Factsheet for Os05t0393700-01

Subcellular Localization

min:

: max

Winner_takes_all: plastid, cytosol

Predictor Summary:

nucleus 1
cytosol 2
plastid 1
mitochondrion 1

Predictors

GFP

MS/MS

Papers

Winner Takes All:	cytosol, plastid
Any Predictor:	cytosol, mitochondrion, nucleus, plastid
BaCelLo:	nucleus
MultiLoc:	cytosol
Plant-mPloc:	plastid
PProwler:	mitochondrion
YLoc:	cytosol

plastid: 27992503

msms PMID: 27992503 doi

S Xing, X Meng, L Zhou, H Mujahid, C Zhao, Y Zhang, C Wang, Z Peng

Department of Biochemistry, Molecular Biology, Entomology and Plant Pathology, Mississippi State University, Starkville, Mississippi, United States of America., Institute of Food Crops, Jiangsu Academy of Agricultural Sciences, Jiangsu High Quality Rice Research and Development Center, Nanjing Branch of China National Center for Rice Improvement, Nanjing, Jiangsu, China.

PPI

No PPI Data

Homology

Paralog

locus	Identity	Homology Identity

Ortholog

locus	Homology Species	Location	Identity	Homology Identity
HORVU1Hr1G062690.3	Barley	cytosol	72.68	75.1
OQU77947	Sorghum	plastid	76.58	71.12
TraesCS1D01G246300.1	Wheat	plastid	73.89	69.58
TraesCS1B01G257700.1	Wheat	mitochondrion, plastid	73.35	69.16
TraesCS1A01G247100.1	Wheat	mitochondrion, plastid	73.62	68.98
Zm00001d038121_P001	Maize	plastid	74.83	63.25
GSMUA_Achr6P16200_001	Banana	cytosol, plasma membrane, vacuole	36.34	53.05
GSMUA_Achr6P16190_001	Banana	cytosol, plasma membrane, vacuole	51.01	50.13
Bra030528.1-P	Field mustard	cytosol	47.64	48.1
CDY15458	Canola	cytosol	47.11	47.75
KRG95479	Soybean	mitochondrion	51.14	43.93
VIT_07s0104g00370.t01	Wine grape	plasma membrane, plastid, vacuole	52.09	43.88
CDY18165	Canola	plastid	47.91	43.52
KRH67160	Soybean	plastid	50.07	42.96
CDX89902	Canola	plastid	48.32	42.64
Solyc09g064800.1.1	Tomato	plastid	48.72	41.8
PGSC0003DMT400002502	Potato	plastid	49.26	41.69
Bra033388.1-P	Field mustard	plastid	47.24	41.39
AT1G03310.2	Thale cress	plastid	48.05	40.48
CDX86680	Canola	plastid	44.28	39.73
Os09t0469400-01	Rice	plastid	14.13	30.97
Os08t0520900-00	Rice	cytosol, plastid	29.74	30.48
Os04t0164900-01	Rice	cytosol, nucleus, plastid	9.42	12.61

Protein Annotations

Gene3D:2.60.40.10	Gene3D:2.60.40.1180	MapMan:3.2.2.6	MapMan:3.2.3.3.2.1	Gene3D:3.20.20.80	GO:GO:0003674
GO:GO:0003824	GO:GO:0004553	GO:GO:0005575	GO:GO:0005622	GO:GO:0005623	GO:GO:0005737
GO:GO:0005975	GO:GO:0005977	GO:GO:0005983	GO:GO:0006091	GO:GO:0008150	GO:GO:0008152
GO:GO:0009056	GO:GO:0009058	GO:GO:0009507	GO:GO:0009536	GO:GO:0009987	GO:GO:0010021
GO:GO:0016787	GO:GO:0016798	GO:GO:0019156	GO:GO:0019252	GO:GO:0043033	InterPro:Glyco_hydro_13_N
InterPro:Glyco_hydro_13_cat_dom	InterPro:Glyco_hydro_b	InterPro:Glycoside_hydrolase_SF	InterPro:IPR013780	InterPro:IPR013783	InterPro:Ig-like_fold
InterPro:Ig_E-set	EnsemblPlantsGene:Os05g0393700	EnsemblPlants:Os05t0393700-01	PFAM:PF00128	PFAM:PF02922	PANTHER:PTHR43002
PANTHER:PTHR43002:SF2	SMART:SM00642	SUPFAM:SSF51011	SUPFAM:SSF51445	SUPFAM:SSF81296	UniParc:UPI000393B4D5
SEG:seg	:	:	:	:	:

Description

ISOAMYLASE 2Similar to Isoamylase-type starch debranching enzyme ISO2. (Os05t0393700-01)

Coordinates

chr5:-:19155053..19157429

Molecular Weight (calculated)

80695.9 Da

IEP (calculated)

6.207

GRAVY (calculated)

-0.003

Length

743 amino acids

Sequence
(BLAST)

001: DGDGGGGGYA VSVEVPGTRG REGGLVLRAS GSGEGVPLAP AAGGASLAAE LSFDPTRAPF YLSFLLTDAS GAEIRTHRKT SFRVPVGVGP GSPAPLGMSI
101: SGDGAVNFAV YSKNANAVSL YLYAAAVGGG GGDEPALEID LDPYIHRTGN VWHVSLASVD GYVSYAFCCG GIRRPLLDPY AKVIGDFVSS NSVYDEGVTA
201: PSMRCFASLA IAPSYNWGRD RHPRLPLEKL VVYRANVALF TKDRSSGLPD DAAGTFTGLS AKVEHFRSLG VNAILLEPVF PFHQVKGPYF PYHFFSPMNL
301: YSSKGLSVSA IKSMKDMVRV MHRNGIEVLL EVVFTHTAEG ESECQTISMR GIDNSSYYIA NGIAGCKASI LNCNHPVTQK LILDSLRHWV LDFHVDGFCF
401: INAPFLVRGP GGEYLSRPPL LEAITFDPVL SMTKIIADPW SPLDISNVQF PFPHWKRWAE VNTRFSIDVR KFLKREALIS DLATRLCGSG DLFSTRGPAF
501: SFNHVSRNSG LSLVDLVSFS NDDLLSESSW NCGEEGPSEN SAVLQTRLRQ IRNFLFILFV SLGVPVLNMG DECGHSAAGS VSYKDRGPLN WRGMKTTFVK
601: EVTGFISFLT ALRSRRGDIF QRREFLKLEN IHWYGSDLCE PGWDDPTSNF LCMHINAEVD EMAADSVRGD LYICFNANEE SVSAALPALA EGSVWLRLVD
701: TSLAFPGFFA TESNPKVQQV PGLSSYHVEA HTCVLFESKS ALA

Best Arabidopsis Sequence Match ( AT1G03310.2 )
(BLAST)

001: MAAWSPSVGI GSCCLNNGIT RTWKFPSARL FTGRKNKIKL GSETLMFTRK RFMGDLVTSA LQSYQFSKIC ASKTSIELRE ALSSRRAEAD DLKKVTSYSF
101: RTKAGALVKV KVEKKREKYS ILVYVSSLEL SGDDKSRLVM VWGVYRSDSS CFLPLDFENS SQDSQTHTTE TTFVKSSLSE LMLGLEFDGK ESPFYLSFHL
201: KLVSGRDPDG QEMLTHRDTD FCIPVGFTAG HPLPLGLSSG PDDDSWNFSF FSRSSTNVVL CLYDDSTTDK PALELDLDPY VNRTGDVWHA SVDNTWDFVR
301: YGYRCKETAH SKEDVDVEGE PIVLDPYATV VGKSVSQKYL GSLSKSPSFD WGEDVSPNIP LEKLLVYRLN VKGFTQHRSS KLPSNVAGTF SGVAEKVSHL
401: KTLGTNAVLL EPIFSFSEQK GPYFPFHFFS PMDIYGPSNS LESAVNSMKV MVKKLHSEGI EVLLEVVFTH TADSGALRGI DDSSYYYKGR ANDLDSKSYL
501: NCNYPVVQQL VLESLRYWVT EFHVDGFCFI NASSLLRGVH GEQLSRPPLV EAIAFDPLLA ETKLIADCWD PLEMMPKEVR FPHWKRWAEL NTRYCRNVRN
601: FLRGRGVLSD LATRICGSGD VFTDGRGPAF SFNYISRNSG LSLVDIVSFS GPELASELSW NCGEEGATNK SAVLQRRLKQ IRNFLFIQYI SLGVPVLNMG
701: DECGISTRGS PLLESRKPFD WNLLASAFGT QITQFISFMT SVRARRSDVF QRRDFLKPEN IVWYANDQTT PKWEDPASKF LALEIKSESE EEETASLAEP
801: NEPKSNDLFI GFNASDHPES VVLPSLPDGS KWRRLVDTAL PFPGFFSVEG ETVVAEEPLQ QLVVYEMKPY SCTLFETINT TA

Arabidopsis Description

ISA2ISA2 [Source:UniProtKB/TrEMBL;Acc:A0A178WGA8]
SUBAcon: [plastid]

Hydropathy Plot

About CropPAL

The Protein Annotated Locations Database (CropPAL) houses large scale proteomic and GFP localization data from published experimental studies in Soybean (Glycine max), Maize (Zea mays), Wheat (Triticum aestivum), Barley (Hordeum vulgare), Rice (Oryza sativa), Field mustard (Brassica rapa), Canola (Brassica napus), Sorghum (Sorghum bicolor), Potato (Solanum tuberosum), Tomato (Solanum lycopersicum), Banana (Musa acuminata) and Wine grape (Vitis vinifera) as well as precomputed predictions for protein subcellular localizations using protein sequences.