CropPAL

Subcellular Localization

min:

: max

Winner_takes_all: nucleus

Predictor Summary:

plastid 4
nucleus 1
cytosol 2
mitochondrion 2
extracellular 1
endoplasmic reticulum 1
vacuole 1
plasma membrane 1
golgi 1

Predictors

GFP

MS/MS

Papers

Winner Takes All:	nucleus
Any Predictor:	cytosol, mitochondrion, nucleus, plastid, secretory
BaCelLo:	plastid
iPSORT:	plastid
MultiLoc:	nucleus
Plant-mPloc:	cytosol, plastid
Predotar:	secretory
PProwler:	mitochondrion
TargetP:	mitochondrion
WoLF PSORT:	plastid
YLoc:	cytosol

nucleus: 21132161
nucleus: 28499913

msms PMID: 21132161 doi

B Cooper, KB Campbell, J Feng, WM Garrett, R Frederick

Soybean Genomics and Improvement Laboratory, USDA-ARS, Beltsville, MD 20705, USA. bret.cooper@ars.usda.gov

msms PMID: 28499913 doi

S Komatsu, X Wang, X Yin, Y Nanjo, H Ohyanagi, K Sakata

Department of Life Science and Informatics, Maebashi Institute of Technology, Maebashi 371-0816, Japan. Electronic address: ksakata@maebashi-it.ac.jp., Institute of Crop Science, National Agriculture and Food Research Organization, Tsukuba 305-8518, Japan., Institute of Crop Science, National Agriculture and Food Research Organization, Tsukuba 305-8518, Japan; Faculty of Life and Environmental Sciences, University of Tsukuba, Tsukuba 305-8572, Japan., Institute of Crop Science, National Agriculture and Food Research Organization, Tsukuba 305-8518, Japan; Faculty of Life and Environmental Sciences, University of Tsukuba, Tsukuba 305-8572, Japan. Electronic address: komatsu.setsuko.fu@u.tsukuba.ac.jp., King Abdullah University of Science and Technology (KAUST), Computational Bioscience Research Center (CBRC), Thuwal 23955-6900, Saudi Arabia.

PPI

Inferred distinct locusB in Crop

locusB	locations
KRH30074
KRH62827

Inferred from Arabidopsis experimental PPI

Ath locusA	locusB	Ath locusB	Paper
AT1G69200.1	KRH30074	AT3G06730.1	20511297
AT1G69200.1	KRH62827	AT3G06730.1	20511297

Homology

Paralog

locus	Identity	Homology Identity

Ortholog

locus	Homology Species	Location	Identity	Homology Identity
KRH70953	Soybean	nucleus	93.54	93.54
VIT_01s0011g00240.t01	Wine grape	mitochondrion, plastid	65.44	66.37
CDY54150	Canola	endoplasmic reticulum, extracellular, golgi, plasma membrane, vacuole	48.52	58.9
CDX95956	Canola	plastid	60.91	57.4
AT1G69200.1	Thale cress	plastid	61.43	57.33
CDY40900	Canola	plastid	61.43	57.14
Bra004365.1-P	Field mustard	plastid	61.26	56.89
Solyc05g012100.2.1	Tomato	nucleus	59.34	52.71
PGSC0003DMT400072750	Potato	mitochondrion	59.69	52.62
EER93838	Sorghum	mitochondrion	50.26	49.4
Os03t0602600-01	Rice	nucleus	50.26	48.9
HORVU4Hr1G032860.1	Barley	cytosol	27.75	48.33
TraesCS2D01G014800.1	Wheat	plastid	48.69	48.27
HORVU7Hr1G106650.1	Barley	cytosol	17.8	48.11
HORVU2Hr1G002260.1	Barley	plastid	48.34	47.92
HORVU2Hr1G002180.1	Barley	plastid	48.34	47.92
TraesCS2A01G111500.1	Wheat	plastid	47.99	47.58
TraesCS2B01G004000.1	Wheat	plastid	47.64	47.23
Zm00001d033181_P001	Maize	plastid	49.74	43.12
KRH65986	Soybean	nucleus	30.19	39.32
KRH75791	Soybean	nucleus	31.94	39.19
KRH10125	Soybean	endoplasmic reticulum, nucleus	20.24	35.15
KRH23212	Soybean	endoplasmic reticulum	20.07	34.74
KRH29617	Soybean	endoplasmic reticulum	19.9	34.44
KRH24618	Soybean	cytosol, peroxisome, plastid	19.72	32.56
KRH34333	Soybean	cytosol, nucleus	18.5	32.42
KRG92467	Soybean	cytosol, nucleus	18.32	32.11
KRH35400	Soybean	cytosol, nucleus	18.85	31.67
KRG91421	Soybean	cytosol	18.32	30.79
KRH09171	Soybean	cytosol	18.67	30.66
KRH17371	Soybean	cytosol	13.96	30.53
KRH73007	Soybean	plastid	19.9	29.77
KRH38640	Soybean	cytosol, golgi, plastid	18.15	29.13
KRH40559	Soybean	plastid	17.28	27.42
KRH00623	Soybean	plastid	14.83	26.65

Protein Annotations

EMBL:ACUP02000117	EnsemblPlants:KRH75089	EnsemblPlantsGene:GLYMA_01G062100	EntrezGene:100786248	Gene3D:3.40.1190.20	InterPro:IPR029056
InterPro:PfkB_dom	InterPro:Ribokinase-like	ncoils:Coil	PANTHER:PTHR43085	PANTHER:PTHR43085:SF2	PFAM:PF00294
ProteinID:KRH75089	ProteinID:KRH75089.1	SEG:seg	SUPFAM:SSF53613	UniParc:UPI000294A0C6	UniProt:I1J631
MapMan:15.6.1.2.2.12	:	:	:	:	:

Description

hypothetical protein

Coordinates

chr1:-:8627176..8631264

Molecular Weight (calculated)

63959.8 Da

IEP (calculated)

5.833

GRAVY (calculated)

-0.490

Length

573 amino acids

Sequence
(BLAST)

001: MAYLSFPQFL SLPRYHMTWS YCCTSFNTVQ LGKLGPGCKW GHVAMARKKA SLDSSIEEEP SDNESVVEKK TTRSSKIKKT TVRTRKKTKD ESPGDTDDAS
101: IEGSSAAASD DSKKTRRTRK KGASSSAGLE EKKEVKEEKK VRRRKKTEEE KLIVEDKGSE AEISDQDEPS FLENVEDDSD SGLELIKDDG EDISFTYGWP
201: PLVCCFGAVQ HAFVPSGRPA NRLINHEIHE SMKDALWSPE KFVRAPGGSA GSVAIALATL GGKVAFMGKL ADDDYGQAML YYMNANNVQT RSVRIDSKRA
301: TAVSLMKVGK RSRLKMSCVK PCAEDSLTKS ELNFDVLKEA KMFYFNTHSL LDRNMRSTTL QAIKISKHFG GVIFYDLNLP IPLWHSSEET MMFIQQAWNL
401: ADIIEVTKQE LEFLCGITPS EEFDTKNNAR SKFVHYEPEV VSPLWHENLK VLFVTNGTSK IHYYTKELDG AVLGMEDAPI TPFTCDMSAT GDGIVAALMR
501: MLTVQPDLLT DKGYLEHSIK YAIDCGVIDQ WILGRVRGFP PHEDMEEDIT PDSNGIRSIS ETEYRTAVGS ASE

Best Arabidopsis Sequence Match ( AT1G69200.1 )
(BLAST)

001: MASLSFTQFL SFPRCNADVP CLLQSHGFVK FRGERWNGKQ SFSMAAGRRK LSESAPLEEE GNDGNGAVVG KKPSKSVKRT TKKKVVVKDE PLEEISEFLV
101: DNDDVLDKES IVSALKPKKT RTRKKAAAAS SDVEEVKTEK KVRRKRTVKK DKDVEDDLAT IMDAEVSDVE EALAVESTDT ESEEEEIDLS KHEGEDISHT
201: YGWPPLVCCF GSAQHAFVPS GRPANRLLDY ELHERMRDAK WAPEKYIRAP GGCAGGVAIA LASLGGKVAF MGKLGADDYG QAMLYYLNVC KVQTRSVKID
301: GKRVTACSTM KISKRGRLKS TCIKPCAEDS LSKSEINVDV LKEAKMFYFS THSLLDKKMM STTIQAIKIS KQLGNVIFYD LNLPLPLWHS SEETKSFIQE
401: VWNLADVIEI TKQELEFLCG IEPTEEFDTE NNDISKFVHY PPETVEQLWH ENLKVLFVTN GTSKIHYYTK EHNGAVSGME DVPITPFTRD MSASGDGIVA
501: GLIRMLTVQP DLMNNKGYLE RTARYAIECG IIDQWLLAQT RGYPPKDDME EEEDDEEEDE VESDPNGIRS ITEKEYRTSK PYDEPDGPYV MKPVEEREYK
601: KLELVGSMFE DGSL

Arabidopsis Description

FLN2Fructokinase-like 2, chloroplastic [Source:UniProtKB/Swiss-Prot;Acc:F4I0K2]
SUBAcon: [plastid]

Hydropathy Plot

About CropPAL

The Protein Annotated Locations Database (CropPAL) houses large scale proteomic and GFP localization data from published experimental studies in Soybean (Glycine max), Maize (Zea mays), Wheat (Triticum aestivum), Barley (Hordeum vulgare), Rice (Oryza sativa), Field mustard (Brassica rapa), Canola (Brassica napus), Sorghum (Sorghum bicolor), Potato (Solanum tuberosum), Tomato (Solanum lycopersicum), Banana (Musa acuminata) and Wine grape (Vitis vinifera) as well as precomputed predictions for protein subcellular localizations using protein sequences.