CropPAL

Subcellular Localization

min:

: max

Winner_takes_all: nucleus, cytosol

Predictor Summary:

nucleus 1
cytosol 5
plastid 1

Predictors

GFP

MS/MS

Papers

Winner Takes All:	cytosol, nucleus
Any Predictor:	cytosol, nucleus, plastid
BaCelLo:	nucleus
EpiLoc:	cytosol
MultiLoc:	cytosol
Plant-mPloc:	cytosol
PProwler:	plastid
WoLF PSORT:	cytosol
YLoc:	cytosol

nucleus: 21132161

msms PMID: 21132161 doi

B Cooper, KB Campbell, J Feng, WM Garrett, R Frederick

Soybean Genomics and Improvement Laboratory, USDA-ARS, Beltsville, MD 20705, USA. bret.cooper@ars.usda.gov

PPI

Inferred distinct locusB in Crop

locusB	locations
KRH03094
KRH08461
KRH32861	plasma membrane
KRH54642
KRH56857
KRH63266
KRH70119
KRH72169	plasma membrane

Inferred from Arabidopsis experimental PPI

Ath locusA	locusB	Ath locusB	Paper
AT1G12360.1	KRH08461	AT1G08560.1	11157980
AT1G12360.1	KRH70119	AT1G08560.1	11157980
AT1G12360.1	KRH32861	AT3G11820.1	23572542
AT1G12360.1	KRH72169	AT3G11820.1	23572542
AT1G12360.1	KRH32861	AT3G11820.1	23572542
AT1G12360.1	KRH72169	AT3G11820.1	23572542
AT1G12360.1	KRH03094	AT5G07880.1	11591731
AT1G12360.1	KRH54642	AT5G07880.1	11591731
AT1G12360.1	KRH56857	AT5G07880.1	11591731
AT1G12360.1	KRH63266	AT5G07880.1	11591731
AT1G12360.1	KRH03094	AT5G61210.1	11591731
AT1G12360.1	KRH54642	AT5G61210.1	11591731
AT1G12360.1	KRH56857	AT5G61210.1	11591731
AT1G12360.1	KRH63266	AT5G61210.1	11591731

Homology

Paralog

locus	Identity	Homology Identity

Ortholog

locus	Homology Species	Location	Identity	Homology Identity
KRH04038	Soybean	cytosol	97.29	97.15
KRH28021	Soybean	nucleus	85.11	85.37
KRH77416	Soybean	cytosol	82.86	81.15
CDY26558	Canola	cytosol	19.25	80.5
VIT_13s0019g05000.t01	Wine grape	cytosol	78.35	78.35
CDY15993	Canola	cytosol	78.05	77.81
AT1G12360.1	Thale cress	cytosol	77.89	77.78
Bra026974.1-P	Field mustard	cytosol	77.74	77.63
CDY26559	Canola	cytosol	77.74	77.63
Bra019760.1-P	Field mustard	cytosol	74.44	77.59
CDX98070	Canola	cytosol	77.44	77.44
CDY30074	Canola	cytosol	77.44	77.1
Solyc08g079520.2.1	Tomato	nucleus	75.94	75.83
Os02t0452500-01	Rice	cytosol, plasma membrane	13.08	75.65
KRH65274	Soybean	cytosol	66.17	65.77
KRH76261	Soybean	nucleus	65.86	65.37
KRH64544	Soybean	endoplasmic reticulum	18.2	21.3
KRH53395	Soybean	cytosol	17.89	20.95
KRH42240	Soybean	endoplasmic reticulum, nucleus	17.89	19.13
KRH58359	Soybean	endoplasmic reticulum	17.89	19.13

Protein Annotations

Gene3D:1.25.40.60	EntrezGene:100791669	MapMan:13.4.2.4	Gene3D:3.40.50.1910	Gene3D:3.40.50.2060	Gene3D:3.90.830.10
EMBL:ACUP02002824	EnsemblPlantsGene:GLYMA_05G053100	GO:GO:0006810	GO:GO:0006904	GO:GO:0008150	GO:GO:0009987
GO:GO:0016192	UniProt:I1K0I0	InterPro:IPR027482	EnsemblPlants:KRH57307	ProteinID:KRH57307	ProteinID:KRH57307.1
PFAM:PF00995	PIRSF:PIRSF005715	PANTHER:PTHR11679	PANTHER:PTHR11679:SF59	SUPFAM:SSF56815	InterPro:Sec-1-like_dom2
InterPro:Sec1-like	InterPro:Sec1-like_sf	UniParc:UPI00023396F9	SEG:seg	:	:

Description

hypothetical protein

Coordinates

chr5:+:4766001..4780503

Molecular Weight (calculated)

75633.3 Da

IEP (calculated)

8.584

GRAVY (calculated)

-0.431

Length

665 amino acids

Sequence
(BLAST)

001: MSMSDSDSSS SYGAEYKSLK QVSRERLLHE MLRSAKTGDS KSTWKVLIMD KLTVKIMSHS CKMADITDEG VSLVEDIFKR RQPLPTMDAI YFIQPTRENI
101: IMFLSDMSGR KPLYRKAFVF FSSPIARELV MEIKKDAQVL PRIGALREMN LEYFTIDSQG FITNNERALV ELFGDEENNR KAVACLNVMA TRIATVFASL
201: REFPFVRFRA AKSLDATTMT TFHDLIPTKL AAGVWDCLMK YKKTVPNFPQ TESCELLIID RTIDQIAPVI HEWTYDAMCR DLLNMEGNKY VHEVPSKTGG
301: PPERKEVLLE DHDPIWLELR HAHIADASER LHEKMTNFIS KNKAAQIQHG SRGSGEMSTR DLQKMVQALP QYSEQIDKLS LHVEIAGKIN RIIRESGLRE
401: LGKLEQDLVF GDAGMKDVIK FLTTYEDTSR ENKLRLLMIL ASIYPEKFEG EKGLNLMKVA KLTDEDTIAI NNLRMLGGEP DTKKTSTSSF ALKFDMHKKK
501: RAARKDRSGE EETWQLSRFY PIIEELIEKV SKNELSKLDY PCLNDPSPTF HGTPYAGPVT QNPPAHSMRS RRTPTWARPR GSDDGYSSDS VLKHASSDFK
601: KMGQRIFIFI VGGATRSELR ICHKLTGKLK REVILGSSSI DDPAQYITKL KMLTAQELSL DDLQI

Best Arabidopsis Sequence Match ( AT1G12360.1 )
(BLAST)

001: MSYSDSDSSS HGGEYKNFRQ ITRERLLYEM LRSAKTGSSK STWKVLIMDK LTVKIMSYAC KMADITQEGV SLVEDIFRRR QPLPSMDAIY FIQPTKENVI
101: MFLSDMSGKS PLYKKAFVFF SSPVSKELVG HIKKDSSVLP RIGALREMNL EFFAIDSQGF ITDHERALED LFGDEETSRK GDACLNVMAS RIATVFASLR
201: EFPAVRYRAA KSLDASTMTT LRDLIPTKLA AGIWNCLAKH KQSIENFPQT ETCELLILDR SIDQIAPVIH EWTYDAMCHD LLNMEGNKYV HVIPSKSGGQ
301: PEKKDVLLEE HDPIWLELRH AHIADASERL HDKMTNFLSK NKAAQLQGKR DGAELSTRDL QKMVQALPQY SEQIDKLSLH VEIARKLNDL IREQGLRELG
401: QLEQDLVFGD AGMKDVIKYL STQEEASREG KLRLLMILAT IYPEKFEGEK GQNLMKLAKL SSDDMTAVNN MSLLGSAVDA KKNTPGGFTL KFDLHKKKRA
501: VRKERQEEAA WQLSRFYPMI EELIEKLSKG ELPKEDFPCM NDPSPSFHGS TSLSSAASSS QGQAAQSMRS RRTPTWAKPR GSDDGYSSDS VLRHASSDFR
601: KMGQRIFVFI VGGATRSELK VCHKLSTKLK REVILGSTSL DDPPQFITKL KLLTANDDLS LDDLQI

Arabidopsis Description

KEUSNARE-interacting protein KEULE [Source:UniProtKB/Swiss-Prot;Acc:Q9C5X3]
SUBAcon: [cytosol]

Hydropathy Plot

About CropPAL

The Protein Annotated Locations Database (CropPAL) houses large scale proteomic and GFP localization data from published experimental studies in Soybean (Glycine max), Maize (Zea mays), Wheat (Triticum aestivum), Barley (Hordeum vulgare), Rice (Oryza sativa), Field mustard (Brassica rapa), Canola (Brassica napus), Sorghum (Sorghum bicolor), Potato (Solanum tuberosum), Tomato (Solanum lycopersicum), Banana (Musa acuminata) and Wine grape (Vitis vinifera) as well as precomputed predictions for protein subcellular localizations using protein sequences.