Factsheet for Bra030528.1-P

Subcellular Localization

min:

: max

Winner_takes_all: cytosol

Predictor Summary:

nucleus 2
cytosol 2
plastid 1
mitochondrion 1

Predictors

GFP

MS/MS

Papers

Winner Takes All:	cytosol
Any Predictor:	cytosol, mitochondrion, nucleus, plastid
BaCelLo:	nucleus
MultiLoc:	cytosol
Plant-mPloc:	plastid
PProwler:	mitochondrion
WoLF PSORT:	nucleus
YLoc:	cytosol

PPI

No PPI Data

Homology

Paralog

locus	Identity	Homology Identity

Ortholog

locus	Homology Species	Location	Identity	Homology Identity
CDY15458	Canola	cytosol	89.27	89.63
Bra033388.1-P	Field mustard	plastid	77.17	66.98
AT1G03310.2	Thale cress	plastid	77.85	64.97
GSMUA_Achr6P16200_001	Banana	cytosol, plasma membrane, vacuole	35.46	51.28
HORVU1Hr1G062690.3	Barley	cytosol	49.32	50.49
GSMUA_Achr6P16190_001	Banana	cytosol, plasma membrane, vacuole	51.22	49.87
VIT_07s0104g00370.t01	Wine grape	plasma membrane, plastid, vacuole	57.47	47.96
KRH67160	Soybean	plastid	56.11	47.69
Os05t0393700-01	Rice	cytosol, plastid	48.1	47.64
KRG95479	Soybean	mitochondrion	55.16	46.94
TraesCS1A01G247100.1	Wheat	mitochondrion, plastid	50.14	46.53
OQU77947	Sorghum	plastid	50.54	46.5
TraesCS1B01G257700.1	Wheat	mitochondrion, plastid	49.46	46.19
TraesCS1D01G246300.1	Wheat	plastid	49.18	45.88
PGSC0003DMT400002502	Potato	plastid	53.8	45.1
Solyc09g064800.1.1	Tomato	plastid	52.99	45.03
Zm00001d038121_P001	Maize	plastid	50.41	42.21
Bra000667.1-P	Field mustard	plastid	30.16	30.83
Bra014108.1-P	Field mustard	cytosol	7.34	26.09
Bra005001.1-P	Field mustard	plastid	28.53	25.99
Bra014111.1-P	Field mustard	mitochondrion	4.76	24.48
Bra040385.1-P	Field mustard	cytosol, plasma membrane, plastid	8.02	7.87
Bra009461.1-P	Field mustard	plastid	11.28	6.22

Protein Annotations

Gene3D:2.60.40.10	Gene3D:2.60.40.1180	MapMan:3.2.2.6	MapMan:3.2.3.3.2.1	Gene3D:3.20.20.80	EnsemblPlantsGene:Bra030528
EnsemblPlants:Bra030528.1	EnsemblPlants:Bra030528.1-P	GO:GO:0003674	GO:GO:0003824	GO:GO:0004553	GO:GO:0005975
GO:GO:0008150	GO:GO:0008152	GO:GO:0016787	InterPro:Glyco_hydro_13_N	InterPro:Glyco_hydro_13_cat_dom	InterPro:Glyco_hydro_b
InterPro:Glycoside_hydrolase_SF	InterPro:IPR013780	InterPro:IPR013783	InterPro:Ig-like_fold	InterPro:Ig_E-set	UniProt:M4EP08
PFAM:PF00128	PFAM:PF02922	PANTHER:PTHR43002	PANTHER:PTHR43002:SF2	SMART:SM00642	SUPFAM:SSF51011
SUPFAM:SSF51445	SUPFAM:SSF81296	UniParc:UPI0002545A9B	SEG:seg	:	:

Description

AT1G03310 (E=0.0) ATISA2, ISA2, DBE1, BE2 | isoamylase, putative / starch debranching enzyme, putative

Coordinates

chrA08:-:21449015..21451225

Molecular Weight (calculated)

82854.8 Da

IEP (calculated)

5.462

GRAVY (calculated)

-0.313

Length

736 amino acids

Sequence
(BLAST)

001: MVYVSSLEPN GADNKSSLVM VWGVYRSDSS CFLPLDFENS DQDSQTPFLK RSLSELMLEL EFDGKESPFY LSFRLKLVSA NDPNGLEMLS HRDTNFCVPV
101: GFTAGHPLPL GLSSGPDGGG SWNFAFFSRN SKSLVLCLYD DSTTNRPALE LDLDPYVNRT GDVWHASVDK TWGFVRYGYR CKESTNSEDD EAESIVLDPY
201: ARVIEKTISP KFLGSISKNS SFDWGRDVSP NIPLEKLIVY RLNVKDFTQH KSSKLPTNVA GTFSGVAEKV NHLKALGINA VLLEPIFSFS EQKGPYFPFH
301: FFSPTDMYGH ESAVNSMKEV VKRLHSEGIE VLLEVVFTHT AESGALQGID DSSYYYNGKD SKSYLNCNYP VVQQLILESL RYWVTEFHVD GFCFINASSL
401: LRGVHGEQLS RPPLVEAISF DPQLARTKLI ADCWDPHDMK MPKEVKFPHW KRWAELNTRY CRDVRNFVRG RGVLSDLATR VCGSGDIFTD GRGPAFSFNY
501: VSRNSGLSLV DLVSFSGPEL ASEVSWNCGE EGETNKSAVL QTRLKQIRNL LFIQYISVGI PVLNMGDECG VSTNGSPLLE SRKPFDWNML ASGFGAQITQ
601: FISFMTSVRA RRSDVFQRSK FLKPKNIVWY ANDQTTPNWE DTASRFLALR IRAESEEETT EPKNNDLFIG FNASDHPEKV TLPTLPEGRS KWRRLVDTSL
701: PFPGFFSVEG QTVVTKDELV VYEMKPYSCT LFETTA

Best Arabidopsis Sequence Match ( AT1G03310.2 )
(BLAST)

001: MAAWSPSVGI GSCCLNNGIT RTWKFPSARL FTGRKNKIKL GSETLMFTRK RFMGDLVTSA LQSYQFSKIC ASKTSIELRE ALSSRRAEAD DLKKVTSYSF
101: RTKAGALVKV KVEKKREKYS ILVYVSSLEL SGDDKSRLVM VWGVYRSDSS CFLPLDFENS SQDSQTHTTE TTFVKSSLSE LMLGLEFDGK ESPFYLSFHL
201: KLVSGRDPDG QEMLTHRDTD FCIPVGFTAG HPLPLGLSSG PDDDSWNFSF FSRSSTNVVL CLYDDSTTDK PALELDLDPY VNRTGDVWHA SVDNTWDFVR
301: YGYRCKETAH SKEDVDVEGE PIVLDPYATV VGKSVSQKYL GSLSKSPSFD WGEDVSPNIP LEKLLVYRLN VKGFTQHRSS KLPSNVAGTF SGVAEKVSHL
401: KTLGTNAVLL EPIFSFSEQK GPYFPFHFFS PMDIYGPSNS LESAVNSMKV MVKKLHSEGI EVLLEVVFTH TADSGALRGI DDSSYYYKGR ANDLDSKSYL
501: NCNYPVVQQL VLESLRYWVT EFHVDGFCFI NASSLLRGVH GEQLSRPPLV EAIAFDPLLA ETKLIADCWD PLEMMPKEVR FPHWKRWAEL NTRYCRNVRN
601: FLRGRGVLSD LATRICGSGD VFTDGRGPAF SFNYISRNSG LSLVDIVSFS GPELASELSW NCGEEGATNK SAVLQRRLKQ IRNFLFIQYI SLGVPVLNMG
701: DECGISTRGS PLLESRKPFD WNLLASAFGT QITQFISFMT SVRARRSDVF QRRDFLKPEN IVWYANDQTT PKWEDPASKF LALEIKSESE EEETASLAEP
801: NEPKSNDLFI GFNASDHPES VVLPSLPDGS KWRRLVDTAL PFPGFFSVEG ETVVAEEPLQ QLVVYEMKPY SCTLFETINT TA

Arabidopsis Description

ISA2ISA2 [Source:UniProtKB/TrEMBL;Acc:A0A178WGA8]
SUBAcon: [plastid]

Hydropathy Plot

About CropPAL

The Protein Annotated Locations Database (CropPAL) houses large scale proteomic and GFP localization data from published experimental studies in Soybean (Glycine max), Maize (Zea mays), Wheat (Triticum aestivum), Barley (Hordeum vulgare), Rice (Oryza sativa), Field mustard (Brassica rapa), Canola (Brassica napus), Sorghum (Sorghum bicolor), Potato (Solanum tuberosum), Tomato (Solanum lycopersicum), Banana (Musa acuminata) and Wine grape (Vitis vinifera) as well as precomputed predictions for protein subcellular localizations using protein sequences.