Factsheet for Zm00001d000316_P001

Subcellular Localization

min:

: max

Winner_takes_all: plastid

Predictor Summary:

plastid 5
mitochondrion 2
cytosol 1

Predictors

GFP

MS/MS

Papers

plastid: 22065420
extracellular: 22192489
plastid: 23198870
cytosol: 28250383

msms PMID: 28250383 doi

CN Meisrimler, F Buck, S Lüthje

University Hospital Eppendorf, Institute of Clinical Chemistry, Campus Science, Martinistraße 52, D-20246 Hamburg, Germany. buck@uke.de., University of Hamburg, Biocenter Klein Flottbek and Botanical Garden, Oxidative Stress and Plant Proteomics Group, Ohnhorststraße 18, D-22609 Hamburg, Germany. c_m_2406@yahoo.de., University of Hamburg, Biocenter Klein Flottbek and Botanical Garden, Oxidative Stress and Plant Proteomics Group, Ohnhorststraße 18, D-22609 Hamburg, Germany. s.luthje@botanik.uni-hamburg.de.

msms PMID: 22192489 doi

K Witzel, M Shahzad, A Matros, HP Mock, KH Mühling

Institute of Plant Nutrition and Soil Science, Christian Albrechts University, Hermann-Rodewald-Strasse 2, 24118 Kiel, Germany. khmuehling@plantnutrition.uni-kiel.de.

msms PMID: 23198870 doi

M Huang, G Friso, K Nishimura, X Qu, PD Olinares, W Majeran, Q Sun, KJ van Wijk

Department of Plant Biology, Cornell University, Ithaca, New York 14853, United States.

msms PMID: 22065420 doi

W Majeran, G Friso, Y Asakura, X Qu, M Huang, L Ponnala, KP Watkins, A Barkan, KJ van Wijk

Department of Plant Biology, Cornell University, Ithaca, New York 14853, USA.

PPI

Inferred distinct locusB in Crop

locusB	locations
Zm00001d004494_P001
Zm00001d006366_P001
Zm00001d008930_P003
Zm00001d009724_P009
Zm00001d011352_P001
Zm00001d033961_P001
Zm00001d039362_P002
Zm00001d044479_P001

Inferred from Arabidopsis experimental PPI

Ath locusA	locusB	Ath locusB	Paper
AT5G11670.1	Zm00001d004494_P001	AT2G42580.1	19000166
AT5G11670.1	Zm00001d006366_P001	AT2G42580.1	19000166
AT5G11670.1	Zm00001d008930_P003	AT2G42580.1	19000166
AT5G11670.1	Zm00001d009724_P009	AT2G42580.1	19000166
AT5G11670.1	Zm00001d011352_P001	AT2G42580.1	19000166
AT5G11670.1	Zm00001d033961_P001	AT2G42580.1	19000166
AT5G11670.1	Zm00001d039362_P002	AT2G42580.1	19000166
AT5G11670.1	Zm00001d044479_P001	AT2G42580.1	19000166

Homology

Paralog

locus	Identity	Homology Identity

Ortholog

locus	Homology Species	Location	Identity	Homology Identity
EES00150	Sorghum	plastid	89.05	84.43
Zm00001d037961_P004	Maize	plastid	85.9	80.43
Zm00001d037962_P003	Maize	plastid	85.57	80.12
TraesCS3B01G128000.1	Wheat	cytosol	75.46	76.99
Os01t0188400-01	Rice	plastid	81.09	76.53
TraesCS3D01G110700.1	Wheat	plastid	80.93	76.01
TraesCS3A01G108900.1	Wheat	golgi	80.93	75.31
Zm00001d037693_P003	Maize	extracellular	70.48	74.56
Zm00001d010358_P001	Maize	cytosol	70.15	74.21
VIT_04s0008g00180.t01	Wine grape	cytosol	70.15	72.31
Zm00001d012764_P001	Maize	cytosol	58.71	71.23
Solyc08g066360.2.1	Tomato	nucleus, plastid, unclear	68.66	70.05
CDX88203	Canola	cytosol	67.5	69.22
Bra009866.1-P	Field mustard	cytosol	67.5	69.22
CDX80544	Canola	cytosol	67.33	69.05
CDX91199	Canola	cytosol	66.83	68.54
Bra023336.1-P	Field mustard	cytosol	66.67	68.37
CDY61446	Canola	cytosol	66.67	68.37
Zm00001d036873_P001	Maize	cytosol	5.64	68.0
AT5G25880.1	Thale cress	cytosol	66.0	67.69
KRH52781	Soybean	mitochondrion	69.32	67.53
CDX70409	Canola	cytosol	65.84	67.52
AT5G11670.1	Thale cress	cytosol	65.84	67.52
CDX78432	Canola	cytosol	65.67	67.35
Bra006107.1-P	Field mustard	cytosol	65.67	67.35
KRH62099	Soybean	mitochondrion	68.82	66.72
HORVU3Hr1G019790.9	Barley	plastid	79.27	65.57
Zm00001d043601_P003	Maize	mitochondrion	69.98	63.55
VIT_02s0012g02460.t01	Wine grape	endoplasmic reticulum, plasma membrane	45.44	63.43
CDY02046	Canola	mitochondrion	54.23	58.5
Zm00001d034292_P001	Maize	cytosol	24.71	54.38
Zm00001d021546_P003	Maize	mitochondrion	37.81	36.83
Zm00001d013911_P001	Maize	mitochondrion	36.98	34.84
Zm00001d003090_P001	Maize	mitochondrion	15.42	27.27
Zm00001d042392_P001	Maize	plastid	11.11	25.97
Zm00001d051182_P001	Maize	cytosol	7.13	19.46

Protein Annotations

Gene3D:3.40.50.10380	Gene3D:3.40.50.720	MapMan:5.1.1.4	UniProt:A0A1X7YHV0	ncoils:Coil	GO:GO:0000166
GO:GO:0003674	GO:GO:0003824	GO:GO:0004470	GO:GO:0004471	GO:GO:0004473	GO:GO:0005488
GO:GO:0005575	GO:GO:0005622	GO:GO:0005623	GO:GO:0005737	GO:GO:0008150	GO:GO:0008152
GO:GO:0008948	GO:GO:0009507	GO:GO:0009536	GO:GO:0016491	GO:GO:0046872	GO:GO:0051287
GO:GO:0055114	InterPro:IPR037062	InterPro:Malic_NAD-bd	InterPro:Malic_N_dom	InterPro:Malic_N_dom_sf	InterPro:Malic_OxRdtase
InterPro:Malic_enzyme_CS	InterPro:NAD(P)-bd_dom_sf	PFAM:PF00390	PFAM:PF03949	PIRSF:PIRSF000106	PRINTS:PR00072
ScanProsite:PS00331	PANTHER:PTHR23406	PANTHER:PTHR23406:SF29	SMART:SM00919	SMART:SM01274	SUPFAM:SSF51735
SUPFAM:SSF53223	UniParc:UPI000843B09C	EnsemblPlantsGene:Zm00001d000316	EnsemblPlants:Zm00001d000316_P001	EnsemblPlants:Zm00001d000316_T001	SEG:seg

Description

NADP malic enzyme3NADP-dependent malic enzyme%2C chloroplastic

Coordinates

chrB73V4_ctg89:+:18903..24123

Molecular Weight (calculated)

66051.2 Da

IEP (calculated)

6.271

GRAVY (calculated)

-0.139

Length

603 amino acids

Sequence
(BLAST)

001: MLSTRTAAVA ASASPASPWK LGGRSEGGAS CDGCRTYRNT LRRRAAPAKV RALPPRRVDA VAMVSNAETE TEKEQEEAAA ASEELPVMPW ATSVASGYTL
101: LRDPHHNKGL AFTEEERDGH YLRGLLPPAV LSQELQIKKF MNTLRQYQTP LQRYIAMMNL QETDERLFYK LLIDNVVELL PFVYTPTVGE ACQKYGSIFG
201: RPQGLYVSLK DKGKVLEVLR NWPHRNIQVI CVTDGERILG LGDLGCQGMG IPVGKLALYT ALGGVDPSVC LPITIDVGTN NEKLLNDEFY IGLRQKRATG
301: EEYDELIEEF MSAVKQFYGE KVLIQFEDFA NHNAFDLLEK YSKSHLVFND DIQGTASVVL AGLLAALKMV GGTLAEQTYL FLGAGEAGTG IAELIALEIS
401: KQTNAPIEEC RKKVWLVDSK SIKPTVLIGT SGVGRTFTKE IIEAMSSFNE RPIIFSLSNP TSHSECTAEQ AYTWSQGRSI FASGSPFAPV EYEGKTFVPG
501: QSNNAYIFPG LGLGLVISGA VRVHEDMLLA ASKALADQAT QDNFEKGSIF PPFTSIRKIS AHIAAAVAAK AYELGLATRL PPPSDLVKYA ENCMYTPVYR
601: NYR

Best Arabidopsis Sequence Match ( AT1G79750.1 )
(BLAST)

001: MISLTPSLFL NKTVVPGCST RLSLRQPRTI ITPPASLRVF SSLGSNQDPT GSVLIETTAT SSSSLETSAA DIVPKSTVSG GVQDVYGEDA ATEDMPITPW
101: SLSVASGYTL LRDPHHNKGL AFSHRERDAH YLRGLLPPTV ISQDLQVKKI MHTLRQYQVP LQKYMAMMDL QETNERLFYK LLIDHVEELL PVIYTPTVGE
201: ACQKYGSIFL RPQGLFISLK EKGKIHEVLR NWPEKNIQVI VVTDGERILG LGDLGCQGMG IPVGKLSLYT ALGGVRPSAC LPVTIDVGTN NEKLLNDEFY
301: IGLRQRRATG EEYSELMHEF MTAVKQNYGE KVVIQFEDFA NHNAFDLLAK YGTTHLVFND DIQGTASVVL AGLIAALRFV GGSLSDHRFL FLGAGEAGTG
401: IAELIALEIS KKSHIPLEEA RKNIWLVDSK GLIVSSRKES IQHFKKPWAH DHEPIRELVD AVKAIKPTVL IGTSGVGQTF TQDVVETMAK LNEKPIILSL
501: SNPTSQSECT AEEAYTWSQG RAIFASGSPF APVEYEGKTF VPGQANNAYI FPGFGLGLIM SGTIRVHDDM LLAASEALAE ELMEEHYEKG MIYPPFRNIR
601: KISARIAAKV AAKAYELGLA TRLPQPKELE QCAESSMYSP SYRSYR

Arabidopsis Description

NADP-ME4Malic enzyme [Source:UniProtKB/TrEMBL;Acc:A0A178W4H6]
SUBAcon: [plastid]

Hydropathy Plot

About CropPAL

The Protein Annotated Locations Database (CropPAL) houses large scale proteomic and GFP localization data from published experimental studies in Soybean (Glycine max), Maize (Zea mays), Wheat (Triticum aestivum), Barley (Hordeum vulgare), Rice (Oryza sativa), Field mustard (Brassica rapa), Canola (Brassica napus), Sorghum (Sorghum bicolor), Potato (Solanum tuberosum), Tomato (Solanum lycopersicum), Banana (Musa acuminata) and Wine grape (Vitis vinifera) as well as precomputed predictions for protein subcellular localizations using protein sequences.