Factsheet for Os10t0503500-01

Subcellular Localization

min:

: max

Winner_takes_all: mitochondrion

Predictor Summary:

plastid 1
mitochondrion 9

Predictors

GFP

MS/MS

Papers

mitochondrion: 19010998
mitochondrion: 23027867

msms PMID: 23027867 doi

G Liu, H Tian, YQ Huang, J Hu, YX Ji, SQ Li, YQ Feng, L Guo, YG Zhu

State Key Laboratory of Hybrid Rice, College of Life Sciences, Wuhan University, Wuhan 430072, China.

msms PMID: 19010998 doi

S Huang, NL Taylor, R Narsai, H Eubel, J Whelan, AH Millar

Australian Research Council Centre of Excellence in Plant Energy Biology, M316, University of Western Australia, Crawley, 6009 Western Australia, Australia.

PPI

Inferred distinct locusB in Crop

locusB	locations
Os08t0242900-01

Inferred from Arabidopsis experimental PPI

Ath locusA	locusB	Ath locusB	Paper
AT4G00570.1	Os08t0242900-01	AT2G18876.1	21798944

Homology

Paralog

locus	Identity	Homology Identity

Ortholog

locus	Homology Species	Location	Identity	Homology Identity
KXG38219	Sorghum	mitochondrion	91.13	91.42
TraesCS1D01G164800.4	Wheat	mitochondrion, unclear	90.48	90.92
TraesCS1B01G190400.5	Wheat	mitochondrion	87.74	88.6
Zm00001d013911_P001	Maize	mitochondrion	86.94	84.22
GSMUA_Achr1P00210_001	Banana	mitochondrion	76.61	78.64
VIT_15s0046g03670.t01	Wine grape	mitochondrion	75.48	77.36
HORVU1Hr1G045720.1	Barley	plastid	90.65	77.3
Solyc01g094200.2.1	Tomato	plastid	74.35	76.71
Bra037347.1-P	Field mustard	mitochondrion	72.26	76.32
CDY51151	Canola	mitochondrion	74.35	75.95
CDY68746	Canola	mitochondrion	74.03	75.87
KRH00689	Soybean	mitochondrion	73.39	75.83
KRH48207	Soybean	mitochondrion	73.71	75.66
CDX74340	Canola	mitochondrion	74.03	75.49
CDY07209	Canola	mitochondrion	74.03	75.37
KRH65116	Soybean	mitochondrion	73.39	75.33
AT4G00570.1	Thale cress	mitochondrion	73.23	74.79
KRH40505	Soybean	mitochondrion	72.42	74.71
Bra000967.1-P	Field mustard	mitochondrion	74.03	74.03
PGSC0003DMT400000066	Potato	mitochondrion	74.52	73.68
Os07t0496200-01	Rice	mitochondrion	64.35	64.15
Os05t0186300-01	Rice	cytosol, plasma membrane	37.9	41.23
Os01t0743500-01	Rice	plasma membrane, plastid	38.06	40.34
Os02t0665000-01	Rice	cytosol	11.61	38.71
Os01t0188400-01	Rice	plastid	38.39	37.25
Os01t0723400-04	Rice	plastid	36.77	35.46

Protein Annotations

MapMan:2.3.9	Gene3D:3.40.50.10380	Gene3D:3.40.50.720	EntrezGene:4349051	ProteinID:ABB47851.2	ProteinID:BAF26912.1
ProteinID:BAT11549.1	GO:GO:0000166	GO:GO:0003674	GO:GO:0003824	GO:GO:0004470	GO:GO:0004471
GO:GO:0005488	GO:GO:0005575	GO:GO:0005622	GO:GO:0005623	GO:GO:0005737	GO:GO:0005739
GO:GO:0006090	GO:GO:0006108	GO:GO:0006139	GO:GO:0006355	GO:GO:0008150	GO:GO:0008152
GO:GO:0008270	GO:GO:0009058	GO:GO:0009507	GO:GO:0009536	GO:GO:0009987	GO:GO:0016491
GO:GO:0046872	GO:GO:0051287	GO:GO:0055114	InterPro:IPR037062	InterPro:Malic_NAD-bd	InterPro:Malic_N_dom
InterPro:Malic_N_dom_sf	InterPro:Malic_OxRdtase	InterPro:Malic_enzyme_CS	InterPro:NAD(P)-bd_dom_sf	EnsemblPlantsGene:Os10g0503500	EnsemblPlants:Os10t0503500-01
PFAM:PF00390	PFAM:PF03949	PIRSF:PIRSF000106	PRINTS:PR00072	ScanProsite:PS00331	PANTHER:PTHR23406
PANTHER:PTHR23406:SF32	UniProt:Q337E9	SMART:SM00919	SMART:SM01274	SUPFAM:SSF51735	SUPFAM:SSF53223
UniParc:UPI00005E1F47	RefSeq:XP_015614456.1	SEG:seg	:	:	:

Description

Similar to NAD-dependent malic enzyme 59 kDa isoform, mitochondrial precursor (EC 1.1.1.39) (NAD-ME). (Os10t0503500-01);Similar to Malic enzyme. (Os10t0503500-02);Similar to Malic enzyme. (Os10t0503500-03)

Coordinates

chr10:+:19218597..19224021

Molecular Weight (calculated)

68283.3 Da

IEP (calculated)

8.514

GRAVY (calculated)

-0.166

Length

620 amino acids

Sequence
(BLAST)

001: MWRHAARRSS AQIRRSGVMS SSSSPAAPAA AAGAAVPGPC IVHKRGTDIL HDPWFNKDTA FPMTERDRLG LRGLLPPRVM SFEQQYDRFI NSFRSLEHNT
101: RGEPDTIVAL AKWRILNRLH DRNETLYYRV LIDNIKDFAP IIYTPTVGLV CENYSGLFRR PRGMYFSAKD KGEMMSMIYN WPAEKVDMIV VTDGSRILGL
201: GDLGVQGIGI PIGKLDVYVA AAGINPQKVL PIMLDVGTNN TKLLEDKLYL GLRQPRLEGE EYLSVVDEFM EAVHARWPKA VVQFEDFQMK WAFETLQRYR
301: NRFCMFNDDV QGTAGVALAG LLGAVRAQGR PLSDFTKQKI VVVGAGSAGI GVLNMAKQAM LRMPGINRSG EGHNQFWVLD KDGLITKSRK GLDPAVARYA
401: RGFGPEEVQD LHEGASLVEV VRKVKPHVLL GLSGVGGIFN EEVLKAMKES DSPRPAIFAM SNPTTKAECT PEDVFKYVGD NAVFASGSPF SNVTLGNGRQ
501: GYANQANNMY LFPGIGLGAL LSGARHITDG MLQSAAECLA SYITDDEIRK GILFPSISSI RHITARVGAA VVRAAVDEDL AEGRCDVDAR DLKSMTEADT
601: VDFVARKMWY PIYSPLVNDK

Best Arabidopsis Sequence Match ( AT4G00570.1 )
(BLAST)

001: MMWKNIAGLS KAAAAARTHG SRRCFSTAIP GPCIVHKRGA DILHDPWFNK DTGFPLTERD RLGIRGLLPP RVMTCVQQCD RFIESFRSLE NNTKGEPENV
101: VALAKWRMLN RLHDRNETLY YRVLIDNIKD FAPIIYTPTV GLVCQNYSGL YRRPRGMYFS AKDKGEMMSM IYNWPAPQVD MIVITDGSRI LGLGDLGVQG
201: IGIPIGKLDM YVAAAGINPQ RVLPIMLDVG TNNEKLLQND LYLGVRQPRL EGEEYLEIID EFMEAAFTRW PKAVVQFEDF QAKWAFGTLE RYRKKFCMFN
301: DDVQGTAGVA LAGLLGTVRA QGRPISDFVN QKIVVVGAGS AGLGVTKMAV QAVARMAGIS ESEATKNFYL IDKDGLVTTE RTKLDPGAVL FAKNPAEIRE
401: GASIVEVVKK VRPHVLLGLS GVGGIFNEEV LKAMRESDSC KPAIFAMSNP TLNAECTAAD AFKHAGGNIV FASGSPFENV ELENGKVGHV NQANNMYLFP
501: GIGLGTLLSG ARIVTDGMLQ AASECLASYM TDEEVQKGIL YPSINNIRHI TAEVGAAVLR AAVTDDIAEG HGDVGPKDLS HMSKEDTVNY ITRNMWFPVY
601: SPLVHEK

Arabidopsis Description

NAD-ME2NAD-dependent malic enzyme 2, mitochondrial [Source:UniProtKB/Swiss-Prot;Acc:Q8L7K9]
SUBAcon: [mitochondrion]

Hydropathy Plot

About CropPAL

The Protein Annotated Locations Database (CropPAL) houses large scale proteomic and GFP localization data from published experimental studies in Soybean (Glycine max), Maize (Zea mays), Wheat (Triticum aestivum), Barley (Hordeum vulgare), Rice (Oryza sativa), Field mustard (Brassica rapa), Canola (Brassica napus), Sorghum (Sorghum bicolor), Potato (Solanum tuberosum), Tomato (Solanum lycopersicum), Banana (Musa acuminata) and Wine grape (Vitis vinifera) as well as precomputed predictions for protein subcellular localizations using protein sequences.