Factsheet for PGSC0003DMT400018766

Subcellular Localization

min:

: max

Winner_takes_all: cytosol

Predictor Summary:

nucleus 2
cytosol 2
plastid 1
extracellular 1
mitochondrion 1

Predictors

GFP

MS/MS

Papers

PPI

No PPI Data

Homology

Paralog

locus	Identity	Homology Identity

Ortholog

locus	Homology Species	Location	Identity	Homology Identity
CDY05042	Canola	cytosol	49.8	44.73
CDX77349	Canola	mitochondrion	49.8	31.54
PGSC0003DMT400053345	Potato	cytosol	33.2	28.28
Bra014108.1-P	Field mustard	cytosol	23.48	28.02
PGSC0003DMT400077770	Potato	cytosol	10.53	20.31
GSMUA_Achr9P14980_001	Banana	cytosol	57.49	19.86
VIT_18s0001g06520.t01	Wine grape	plastid	60.73	19.35
KRH53006	Soybean	plastid	60.32	19.3
CDX77333	Canola	cytosol	8.1	19.05
KRH62296	Soybean	plastid	58.7	18.52
KXG35819	Sorghum	plastid	56.28	17.91
CDX90708	Canola	plastid	53.85	17.69
AT4G09020.1	Thale cress	plastid	54.66	17.67
HORVU5Hr1G070160.1	Barley	plastid	54.25	17.27
TraesCS5D01G255800.1	Wheat	plastid	54.25	17.27
TraesCS5A01G248700.1	Wheat	plastid	53.85	17.16
TraesCS5B01G246400.1	Wheat	plastid	53.85	17.14
Bra000667.1-P	Field mustard	plastid	48.58	16.67
Bra014111.1-P	Field mustard	mitochondrion	8.91	15.38
CDY60217	Canola	extracellular	8.1	11.63
Zm00001d005948_P001	Maize	golgi, plasma membrane, plastid	12.55	7.42
PGSC0003DMT400002502	Potato	plastid	24.7	6.95
PGSC0003DMT400018765	Potato	cytosol	6.07	6.55
CDY70211	Canola	plastid	7.29	3.26
Os09t0469400-01	Rice	plastid	2.43	1.77

Protein Annotations

KEGG:00500+2.4.1.18	Gene3D:2.60.40.1180	MapMan:3.2.2.6	MapMan:3.2.3.3.2.1	Gene3D:3.20.20.80	InterPro:A-amylase/branching_C
GO:GO:0003674	GO:GO:0003824	GO:GO:0004553	GO:GO:0005488	GO:GO:0005975	GO:GO:0008150
GO:GO:0008152	GO:GO:0016787	GO:GO:0043169	InterPro:Glyco_hydro_b	InterPro:Glycoside_hydrolase_SF	InterPro:IPR013780
UniProt:M1AB41	PFAM:PF02806	EnsemblPlantsGene:PGSC0003DMG402007274	PGSC:PGSC0003DMG402007274	EnsemblPlants:PGSC0003DMT400018766	PANTHER:PTHR43002
SUPFAM:SSF51011	SUPFAM:SSF51445	UniParc:UPI000296D44F	:	:	:

Description

Isoamylase isoform 3 [Source:PGSC_GENE;Acc:PGSC0003DMG402007274]

Coordinates

chr6:-:368214..374739

Molecular Weight (calculated)

28349.5 Da

IEP (calculated)

6.749

GRAVY (calculated)

-0.504

Length

247 amino acids

Sequence
(BLAST)

001: MKLVHHLDSD GENYSTMMRT VKVAMMDAMT TSVGIVELKE AYAIPEPKTF SNNFLPRIGE TSDANINALR SRQMKNFHLA LMVSQGTPMM LMGDEYGHTR
101: YGNNNSYGHD TAINNFQWGQ LEARKNDHFR FFSKMIKFRL SHNVLRKESF IEKNDITWLE DNWHNEESRF LAFILHDGNG GDIYLAFNAH HFSIKTAIPS
201: PPRNRSWYRV VDTNLKSPDD FVTEGVSGIS ETYDVAPYSA ILLEAKQ

Best Arabidopsis Sequence Match ( AT4G09020.1 )
(BLAST)

001: MLTSPSSSST YDPFSSNFSP SLTNAFSSSF TIPMGLKLSR RVTRARIFSR KIKDRSTLKV TCRRAHERVV EEEASTMTET KLFKVSSGEV SPLGVSQVDK
101: GINFALFSQN ATSVTLCLSL SQSGKDDTDD DGMIELVLDP SVNKTGDTWH ICVEDLPLNN VLYGYRVDGP GEWQQGHRFD RSILLLDPYA KLVKGHSSFG
201: DSSQKFAQFY GTYDFESSPF DWGDDYKFPN IPEKDLVIYE MNVRAFTADE SSGMDPAIGG SYLGFIEKIP HLQDLGINAV ELLPVFEFDE LELQRRSNPR
301: DHMVNTWGYS TVNFFAPMSR YASGEGDPIK ASKEFKEMVK ALHSAGIEVI LDVVYNHTNE ADDKYPYTTS FRGIDNKVYY MLDPNNQLLN FSGCGNTLNC
401: NHPVVMELIL DSLRHWVTEY HVDGFRFDLA SVLCRATDGS PLSAPPLIRA IAKDSVLSRC KIIAEPWDCG GLYLVGKFPN WDRWAEWNGM YRDDVRRFIK
501: GDSGMKGSFA TRVSGSSDLY QVNQRKPYHG VNFVIAHDGF TLRDLVSYNF KHNEANGEGG NDGCNDNHSW NCGFEGETGD AHIKSLRTRQ MKNFHLALMI
601: SQGTPMMLMG DEYGHTRYGN NNSYGHDTSL NNFQWKELDA KKQNHFRFFS EVIKFRHSHH VLKHENFLTQ GEITWHEDNW DNSESKFLAF TLHDGIGGRD
701: IYVAFNAHDY FVKALIPQPP PGKQWFRVAD TNLESPDDFV REGVAGVADT YNVAPFSSIL LQSK

Arabidopsis Description

ISA3Isoamylase 3, chloroplastic [Source:UniProtKB/Swiss-Prot;Acc:Q9M0S5]
SUBAcon: [plastid]

Hydropathy Plot

About CropPAL

The Protein Annotated Locations Database (CropPAL) houses large scale proteomic and GFP localization data from published experimental studies in Soybean (Glycine max), Maize (Zea mays), Wheat (Triticum aestivum), Barley (Hordeum vulgare), Rice (Oryza sativa), Field mustard (Brassica rapa), Canola (Brassica napus), Sorghum (Sorghum bicolor), Potato (Solanum tuberosum), Tomato (Solanum lycopersicum), Banana (Musa acuminata) and Wine grape (Vitis vinifera) as well as precomputed predictions for protein subcellular localizations using protein sequences.