CropPAL

Subcellular Localization

min:

: max

Winner_takes_all: plastid, cytosol

Predictor Summary:

nucleus 3
plastid 4
cytosol 3

Predictors

GFP

MS/MS

Papers

Winner Takes All:	cytosol, plastid
Any Predictor:	cytosol, nucleus, plastid
BaCelLo:	nucleus
ChloroP:	plastid
EpiLoc:	cytosol
iPSORT:	plastid
MultiLoc:	cytosol
Plant-mPloc:	nucleus
PProwler:	plastid
TargetP:	plastid
WoLF PSORT:	nucleus
YLoc:	cytosol

PPI

Inferred distinct locusB in Crop

locusB	locations
EER87882
EER94589
EER96589
EER96590
EES03370
EES18716
OQU80381

Inferred from Arabidopsis experimental PPI

Ath locusA	locusB	Ath locusB	Paper
AT4G08480.1	EER94589	AT4G01370.1	22643122
AT4G08500.1	EER87882	AT4G23810.1	17587183
AT4G08500.1	EER96589	AT4G23810.1	17587183
AT4G08500.1	EER96590	AT4G23810.1	17587183
AT4G08500.1	EES03370	AT4G23810.1	17587183
AT4G08500.1	EES18716	AT4G23810.1	17587183
AT4G08500.1	OQU80381	AT4G23810.1	17587183

Homology

Paralog

locus	Identity	Homology Identity

Ortholog

locus	Homology Species	Location	Identity	Homology Identity
Zm00001d028514_P002	Maize	plastid	90.73	83.92
Zm00001d047875_P003	Maize	plastid	85.26	80.09
Os03t0262200-00	Rice	cytosol, plastid	76.66	77.55
TraesCS4D01G217000.1	Wheat	plastid	74.34	75.84
TraesCS4B01G216700.1	Wheat	cytosol, plastid	74.17	75.8
TraesCS4A01G087700.1	Wheat	cytosol, plastid	74.67	75.29
OQU91136	Sorghum	cytosol	42.22	68.73
PGSC0003DMT400067237	Potato	cytosol	32.28	67.01
HORVU4Hr1G061550.1	Barley	cytosol, plastid	75.17	65.7
Bra039693.1-P	Field mustard	cytosol	27.98	61.68
GSMUA_Achr11P...	Banana	cytosol	54.47	60.37
GSMUA_Achr10P...	Banana	cytosol	51.99	59.81
GSMUA_Achr7P11850_001	Banana	mitochondrion, nucleus	52.65	57.4
GSMUA_Achr2P07690_001	Banana	nucleus	54.3	56.55
VIT_12s0142g00700.t01	Wine grape	cytosol	24.67	56.44
CDY36607	Canola	cytosol	30.63	56.23
GSMUA_Achr1P12210_001	Banana	mitochondrion	53.64	56.06
KRH10459	Soybean	cytosol	40.56	55.68
CDY24072	Canola	cytosol	32.28	54.78
CDY18020	Canola	cytosol	6.95	53.85
VIT_12s0034g00750.t01	Wine grape	cytosol	26.82	51.76
KRH53173	Soybean	nucleus	45.2	49.19
VIT_12s0034g00240.t01	Wine grape	nucleus	34.27	49.05
VIT_18s0001g03960.t01	Wine grape	mitochondrion, nucleus	45.53	48.5
KRH42244	Soybean	cytosol	37.58	48.3
KRH16614	Soybean	mitochondrion	44.04	48.1
KRH18819	Soybean	nucleus	47.19	47.98
KRH16616	Soybean	nucleus	46.69	47.8
KRH64744	Soybean	nucleus	44.7	47.7
KRH58364	Soybean	cytosol, plastid	38.91	47.0
Bra000647.1-P	Field mustard	cytosol	41.39	46.21
Solyc07g053170.2.1	Tomato	cytosol	45.7	45.92
CDY34000	Canola	cytosol	41.72	45.82
OQU85800	Sorghum	mitochondrion	39.24	45.66
CDY69517	Canola	cytosol	41.39	45.54
CDY36198	Canola	cytosol	42.55	44.7
KXG20217	Sorghum	cytosol	46.69	43.19
Bra037860.1-P	Field mustard	cytosol	42.72	43.07
CDX75154	Canola	cytosol	43.05	42.98
AT4G08500.1	Thale cress	cytosol, mitochondrion	42.05	41.78
PGSC0003DMT400061014	Potato	mitochondrion	42.72	38.74
Solyc01g104530.2.1	Tomato	nucleus	42.38	38.5
KRH10458	Soybean	cytosol	29.97	38.35
AT4G08470.1	Thale cress	cytosol	32.62	35.18
CDY18021	Canola	nucleus	21.19	31.22
AT4G08480.1	Thale cress	cytosol, plastid	39.57	30.92
EES03486	Sorghum	mitochondrion	15.73	26.91
OQU85116	Sorghum	cytosol	23.18	25.32
OQU92769	Sorghum	mitochondrion	15.89	21.52
EES01324	Sorghum	plastid	16.39	21.29
KXG28265	Sorghum	mitochondrion	23.51	21.26
EER90833	Sorghum	mitochondrion, plastid	25.66	20.89
OQU78383	Sorghum	mitochondrion	15.07	20.31
OQU81618	Sorghum	mitochondrion	24.01	19.62
OQU87391	Sorghum	mitochondrion	14.74	19.6
OQU88348	Sorghum	plastid	23.84	19.05
OQU87390	Sorghum	plastid	16.23	18.92
EES05759	Sorghum	cytosol	27.98	18.88
EER98846	Sorghum	nucleus	20.86	18.56
OQU82130	Sorghum	cytosol, plastid	27.15	18.3
KXG25169	Sorghum	nucleus	20.86	18.13
KXG22458	Sorghum	mitochondrion	14.07	17.49
EES13018	Sorghum	cytosol	18.87	8.53

Protein Annotations

Gene3D:1.10.510.10	MapMan:18.4.2.2	Gene3D:3.30.200.20	EntrezGene:8054301	UniProt:C5WQ75	EnsemblPlants:EER92515
ProteinID:EER92515	ProteinID:EER92515.1	GO:GO:0000166	GO:GO:0003674	GO:GO:0003824	GO:GO:0004672
GO:GO:0004674	GO:GO:0005488	GO:GO:0005524	GO:GO:0005575	GO:GO:0005622	GO:GO:0005623
GO:GO:0005737	GO:GO:0006464	GO:GO:0006468	GO:GO:0006950	GO:GO:0007049	GO:GO:0007154
GO:GO:0007165	GO:GO:0007346	GO:GO:0008150	GO:GO:0008152	GO:GO:0009987	GO:GO:0016301
GO:GO:0016740	GO:GO:0019538	GO:GO:0023014	GO:GO:0031098	GO:GO:0032147	InterPro:IPR000719
InterPro:Kinase-like_dom_sf	PFAM:PF00069	ScanProsite:PS00107	ScanProsite:PS00108	PFscan:PS50011	PANTHER:PTHR24361
PANTHER:PTHR24361:SF404	InterPro:Prot_kinase_dom	InterPro:Protein_kinase_ATP_BS	SMART:SM00220	EnsemblPlantsGene:SORBI_3001G429300	SUPFAM:SSF56112
unigene:Sbi.1900	InterPro:Ser/Thr_kinase_AS	UniParc:UPI0001A82647	RefSeq:XP_002465517.1	SEG:seg	:

Description

hypothetical protein

Coordinates

chr1:+:70839331..70844674

Molecular Weight (calculated)

65293.3 Da

IEP (calculated)

6.307

GRAVY (calculated)

-0.495

Length

604 amino acids

Sequence
(BLAST)

001: MGPPHAAQDP VPSPSPSDGS GSGSSRRRLR RLDRRNASKH IGYTAPNYCQ YPPSPQPAAS APGSGPASLA ASVACSLDLV NSFRIGGSGD GGGDLRFLCE
101: SLGLSGPDDF AIPLADWEAH KAVRSSASAS SSPSSARPNH DSPQRDSPLC QVGAEEPAQA ADADPELPAA TGRDGPIEAP ERPARLDPPP ESTCPDARRA
201: AGEGGIKGVR PPPVLKPPPS MALPAVCGVG STWDILRSFA PDEKEDAPAS RSDRRFGHRD AVEKDDDENA AVLLNLDDLG LEESSEGFTG TSSLSTTNDD
301: ETSSTTTESM FYISPNGRFR RRIRSWSRGV LLGSGSFGTV YEGISDEGVF FAVKEVNLFD QGSNAKQCII QLEQEIALLS QFEHENIVQY YGTDKEDSKL
401: YIFLELVTQG SLASLYQKYR LRDTHVSAYT RQILNGLIYL HERNIVHRDI KCANILVHAN GSVKLADFGL AKEITKFNAV KSCKGTVYWM APEVVNPKKT
501: YGPAADIWSL GCTVLEMLTR RIPYPDLEWT QALYRIGKGE SPAIPNALSK DARDFISQCV KSNPEDRPSA SKLLEHPFVN KSIRSVRSMR TISSRSNSST
601: RGIN

Best Arabidopsis Sequence Match ( AT4G08500.1 )
(BLAST)

001: MDRILARMKK STGRRGGDKN ITPVRRLERR DAARNINYDA ASCSSSSAED LSVSTSSLMT RSLEFPEPTS FRIGGGVGEM DRIYRSLGVS GPDDLAISFD
101: AWEACKKRSS SDVVNRFKSF DLDKVRDQDL SEEGPSGVVV GSDSMNHKVQ GQDLSEAGPS GGIVTELSEI GNLITPVDRL VADGVVENRR VMERTPTIVK
201: SKGYLVPNNV VAVGVGVGGG IKGLRPPVLK PPPAMKRPPI DHRGSSWDFL THFAPSETVK RPSSSSSSSE DGCDEEEGKE EEAEAEEMGA RFIQLGDTAD
301: ETCSFTTNEG DSSSTVSNTS PIYPDGGAII TSWQKGQLLG RGSFGSVYEG ISGDGDFFAV KEVSLLDQGS QAQECIQQLE GEIKLLSQLQ HQNIVRYRGT
401: AKDGSNLYIF LELVTQGSLL KLYQRYQLRD SVVSLYTRQI LDGLKYLHDK GFIHRDIKCA NILVDANGAV KLADFGLAKV SKFNDIKSCK GTPFWMAPEV
501: INRKDSDGYG SPADIWSLGC TVLEMCTGQI PYSDLEPVQA LFRIGRGTLP EVPDTLSLDA RLFILKCLKV NPEERPTAAE LLNHPFVRRP LPSVGSGGSG
601: SASPLLRR

Arabidopsis Description

MEKK1Mitogen-activated protein kinase kinase kinase 1 [Source:UniProtKB/Swiss-Prot;Acc:Q39008]
SUBAcon: [mitochondrion,cytosol]

Hydropathy Plot

About CropPAL

The Protein Annotated Locations Database (CropPAL) houses large scale proteomic and GFP localization data from published experimental studies in Soybean (Glycine max), Maize (Zea mays), Wheat (Triticum aestivum), Barley (Hordeum vulgare), Rice (Oryza sativa), Field mustard (Brassica rapa), Canola (Brassica napus), Sorghum (Sorghum bicolor), Potato (Solanum tuberosum), Tomato (Solanum lycopersicum), Banana (Musa acuminata) and Wine grape (Vitis vinifera) as well as precomputed predictions for protein subcellular localizations using protein sequences.