CropPAL

Subcellular Localization

min:

: max

Winner_takes_all: endoplasmic reticulum

Predictor Summary:

plastid 2
golgi 3
mitochondrion 4
cytosol 1

Predictors

GFP

MS/MS

Papers

nucleus: 21132161
endoplasmic reticulum: 27224218
endoplasmic reticulum: 28499913

msms PMID: 21132161 doi

B Cooper, KB Campbell, J Feng, WM Garrett, R Frederick

Soybean Genomics and Improvement Laboratory, USDA-ARS, Beltsville, MD 20705, USA. bret.cooper@ars.usda.gov

msms PMID: 28499913 doi

S Komatsu, X Wang, X Yin, Y Nanjo, H Ohyanagi, K Sakata

Department of Life Science and Informatics, Maebashi Institute of Technology, Maebashi 371-0816, Japan. Electronic address: ksakata@maebashi-it.ac.jp., Institute of Crop Science, National Agriculture and Food Research Organization, Tsukuba 305-8518, Japan., Institute of Crop Science, National Agriculture and Food Research Organization, Tsukuba 305-8518, Japan; Faculty of Life and Environmental Sciences, University of Tsukuba, Tsukuba 305-8572, Japan., Institute of Crop Science, National Agriculture and Food Research Organization, Tsukuba 305-8518, Japan; Faculty of Life and Environmental Sciences, University of Tsukuba, Tsukuba 305-8572, Japan. Electronic address: komatsu.setsuko.fu@u.tsukuba.ac.jp., King Abdullah University of Science and Technology (KAUST), Computational Bioscience Research Center (CBRC), Thuwal 23955-6900, Saudi Arabia.

msms PMID: 27224218 doi

X Wang, S Komatsu

Graduate School of Life and Environmental Sciences, University of Tsukuba , Tsukuba 305-8572, Japan., National Institute of Crop Science, National Agriculture and Food Research Organization , Tsukuba 305-8518, Japan.

PPI

Inferred distinct locusB in Crop

locusB	locations
KRG91803
KRH34983
KRH34984

Inferred from Arabidopsis experimental PPI

Ath locusA	locusB	Ath locusB	Paper
AT3G21160.1	KRG91803	AT4G09570.1	19130088
AT3G21160.1	KRH34983	AT4G09570.1	19130088
AT3G21160.1	KRH34984	AT4G09570.1	19130088

Homology

Paralog

locus	Identity	Homology Identity

Ortholog

locus	Homology Species	Location	Identity	Homology Identity
KRH44626	Soybean	mitochondrion	94.1	93.77
KRH58075	Soybean	extracellular	13.19	80.85
VIT_09s0054g00370.t01	Wine grape	golgi, mitochondrion	79.69	79.83
VIT_09s0054g00230.t01	Wine grape	cytosol	47.05	79.01
PGSC0003DMT400023755	Potato	cytosol	35.59	78.24
VIT_09s0054g00310.t01	Wine grape	cytosol	12.15	76.09
AT1G51590.1	Thale cress	golgi, mitochondrion	72.4	74.46
Bra018917.1-P	Field mustard	golgi, mitochondrion, plastid	71.88	73.8
CDY46554	Canola	golgi, mitochondrion	71.7	73.75
Solyc03g123900.2.1	Tomato	extracellular, nucleus	74.13	73.75
CDY55669	Canola	golgi, mitochondrion, plastid	71.7	73.62
CDY52948	Canola	golgi, mitochondrion	71.01	73.3
CDY22853	Canola	golgi, mitochondrion, plastid	71.35	73.26
Solyc06g063260.2.1	Tomato	golgi, mitochondrion	71.7	73.1
AT3G21160.1	Thale cress	golgi	70.49	70.98
CDX82213	Canola	golgi, mitochondrion	68.75	70.59
Bra001816.1-P	Field mustard	golgi, mitochondrion	69.62	70.23
CDY32409	Canola	golgi, mitochondrion	68.23	70.05
CDY50074	Canola	golgi, mitochondrion, plastid	69.62	69.62
Bra031256.1-P	Field mustard	golgi, mitochondrion, plastid	69.79	69.43
CDY19581	Canola	golgi, mitochondrion, plastid	69.27	68.91
KRH19110	Soybean	cytosol	42.71	65.78
KRH02771	Soybean	golgi, plastid	64.93	64.37
Bra014291.1-P	Field mustard	mitochondrion	72.57	63.53
KRH11192	Soybean	endoplasmic reticulum	36.46	33.18
KRH21064	Soybean	endoplasmic reticulum	36.28	32.97
KRH57025	Soybean	extracellular	11.11	31.53
KRH03356	Soybean	endoplasmic reticulum, golgi	24.13	24.3
KRH34374	Soybean	endoplasmic reticulum, golgi, vacuole	25.0	23.26

Protein Annotations

KEGG:00510+3.2.1.113	KEGG:00513+3.2.1.113	Gene3D:1.50.10.10	EntrezGene:100819189	MapMan:18.1.1.6.3	InterPro:6hp_glycosidase-like_sf
EMBL:ACUP02004144	ncoils:Coil	EnsemblPlantsGene:GLYMA_07G020200	GO:GO:0000139	GO:GO:0003674	GO:GO:0003824
GO:GO:0004571	GO:GO:0005488	GO:GO:0005509	GO:GO:0005575	GO:GO:0005622	GO:GO:0005623
GO:GO:0005737	GO:GO:0005783	GO:GO:0005794	GO:GO:0006491	GO:GO:0008150	GO:GO:0008152
GO:GO:0009987	GO:GO:0016020	GO:GO:0016021	GO:GO:0016787	GO:GO:0016798	GO:GO:0019538
InterPro:Glyco_hydro_47	UniProt:I1KGP3	InterPro:IPR012341	EnsemblPlants:KRH47289	ProteinID:KRH47289	ProteinID:KRH47289.1
PFAM:PF01532	PRINTS:PR00747	PANTHER:PTHR11742	PANTHER:PTHR11742:SF42	SUPFAM:SSF48225	InterPro:Seven-hairpin_glycosidases
TMHMM:TMhelix	UniParc:UPI00023380AB	SEG:seg	:	:	:

Description

hypothetical protein

Coordinates

chr7:-:1577933..1584676

Molecular Weight (calculated)

65157.1 Da

IEP (calculated)

6.802

GRAVY (calculated)

-0.417

Length

576 amino acids

Sequence
(BLAST)

001: MARGSRSVTS SSKWRYCNPS YYLKRPKRLA LLFIVFVGVS FIVWDRQTLV REHQVEISEL RKEVTGLQNL LDDLNNKQGG TSGKTDLRKA TKTSKEVLDD
101: PIDIERREKV KEAMLHAWGS YEKYAWGQDE LQPQSKNGVN SFGGLGATLI DSLDTLYIMG LNEQFQKARE WVANSLDFNK DYEASVFETT IRVVGGLLSA
201: YDLSGDKVFL NKAIEIADRL LPAWNTPTGI PYNIINLSHG RAHNPSWTGG ESILADSGTE QLEFIVLSQR TGDPKYQQKV ENVIAQLNKT FPDDGLLPIY
301: INPHSGAAGY SPITFGAMGD SFYEYLLKVW IQGNKTSAVK HYRDMWEKSM KGLSSLIRRS TPSSFAYICE KNGGSLTDKM DELACFAPGM IALGSFGYSA
401: DDDSQKFLSL AEELAWTCYN FYQSTPTKLA GENYFFHSGQ DMSVGTSWNI LRPETVESLF YLWRLTGNKT YQEWGWNIFQ AFEKNSRIES GYVGLKDVNS
501: GVKDNMMQSF FLAETLKYFY LLFSPSSVIP LDEWVFNTEA HPLRIVTRHE EGLVKNLNEK QKPFSRIGGR KEGRSG

Best Arabidopsis Sequence Match ( AT1G51590.1 )
(BLAST)

001: MARSRSISGY GIWKYLNPAY YLRRPRRLAL LFIVFVSVSM LVWDRINLAR EHEVEVFKLN EEVSRLEQML EELNGGVGNK PLKTLKDAPE DPVDKQRRQK
101: VKEAMIHAWS SYEKYAWGKD ELQPRTKDGT DSFGGLGATM VDSLDTLYIM GLDEQFQKAR EWVASSLDFD KDYDASMFET TIRVVGGLLS AYDLSGDKMF
201: LEKAKDIADR LLPAWNTPTG IPYNIINLRN GNAHNPSWAA GGDSILADSG TEQLEFIALS QRTGDPKYQQ KVEKVITELN KNFPADGLLP IYINPDNANP
301: SYSTTTFGAM GDSFYEYLLK VWVQGNKTSA VKPYRDMWEK SMKGLLSLVK KSTPSSFTYI CEKNGNNLID KMDELACFAP GMLALGASGY GPDEEKKFLS
401: LAGELAWTCY NFYQSTPTKL AGENYFFTAG QDMSVGTSWN ILRPETVESL FYLWRLTGNK TYQEWGWNIF QAFEKNSRVE SGYVGLKDVN TGAKDNKMQS
501: FFLAETLKYL YLLFSPSSVI SLDEWVFNTE AHPLKIVARN DPRKPTIALR QRKFGHQINV

Arabidopsis Description

MNS1Mannosyl-oligosaccharide 1,2-alpha-mannosidase MNS1 [Source:UniProtKB/Swiss-Prot;Acc:Q9C512]
SUBAcon: [mitochondrion,golgi]

Hydropathy Plot

About CropPAL

The Protein Annotated Locations Database (CropPAL) houses large scale proteomic and GFP localization data from published experimental studies in Soybean (Glycine max), Maize (Zea mays), Wheat (Triticum aestivum), Barley (Hordeum vulgare), Rice (Oryza sativa), Field mustard (Brassica rapa), Canola (Brassica napus), Sorghum (Sorghum bicolor), Potato (Solanum tuberosum), Tomato (Solanum lycopersicum), Banana (Musa acuminata) and Wine grape (Vitis vinifera) as well as precomputed predictions for protein subcellular localizations using protein sequences.