Factsheet for Bra023336.1-P

Subcellular Localization

min:

: max

Winner_takes_all: cytosol

Predictor Summary:

cytosol 3
plastid 2
mitochondrion 1
peroxisome 1

Predictors

GFP

MS/MS

Papers

PPI

Inferred distinct locusB in Crop

locusB	locations
Bra000267.1-P
Bra004709.1-P

Inferred from Arabidopsis experimental PPI

Ath locusA	locusB	Ath locusB	Paper
AT5G11670.1	Bra000267.1-P	AT2G42580.1	19000166
AT5G11670.1	Bra004709.1-P	AT2G42580.1	19000166

Homology

Paralog

locus	Identity	Homology Identity

Ortholog

locus	Homology Species	Location	Identity	Homology Identity
CDY61446	Canola	cytosol	100.0	100.0
Bra006107.1-P	Field mustard	cytosol	95.58	95.58
AT5G11670.1	Thale cress	cytosol	94.73	94.73
Bra009866.1-P	Field mustard	cytosol	91.84	91.84
Os05t0186300-01	Rice	cytosol, plasma membrane	80.1	82.63
VIT_04s0008g00180.t01	Wine grape	cytosol	80.95	81.37
Zm00001d037693_P003	Maize	extracellular	78.06	80.53
EES19164	Sorghum	cytosol, peroxisome, plastid	77.89	80.35
Solyc08g066360.2.1	Tomato	nucleus, plastid, unclear	79.59	79.19
Zm00001d010358_P001	Maize	cytosol	76.7	79.12
TraesCS1A01G122500.1	Wheat	cytosol	76.7	79.12
PGSC0003DMT400077452	Potato	cytosol	79.42	79.02
TraesCS1B01G141700.1	Wheat	cytosol	76.53	78.95
KRH52781	Soybean	mitochondrion	81.12	77.06
KRH62099	Soybean	mitochondrion	81.46	77.01
TraesCS3B01G128000.1	Wheat	cytosol	76.36	75.97
TraesCS1D01G123400.2	Wheat	mitochondrion	73.47	75.39
Bra039029.1-P	Field mustard	cytosol	74.15	74.91
HORVU1Hr1G028030.16	Barley	mitochondrion	75.34	72.15
Zm00001d036873_P001	Maize	cytosol	6.12	72.0
VIT_02s0012g02460.t01	Wine grape	endoplasmic reticulum, plasma membrane	52.55	71.53
Zm00001d037961_P004	Maize	plastid	76.7	70.03
Os01t0188400-01	Rice	plastid	75.85	69.8
Zm00001d037962_P003	Maize	plastid	76.36	69.72
TraesCS3D01G110700.1	Wheat	plastid	76.02	69.63
TraesCS3A01G108900.1	Wheat	golgi	76.19	69.14
KXG21798	Sorghum	plastid	76.36	68.86
Bra003738.1-P	Field mustard	plastid	73.3	67.87
EES00150	Sorghum	plastid	73.13	67.61
Zm00001d000316_P001	Maize	plastid	68.37	66.67
Os02t0665000-01	Rice	cytosol	20.24	63.98
Zm00001d034292_P001	Maize	cytosol	28.57	61.31
HORVU3Hr1G019790.9	Barley	plastid	75.17	60.63
OQU86165	Sorghum	mitochondrion	66.16	55.02
HORVU5Hr1G018620.1	Barley	cytosol	9.18	46.55
Bra037210.1-P	Field mustard	mitochondrion	40.99	39.0
Bra037347.1-P	Field mustard	mitochondrion	37.07	37.14
Bra000967.1-P	Field mustard	mitochondrion	38.27	36.29
HORVU3Hr1G102340.13	Barley	plastid	11.05	27.08
Zm00001d003090_P001	Maize	mitochondrion	14.8	25.51
Zm00001d051182_P001	Maize	cytosol	6.63	17.65

Protein Annotations

Gene3D:3.40.50.10380	Gene3D:3.40.50.720	MapMan:5.1.1.4	EnsemblPlantsGene:Bra023336	EnsemblPlants:Bra023336.1	EnsemblPlants:Bra023336.1-P
GO:GO:0000166	GO:GO:0003674	GO:GO:0003824	GO:GO:0004470	GO:GO:0004471	GO:GO:0005488
GO:GO:0008150	GO:GO:0008152	GO:GO:0016491	GO:GO:0046872	GO:GO:0051287	GO:GO:0055114
InterPro:IPR037062	UniProt:M4E3I4	InterPro:Malic_NAD-bd	InterPro:Malic_N_dom	InterPro:Malic_N_dom_sf	InterPro:Malic_OxRdtase
InterPro:Malic_enzyme_CS	InterPro:NAD(P)-bd_dom_sf	PFAM:PF00390	PFAM:PF03949	PIRSF:PIRSF000106	PRINTS:PR00072
ScanProsite:PS00331	PANTHER:PTHR23406	PANTHER:PTHR23406:SF36	SMART:SM00919	SMART:SM01274	SUPFAM:SSF51735
SUPFAM:SSF53223	UniParc:UPI0002543398	:	:	:	:

Description

AT5G11670 (E=0.0) ATNADP-ME2 | ATNADP-ME2 (NADP-malic enzyme 2); malate dehydrogenase (oxaloacetate-decarboxylating) (NADP+)/ malic enzyme/ oxidoreductase, acting on NADH or NADPH, NAD or NADP as acceptor

Coordinates

chrA02:+:2914123..2917727

Molecular Weight (calculated)

64483.5 Da

IEP (calculated)

5.722

GRAVY (calculated)

-0.099

Length

588 amino acids

Sequence
(BLAST)

001: MGSTPAEVPC DVVSDNRSGL GGGISDVYGE DLATLDQLVT PWVSSVASGY SLMRDPRYNK GLAFTDKERD AHYLTGLLPP VVLSQEVQER KLMHNLRQYT
101: VPLQRYMALM DLQERNERLF YKLLIDNVEE LLPVVYTPTV GEACQKYGSI FRKPQGLYIS LKEKGRILEV LKNWPQRGIQ VIVVTDGERI LGLGDLGCQG
201: MGIPVGKLSL YTALGGIRPS ACLPITIDVG TNNEKLLNDE FYIGLKQRRA TGQEYAEFLH EFMCAVKQNY GEKVLVQFED FANHNAFDLL SKYSTSHLVF
301: NDDIQGTASV VLAGLIAAQK VLGKSLADHT FLFLGAGEAG TGIAELIALK ISKETGAPID ETRKKIWLVD SKGLIVSSRK ESLQHFKQPW AHEHEPVKNL
401: IGAVNAIKPT VLIGTSGVGQ TFTQEVVEAM ATNNETPLIL ALSNPTSQAE CTAEQAYTWT KGRAIFGSGS PFDPVEYDGK TYFPGQANNC YIFPGLGLGL
501: IMSGAIRVRD DMLLAASEAL AAQVTEENFA NGLIYPPFSN IREISANIAA SVAAKTYELG LASNLPRPKD LVKFAESCMY SPVYRNYR

Best Arabidopsis Sequence Match ( AT5G11670.1 )
(BLAST)

001: MGSTPTDLPG EDVADNRSGV GGGISDVYGE DSATLDQLVT PWVTSVASGY TLMRDPRYNK GLAFTDKERD AHYLTGLLPP VILSQDVQER KVMHNLRQYT
101: VPLQRYMALM DLQERNERLF YKLLIDNVEE LLPVVYTPTV GEACQKYGSI FRKPQGLYIS LNEKGKILEV LKNWPQRGIQ VIVVTDGERI LGLGDLGCQG
201: MGIPVGKLSL YTALGGIRPS ACLPITIDVG TNNEKLLNDE FYIGLKQRRA TGQEYAEFLH EFMCAVKQNY GEKVLVQFED FANHNAFDLL SKYSDSHLVF
301: NDDIQGTASV VLAGLIAAQK VLGKKLADHT FLFLGAGEAG TGIAELIALK ISKETGAPIT ETRKKIWLVD SKGLIVSSRK ESLQHFKQPW AHEHKPVKDL
401: IGAVNAIKPT VLIGTSGVGQ TFTKEVVEAM ATNNEKPLIL ALSNPTSQAE CTAEQAYTWT KGRAIFGSGS PFDPVVYDGK TYLPGQANNC YIFPGLGLGL
501: IMSGAIRVRD DMLLAASEAL AAQVTEEHYA NGLIYPPFSN IREISANIAA CVAAKTYDLG LASNLPRAKD LVKFAESSMY SPVYRNYR

Arabidopsis Description

NADP-ME2NADP-dependent malic enzyme 2 [Source:UniProtKB/Swiss-Prot;Acc:Q9LYG3]
SUBAcon: [cytosol]

Hydropathy Plot

About CropPAL

The Protein Annotated Locations Database (CropPAL) houses large scale proteomic and GFP localization data from published experimental studies in Soybean (Glycine max), Maize (Zea mays), Wheat (Triticum aestivum), Barley (Hordeum vulgare), Rice (Oryza sativa), Field mustard (Brassica rapa), Canola (Brassica napus), Sorghum (Sorghum bicolor), Potato (Solanum tuberosum), Tomato (Solanum lycopersicum), Banana (Musa acuminata) and Wine grape (Vitis vinifera) as well as precomputed predictions for protein subcellular localizations using protein sequences.