CropPAL

Subcellular Localization

min:

: max

Winner_takes_all: nucleus

Predictor Summary:

cytosol 3
extracellular 4
endoplasmic reticulum 5
vacuole 5
plasma membrane 4
golgi 4
plastid 1

Predictors

GFP

MS/MS

Papers

Winner Takes All:	nucleus
Any Predictor:	cytosol, plastid, secretory
BaCelLo:	cytosol
EpiLoc:	cytosol
iPSORT:	secretory
MultiLoc:	cytosol
Plant-mPloc:	plastid
Predotar:	secretory
PProwler:	secretory
TargetP:	secretory
WoLF PSORT:	endoplasmic reticulum
YLoc:	vacuole

nucleus: 21132161
nucleus: 28499913

msms PMID: 21132161 doi

B Cooper, KB Campbell, J Feng, WM Garrett, R Frederick

Soybean Genomics and Improvement Laboratory, USDA-ARS, Beltsville, MD 20705, USA. bret.cooper@ars.usda.gov

msms PMID: 28499913 doi

S Komatsu, X Wang, X Yin, Y Nanjo, H Ohyanagi, K Sakata

Department of Life Science and Informatics, Maebashi Institute of Technology, Maebashi 371-0816, Japan. Electronic address: ksakata@maebashi-it.ac.jp., Institute of Crop Science, National Agriculture and Food Research Organization, Tsukuba 305-8518, Japan., Institute of Crop Science, National Agriculture and Food Research Organization, Tsukuba 305-8518, Japan; Faculty of Life and Environmental Sciences, University of Tsukuba, Tsukuba 305-8572, Japan., Institute of Crop Science, National Agriculture and Food Research Organization, Tsukuba 305-8518, Japan; Faculty of Life and Environmental Sciences, University of Tsukuba, Tsukuba 305-8572, Japan. Electronic address: komatsu.setsuko.fu@u.tsukuba.ac.jp., King Abdullah University of Science and Technology (KAUST), Computational Bioscience Research Center (CBRC), Thuwal 23955-6900, Saudi Arabia.

PPI

No PPI Data

Homology

Paralog

locus	Identity	Homology Identity

Ortholog

locus	Homology Species	Location	Identity	Homology Identity
CDY62107	Canola	plastid	45.6	72.43
KRG93013	Soybean	nucleus	79.27	48.11
KRH36263	Soybean	cytosol, endoplasmic reticulum, nucleus	77.98	47.33
HORVU5Hr1G058370.1	Barley	cytosol	45.08	46.28
VIT_06s0004g01160.t01	Wine grape	cytosol	72.54	43.96
Solyc01g006280.2.1	Tomato	extracellular, nucleus	71.24	43.38
PGSC0003DMT400081881	Potato	cytosol	70.73	43.06
Bra018851.1-P	Field mustard	cytosol	70.21	42.81
CDY37133	Canola	cytosol	70.21	42.81
Bra030488.1-P	Field mustard	cytosol	69.69	42.56
CDY22798	Canola	cytosol	69.69	42.5
CDY29587	Canola	cytosol	69.17	42.25
OQU89595	Sorghum	cytosol	68.91	41.89
GSMUA_Achr10P...	Banana	cytosol, mitochondrion, plastid	61.14	39.8
TraesCS5A01G232800.1	Wheat	nucleus	69.95	38.03
Os09t0446800-01	Rice	plastid	69.43	37.91
TraesCS5D01G235400.2	Wheat	plastid	70.21	37.9
TraesCS5B01G231100.1	Wheat	plastid	70.21	37.85
Zm00001d020669_P001	Maize	extracellular	69.43	36.56
CDY62106	Canola	cytosol	16.84	16.17
AT2G12280.1	Thale cress	plastid	1.3	5.56
AT2G12200.1	Thale cress	mitochondrion, plastid	0.52	3.17

Protein Annotations

KEGG:00670+6.3.4.3	KEGG:00720+6.3.4.3	EntrezGene:100777027	Gene3D:3.30.1510.10	Gene3D:3.40.50.300	MapMan:7.5.7.1
EMBL:ACUP02006443	InterPro:Formate_THF_ligase	InterPro:Formate_THF_ligase_CS	EnsemblPlantsGene:GLYMA_10G112700	GO:GO:0000166	GO:GO:0003674
GO:GO:0003824	GO:GO:0004329	GO:GO:0004477	GO:GO:0004488	GO:GO:0005488	GO:GO:0005524
GO:GO:0005575	GO:GO:0005622	GO:GO:0005623	GO:GO:0005737	GO:GO:0006139	GO:GO:0008150
GO:GO:0008152	GO:GO:0009058	GO:GO:0009113	GO:GO:0009257	GO:GO:0009987	GO:GO:0016787
GO:GO:0055114	UniProt:I1L9Z8	EnsemblPlants:KRH33286	ProteinID:KRH33286	ProteinID:KRH33286.1	InterPro:P-loop_NTPase
PFAM:PF01268	ScanProsite:PS00721	PANTHER:PTHR43274	MetaCyc:PWY-1722	MetaCyc:PWY-2161	MetaCyc:PWY-2201
MetaCyc:PWY-3841	SUPFAM:SSF52540	UniParc:UPI000294D52A	SEG:seg	:	:

Description

hypothetical protein

Coordinates

chr10:+:27236594..27239785

Molecular Weight (calculated)

42615.9 Da

IEP (calculated)

8.123

GRAVY (calculated)

0.033

Length

386 amino acids

Sequence
(BLAST)

001: MEVVIVAIAA TLGNLLMGWD SSTIADKMMS STTVRKLQVV SPVPVDIDIA NSVEPVHISQ IAKDLNLSPN HYDLYGKYKA KVLLSVLDEL QGSEDGYYVV
101: VGGITPTPLG EGKSTTTVGL CQALGAFLDK KVVTCLRQPS QGPTFGIKGG AAGGGYSQVI PMDEFNLHLT GDIHAITAAN NLLAAAIDTR IFHESTQSDK
201: ALFNRLCPPN KEGKRSFSDV MFRRLTKLGI SKTNPDDLTP EEVNKFARLD IDPNSITWRR VMDINDRFLR KIAIGQGLDE KGMVRETGFD ISVASEIVAV
301: LALTTSLADM RKRLGKMVIG NTRESHFTFC FMLDLITEMA QETTRALRTG YILVYIYIYG STNIFKKKTI LFCFNHLKPI FYFCLH

Best Arabidopsis Sequence Match ( AT1G50480.1 )
(BLAST)

001: MSSSTRKLEV VSPVPADIDI ANSVEPLHIS EIAKDLNINP LHYDLYGKYK AKVLLSAFDE LQGQEDGYYV VVGGITPTPL GEGKSTTTVG LCQALGAYLD
101: KKVVTCLRQP SQGPTFGIKG GAAGGGYSQV IPMDEFNLHL TGDIHAITAS NNLLAAAIDT RIFHETSQSD KALFNRLCPP NKEGKRSFSD IMFRRLTKLG
201: ISKTSPEELT PEEIKKFARL DIDPASITWR RVMDVNDRFL RKITIGQGPE EKGMTRETGF DISVASEIMA VLALTTSLGD MRERLGKMVI GNSKAGDPIT
301: ADDLGVGGAL TVLMKDAINP TLMQTLEGTP VLVHAGPFAN IAHGNSSIVA DKIALKLVGP GGFVVTEAGF GSDIGTEKFM NIKCRYSGLT PQCAIVVATV
401: RALKMHGGGP DVVAGRPLDR AYVSENVSLV EAGCVNLAKH ISNTKAYGVN VIVAVNMFAT DTEAELNAVR KFSMDAGAFD AVVCSHHAHS GKGAVDLGIA
501: VEKACQNITQ PLRFLYPLDI GIKDKIEAIA KSYGASGVEY SDQAEKQIEM YTQQGFSNLP ICMSKTQYSF SHDASKKGAP SGFVLPIRDV RGSIGAGFIY
601: PLVGTMSTMP GLPTRPCFYE IDIDTETGKV RGLS

Arabidopsis Description

THFSFormate--tetrahydrofolate ligase [Source:UniProtKB/Swiss-Prot;Acc:Q9SPK5]
SUBAcon: [cytosol]

Hydropathy Plot

About CropPAL

The Protein Annotated Locations Database (CropPAL) houses large scale proteomic and GFP localization data from published experimental studies in Soybean (Glycine max), Maize (Zea mays), Wheat (Triticum aestivum), Barley (Hordeum vulgare), Rice (Oryza sativa), Field mustard (Brassica rapa), Canola (Brassica napus), Sorghum (Sorghum bicolor), Potato (Solanum tuberosum), Tomato (Solanum lycopersicum), Banana (Musa acuminata) and Wine grape (Vitis vinifera) as well as precomputed predictions for protein subcellular localizations using protein sequences.