CropPAL

Subcellular Localization

min:

: max

Winner_takes_all: endoplasmic reticulum

Predictor Summary:

nucleus 2
endoplasmic reticulum 4
extracellular 2
vacuole 2
plasma membrane 2
golgi 2

Predictors

GFP

MS/MS

Papers

Winner Takes All:	endoplasmic reticulum
Any Predictor:	nucleus, secretory
BaCelLo:	nucleus
MultiLoc:	endoplasmic reticulum
PProwler:	secretory
TargetP:	secretory
WoLF PSORT:	nucleus
YLoc:	endoplasmic reticulum

PPI

Inferred distinct locusB in Crop

locusB	locations
CDX70995
CDX88803
CDX92368
CDY09128
CDY35676

Inferred from Arabidopsis experimental PPI

Ath locusA	locusB	Ath locusB	Paper
AT3G48890.1	CDX70995	AT5G22290.1	21798944
AT3G48890.1	CDX88803	AT5G22290.1	21798944
AT3G48890.1	CDX92368	AT5G22290.1	21798944
AT3G48890.1	CDY09128	AT5G22290.1	21798944
AT3G48890.1	CDY35676	AT5G22290.1	21798944

Homology

Paralog

locus	Identity	Homology Identity

Ortholog

locus	Homology Species	Location	Identity	Homology Identity
CDY71101	Canola	endoplasmic reticulum	96.85	96.85
Bra018028.1-P	Field mustard	endoplasmic reticulum	96.4	96.4
AT3G48890.1	Thale cress	endoplasmic reticulum	71.62	68.24
CDX87002	Canola	endoplasmic reticulum	67.12	68.04
VIT_16s0100g01280.t01	Wine grape	endoplasmic reticulum	65.32	67.44
CDY37905	Canola	cytosol	36.49	61.36
PGSC0003DMT400037158	Potato	mitochondrion	61.26	59.91
Solyc03g025950.2.1	Tomato	plastid	60.81	59.47
CDY00522	Canola	endoplasmic reticulum, extracellular, golgi	57.21	58.26
CDY61583	Canola	endoplasmic reticulum, extracellular, golgi	57.21	58.26
CDY36148	Canola	nucleus	22.52	50.0
TraesCS1D01G165500.1	Wheat	golgi, plastid	48.2	43.85
TraesCS1B01G191100.2	Wheat	plastid	47.3	43.03
TraesCS1A01G173200.3	Wheat	golgi, plastid	46.4	42.21
Os10t0502500-04	Rice	plastid	49.1	41.13
EER93976	Sorghum	plastid	47.3	40.54
HORVU1Hr1G045610.5	Barley	plastid	45.95	35.66
CDY08286	Canola	golgi	21.62	19.75
CDY06492	Canola	golgi	21.62	19.67

Protein Annotations

EnsemblPlants:CDY05880	EnsemblPlantsGene:GSBRNA2T00121679001	Gene3D:3.10.120.10	GO:A0A078EV07	GO:GO:0005575	GO:GO:0016020
GO:GO:0016021	InterPro:Cyt_B5-like_heme/steroid-bd	InterPro:Cyt_B5-like_heme/steroid_sf	InterPro:IPR001199	InterPro:IPR036400	PANTHER:PTHR10281
PANTHER:PTHR10281:SF45	PFAM:PF00173	ProteinID:CDY05880	ProteinID:CDY05880.1	SEG:seg	SMART:SM01117
SUPFAM:SSF55856	TMHMM:TMhelix	UniParc:UPI0004EEDBE1	MapMan:11.3.2.1.4	:	:

Description

BnaC08g20570D

Coordinates

chrLK031966:+:259403..260943

Molecular Weight (calculated)

24424.4 Da

IEP (calculated)

4.154

GRAVY (calculated)

-0.525

Length

222 amino acids

Sequence
(BLAST)

001: MVVEVWETVK ETITAYTGLS PAAFFTVLAL AFAVYQVVSG FFVSPEEHRP RSTEDYPEPE PLPPPVQLGE ITEEELKQYD GSDSKKPLLM AIKGQIYDVS
101: QSRMFYGPAG PYALFAGKDA SRALAKMSFE ETDLTGDISG LGPFELDALQ DWEYKFMSKY VKVGTIQKND GQGKESAEPS TNTVEEASPE VAHGETSRSI
201: EEKTEETTEK KDVANGDAAK ED

Best Arabidopsis Sequence Match ( AT3G48890.1 )
(BLAST)

001: MVQQIWETLK ETITAYTGLS PAAFFTVLAL AFAVYQVVSG FFVSPEVHRP RSLEVQPQSE PLPPPVQLGE ITEEELKLYD GSDSKKPLLM AIKGQIYDVS
101: QSRMFYGPGG PYALFAGKDA SRALAKMSFE DQDLTGDISG LGAFELEALQ DWEYKFMSKY VKVGTIQKKD GEGKESSEPS EAKTASAEGL STNTGEEASA
201: ITHDETSRST GEKIAETTEK KDVATDDDDA AKE

Arabidopsis Description

MSBP2Membrane steroid-binding protein 2 [Source:UniProtKB/Swiss-Prot;Acc:Q9M2Z4]
SUBAcon: [endoplasmic reticulum]

Hydropathy Plot

About CropPAL

The Protein Annotated Locations Database (CropPAL) houses large scale proteomic and GFP localization data from published experimental studies in Soybean (Glycine max), Maize (Zea mays), Wheat (Triticum aestivum), Barley (Hordeum vulgare), Rice (Oryza sativa), Field mustard (Brassica rapa), Canola (Brassica napus), Sorghum (Sorghum bicolor), Potato (Solanum tuberosum), Tomato (Solanum lycopersicum), Banana (Musa acuminata) and Wine grape (Vitis vinifera) as well as precomputed predictions for protein subcellular localizations using protein sequences.