Factsheet for Os01t0188400-01

Subcellular Localization

min:

: max

Winner_takes_all: plastid

Predictor Summary:

plastid 7
mitochondrion 3

Predictors

GFP

MS/MS

Papers

mitochondrion: 15069638
plastid: 17189339
plasma membrane: 27800704

msms PMID: 27800704 doi

J Cao, C Yang, L Li, L Jiang, Y Wu, C Wu, Q Bu, G Xia, X Liu, Y Luo, J Liu

Huai'an Institute of Agricultural Sciences, Huai'an, 223001, China., Institute of Analytical Chemistry and Synthetic and Functional Biomolecules Center, College of Chemistry and Molecular Engineering, Peking University, Beijing, 100871, China; and., Northeast Institute of Geography and Agroecology, Key Laboratory of Soybean Molecular Design Breeding, Chinese Academy of Sciences, Harbin, 150081, China., State Key Laboratory of Plant Genomics, Institute of Microbiology, Chinese Academy of Sciences, Beijing, 100101, China.

msms PMID: 15069638 doi

N Tanaka, M Fujita, H Handa, S Murayama, M Uemura, Y Kawamura, T Mitsui, S Mikami, Y Tozawa, T Yoshinaga, S Komatsu

Department of Molecular Genetics, National Institute of Agrobiological Sciences, 305-8602, Tsukuba, Japan.

msms PMID: 17189339 doi

T Kleffmann, A von Zychlinski, D Russenberger, M Hirsch-Hoffmann, P Gehrig, W Gruissem, S Baginsky

Institute of Plant Sciences, Eidgenössische Technische Hochschule Zurich, 8092 Zurich, Switzerland.

PPI

Inferred distinct locusB in Crop

locusB	locations
Os01t0218200-00
Os05t0210600-01
Os05t0587300-01

Inferred from Arabidopsis experimental PPI

Ath locusA	locusB	Ath locusB	Paper
AT5G11670.1	Os01t0218200-00	AT2G42580.1	19000166
AT5G11670.1	Os05t0210600-01	AT2G42580.1	19000166
AT5G11670.1	Os05t0587300-01	AT2G42580.1	19000166

Homology

Paralog

locus	Identity	Homology Identity

Ortholog

locus	Homology Species	Location	Identity	Homology Identity
TraesCS3B01G128000.1	Wheat	cytosol	84.19	91.03
TraesCS3D01G110700.1	Wheat	plastid	90.92	90.5
TraesCS3A01G108900.1	Wheat	golgi	90.77	89.51
KXG21798	Sorghum	plastid	87.32	85.58
Zm00001d037961_P004	Maize	plastid	86.07	85.4
Zm00001d037962_P003	Maize	plastid	85.76	85.09
Os05t0186300-01	Rice	cytosol, plasma membrane	75.9	85.09
VIT_04s0008g00180.t01	Wine grape	cytosol	74.65	81.54
Zm00001d000316_P001	Maize	plastid	76.53	81.09
EES00150	Sorghum	plastid	79.81	80.19
HORVU3Hr1G019790.9	Barley	plastid	90.14	79.01
Solyc08g066360.2.1	Tomato	nucleus, plastid, unclear	71.83	77.67
CDX91199	Canola	cytosol	69.95	76.02
CDY61446	Canola	cytosol	69.8	75.85
Bra023336.1-P	Field mustard	cytosol	69.8	75.85
CDX88203	Canola	cytosol	69.33	75.34
Bra009866.1-P	Field mustard	cytosol	69.33	75.34
KRH52781	Soybean	mitochondrion	72.93	75.28
CDX80544	Canola	cytosol	69.17	75.17
Bra006107.1-P	Field mustard	cytosol	69.17	75.17
CDX78432	Canola	cytosol	68.86	74.83
CDX70409	Canola	cytosol	68.86	74.83
KRH62099	Soybean	mitochondrion	72.77	74.76
Os01t0743500-01	Rice	plasma membrane, plastid	68.23	74.53
AT5G11670.1	Thale cress	cytosol	68.54	74.49
AT5G25880.1	Thale cress	cytosol	68.39	74.32
VIT_02s0012g02460.t01	Wine grape	endoplasmic reticulum, plasma membrane	49.77	73.61
Os01t0723400-04	Rice	plastid	70.89	70.45
OQU86165	Sorghum	mitochondrion	73.71	66.62
Os02t0665000-01	Rice	cytosol	18.78	64.52
CDY02046	Canola	mitochondrion	55.4	63.33
PGSC0003DMT400008389	Potato	mitochondrion	21.28	51.32
Os10t0503500-01	Rice	mitochondrion	37.25	38.39
Os07t0496200-01	Rice	mitochondrion	36.93	37.94

Protein Annotations

Gene3D:3.40.50.10380	Gene3D:3.40.50.720	EntrezGene:4326769	MapMan:5.1.1.4	EMBL:AK121770	ProteinID:BAB03427.1
ProteinID:BAB07934.1	ProteinID:BAF04171.1	ProteinID:BAS70801.1	EMBL:D16499	ProteinID:EEE54023.1	GO:GO:0000166
GO:GO:0003674	GO:GO:0003824	GO:GO:0004470	GO:GO:0004471	GO:GO:0004473	GO:GO:0005488
GO:GO:0005575	GO:GO:0005622	GO:GO:0005623	GO:GO:0005737	GO:GO:0006090	GO:GO:0006108
GO:GO:0008150	GO:GO:0008152	GO:GO:0008948	GO:GO:0009507	GO:GO:0009536	GO:GO:0009987
GO:GO:0016491	GO:GO:0046872	GO:GO:0051287	GO:GO:0055114	InterPro:IPR037062	InterPro:Malic_NAD-bd
InterPro:Malic_N_dom	InterPro:Malic_N_dom_sf	InterPro:Malic_OxRdtase	InterPro:Malic_enzyme_CS	InterPro:NAD(P)-bd_dom_sf	EnsemblPlantsGene:Os01g0188400
EnsemblPlants:Os01t0188400-01	UniProt:P43279	PFAM:PF00390	PFAM:PF03949	PIRSF:PIRSF000106	PRINTS:PR00072
ScanProsite:PS00331	PANTHER:PTHR23406	PANTHER:PTHR23406:SF29	SMART:SM00919	SMART:SM01274	SUPFAM:SSF51735
SUPFAM:SSF53223	UniParc:UPI000009EED9	RefSeq:XP_015621553.1	SEG:seg	:	:

Description

NADP-MALIC ENZYME 1NADP-dependent malic enzyme, chloroplast precursor (EC 1.1.1.40) (NADP-ME). (Os01t0188400-01);Similar to Malic enzyme. (Os01t0188400-02)

Coordinates

chr1:-:4739271..4744472

Molecular Weight (calculated)

69869.8 Da

IEP (calculated)

7.155

GRAVY (calculated)

-0.161

Length

639 amino acids

Sequence
(BLAST)

001: MLSARAAATA AAAAASPLWK RGEGGSSGSG SGCTSCREVR RRAAAVRVRA AAPRRVEAVA MESAAETEKK EEVAAAGGGV EDMATEEVPV TPWAFSVASG
101: YTLLRDPHHN KGLAFSEKER DAHYLRGLLP PAVVSQDLQV KKIMHNLRQY SVPLQRYMAM MDLQERNERL FYKLLIDNVE ELLPVVYTPT VGEACQKYGS
201: IFRQPQGLYV SLKDKGKVLD VLRNWPERNI QVIVVTDGER ILGLGDLGCQ GMGIPVGKLS LYTALGGVRP SACLPITIDV GTNNEQLLND EFYIGLRQRR
301: ATGKEYHELM EEFMSAVKQI YGEKVLIQFE DFANHNAFDL LAKYSKSHLV FNDDIQGTAS VVLAGLLSSL KVVGGTLAEH TYLFLGAGEA GTGIAELIAL
401: EISKQTKAPI EECRKKVWLL DSKGLIVNSR KESLQAFKKP WAHEHEPVTT LLDAVQSIKP TVLIGTSGVG KTFTKEVIEA MASFNERPVI FSLSNPTSHS
501: ECTAEEAYNW SQGRAVFASG SPFDPVEYNG KIHVPGQSNN AYIFPGFGLG VVISGAVRVH EDMLLAASET LADQATQENF EKGSIFPPFT NIRKISARIA
601: ASVAAKAYEL GLATRLPQPR DLEKYAESCM YTPVYRSYR

Best Arabidopsis Sequence Match ( AT1G79750.1 )
(BLAST)

001: MISLTPSLFL NKTVVPGCST RLSLRQPRTI ITPPASLRVF SSLGSNQDPT GSVLIETTAT SSSSLETSAA DIVPKSTVSG GVQDVYGEDA ATEDMPITPW
101: SLSVASGYTL LRDPHHNKGL AFSHRERDAH YLRGLLPPTV ISQDLQVKKI MHTLRQYQVP LQKYMAMMDL QETNERLFYK LLIDHVEELL PVIYTPTVGE
201: ACQKYGSIFL RPQGLFISLK EKGKIHEVLR NWPEKNIQVI VVTDGERILG LGDLGCQGMG IPVGKLSLYT ALGGVRPSAC LPVTIDVGTN NEKLLNDEFY
301: IGLRQRRATG EEYSELMHEF MTAVKQNYGE KVVIQFEDFA NHNAFDLLAK YGTTHLVFND DIQGTASVVL AGLIAALRFV GGSLSDHRFL FLGAGEAGTG
401: IAELIALEIS KKSHIPLEEA RKNIWLVDSK GLIVSSRKES IQHFKKPWAH DHEPIRELVD AVKAIKPTVL IGTSGVGQTF TQDVVETMAK LNEKPIILSL
501: SNPTSQSECT AEEAYTWSQG RAIFASGSPF APVEYEGKTF VPGQANNAYI FPGFGLGLIM SGTIRVHDDM LLAASEALAE ELMEEHYEKG MIYPPFRNIR
601: KISARIAAKV AAKAYELGLA TRLPQPKELE QCAESSMYSP SYRSYR

Arabidopsis Description

NADP-ME4Malic enzyme [Source:UniProtKB/TrEMBL;Acc:A0A178W4H6]
SUBAcon: [plastid]

Hydropathy Plot

About CropPAL

The Protein Annotated Locations Database (CropPAL) houses large scale proteomic and GFP localization data from published experimental studies in Soybean (Glycine max), Maize (Zea mays), Wheat (Triticum aestivum), Barley (Hordeum vulgare), Rice (Oryza sativa), Field mustard (Brassica rapa), Canola (Brassica napus), Sorghum (Sorghum bicolor), Potato (Solanum tuberosum), Tomato (Solanum lycopersicum), Banana (Musa acuminata) and Wine grape (Vitis vinifera) as well as precomputed predictions for protein subcellular localizations using protein sequences.