Factsheet for PGSC0003DMT400018765

Subcellular Localization

min:

: max

Winner_takes_all: cytosol

Predictor Summary:

nucleus 1
cytosol 3
mitochondrion 3
plastid 1

Predictors

GFP

MS/MS

Papers

cytosol: 22313117

msms PMID: 22313117 doi

S Lim, K Chisholm, RH Coffin, RD Peters, KI Al-Mughrabi, G Wang-Pruski, DM Pinto

Department of Plant and Animal Sciences, Nova Scotia Agricultural College, Truro, Nova Scotia, Canada.

PPI

No PPI Data

Homology

Paralog

locus	Identity	Homology Identity

Ortholog

locus	Homology Species	Location	Identity	Homology Identity
Bra014111.1-P	Field mustard	mitochondrion	18.78	30.07
CDY70211	Canola	plastid	70.74	29.35
Solyc06g009220.2.1	Tomato	plastid	83.41	25.85
CDX77333	Canola	cytosol	11.35	24.76
GSMUA_Achr9P14980_001	Banana	cytosol	71.62	22.94
VIT_18s0001g06520.t01	Wine grape	plastid	75.98	22.45
Bra014108.1-P	Field mustard	cytosol	20.09	22.22
AT4G09020.1	Thale cress	plastid	73.8	22.12
CDX90708	Canola	plastid	72.49	22.07
KXG35819	Sorghum	plastid	74.67	22.04
Bra000667.1-P	Field mustard	plastid	68.56	21.81
KRH53006	Soybean	plastid	72.05	21.37
TraesCS5A01G248700.1	Wheat	plastid	72.05	21.29
HORVU5Hr1G070160.1	Barley	plastid	72.05	21.26
KRH62296	Soybean	plastid	72.49	21.2
TraesCS5B01G246400.1	Wheat	plastid	71.62	21.13
TraesCS5D01G255800.1	Wheat	plastid	71.62	21.13
CDY60217	Canola	extracellular	15.28	20.35
Os09t0469400-01	Rice	plastid	29.26	19.76
PGSC0003DMT400077770	Potato	cytosol	10.92	19.53
CDX77349	Canola	mitochondrion	17.9	10.51
CDY05042	Canola	cytosol	10.92	9.09
PGSC0003DMT400002502	Potato	plastid	33.62	8.77
PGSC0003DMT400018766	Potato	cytosol	6.55	6.07
Zm00001d005948_P001	Maize	golgi, plasma membrane, plastid	10.04	5.5
PGSC0003DMT400053345	Potato	cytosol	6.11	4.83

Protein Annotations

EnsemblPlants:PGSC0003DMT400018765	EnsemblPlantsGene:PGSC0003DMG401007274	Gene3D:3.20.20.80	GO:GO:0003674	GO:GO:0003824	GO:GO:0004553
GO:GO:0005975	GO:GO:0008150	GO:GO:0008152	GO:GO:0016787	InterPro:Glyco_hydro_13_cat_dom	InterPro:Glycoside_hydrolase_SF
PANTHER:PTHR43002	PFAM:PF00128	PGSC:PGSC0003DMG401007274	SUPFAM:SSF51445	UniParc:UPI0002963919	UniProt:M1AB40
MapMan:3.2.2.6	MapMan:3.2.3.3.2.1	:	:	:	:

Description

Isoamylase isoform 3 [Source:PGSC_GENE;Acc:PGSC0003DMG401007274]

Coordinates

chr6:-:375942..382975

Molecular Weight (calculated)

25976.4 Da

IEP (calculated)

5.136

GRAVY (calculated)

-0.440

Length

229 amino acids

Sequence
(BLAST)

001: MSRYASCGGG PVRASWEFKE MVKALHGAGI EVILDVVYNH TNEADDENPY TTSFRGIDNK VYYMVDLNNN AQLLNFAGCG NTFNCNHPTV MELILESLRH
101: WVTEYHVDGF RFDLASVLCR GTDDDSVNSE NVEKDYIENE QDATYLQAIS KDSVLSRCKI IAEPWDCGGL YLVGKFPNWD RWAEWNGKYR DDIRRFIKGD
201: AGMKGNFATR IAGSADLYRV RATASKIMH

Best Arabidopsis Sequence Match ( AT4G09020.1 )
(BLAST)

001: MLTSPSSSST YDPFSSNFSP SLTNAFSSSF TIPMGLKLSR RVTRARIFSR KIKDRSTLKV TCRRAHERVV EEEASTMTET KLFKVSSGEV SPLGVSQVDK
101: GINFALFSQN ATSVTLCLSL SQSGKDDTDD DGMIELVLDP SVNKTGDTWH ICVEDLPLNN VLYGYRVDGP GEWQQGHRFD RSILLLDPYA KLVKGHSSFG
201: DSSQKFAQFY GTYDFESSPF DWGDDYKFPN IPEKDLVIYE MNVRAFTADE SSGMDPAIGG SYLGFIEKIP HLQDLGINAV ELLPVFEFDE LELQRRSNPR
301: DHMVNTWGYS TVNFFAPMSR YASGEGDPIK ASKEFKEMVK ALHSAGIEVI LDVVYNHTNE ADDKYPYTTS FRGIDNKVYY MLDPNNQLLN FSGCGNTLNC
401: NHPVVMELIL DSLRHWVTEY HVDGFRFDLA SVLCRATDGS PLSAPPLIRA IAKDSVLSRC KIIAEPWDCG GLYLVGKFPN WDRWAEWNGM YRDDVRRFIK
501: GDSGMKGSFA TRVSGSSDLY QVNQRKPYHG VNFVIAHDGF TLRDLVSYNF KHNEANGEGG NDGCNDNHSW NCGFEGETGD AHIKSLRTRQ MKNFHLALMI
601: SQGTPMMLMG DEYGHTRYGN NNSYGHDTSL NNFQWKELDA KKQNHFRFFS EVIKFRHSHH VLKHENFLTQ GEITWHEDNW DNSESKFLAF TLHDGIGGRD
701: IYVAFNAHDY FVKALIPQPP PGKQWFRVAD TNLESPDDFV REGVAGVADT YNVAPFSSIL LQSK

Arabidopsis Description

ISA3Isoamylase 3, chloroplastic [Source:UniProtKB/Swiss-Prot;Acc:Q9M0S5]
SUBAcon: [plastid]

Hydropathy Plot

About CropPAL

The Protein Annotated Locations Database (CropPAL) houses large scale proteomic and GFP localization data from published experimental studies in Soybean (Glycine max), Maize (Zea mays), Wheat (Triticum aestivum), Barley (Hordeum vulgare), Rice (Oryza sativa), Field mustard (Brassica rapa), Canola (Brassica napus), Sorghum (Sorghum bicolor), Potato (Solanum tuberosum), Tomato (Solanum lycopersicum), Banana (Musa acuminata) and Wine grape (Vitis vinifera) as well as precomputed predictions for protein subcellular localizations using protein sequences.