Factsheet for Bra000667.1-P

Subcellular Localization

min:

: max

Winner_takes_all: plastid

Predictor Summary:

plastid 6
mitochondrion 3
vacuole 1

Predictors

GFP

MS/MS

Papers

PPI

No PPI Data

Homology

Paralog

locus	Identity	Homology Identity

Ortholog

locus	Homology Species	Location	Identity	Homology Identity
CDX90708	Canola	plastid	96.39	92.29
AT4G09020.1	Thale cress	plastid	82.78	78.01
Bra014108.1-P	Field mustard	cytosol	20.14	70.05
PGSC0003DMT400018765	Potato	cytosol	21.81	68.56
GSMUA_Achr9P14980_001	Banana	cytosol	65.83	66.29
KRH53006	Soybean	plastid	69.86	65.16
VIT_18s0001g06520.t01	Wine grape	plastid	69.58	64.65
KRH62296	Soybean	plastid	69.44	63.86
Os09t0469400-01	Rice	plastid	29.86	63.42
KXG35819	Sorghum	plastid	67.64	62.76
TraesCS5A01G248700.1	Wheat	plastid	66.94	62.19
TraesCS5D01G255800.1	Wheat	plastid	66.81	61.98
TraesCS5B01G246400.1	Wheat	plastid	66.67	61.86
HORVU5Hr1G070160.1	Barley	plastid	66.53	61.73
Bra014111.1-P	Field mustard	mitochondrion	12.08	60.84
Solyc06g009220.2.1	Tomato	plastid	62.08	60.49
PGSC0003DMT400018766	Potato	cytosol	16.67	48.58
Bra005001.1-P	Field mustard	plastid	41.81	37.25
Bra030528.1-P	Field mustard	cytosol	30.83	30.16
Bra033388.1-P	Field mustard	plastid	31.25	26.53
Zm00001d005948_P001	Maize	golgi, plasma membrane, plastid	14.17	24.4
Bra040385.1-P	Field mustard	cytosol, plasma membrane, plastid	10.56	10.13
Bra009461.1-P	Field mustard	plastid	15.69	8.47

Protein Annotations

Gene3D:2.60.40.10	Gene3D:2.60.40.1180	MapMan:3.2.2.6	MapMan:3.2.3.3.2.1	Gene3D:3.20.20.80	EnsemblPlantsGene:Bra000667
EnsemblPlants:Bra000667.1	EnsemblPlants:Bra000667.1-P	GO:GO:0003674	GO:GO:0003824	GO:GO:0004553	GO:GO:0005975
GO:GO:0008150	GO:GO:0008152	GO:GO:0016787	InterPro:Glyco_hydro_13_N	InterPro:Glyco_hydro_13_cat_dom	InterPro:Glyco_hydro_b
InterPro:Glycoside_hydrolase_SF	InterPro:IPR013780	InterPro:IPR013783	InterPro:Ig-like_fold	InterPro:Ig_E-set	UniProt:M4C8Y9
PFAM:PF02922	PANTHER:PTHR43002	SMART:SM00642	SUPFAM:SSF51011	SUPFAM:SSF51445	SUPFAM:SSF81296
UniParc:UPI000254027F	SEG:seg	:	:	:	:

Description

AT4G09020 (E=1e-160) ATISA3, ISA3 | ISA3 (ISOAMYLASE 3); alpha-amylase/ isoamylase

Coordinates

chrA03:+:12368718..12372932

Molecular Weight (calculated)

81143.1 Da

IEP (calculated)

5.251

GRAVY (calculated)

-0.456

Length

720 amino acids

Sequence
(BLAST)

001: MLASPSYSRA ASRFPPALHS SGNVFTGFIP IRRVTGAPVC TRRSTLKVTC RRARERVIEE ESSQMAETNK QSFNVSSGEK SPLGVSQVDK GINFALFSHN
101: ATSVTLCLSL PQSDKEDDVD VVELVLDPRV NKTGDTWHIC VEDLPLRNVL YGYRVDGPGE WNQGHRFDSS ILLLDPYAKL VKGRSFFGDS SQKFAQFYGT
201: YDFETSPFDW GDDYKFPNIP EKDLVIYEMN VRAFTADESS GIDPSIAGSY LALIEKIPHL LDLGINAVEL LPVFEFDELE LQRRPNPRDH MVNTWGYSTV
301: NFFAPMSRYA SGEGDPIKAS KEFKEMVILD VVYNHTNEAD DKYPYTTSFR GIDNKIYYML DPNNQLLNYS GCGNTLNCNH PVVMELILDS LKHWVTEYHV
401: DGFRFDLASV LCRDTDGSPL SAPPLIRAIA KDSVLSRCKI IAEPWDCGGL YLVGKFPNWD RWAEWNGMYR DDVRRFIKGD CGMKGSFATR VSGSSDLYQV
501: NQRKPYHGVN FIIAHDGFTL RDLVTYNSKH NEANGEGGND GCNDNYSWNC GSEGETGDTH IKSLRVRQMK NFHLALMISQ GTPMMLMGDE YGHTRYGNNN
601: SYGHDTALNN FQWKEATRRK EGEPFQGEIT WHEDNWDNPE SKFLAFTLHD GVSGQDVYAA FNAHDYFVKA LIPLPPSGKQ WFRVADTNLE SPDDFVKEGV
701: AGVAEAYNVA PFSSILLKSM

Best Arabidopsis Sequence Match ( AT4G09020.1 )
(BLAST)

001: MLTSPSSSST YDPFSSNFSP SLTNAFSSSF TIPMGLKLSR RVTRARIFSR KIKDRSTLKV TCRRAHERVV EEEASTMTET KLFKVSSGEV SPLGVSQVDK
101: GINFALFSQN ATSVTLCLSL SQSGKDDTDD DGMIELVLDP SVNKTGDTWH ICVEDLPLNN VLYGYRVDGP GEWQQGHRFD RSILLLDPYA KLVKGHSSFG
201: DSSQKFAQFY GTYDFESSPF DWGDDYKFPN IPEKDLVIYE MNVRAFTADE SSGMDPAIGG SYLGFIEKIP HLQDLGINAV ELLPVFEFDE LELQRRSNPR
301: DHMVNTWGYS TVNFFAPMSR YASGEGDPIK ASKEFKEMVK ALHSAGIEVI LDVVYNHTNE ADDKYPYTTS FRGIDNKVYY MLDPNNQLLN FSGCGNTLNC
401: NHPVVMELIL DSLRHWVTEY HVDGFRFDLA SVLCRATDGS PLSAPPLIRA IAKDSVLSRC KIIAEPWDCG GLYLVGKFPN WDRWAEWNGM YRDDVRRFIK
501: GDSGMKGSFA TRVSGSSDLY QVNQRKPYHG VNFVIAHDGF TLRDLVSYNF KHNEANGEGG NDGCNDNHSW NCGFEGETGD AHIKSLRTRQ MKNFHLALMI
601: SQGTPMMLMG DEYGHTRYGN NNSYGHDTSL NNFQWKELDA KKQNHFRFFS EVIKFRHSHH VLKHENFLTQ GEITWHEDNW DNSESKFLAF TLHDGIGGRD
701: IYVAFNAHDY FVKALIPQPP PGKQWFRVAD TNLESPDDFV REGVAGVADT YNVAPFSSIL LQSK

Arabidopsis Description

ISA3Isoamylase 3, chloroplastic [Source:UniProtKB/Swiss-Prot;Acc:Q9M0S5]
SUBAcon: [plastid]

Hydropathy Plot

About CropPAL

The Protein Annotated Locations Database (CropPAL) houses large scale proteomic and GFP localization data from published experimental studies in Soybean (Glycine max), Maize (Zea mays), Wheat (Triticum aestivum), Barley (Hordeum vulgare), Rice (Oryza sativa), Field mustard (Brassica rapa), Canola (Brassica napus), Sorghum (Sorghum bicolor), Potato (Solanum tuberosum), Tomato (Solanum lycopersicum), Banana (Musa acuminata) and Wine grape (Vitis vinifera) as well as precomputed predictions for protein subcellular localizations using protein sequences.