Factsheet for Os02t0218800-01

Subcellular Localization

min:

: max

Winner_takes_all: plasma membrane, plastid, vacuole, cytosol

Predictor Summary:

nucleus 1
plastid 2
cytosol 3
endoplasmic reticulum 1
mitochondrion 1

Predictors

GFP

MS/MS

Papers

plastid: 16531481

vacuole: 18557835
plasma membrane: 27800704

gfp PMID: 16531481 doi

EW Chehab, G Raman, JW Walley, JV Perea, G Banu, S Theg, K Dehesh

Section of Plant Biology, University of California, Davis, California 95616, USA.

msms PMID: 27800704 doi

J Cao, C Yang, L Li, L Jiang, Y Wu, C Wu, Q Bu, G Xia, X Liu, Y Luo, J Liu

Huai'an Institute of Agricultural Sciences, Huai'an, 223001, China., Institute of Analytical Chemistry and Synthetic and Functional Biomolecules Center, College of Chemistry and Molecular Engineering, Peking University, Beijing, 100871, China; and., Northeast Institute of Geography and Agroecology, Key Laboratory of Soybean Molecular Design Breeding, Chinese Academy of Sciences, Harbin, 150081, China., State Key Laboratory of Plant Genomics, Institute of Microbiology, Chinese Academy of Sciences, Beijing, 100101, China.

msms PMID: 18557835 doi

SA Whiteman, TS Nühse, DA Ashford, D Sanders, FJ Maathuis

Department of Biology Area 9, University of York, York YO10 5DD, UK.

PPI

Inferred distinct locusB in Crop

locusB	locations
Os10t0362300-03

Inferred from Arabidopsis experimental PPI

Ath locusA	locusB	Ath locusB	Paper
AT5G42650.1	Os10t0362300-03	AT5G35750.1	18642946

Homology

Paralog

locus	Identity	Homology Identity

Ortholog

locus	Homology Species	Location	Identity	Homology Identity
Os02t0218700-01	Rice	extracellular, plasma membrane	83.33	85.0
TraesCS6D01G172200.1	Wheat	cytosol, plastid	74.12	76.99
HORVU6Hr1G039440.1	Barley	plasma membrane	73.14	75.97
EES04752	Sorghum	cytosol	75.29	73.14
TraesCS6B01G213300.1	Wheat	plastid	74.31	72.74
Zm00001d053586_P001	Maize	cytosol	72.16	72.3
TraesCS6A01G185100.1	Wheat	mitochondrion, plastid	74.71	72.02
Os03t0225900-01	Rice	mitochondrion, plastid	47.25	50.42
KRH15274	Soybean	cytosol	38.24	46.65
Os03t0767000-01	Rice	plastid	45.49	45.31
PGSC0003DMT400002934	Potato	plastid	44.31	44.31
Solyc11g069800.1.1	Tomato	plastid	43.73	43.73
KRH05758	Soybean	nucleus, plastid	44.12	43.35
Os02t0110200-01	Rice	plastid	41.18	43.12
AT5G42650.1	Thale cress	plastid	43.73	43.05
KRH61218	Soybean	nucleus, plastid	43.33	42.42
Solyc04g079730.1.1	Tomato	plastid	44.12	42.13
PGSC0003DMT400043495	Potato	plastid	43.73	42.08
VIT_18s0001g11630.t01	Wine grape	plastid	42.75	41.92
CDY02409	Canola	plastid	42.94	41.4
Bra035320.1-P	Field mustard	plastid	42.75	41.21
CDY23912	Canola	plastid	42.55	41.02
KRH38153	Soybean	plastid	7.06	28.12

Protein Annotations

Gene3D:1.10.630.10	MapMan:11.7.1.3	EntrezGene:4328743	EMBL:AK105964	ProteinID:BAD17184.1	ProteinID:BAF08222.1
ProteinID:BAS77679.1	InterPro:Cyt_P450	InterPro:Cyt_P450_E_grp-IV	InterPro:Cyt_P450_sf	GO:GO:0003674	GO:GO:0003824
GO:GO:0004497	GO:GO:0005488	GO:GO:0005506	GO:GO:0006629	GO:GO:0006631	GO:GO:0006633
GO:GO:0008150	GO:GO:0008152	GO:GO:0009058	GO:GO:0009987	GO:GO:0016125	GO:GO:0016705
GO:GO:0016829	GO:GO:0020037	GO:GO:0031408	GO:GO:0046872	GO:GO:0047987	GO:GO:0055114
InterPro:IPR036396	EnsemblPlantsGene:Os02g0218800	EnsemblPlants:Os02t0218800-01	PFAM:PF00067	PRINTS:PR00465	PANTHER:PTHR24286
PANTHER:PTHR24286:SF45	UniProt:Q6Z6K9	SUPFAM:SSF48264	UniParc:UPI000035AE31	RefSeq:XP_015623380.1	SEG:seg

Description

ALLENE OXIDE SYNTHASE 4Allene oxide synthase (CYP74A4), Fatty acid 9-/13-hydroperoxide lyase (CYP74C), Biosynthesis of jasmonic acid (JA), Plant defense (Os02t0218800-01)

Coordinates

chr2:+:6637973..6639828

Molecular Weight (calculated)

55332.8 Da

IEP (calculated)

7.470

GRAVY (calculated)

-0.001

Length

510 amino acids

Sequence
(BLAST)

001: MAPPRANSGD GNDGAVGGQS KLSPSGLLIR EIPGGYGVPF LSPLRDRLDY YYFQGADEFF RSRVARHGGA TVLRVNMPPG PFLAGDPRVV ALLDARSFRV
101: LLDDSMVDKA DTLDGTFMPS LALFGGHRPL AFLDAADPRH AKIKRVVMSL AAARMHHVAP AFRAAFAAMF DEVDAGLVAG GPVEFNKLNM RYMLDFTCAA
201: LFGGAPPSKA MGDAAVTKAV KWLIFQLHPL ASKVVKPWPL EDLLLHTFRL PPFLVRREYG EITAYFAAAA AAILDDAEKN HPGIPRDELL HNLVFVAVFN
301: AYGGFKIFLP HIVKWLARAG PELHAKLASE VRAAAPAGGG EITISAVEKE MPLVKSVVWE ALRMNPPVEF QYGRARRDMV VESHDAAYEV RKGELLFGYQ
401: PLATRDEKVF DRAGEFVPDR FVSGAGSAAR PLLEHVVWSN GPETGTPSEG NKQCPGKDMV VAVGRLMVAG LFRRYDTFAA DVEELPLEPV VTFTSLTRAA
501: DGDGAARRGV

Best Arabidopsis Sequence Match ( AT5G42650.1 )
(BLAST)

001: MASISTPFPI SLHPKTVRSK PLKFRVLTRP IKASGSETPD LTVATRTGSK DLPIRNIPGN YGLPIVGPIK DRWDYFYDQG AEEFFKSRIR KYNSTVYRVN
101: MPPGAFIAEN PQVVALLDGK SFPVLFDVDK VEKKDLFTGT YMPSTELTGG YRILSYLDPS EPKHEKLKNL LFFLLKSSRN RIFPEFQATY SELFDSLEKE
201: LSLKGKADFG GSSDGTAFNF LARAFYGTNP ADTKLKADAP GLITKWVLFN LHPLLSIGLP RVIEEPLIHT FSLPPALVKS DYQRLYEFFL ESAGEILVEA
301: DKLGISREEA THNLLFATCF NTWGGMKILF PNMVKRIGRA GHQVHNRLAE EIRSVIKSNG GELTMGAIEK MELTKSVVYE CLRFEPPVTA QYGRAKKDLV
401: IESHDAAFKV KAGEMLYGYQ PLATRDPKIF DRADEFVPER FVGEEGEKLL RHVLWSNGPE TETPTVGNKQ CAGKDFVVLV ARLFVIEIFR RYDSFDIEVG
501: TSPLGSSVNF SSLRKASF

Arabidopsis Description

CYP74AAllene oxide synthase, chloroplastic [Source:UniProtKB/Swiss-Prot;Acc:Q96242]
SUBAcon: [plastid]

Hydropathy Plot

About CropPAL

The Protein Annotated Locations Database (CropPAL) houses large scale proteomic and GFP localization data from published experimental studies in Soybean (Glycine max), Maize (Zea mays), Wheat (Triticum aestivum), Barley (Hordeum vulgare), Rice (Oryza sativa), Field mustard (Brassica rapa), Canola (Brassica napus), Sorghum (Sorghum bicolor), Potato (Solanum tuberosum), Tomato (Solanum lycopersicum), Banana (Musa acuminata) and Wine grape (Vitis vinifera) as well as precomputed predictions for protein subcellular localizations using protein sequences.