CropPAL

Subcellular Localization

min:

: max

Winner_takes_all: nucleus

Predictor Summary:

cytosol 5
mitochondrion 2
extracellular 1
endoplasmic reticulum 1
vacuole 1
plasma membrane 1
golgi 1
plastid 1

Predictors

GFP

MS/MS

Papers

Winner Takes All:	nucleus
Any Predictor:	cytosol, mitochondrion, plastid, secretory
BaCelLo:	cytosol
EpiLoc:	cytosol
MultiLoc:	cytosol
Plant-mPloc:	cytosol, mitochondrion
PProwler:	mitochondrion
TargetP:	secretory
WoLF PSORT:	plastid
YLoc:	cytosol

nucleus: 21132161
endoplasmic reticulum: 27224218
nucleus: 27291164

msms PMID: 21132161 doi

B Cooper, KB Campbell, J Feng, WM Garrett, R Frederick

Soybean Genomics and Improvement Laboratory, USDA-ARS, Beltsville, MD 20705, USA. bret.cooper@ars.usda.gov

msms PMID: 27291164 doi

X Yin, S Komatsu

Graduate School of Life and Environmental Sciences, University of Tsukuba , Tsukuba 305-8572, Japan., National Institute of Crop Science, National Agriculture and Food Research Organization , Tsukuba 305-8518, Japan.

msms PMID: 27224218 doi

X Wang, S Komatsu

PPI

No PPI Data

Homology

Paralog

locus	Identity	Homology Identity

Ortholog

locus	Homology Species	Location	Identity	Homology Identity
KRH36263	Soybean	cytosol, endoplasmic reticulum, nucleus	97.64	97.64
VIT_06s0004g01160.t01	Wine grape	cytosol	90.09	89.95
Solyc01g006280.2.1	Tomato	extracellular, nucleus	88.21	88.49
PGSC0003DMT400081881	Potato	cytosol	88.05	88.33
GSMUA_Achr10P...	Banana	cytosol, mitochondrion, plastid	81.76	87.69
CDY37133	Canola	cytosol	86.79	87.2
Bra018851.1-P	Field mustard	cytosol	86.79	87.2
CDY22798	Canola	cytosol	86.48	86.89
OQU89595	Sorghum	cytosol	86.48	86.61
Bra030488.1-P	Field mustard	cytosol	85.85	86.39
CDY29587	Canola	cytosol	85.53	86.08
CDY62106	Canola	cytosol	54.09	85.57
KRH33286	Soybean	nucleus	48.11	79.27
Os09t0446800-01	Rice	plastid	86.01	77.37
TraesCS5A01G232800.1	Wheat	nucleus	86.32	77.32
TraesCS5B01G231100.1	Wheat	plastid	86.32	76.68
TraesCS5D01G235400.2	Wheat	plastid	85.53	76.08
Zm00001d020669_P001	Maize	extracellular	86.16	74.76
HORVU5Hr1G058370.1	Barley	cytosol	44.03	74.47
CDY62107	Canola	plastid	27.67	72.43
AT2G12280.1	Thale cress	plastid	9.91	70.0
AT2G12200.1	Thale cress	mitochondrion, plastid	6.6	66.67

Protein Annotations

KEGG:00670+6.3.4.3	KEGG:00720+6.3.4.3	Gene3D:1.10.8.770	EntrezGene:100806844	Gene3D:3.10.410.10	MapMan:7.5.7.1
EMBL:ACUP02012778	InterPro:Formate_THF_ligase	InterPro:Formate_THF_ligase_CS	EnsemblPlantsGene:GLYMA_20G243700	GO:GO:0000166	GO:GO:0003674
GO:GO:0003824	GO:GO:0004329	GO:GO:0004477	GO:GO:0004488	GO:GO:0005488	GO:GO:0005524
GO:GO:0005575	GO:GO:0005622	GO:GO:0005623	GO:GO:0005737	GO:GO:0006139	GO:GO:0008150
GO:GO:0008152	GO:GO:0009058	GO:GO:0009113	GO:GO:0009257	GO:GO:0009987	GO:GO:0016787
GO:GO:0055114	UniProt:I1NJ85	EnsemblPlants:KRG93013	ProteinID:KRG93013	ProteinID:KRG93013.1	HAMAP:MF_01543
InterPro:P-loop_NTPase	PFAM:PF01268	ScanProsite:PS00721	ScanProsite:PS00722	PANTHER:PTHR43274	MetaCyc:PWY-1722
MetaCyc:PWY-2161	MetaCyc:PWY-2201	MetaCyc:PWY-3841	SUPFAM:SSF52540	UniParc:UPI000233DE2D	SEG:seg

Description

hypothetical protein

Coordinates

chr20:-:47396292..47400237

Molecular Weight (calculated)

67441.4 Da

IEP (calculated)

6.975

GRAVY (calculated)

0.033

Length

636 amino acids

Sequence
(BLAST)

001: MSSSTTVRKL QVVSPVPADI DIANSVEPVH ISQIAKDLNL SPNHYDLYGK YKAKVLLSVL DELQGSEDGY YVVVGGITPT PLGEGKSTTT VGLCQALGAF
101: LDKKVVTCLR QPSQGPTFGI KGGAAGGGYS QVIPMDEFNL HLTGDIHAIT AANNLLAAAI DTRIFHESTQ SDKALFNRLC PPNKEGKRSF SDVMFRRLTK
201: LGISKTNPDD LTPEEVNKFA RLDIDPNSIT WRRVMDINDR FLRKIAIGQG PDEKGMVRET GFDISVASEI MAVLALTTSL ADMRERLGKM VIGNSKSGDP
301: VTADDLGVGG ALTVLMKDAI HPTLMQTLEG TPVLVHAGPF ANIAHGNSSI VADKIALKLV GPGGFVVTEA GFGADIGAEK FMNIKCRYSG LTPQCAIIVA
401: TIRALKMHGG GPAVVAGRPL DHAYLTENVA LVEAGCVNMA RHISNTKSYG VNVVVAINKF STDTEAELNA VRSAALAAGA YDAVICTHHA NGGKGAVDLG
501: IAVQKACENV TQPLKFLYPV DLSIKEKIEA IAKSYGASGV EYSEQAEKQI EMYSKQGFSG LPICMAKTQY SFSDNAAAKG APSGFVLPIR DVRASIGAGF
601: IYPLVGTMST MPGLPTRPCF YDIDLDTTTG KVIGLS

Best Arabidopsis Sequence Match ( AT1G50480.1 )
(BLAST)

001: MSSSTRKLEV VSPVPADIDI ANSVEPLHIS EIAKDLNINP LHYDLYGKYK AKVLLSAFDE LQGQEDGYYV VVGGITPTPL GEGKSTTTVG LCQALGAYLD
101: KKVVTCLRQP SQGPTFGIKG GAAGGGYSQV IPMDEFNLHL TGDIHAITAS NNLLAAAIDT RIFHETSQSD KALFNRLCPP NKEGKRSFSD IMFRRLTKLG
201: ISKTSPEELT PEEIKKFARL DIDPASITWR RVMDVNDRFL RKITIGQGPE EKGMTRETGF DISVASEIMA VLALTTSLGD MRERLGKMVI GNSKAGDPIT
301: ADDLGVGGAL TVLMKDAINP TLMQTLEGTP VLVHAGPFAN IAHGNSSIVA DKIALKLVGP GGFVVTEAGF GSDIGTEKFM NIKCRYSGLT PQCAIVVATV
401: RALKMHGGGP DVVAGRPLDR AYVSENVSLV EAGCVNLAKH ISNTKAYGVN VIVAVNMFAT DTEAELNAVR KFSMDAGAFD AVVCSHHAHS GKGAVDLGIA
501: VEKACQNITQ PLRFLYPLDI GIKDKIEAIA KSYGASGVEY SDQAEKQIEM YTQQGFSNLP ICMSKTQYSF SHDASKKGAP SGFVLPIRDV RGSIGAGFIY
601: PLVGTMSTMP GLPTRPCFYE IDIDTETGKV RGLS

Arabidopsis Description

THFSFormate--tetrahydrofolate ligase [Source:UniProtKB/Swiss-Prot;Acc:Q9SPK5]
SUBAcon: [cytosol]

Hydropathy Plot

About CropPAL

The Protein Annotated Locations Database (CropPAL) houses large scale proteomic and GFP localization data from published experimental studies in Soybean (Glycine max), Maize (Zea mays), Wheat (Triticum aestivum), Barley (Hordeum vulgare), Rice (Oryza sativa), Field mustard (Brassica rapa), Canola (Brassica napus), Sorghum (Sorghum bicolor), Potato (Solanum tuberosum), Tomato (Solanum lycopersicum), Banana (Musa acuminata) and Wine grape (Vitis vinifera) as well as precomputed predictions for protein subcellular localizations using protein sequences.