Factsheet for Os01t0743500-01

Subcellular Localization

min:

: max

Winner_takes_all: plasma membrane, plastid

Predictor Summary:

cytosol 3
plastid 2
mitochondrion 1
peroxisome 1

Predictors

GFP

MS/MS

Papers

plasma membrane: 27800704
plastid: 27992503

msms PMID: 27800704 doi

J Cao, C Yang, L Li, L Jiang, Y Wu, C Wu, Q Bu, G Xia, X Liu, Y Luo, J Liu

Huai'an Institute of Agricultural Sciences, Huai'an, 223001, China., Institute of Analytical Chemistry and Synthetic and Functional Biomolecules Center, College of Chemistry and Molecular Engineering, Peking University, Beijing, 100871, China; and., Northeast Institute of Geography and Agroecology, Key Laboratory of Soybean Molecular Design Breeding, Chinese Academy of Sciences, Harbin, 150081, China., State Key Laboratory of Plant Genomics, Institute of Microbiology, Chinese Academy of Sciences, Beijing, 100101, China.

msms PMID: 27992503 doi

S Xing, X Meng, L Zhou, H Mujahid, C Zhao, Y Zhang, C Wang, Z Peng

Department of Biochemistry, Molecular Biology, Entomology and Plant Pathology, Mississippi State University, Starkville, Mississippi, United States of America., Institute of Food Crops, Jiangsu Academy of Agricultural Sciences, Jiangsu High Quality Rice Research and Development Center, Nanjing Branch of China National Center for Rice Improvement, Nanjing, Jiangsu, China.

PPI

No PPI Data

Homology

Paralog

locus	Identity	Homology Identity

Ortholog

locus	Homology Species	Location	Identity	Homology Identity
Zm00001d012764_P001	Maize	cytosol	76.75	90.34
EES01460	Sorghum	cytosol	90.77	89.54
TraesCS3A01G275600.2	Wheat	cytosol, peroxisome, plastid	84.79	87.32
TraesCS3B01G309300.1	Wheat	mitochondrion	90.26	85.02
HORVU3Hr1G070850.1	Barley	mitochondrion	88.72	83.31
GSMUA_AchrUn_...	Banana	cytosol	79.66	78.98
VIT_11s0016g03210.t01	Wine grape	cytosol	78.63	77.83
Os05t0186300-01	Rice	cytosol, plasma membrane	75.56	77.54
AT2G19900.1	Thale cress	cytosol	76.92	77.45
TraesCS3D01G275500.1	Wheat	cytosol	90.6	76.81
KRH74263	Soybean	cytosol	76.92	76.14
Bra039029.1-P	Field mustard	cytosol	75.73	76.12
KRH07269	Soybean	cytosol	76.75	75.97
CDY35901	Canola	cytosol	75.04	75.43
KRH60218	Soybean	mitochondrion	77.61	71.72
CDX82748	Canola	cytosol	69.91	70.15
KRH41507	Soybean	cytosol, peroxisome, plastid	66.15	68.98
Os01t0188400-01	Rice	plastid	74.53	68.23
Os01t0723400-04	Rice	plastid	74.02	67.34
KRH12915	Soybean	cytosol	12.48	65.77
Os02t0665000-01	Rice	cytosol	20.17	63.44
Os07t0496200-01	Rice	mitochondrion	40.68	38.26
Os10t0503500-01	Rice	mitochondrion	40.34	38.06

Protein Annotations

Gene3D:3.40.50.10380	Gene3D:3.40.50.720	EntrezGene:4326552	MapMan:5.1.1.4	EMBL:AK073858	ProteinID:BAD87910.1
ProteinID:BAF06133.1	ProteinID:BAS74297.1	GO:GO:0000166	GO:GO:0003674	GO:GO:0003824	GO:GO:0004470
GO:GO:0004471	GO:GO:0004473	GO:GO:0005488	GO:GO:0005575	GO:GO:0005622	GO:GO:0005623
GO:GO:0005737	GO:GO:0006090	GO:GO:0006108	GO:GO:0008150	GO:GO:0008152	GO:GO:0009507
GO:GO:0009536	GO:GO:0009987	GO:GO:0016491	GO:GO:0046872	GO:GO:0051287	GO:GO:0055114
InterPro:IPR037062	InterPro:Malic_NAD-bd	InterPro:Malic_N_dom	InterPro:Malic_N_dom_sf	InterPro:Malic_OxRdtase	InterPro:Malic_enzyme_CS
InterPro:NAD(P)-bd_dom_sf	EnsemblPlantsGene:Os01g0743500	EnsemblPlants:Os01t0743500-01	PFAM:PF00390	PFAM:PF03949	PIRSF:PIRSF000106
PRINTS:PR00072	ScanProsite:PS00331	PANTHER:PTHR23406	PANTHER:PTHR23406:SF35	UniProt:Q5JKW5	SMART:SM00919
SMART:SM01274	SUPFAM:SSF51735	SUPFAM:SSF53223	UniParc:UPI000021CE2B	RefSeq:XP_015614053.1	SEG:seg

Description

cytosolic NADP malic enzyme 3, endosperm-specific gene 13Cytosolic NADP malic enzyme. (Os01t0743500-01)

Coordinates

chr1:+:31076571..31081251

Molecular Weight (calculated)

64273.4 Da

IEP (calculated)

6.936

GRAVY (calculated)

-0.127

Length

585 amino acids

Sequence
(BLAST)

001: MAGRGEGNGA ATMAGVATGG VEDAYGEDRA TEDQPITPWA VCVASGHSLL RDPRHNKGLS FTEKERDAHY LRGLLPPVVL SQELQEKRLL QNVRQFQVPL
101: QRYMALMDLQ ERNERLFYKL LIDNVEELLP VVYTPTVGEA CQKYGSIFRR PQGLYISLKE KGRILELLRN WPEKSIQVIV VTDGERILGL GDLGCQGMGI
201: PVGKLALYTA LGGVRPSACL PITIDVGTNN EDLLKDEFYI GLRQKRATGQ EYSDLLDEFM AAIKQNYGQK VLVQFEDFAN YNAFTLLEKY RANNLVFNDD
301: IQGTAAVVLA GLIAAQKFVS GTLADHTFLF FGAGEAGTGI AELVALEISN QSKVPVEDAR KKIWLLDSKG LIVSSRKDSL QPFKKRYAHE HEPVKDLLDA
401: VKVIKPTALI GSAGVGQSFT KEVIEAMSSI NERPIILALS NPTSQSECTA EQAYSWSKGR AIFGSGSPFD PVKYNDKLFV PAQANNAYIF PGFGLGVVIS
501: GAIRVKDEMI LAAAEGLADQ VTPEHVDKGL IYPPFSCIRK ISANIAARVA AKAYDLGLAS HLPRPKDLVK YAESCMYSPI YRSYR

Best Arabidopsis Sequence Match ( AT2G19900.1 )
(BLAST)

001: MEKVTNSDLK SSVDGGVVDV YGEDSATIEH NITPWSLSVS SGYSLLRDPR YNKGLAFTEK ERDTHYLRGL LPPVVLDQKL QEKRLLNNIR QYQFPLQKYM
101: ALTELQERNE RLFYKLLIDN VEELLPIVYT PTVGEACQKF GSIFRRPQGL FISLKDKGKI LDVLKNWPER NIQVIVVTDG ERILGLGDLG CQGMGIPVGK
201: LALYSALGGV RPSACLPVTI DVGTNNEKLL NDEFYIGLRQ KRATGQEYSE LLNEFMSAVK QNYGEKVLIQ FEDFANHNAF ELLAKYSDTH LVFNDDIQGT
301: ASVVLAGLVS AQKLTNSPLA EHTFLFLGAG EAGTGIAELI ALYMSKQMNA SVEESRKKIW LVDSKGLIVN SRKDSLQDFK KPWAHEHEPV KDLLGAIKAI
401: KPTVLIGSSG VGRSFTKEVI EAMSSINERP LIMALSNPTT QSECTAEEAY TWSKGRAIFA SGSPFDPVEY EGKVFVSTQA NNAYIFPGFG LGLVISGAIR
501: VHDDMLLAAA EALAGQVSKE NYEKGMIYPS FSSIRKISAQ IAANVATKAY ELGLAGRLPR PKDIVKCAES SMYSPTYRLY R

Arabidopsis Description

NADP-ME1NADP-dependent malic enzyme 1 [Source:UniProtKB/Swiss-Prot;Acc:O82191]
SUBAcon: [cytosol]

Hydropathy Plot

About CropPAL

The Protein Annotated Locations Database (CropPAL) houses large scale proteomic and GFP localization data from published experimental studies in Soybean (Glycine max), Maize (Zea mays), Wheat (Triticum aestivum), Barley (Hordeum vulgare), Rice (Oryza sativa), Field mustard (Brassica rapa), Canola (Brassica napus), Sorghum (Sorghum bicolor), Potato (Solanum tuberosum), Tomato (Solanum lycopersicum), Banana (Musa acuminata) and Wine grape (Vitis vinifera) as well as precomputed predictions for protein subcellular localizations using protein sequences.