CropPAL

Subcellular Localization

min:

: max

Winner_takes_all: cytosol

Predictor Summary:

nucleus 2
cytosol 2
mitochondrion 1

Predictors

GFP

MS/MS

Papers

Winner Takes All:	cytosol
Any Predictor:	cytosol, nucleus
BaCelLo:	nucleus
MultiLoc:	cytosol
PProwler:	mitochondrion
WoLF PSORT:	nucleus
YLoc:	cytosol

PPI

Inferred distinct locusB in Crop

locusB	locations
EER87882
EER94589
EER96589
EER96590
EES03370
EES18716
OQU80381

Inferred from Arabidopsis experimental PPI

Ath locusA	locusB	Ath locusB	Paper
AT4G08480.1	EER94589	AT4G01370.1	22643122
AT4G08500.1	EER87882	AT4G23810.1	17587183
AT4G08500.1	EER96589	AT4G23810.1	17587183
AT4G08500.1	EER96590	AT4G23810.1	17587183
AT4G08500.1	EES03370	AT4G23810.1	17587183
AT4G08500.1	EES18716	AT4G23810.1	17587183
AT4G08500.1	OQU80381	AT4G23810.1	17587183

Homology

Paralog

locus	Identity	Homology Identity

Ortholog

locus	Homology Species	Location	Identity	Homology Identity
PGSC0003DMT400067237	Potato	cytosol	27.26	61.17
Bra039693.1-P	Field mustard	cytosol	25.42	60.58
OQU91136	Sorghum	cytosol	34.3	60.38
VIT_12s0142g00700.t01	Wine grape	cytosol	22.36	55.3
CDY36607	Canola	cytosol	26.95	53.5
CDY18020	Canola	cytosol	6.28	52.56
CDY24072	Canola	cytosol	28.33	51.97
VIT_12s0034g00750.t01	Wine grape	cytosol	23.89	49.84
GSMUA_Achr10P...	Banana	cytosol	38.74	48.19
GSMUA_Achr11P...	Banana	cytosol	39.66	47.52
KRH10459	Soybean	cytosol	31.55	46.82
EER92515	Sorghum	cytosol, plastid	43.19	46.69
TraesCS4B01G216700.1	Wheat	cytosol, plastid	41.5	45.85
TraesCS4D01G217000.1	Wheat	plastid	41.5	45.78
GSMUA_Achr7P11850_001	Banana	mitochondrion, nucleus	38.74	45.67
TraesCS4A01G087700.1	Wheat	cytosol, plastid	41.5	45.24
GSMUA_Achr1P12210_001	Banana	mitochondrion	39.97	45.16
Os03t0262200-00	Rice	cytosol, plastid	41.19	45.06
GSMUA_Achr2P07690_001	Banana	nucleus	39.36	44.31
VIT_12s0034g00240.t01	Wine grape	nucleus	28.02	43.36
KRH42244	Soybean	cytosol	30.32	42.13
OQU85800	Sorghum	mitochondrion	32.31	40.66
KRH58364	Soybean	cytosol, plastid	31.09	40.6
Bra000647.1-P	Field mustard	cytosol	33.38	40.3
KRH53173	Soybean	nucleus	34.15	40.18
CDY34000	Canola	cytosol	33.54	39.82
KRH64744	Soybean	nucleus	34.46	39.75
CDY69517	Canola	cytosol	33.38	39.71
VIT_18s0001g03960.t01	Wine grape	mitochondrion, nucleus	34.3	39.51
HORVU4Hr1G061550.1	Barley	cytosol, plastid	41.5	39.22
KRH16614	Soybean	mitochondrion	32.31	38.16
KRH16616	Soybean	nucleus	34.3	37.97
CDY36198	Canola	cytosol	33.08	37.57
KRH18819	Soybean	nucleus	34.0	37.37
Solyc07g053170.2.1	Tomato	cytosol	33.84	36.77
Bra037860.1-P	Field mustard	cytosol	33.08	36.06
CDX75154	Canola	cytosol	33.38	36.03
AT4G08500.1	Thale cress	cytosol, mitochondrion	33.08	35.53
KRH10458	Soybean	cytosol	24.2	33.47
AT4G08470.1	Thale cress	cytosol	28.33	33.04
PGSC0003DMT400061014	Potato	mitochondrion	33.54	32.88
Solyc01g104530.2.1	Tomato	nucleus	33.38	32.78
AT4G08480.1	Thale cress	cytosol, plastid	31.24	26.39
CDY18021	Canola	nucleus	16.08	25.61
EES03486	Sorghum	mitochondrion	13.78	25.5
OQU85116	Sorghum	cytosol	19.45	22.97
OQU92769	Sorghum	mitochondrion	14.24	20.85
EES01324	Sorghum	plastid	14.7	20.65
KXG28265	Sorghum	mitochondrion	20.52	20.06
OQU78383	Sorghum	mitochondrion	13.63	19.87
OQU87391	Sorghum	mitochondrion	13.48	19.38
EER90833	Sorghum	mitochondrion, plastid	21.13	18.6
EER98846	Sorghum	nucleus	19.14	18.41
OQU87390	Sorghum	plastid	14.4	18.15
OQU81618	Sorghum	mitochondrion	20.37	18.0
OQU88348	Sorghum	plastid	20.83	17.99
KXG25169	Sorghum	nucleus	18.38	17.27
KXG22458	Sorghum	mitochondrion	12.4	16.67
EES05759	Sorghum	cytosol	22.82	16.65
OQU82130	Sorghum	cytosol, plastid	22.82	16.63
EES13018	Sorghum	cytosol	17.0	8.3

Protein Annotations

Gene3D:1.10.510.10	MapMan:18.4.1.46	MapMan:18.4.2.2	Gene3D:3.30.200.20	UniProt:A0A194YJT7	GO:GO:0000166
GO:GO:0003674	GO:GO:0003824	GO:GO:0004672	GO:GO:0005488	GO:GO:0005524	GO:GO:0006464
GO:GO:0006468	GO:GO:0008150	GO:GO:0008152	GO:GO:0009987	GO:GO:0016301	GO:GO:0016740
GO:GO:0019538	InterPro:IPR000719	EnsemblPlants:KXG20217	ProteinID:KXG20217	ProteinID:KXG20217.1	ProteinID:KXG20218.1
InterPro:Kinase-like_dom_sf	PFAM:PF00069	PIRSF:PIRSF000654	ScanProsite:PS00107	ScanProsite:PS00108	PFscan:PS50011
PANTHER:PTHR24361	PANTHER:PTHR24361:SF404	InterPro:Prot_kinase_dom	InterPro:Protein_kinase_ATP_BS	SMART:SM00220	EnsemblPlantsGene:SORBI_3010G171600
SUPFAM:SSF56112	InterPro:Ser/Thr_kinase_AS	UniParc:UPI0007F190F7	SEG:seg	:	:

Description

hypothetical protein

Coordinates

chr10:+:50370481..50383361

Molecular Weight (calculated)

74221.8 Da

IEP (calculated)

6.029

GRAVY (calculated)

-0.335

Length

653 amino acids

Sequence
(BLAST)

001: MDIEFSSCDM LESMLQYGSS GPRNLSLEYL RNITNNFSDE RLLGEGGFGT VYKGVLPNGE SMAVKKLNSS MPGVKDKQFE NEAYHLMRLK HPNIVLLVGW
101: CSETEDIYVE YNGKYICAEK SERLLCLEYM PKGNLRGYLS DESSGLTWET RYKIIEGICY GLHYLHEERQ LNAPIIHMDL KPANILLDNN MVPKIADFGM
201: SRLFSEEKTW TCTISRDGTL GYMAPEYINR GLISTKSDIF SLGVIIIEIV TGQRDYPDES ETGISSQEFT ELVLKNWRDR LQKSLRYTSL EIDYQQIKRC
301: IEIGLDCLKL DRWRRPTISQ VIKMLHGTEC VDFSGRKEVM SLTNEDSCSI LHQDIRLGQS SDNCTDTSST TTEFMFYTSP NGRSSRKKIR SWTRGALLGS
401: GSFGMVYEAI TDEGVFFAVK EVSLFEGIHA KLCIFQLEQE IELLSQFEHE NIVQYYGTDK EDSKLYVFLE LMTQGSLASL SQKYHLQDNH VSTYTRQILN
501: GLTYLHERNI VHRDIKCANI LVHANGSVKL ADFGLAKELS KFSAAKSCKG TVYWMAPEVV NPKKTYGPAA DIWSLGCTVL EMLTRQVPYP DMEWTQALFR
601: IGKGEAPSIP SALSKDARDF ISQCVKSNPE DRPSASELLK HPFLINRSMR SPI

Best Arabidopsis Sequence Match ( AT4G08500.1 )
(BLAST)

001: MDRILARMKK STGRRGGDKN ITPVRRLERR DAARNINYDA ASCSSSSAED LSVSTSSLMT RSLEFPEPTS FRIGGGVGEM DRIYRSLGVS GPDDLAISFD
101: AWEACKKRSS SDVVNRFKSF DLDKVRDQDL SEEGPSGVVV GSDSMNHKVQ GQDLSEAGPS GGIVTELSEI GNLITPVDRL VADGVVENRR VMERTPTIVK
201: SKGYLVPNNV VAVGVGVGGG IKGLRPPVLK PPPAMKRPPI DHRGSSWDFL THFAPSETVK RPSSSSSSSE DGCDEEEGKE EEAEAEEMGA RFIQLGDTAD
301: ETCSFTTNEG DSSSTVSNTS PIYPDGGAII TSWQKGQLLG RGSFGSVYEG ISGDGDFFAV KEVSLLDQGS QAQECIQQLE GEIKLLSQLQ HQNIVRYRGT
401: AKDGSNLYIF LELVTQGSLL KLYQRYQLRD SVVSLYTRQI LDGLKYLHDK GFIHRDIKCA NILVDANGAV KLADFGLAKV SKFNDIKSCK GTPFWMAPEV
501: INRKDSDGYG SPADIWSLGC TVLEMCTGQI PYSDLEPVQA LFRIGRGTLP EVPDTLSLDA RLFILKCLKV NPEERPTAAE LLNHPFVRRP LPSVGSGGSG
601: SASPLLRR

Arabidopsis Description

MEKK1Mitogen-activated protein kinase kinase kinase 1 [Source:UniProtKB/Swiss-Prot;Acc:Q39008]
SUBAcon: [mitochondrion,cytosol]

Hydropathy Plot

About CropPAL

The Protein Annotated Locations Database (CropPAL) houses large scale proteomic and GFP localization data from published experimental studies in Soybean (Glycine max), Maize (Zea mays), Wheat (Triticum aestivum), Barley (Hordeum vulgare), Rice (Oryza sativa), Field mustard (Brassica rapa), Canola (Brassica napus), Sorghum (Sorghum bicolor), Potato (Solanum tuberosum), Tomato (Solanum lycopersicum), Banana (Musa acuminata) and Wine grape (Vitis vinifera) as well as precomputed predictions for protein subcellular localizations using protein sequences.