CropPAL

Subcellular Localization

min:

: max

Winner_takes_all: endoplasmic reticulum

Predictor Summary:

extracellular 7
endoplasmic reticulum 7
vacuole 5
plasma membrane 6
golgi 6
mitochondrion 1
plastid 1
nucleus 1

Predictors

GFP

MS/MS

Papers

Winner Takes All:	endoplasmic reticulum
Any Predictor:	mitochondrion, nucleus, plastid, secretory
BaCelLo:	secretory
EpiLoc:	endoplasmic reticulum
iPSORT:	secretory
MultiLoc:	golgi
Plant-mPloc:	extracellular, mitochondrion, nucleus, plasma membrane, plastid
Predotar:	secretory
PProwler:	secretory
TargetP:	secretory
WoLF PSORT:	extracellular
YLoc:	endoplasmic reticulum

nucleus: 21132161
endoplasmic reticulum: 27224218
endoplasmic reticulum: 28499913
mitochondrion: 28499913

msms PMID: 21132161 doi

B Cooper, KB Campbell, J Feng, WM Garrett, R Frederick

Soybean Genomics and Improvement Laboratory, USDA-ARS, Beltsville, MD 20705, USA. bret.cooper@ars.usda.gov

msms PMID: 28499913 doi

S Komatsu, X Wang, X Yin, Y Nanjo, H Ohyanagi, K Sakata

Department of Life Science and Informatics, Maebashi Institute of Technology, Maebashi 371-0816, Japan. Electronic address: ksakata@maebashi-it.ac.jp., Institute of Crop Science, National Agriculture and Food Research Organization, Tsukuba 305-8518, Japan., Institute of Crop Science, National Agriculture and Food Research Organization, Tsukuba 305-8518, Japan; Faculty of Life and Environmental Sciences, University of Tsukuba, Tsukuba 305-8572, Japan., Institute of Crop Science, National Agriculture and Food Research Organization, Tsukuba 305-8518, Japan; Faculty of Life and Environmental Sciences, University of Tsukuba, Tsukuba 305-8572, Japan. Electronic address: komatsu.setsuko.fu@u.tsukuba.ac.jp., King Abdullah University of Science and Technology (KAUST), Computational Bioscience Research Center (CBRC), Thuwal 23955-6900, Saudi Arabia.

msms PMID: 27224218 doi

X Wang, S Komatsu

Graduate School of Life and Environmental Sciences, University of Tsukuba , Tsukuba 305-8572, Japan., National Institute of Crop Science, National Agriculture and Food Research Organization , Tsukuba 305-8518, Japan.

PPI

Inferred distinct locusB in Crop

locusB	locations
KRH52351
KRH55068
KRH61684

Inferred from Arabidopsis experimental PPI

Ath locusA	locusB	Ath locusB	Paper
AT2G25110.1	KRH52351	AT2G25110.1	19763086
AT2G25110.1	KRH61684	AT2G25110.1	19763086
AT2G25110.1	KRH55068	AT3G62600.1	19763086

Homology

Paralog

locus	Identity	Homology Identity

Ortholog

locus	Homology Species	Location	Identity	Homology Identity
KRH52351	Soybean	endoplasmic reticulum	95.95	97.71
VIT_04s0008g05820.t01	Wine grape	endoplasmic reticulum, golgi, vacuole	82.88	85.19
PGSC0003DMT400036615	Potato	endoplasmic reticulum, extracellular, golgi	77.93	81.6
AT2G25110.1	Thale cress	endoplasmic reticulum, extracellular, golgi	78.38	79.82
Bra032038.1-P	Field mustard	endoplasmic reticulum, extracellular, golgi	78.38	79.82
CDY35374	Canola	endoplasmic reticulum, extracellular, golgi	78.38	79.82
CDX76770	Canola	endoplasmic reticulum, extracellular, plasma membrane	78.83	79.55
CDY56283	Canola	endoplasmic reticulum, extracellular, plasma membrane	78.83	79.19
Bra007818.1-P	Field mustard	endoplasmic reticulum, plasma membrane	77.93	79.0
CDY55710	Canola	endoplasmic reticulum, extracellular, golgi	77.48	78.9
TraesCS7D01G327100.1	Wheat	endoplasmic reticulum	74.77	76.5
TraesCS7A01G330300.1	Wheat	endoplasmic reticulum	74.32	76.04
Os08t0278900-01	Rice	endoplasmic reticulum	73.87	75.58
EES13770	Sorghum	endoplasmic reticulum, golgi, vacuole	73.87	75.58
GSMUA_Achr3P13410_001	Banana	endoplasmic reticulum, golgi, vacuole	73.42	75.46
GSMUA_Achr6P04010_001	Banana	endoplasmic reticulum, golgi, plasma membrane	71.17	73.83
Os08t0440500-01	Rice	endoplasmic reticulum, golgi, vacuole	70.27	71.89
EES15057	Sorghum	endoplasmic reticulum	68.02	69.91
TraesCS7D01G160300.1	Wheat	endoplasmic reticulum, plasma membrane, vacuole	67.57	69.44
TraesCS7B01G063900.1	Wheat	golgi	67.12	68.98
Zm00001d050430_P003	Maize	extracellular	66.67	68.52
Zm00001d032157_P001	Maize	endoplasmic reticulum, extracellular, golgi	66.67	68.52
TraesCS7A01G159300.1	Wheat	endoplasmic reticulum, plasma membrane, vacuole	67.12	67.42
TraesCS7B01G231000.1	Wheat	endoplasmic reticulum	54.95	64.21
GSMUA_Achr6P04030_001	Banana	cytosol	27.93	52.99
GSMUA_Achr3P00940_001	Banana	cytosol	17.57	49.37
Solyc03g117020.2.1	Tomato	nucleus	76.13	45.8
HORVU7Hr1G079030.2	Barley	plastid	49.55	42.64

Protein Annotations

EntrezGene:100811638	MapMan:19.1.1.2.1.3	Gene3D:2.80.10.50	EMBL:ACUP02002181	EMBL:BT099289	UniProt:C6TNG6
EnsemblPlantsGene:GLYMA_04G061900	GO:GO:0000030	GO:GO:0003674	GO:GO:0003824	GO:GO:0005575	GO:GO:0005975
GO:GO:0006464	GO:GO:0006486	GO:GO:0008150	GO:GO:0008152	GO:GO:0009058	GO:GO:0009987
GO:GO:0016020	GO:GO:0016740	GO:GO:0019538	GO:GO:0097502	InterPro:GlyclTrfase_39-like	InterPro:IPR016093
EnsemblPlants:KRH61684	ProteinID:KRH61684	ProteinID:KRH61684.1	InterPro:MIR_dom_sf	InterPro:MIR_motif	PFAM:PF02815
PFscan:PS50919	PANTHER:PTHR10050	PANTHER:PTHR10050:SF46	SMART:SM00472	SUPFAM:SSF82109	SignalP:SignalP-noTM
UniParc:UPI0001B17036	SEG:seg	:	:	:	:

Description

hypothetical protein

Coordinates

chr4:+:5091277..5095032

Molecular Weight (calculated)

24336.6 Da

IEP (calculated)

7.366

GRAVY (calculated)

-0.410

Length

222 amino acids

Sequence
(BLAST)

001: MALGFFALAI FLFLTLDSDI APSSTASASA SAASSEGVEV QVTYGTVLKL MHEKTKFRLH SHDVPYGSGS GQQSVTGFPN VDDSNSYWIV RPEPGTSAKQ
101: GDPIKSGTII RLQHMRTRKW LHSHLHASPI SGNLEVSCFG GESESDTGDY WKLIIEGSGK TWKQDQRIRL QHIDTGGYLH SHDKKYSRIA GGQQEVCGVR
201: EKRADNVWLA AEGVYLPVTE SK

Best Arabidopsis Sequence Match ( AT2G25110.1 )
(BLAST)

001: MALGFFCLAI FLYLSLDPDS GYTSASAAAS GKEGVEITYG SAIKLMHEKT KFRLHSHDVP YGSGSGQQSV TGFPGVVDSN SYWIVKPVPG TTEKQGDAVK
101: SGATIRLQHM KTRKWLHSHL HASPISGNLE VSCFGDDTNS DTGDHWKLII EGSGKTWKQD QRVRLQHIDT SGYLHSHDKK YQRIAGGQQE VCGIREKKAD
201: NIWLAAEGVY LPLNESSK

Arabidopsis Description

SDF2Stromal cell-derived factor 2-like protein [Source:UniProtKB/Swiss-Prot;Acc:Q93ZE8]
SUBAcon: [endoplasmic reticulum,golgi,extracellular]

Hydropathy Plot

About CropPAL

The Protein Annotated Locations Database (CropPAL) houses large scale proteomic and GFP localization data from published experimental studies in Soybean (Glycine max), Maize (Zea mays), Wheat (Triticum aestivum), Barley (Hordeum vulgare), Rice (Oryza sativa), Field mustard (Brassica rapa), Canola (Brassica napus), Sorghum (Sorghum bicolor), Potato (Solanum tuberosum), Tomato (Solanum lycopersicum), Banana (Musa acuminata) and Wine grape (Vitis vinifera) as well as precomputed predictions for protein subcellular localizations using protein sequences.