Factsheet for Os09t0469400-01

Subcellular Localization

min:

: max

Winner_takes_all: plastid

Predictor Summary:

cytosol 3
vacuole 1
plastid 1
mitochondrion 1

Predictors

GFP

MS/MS

Papers

Winner Takes All:	plastid
Any Predictor:	cytosol, mitochondrion, plastid, vacuole
BaCelLo:	cytosol
MultiLoc:	vacuole
Plant-mPloc:	plastid
PProwler:	mitochondrion
WoLF PSORT:	cytosol
YLoc:	cytosol

plastid: 21551159

plastid: 17189339

gfp PMID: 21551159 doi

MS Yun, T Umemoto, Y Kawagoe

Division of Plant Sciences, National Institute of Agrobiological Sciences, Kannondai, Tsukuba, Japan.

msms PMID: 17189339 doi

T Kleffmann, A von Zychlinski, D Russenberger, M Hirsch-Hoffmann, P Gehrig, W Gruissem, S Baginsky

Institute of Plant Sciences, Eidgenössische Technische Hochschule Zurich, 8092 Zurich, Switzerland.

PPI

No PPI Data

Homology

Paralog

locus	Identity	Homology Identity

Ortholog

locus	Homology Species	Location	Identity	Homology Identity
Zm00001d005949_P001	Maize	cytosol, mitochondrion, vacuole	62.24	75.63
CDY70211	Canola	plastid	64.9	39.86
KXG35819	Sorghum	plastid	78.76	34.41
TraesCS5D01G255800.1	Wheat	plastid	78.17	34.15
HORVU5Hr1G070160.1	Barley	plastid	76.99	33.63
TraesCS5B01G246400.1	Wheat	plastid	76.7	33.51
TraesCS5A01G248700.1	Wheat	plastid	75.81	33.16
GSMUA_Achr9P14980_001	Banana	cytosol	67.55	32.03
Zm00001d020799_P003	Maize	mitochondrion	75.52	31.92
Solyc06g009220.2.1	Tomato	plastid	66.96	30.72
KRH53006	Soybean	plastid	68.44	30.05
Bra000667.1-P	Field mustard	plastid	63.42	29.86
AT4G09020.1	Thale cress	plastid	67.26	29.84
CDX90708	Canola	plastid	65.49	29.52
KRH62296	Soybean	plastid	68.14	29.5
VIT_18s0001g06520.t01	Wine grape	plastid	67.26	29.42
PGSC0003DMT400018765	Potato	cytosol	19.76	29.26
Os08t0520900-00	Rice	cytosol, plastid	40.12	18.76
Os05t0393700-01	Rice	cytosol, plastid	30.97	14.13
CDY60217	Canola	extracellular	3.24	6.4
Os04t0164900-01	Rice	cytosol, nucleus, plastid	8.85	5.41
CDX77349	Canola	mitochondrion	5.31	4.62
PGSC0003DMT400018766	Potato	cytosol	1.77	2.43
Bra014111.1-P	Field mustard	mitochondrion	0.59	1.4
Bra014108.1-P	Field mustard	cytosol	0.59	0.97
CDY05042	Canola	cytosol	0.0	0.0
CDX77333	Canola	cytosol	0.0	0.0

Protein Annotations

Gene3D:2.60.40.10	MapMan:3.2.2.6	MapMan:3.2.3.3.2.1	Gene3D:3.20.20.80	GO:GO:0003674	GO:GO:0003824
GO:GO:0004553	GO:GO:0005575	GO:GO:0005622	GO:GO:0005623	GO:GO:0005737	GO:GO:0005975
GO:GO:0005977	GO:GO:0005983	GO:GO:0006091	GO:GO:0008150	GO:GO:0008152	GO:GO:0009056
GO:GO:0009058	GO:GO:0009501	GO:GO:0009507	GO:GO:0009536	GO:GO:0009660	GO:GO:0009987
GO:GO:0016043	GO:GO:0016787	GO:GO:0016798	GO:GO:0019156	GO:GO:0019252	InterPro:Glyco_hydro_13_N
InterPro:Glyco_hydro_13_cat_dom	InterPro:Glycoside_hydrolase_SF	InterPro:IPR013783	InterPro:Ig-like_fold	EnsemblPlantsGene:Os09g0469400	EnsemblPlants:Os09t0469400-01
PFAM:PF00128	PFAM:PF02922	PANTHER:PTHR43002	PANTHER:PTHR43002:SF3	SUPFAM:SSF51445	UniParc:UPI0003939965

Description

ISOAMYLASE 3Similar to isoamylase-type starch debranching enzyme ISO3. (Os09t0469400-01);Similar to isoamylase-type starch debranching enzyme ISO3. (Os09t0469400-02)

Coordinates

chr9:-:17857352..17868668

Molecular Weight (calculated)

37701.5 Da

IEP (calculated)

4.689

GRAVY (calculated)

-0.288

Length

339 amino acids

Sequence
(BLAST)

001: MTEERGCTMS DTEMPFKYSS GKAFPLGVSQ VEGGLNFALF SQHASSVILC LKLPGRGTED EKGADVVEFV LDQQKNKTGD IWHVIVEGLP ASGVLYGYRV
101: GGPQGWDQGH RFDSSTVLLD PYAKLVSGRK YFGVAEEKSS QHFGTYDFDS SPFDWGDDYR LPNLPEADLV IYEMNVRAFT ADESSGLDST SRGSYLGLID
201: KIPHLLELGV NAVELLPVFE YDELEFKRYP NPRDHMVNTW GYSTINFFAP MSRYASAGGG PVAASKELKQ MVKELHKAGI EVILDVVYNH TNEADDAHPY
301: MTSFRGIDNK VYYMLDLNKN AELLNFSGCG KTMVPVLVY

Best Arabidopsis Sequence Match ( AT4G09020.1 )
(BLAST)

001: MLTSPSSSST YDPFSSNFSP SLTNAFSSSF TIPMGLKLSR RVTRARIFSR KIKDRSTLKV TCRRAHERVV EEEASTMTET KLFKVSSGEV SPLGVSQVDK
101: GINFALFSQN ATSVTLCLSL SQSGKDDTDD DGMIELVLDP SVNKTGDTWH ICVEDLPLNN VLYGYRVDGP GEWQQGHRFD RSILLLDPYA KLVKGHSSFG
201: DSSQKFAQFY GTYDFESSPF DWGDDYKFPN IPEKDLVIYE MNVRAFTADE SSGMDPAIGG SYLGFIEKIP HLQDLGINAV ELLPVFEFDE LELQRRSNPR
301: DHMVNTWGYS TVNFFAPMSR YASGEGDPIK ASKEFKEMVK ALHSAGIEVI LDVVYNHTNE ADDKYPYTTS FRGIDNKVYY MLDPNNQLLN FSGCGNTLNC
401: NHPVVMELIL DSLRHWVTEY HVDGFRFDLA SVLCRATDGS PLSAPPLIRA IAKDSVLSRC KIIAEPWDCG GLYLVGKFPN WDRWAEWNGM YRDDVRRFIK
501: GDSGMKGSFA TRVSGSSDLY QVNQRKPYHG VNFVIAHDGF TLRDLVSYNF KHNEANGEGG NDGCNDNHSW NCGFEGETGD AHIKSLRTRQ MKNFHLALMI
601: SQGTPMMLMG DEYGHTRYGN NNSYGHDTSL NNFQWKELDA KKQNHFRFFS EVIKFRHSHH VLKHENFLTQ GEITWHEDNW DNSESKFLAF TLHDGIGGRD
701: IYVAFNAHDY FVKALIPQPP PGKQWFRVAD TNLESPDDFV REGVAGVADT YNVAPFSSIL LQSK

Arabidopsis Description

ISA3Isoamylase 3, chloroplastic [Source:UniProtKB/Swiss-Prot;Acc:Q9M0S5]
SUBAcon: [plastid]

Hydropathy Plot

About CropPAL

The Protein Annotated Locations Database (CropPAL) houses large scale proteomic and GFP localization data from published experimental studies in Soybean (Glycine max), Maize (Zea mays), Wheat (Triticum aestivum), Barley (Hordeum vulgare), Rice (Oryza sativa), Field mustard (Brassica rapa), Canola (Brassica napus), Sorghum (Sorghum bicolor), Potato (Solanum tuberosum), Tomato (Solanum lycopersicum), Banana (Musa acuminata) and Wine grape (Vitis vinifera) as well as precomputed predictions for protein subcellular localizations using protein sequences.