDIPKAD DQENHEHTPI TAHEADEDDG
401: FVSDGDEDTS DDGKYSDHEP EDDSYEEEYR HDSSSSYKSD ADDDVDFSET TSNPTWLEKI QKTVKNILLA VNLFQTTPVD KSEADRVRKE YDESSSKLNK
501: IQSRISSLEK KLKQDFGPEK EFYSFHGRCF ESKQGKYTYK VCAYKEATQE EGYSKTRLGE WDKFENSYQF MSYTNGEKCW NGPDRSLKVK LRCGLKNELM
601: DVDEPSRCEY AAILSTPARC LEDKLKELQQ KLEKLMNQDK PQNHDEL

Arabidopsis Description

PSL4Glucosidase 2 subunit beta [Source:UniProtKB/Swiss-Prot;Acc:Q9FM96]
SUBAcon: [endoplasmic reticulum]

Hydropathy Plot

About CropPAL

The Protein Annotated Locations Database (CropPAL) houses large scale proteomic and GFP localization data from published experimental studies in Soybean (Glycine max), Maize (Zea mays), Wheat (Triticum aestivum), Barley (Hordeum vulgare), Rice (Oryza sativa), Field mustard (Brassica rapa), Canola (Brassica napus), Sorghum (Sorghum bicolor), Potato (Solanum tuberosum), Tomato (Solanum lycopersicum), Banana (Musa acuminata) and Wine grape (Vitis vinifera) as well as precomputed predictions for protein subcellular localizations using protein sequences.