Factsheet for Os08t0520900-00

Subcellular Localization

min:

: max

Winner_takes_all: plastid, cytosol

Predictor Summary:

extracellular 1
endoplasmic reticulum 1
vacuole 2
plasma membrane 1
golgi 1
mitochondrion 2
plastid 1
peroxisome 1

Predictors

GFP

MS/MS

Papers

Winner Takes All:	cytosol, plastid
Any Predictor:	mitochondrion, peroxisome, plastid, secretory
BaCelLo:	secretory
iPSORT:	mitochondrion
MultiLoc:	vacuole
Plant-mPloc:	plastid
PProwler:	mitochondrion
YLoc:	peroxisome

cytosol: 19685898
plastid: 27992503

msms PMID: 19685898 doi

KA Doroshenk, AJ Crofts, RT Morris, JJ Wyrick, TW Okita

Institute of Biological Chemistry, Washington State University, Pullman, Washington 99164-6340, USA.

msms PMID: 27992503 doi

S Xing, X Meng, L Zhou, H Mujahid, C Zhao, Y Zhang, C Wang, Z Peng

Department of Biochemistry, Molecular Biology, Entomology and Plant Pathology, Mississippi State University, Starkville, Mississippi, United States of America., Institute of Food Crops, Jiangsu Academy of Agricultural Sciences, Jiangsu High Quality Rice Research and Development Center, Nanjing Branch of China National Center for Rice Improvement, Nanjing, Jiangsu, China.

PPI

No PPI Data

Homology

Paralog

locus	Identity	Homology Identity

Ortholog

locus	Homology Species	Location	Identity	Homology Identity
TraesCS7B01G139700.2	Wheat	plastid	85.66	78.61
TraesCS7A01G251400.1	Wheat	mitochondrion, plastid	85.38	78.35
TraesCS7D01G249500.1	Wheat	mitochondrion, plastid	85.1	78.0
PGSC0003DMT400053345	Potato	cytosol	30.9	77.24
KXG25605	Sorghum	plastid	82.9	75.88
VIT_11s0078g00310.t01	Wine grape	extracellular, golgi	55.31	74.4
HORVU7Hr1G051710.1	Barley	vacuole	50.21	72.22
GSMUA_Achr9P14020_001	Banana	plasma membrane, vacuole	66.07	68.62
KRH41413	Soybean	plastid	73.24	66.54
Zm00001d049753_P001	Maize	mitochondrion, plastid	76.0	66.07
Solyc07g014590.2.1	Tomato	extracellular	71.59	65.95
AT2G39930.1	Thale cress	plastid	70.34	65.13
CDX74820	Canola	plastid	69.38	63.91
CDY20500	Canola	plastid	68.97	63.78
Bra005001.1-P	Field mustard	plastid	69.24	62.13
Zm00001d052844_P001	Maize	cytosol	10.9	46.2
VIT_11s0078g00380.t01	Wine grape	cytosol	18.76	44.01
Os09t0469400-01	Rice	plastid	18.76	40.12
Os05t0393700-01	Rice	cytosol, plastid	30.48	29.74
Zm00001d050532_P001	Maize	extracellular	11.86	21.18
Zm00001d016045_P001	Maize	plastid	10.07	19.78
Zm00001d024985_P004	Maize	plastid	4.69	15.11
Os04t0164900-01	Rice	cytosol, nucleus, plastid	10.48	13.69

Protein Annotations

Gene3D:2.60.40.10	Gene3D:2.60.40.1180	MapMan:3.2.2.6	MapMan:3.2.3.3.2.1	Gene3D:3.20.20.80	GO:GO:0003674
GO:GO:0003824	GO:GO:0004553	GO:GO:0005488	GO:GO:0005515	GO:GO:0005575	GO:GO:0005622
GO:GO:0005623	GO:GO:0005737	GO:GO:0005975	GO:GO:0005977	GO:GO:0005983	GO:GO:0006091
GO:GO:0008150	GO:GO:0008152	GO:GO:0009056	GO:GO:0009058	GO:GO:0009507	GO:GO:0009536
GO:GO:0009987	GO:GO:0010021	GO:GO:0010368	GO:GO:0016787	GO:GO:0016798	GO:GO:0019156
GO:GO:0019252	GO:GO:0043033	InterPro:Glyco_hydro_13_N	InterPro:Glyco_hydro_13_cat_dom	InterPro:Glyco_hydro_b	InterPro:Glycoside_hydrolase_SF
InterPro:IPR013780	InterPro:IPR013783	InterPro:Ig-like_fold	InterPro:Ig_E-set	EnsemblPlantsGene:Os08g0520900	EnsemblPlants:Os08t0520900-00
PFAM:PF00128	PFAM:PF02922	PANTHER:PTHR43002	PANTHER:PTHR43002:SF1	SMART:SM00642	SUPFAM:SSF51011
SUPFAM:SSF51445	SUPFAM:SSF81296	UniParc:UPI0000DD95A9	SEG:seg	:	:

Description

ISOAMYLASE 1Similar to Isoamylase (Fragment). (Os08t0520900-00)

Coordinates

chr8:-:25893657..25900342

Molecular Weight (calculated)

81623.3 Da

IEP (calculated)

5.445

GRAVY (calculated)

-0.322

Length

725 amino acids

Sequence
(BLAST)

001: VEAVVMPERY ALGGACRVLA GMPAPLGATA LDGGVNFAVY SAGASAASLC LFTPDDLEAD EVTEEVPLDP LFNRTGNVWH VFIEGELHNM LYGYRFDGMF
101: APHCGQYFDV SNVVVDPYAK AVISRGEYGV PGPGGDCWPQ MAGMIPLPYS TFDWQGDLPL RYPQKDLVIY EMHLRGFTKH SSSNVEHPGT YIGAISKLDY
201: LKELGVNCVE LMPCHEFNEL EYFSCSSKMN FWGYSTINFF SPMIRYSSGG IRNCGRDAIN EFKTFVREAH KRGIEVIMDV VFNHTAEGNE KGPILSFRGI
301: DNSTYYMLAP KGEFYNYSGC GNTFNCNHPV VREFIVDCLR YWVTEMHVDG FRFDLASIMT RGCSLWDPVN VYGSPVEGDM TTTGTPLATP PLIDMISNDP
401: ILGDVKLIAE AWDAGGLYQV GQFPHWKIWS EWNGKYRDIV RQFIKGTDGF AGGFAECLCG SPHLYQAGGR KPWHSINFVC AHDGFTLADL VTYNKKYNSS
501: NGEDNRDGEN HNLSWNCGEE GEFAGLSVKR LRKRQMRNFF VSLMVSQGVP MFYMGDEYGH TKGGNNNTYC HDHYVNYFRW DKKEESSDLQ RFCSLMTKFR
601: KQCESLGLAD FPTAQRLHWH GHQPGKPDWS ETSRFVAFST KDETKGEIYV AFNASHLPAV VGLPERPGYR WEPLVDTGKP APYDFLTDDL PDRAHAVHLF
701: SHFLNSNLYP MLSYSSIILE LQPDD

Best Arabidopsis Sequence Match ( AT2G39930.1 )
(BLAST)

001: MDAIKCSSSF LHHTKLNTLF SNHTFPKISA PNFKPLFRPI SISAKDRRSN EAENIAVVEK PLKSDRFFIS DGLPSPFGPT VRDDGVNFSV YSTNSVSATI
101: CLISLSDLRQ NKVTEEIQLD PSRNRTGHVW HVFLRGDFKD MLYGYRFDGK FSPEEGHYYD SSNILLDPYA KAIISRDEFG VLGPDDNCWP QMACMVPTRE
201: EEFDWEGDMH LKLPQKDLVI YEMHVRGFTR HESSKIEFPG TYQGVAEKLD HLKELGINCI ELMPCHEFNE LEYYSYNTIL GDHRVNFWGY STIGFFSPMI
301: RYASASSNNF AGRAINEFKI LVKEAHKRGI EVIMDVVLNH TAEGNEKGPI FSFRGVDNSV YYMLAPKGEF YNYSGCGNTF NCNHPVVRQF ILDCLRYWVT
401: EMHVDGFRFD LGSIMSRSSS LWDAANVYGA DVEGDLLTTG TPISCPPVID MISNDPILRG VKLIAEAWDA GGLYQVGMFP HWGIWSEWNG KFRDVVRQFI
501: KGTDGFSGAF AECLCGSPNL YQGGRKPWHS INFICAHDGF TLADLVTYNN KNNLANGEEN NDGENHNYSW NCGEEGDFAS ISVKRLRKRQ MRNFFVSLMV
601: SQGVPMIYMG DEYGHTKGGN NNTYCHDNYM NYFRWDKKEE AHSDFFRFCR ILIKFRDECE SLGLNDFPTA KRLQWHGLAP EIPNWSETSR FVAFSLVDSV
701: KKEIYVAFNT SHLATLVSLP NRPGYRWEPF VDTSKPSPYD CITPDLPERE TAMKQYRHFL DANVYPMLSY SSIILLLSPI KDP

Arabidopsis Description

ISA1ISA1 [Source:UniProtKB/TrEMBL;Acc:A0A178VW25]
SUBAcon: [plastid]

Hydropathy Plot

About CropPAL

The Protein Annotated Locations Database (CropPAL) houses large scale proteomic and GFP localization data from published experimental studies in Soybean (Glycine max), Maize (Zea mays), Wheat (Triticum aestivum), Barley (Hordeum vulgare), Rice (Oryza sativa), Field mustard (Brassica rapa), Canola (Brassica napus), Sorghum (Sorghum bicolor), Potato (Solanum tuberosum), Tomato (Solanum lycopersicum), Banana (Musa acuminata) and Wine grape (Vitis vinifera) as well as precomputed predictions for protein subcellular localizations using protein sequences.