Factsheet for Solyc08g066360.2.1

Subcellular Localization

min:

: max

Winner_takes_all: unclear, plastid, nucleus

Predictor Summary:

cytosol 3
plastid 2
mitochondrion 1
plasma membrane 1

Predictors

GFP

MS/MS

Papers

plastid: 22908117
unclear: 26455813
nucleus: 28394025

msms PMID: 28394025 doi

AJM Howden, R Stam, V Martinez Heredia, GB Motion, S Ten Have, K Hodge, TM Marques Monteiro Amaro, E Huitema

Cell and Molecular Sciences, JHI, Invergowrie, Dundee, DD2 5DA, UK., Division of Plant Science, School of Life Sciences, University of Dundee at the James Hutton Institute (JHI), Invergowrie, Dundee, DD2 5DA, UK., Dundee Effector Consortium, JHI, Invergowrie, Dundee, DD2 5DA, UK., Section of Population Genetics, Technische Universität München, 85354, Freising, Germany., Wellcome Trust Centre for Gene Regulation and Expression, School of Life Sciences, University of Dundee, Dow Street, Dundee, DD1 5EH, UK.

msms PMID: 22908117 doi

C Barsan, M Zouine, E Maza, W Bian, I Egea, M Rossignol, D Bouyssie, C Pichereaux, E Purgatto, M Bouzayen, A Latché, JC Pech

Université de Toulouse, Institut National Polytechnique-Ecole Nationale Supérieure Agronomique de Toulouse, Génomique et Biotechnologie des Fruits, Castanet-Tolosan F-31326, France.

msms PMID: 26455813 doi

P Paul, P Chaturvedi, M Selymesi, A Ghatak, A Mesihovic, KD Scharf, W Weckwerth, S Simm, E Schleiff

Goethe University, Department of Biosciences, Molecular Cell Biology of Plants, Frankfurt/Main, Germany., Goethe University, Department of Biosciences, Molecular Cell Biology of Plants, Frankfurt/Main, Germany; SPOT-ITN Consortium., Goethe University, Department of Biosciences, Molecular Cell Biology of Plants, Frankfurt/Main, Germany; SPOT-ITN Consortium; Goethe University, Cluster of Excellence Frankfurt, Frankfurt/Main, Germany; Goethe University, Buchmann Institute of Molecular Life Sciences, Frankfurt/Main, Germany. Electronic address: schleiff@bio.uni-frankfurt.de., SPOT-ITN Consortium; University of Vienna, Department of Ecogenomics and Systems biology, Vienna, Austria., University of Vienna, Department of Ecogenomics and Systems biology, Vienna, Austria; School of Biotechnology and Bioinformatics, D.Y. Patil University, Mumbai, India.

PPI

No PPI Data

Homology

Paralog

locus	Identity	Homology Identity

Ortholog

locus	Homology Species	Location	Identity	Homology Identity
PGSC0003DMT400077452	Potato	cytosol	98.98	98.98
VIT_04s0008g00180.t01	Wine grape	cytosol	84.43	85.3
Os05t0186300-01	Rice	cytosol, plasma membrane	80.03	82.98
Solyc05g050120.2.1	Tomato	plastid	80.71	82.38
Zm00001d037693_P003	Maize	extracellular	78.17	81.05
TraesCS1B01G141700.1	Wheat	cytosol	78.17	81.05
TraesCS1A01G122500.1	Wheat	cytosol	78.0	80.88
Zm00001d010358_P001	Maize	cytosol	77.5	80.35
EES19164	Sorghum	cytosol, peroxisome, plastid	77.5	80.35
KRH52781	Soybean	mitochondrion	83.93	80.13
CDX88203	Canola	cytosol	79.53	79.93
CDX91199	Canola	cytosol	79.53	79.93
Bra009866.1-P	Field mustard	cytosol	79.53	79.93
CDX80544	Canola	cytosol	79.36	79.76
KRH62099	Soybean	mitochondrion	83.93	79.74
CDX78432	Canola	cytosol	79.19	79.59
CDY61446	Canola	cytosol	79.19	79.59
Bra023336.1-P	Field mustard	cytosol	79.19	79.59
Bra006107.1-P	Field mustard	cytosol	79.19	79.59
AT5G25880.1	Thale cress	cytosol	78.85	79.25
CDX70409	Canola	cytosol	78.85	79.25
AT5G11670.1	Thale cress	cytosol	78.68	79.08
Solyc12g008430.1.1	Tomato	plastid	77.83	78.1
TraesCS3B01G128000.1	Wheat	cytosol	77.5	77.5
TraesCS1D01G123400.2	Wheat	mitochondrion	74.45	76.79
Solyc03g120990.2.1	Tomato	plastid	81.05	74.61
VIT_02s0012g02460.t01	Wine grape	endoplasmic reticulum, plasma membrane	54.15	74.07
Solyc12g044600.2.1	Tomato	plastid	79.19	73.12
HORVU1Hr1G028030.16	Barley	mitochondrion	75.3	72.48
Os01t0188400-01	Rice	plastid	77.67	71.83
Zm00001d037961_P004	Maize	plastid	77.67	71.27
TraesCS3D01G110700.1	Wheat	plastid	77.33	71.18
Zm00001d037962_P003	Maize	plastid	77.33	70.96
TraesCS3A01G108900.1	Wheat	golgi	77.33	70.52
KXG21798	Sorghum	plastid	77.5	70.25
Zm00001d036873_P001	Maize	cytosol	5.92	70.0
Zm00001d000316_P001	Maize	plastid	70.05	68.66
CDY02046	Canola	mitochondrion	63.28	66.91
EES00150	Sorghum	plastid	71.91	66.82
Solyc08g066290.2.1	Tomato	cytosol	8.12	66.67
Os02t0665000-01	Rice	cytosol	20.98	66.67
HORVU3Hr1G019790.9	Barley	plastid	76.48	62.0
Zm00001d034292_P001	Maize	cytosol	27.58	59.49
OQU86165	Sorghum	mitochondrion	66.5	55.59
HORVU5Hr1G018620.1	Barley	cytosol	8.29	42.24
Solyc01g094200.2.1	Tomato	plastid	39.93	39.27
Solyc08g013860.2.1	Tomato	plastid	40.95	38.66
Zm00001d003090_P001	Maize	mitochondrion	15.74	27.27
HORVU3Hr1G102340.13	Barley	plastid	10.83	26.67
Zm00001d051182_P001	Maize	cytosol	7.28	19.46

Protein Annotations

Gene3D:3.40.50.10380	Gene3D:3.40.50.720	MapMan:5.1.1.4	GO:GO:0000166	GO:GO:0003674	GO:GO:0003824
GO:GO:0004470	GO:GO:0004471	GO:GO:0004473	GO:GO:0005488	GO:GO:0005575	GO:GO:0005622
GO:GO:0005623	GO:GO:0005737	GO:GO:0006090	GO:GO:0006108	GO:GO:0008150	GO:GO:0008152
GO:GO:0009507	GO:GO:0009536	GO:GO:0009987	GO:GO:0016491	GO:GO:0046872	GO:GO:0051287
GO:GO:0055114	InterPro:IPR037062	UniProt:K4CLL3	InterPro:Malic_NAD-bd	InterPro:Malic_N_dom	InterPro:Malic_N_dom_sf
InterPro:Malic_OxRdtase	InterPro:Malic_enzyme_CS	InterPro:NAD(P)-bd_dom_sf	PFAM:PF00390	PFAM:PF03949	PIRSF:PIRSF000106
PRINTS:PR00072	ScanProsite:PS00331	PANTHER:PTHR23406	PANTHER:PTHR23406:SF39	SMART:SM00919	SMART:SM01274
SUPFAM:SSF51735	SUPFAM:SSF53223	EnsemblPlantsGene:Solyc08g066360.2	EnsemblPlants:Solyc08g066360.2.1	UniParc:UPI000276BCA1	SEG:seg

Description

Malic enzyme [Source:UniProtKB/TrEMBL;Acc:K4CLL3]

Coordinates

chr8:-:54947399..54954256

Molecular Weight (calculated)

65301.7 Da

IEP (calculated)

5.837

GRAVY (calculated)

-0.123

Length

591 amino acids

Sequence
(BLAST)

001: MESTLKELTN GESVLDIKDK CGVAGGVEDI YGEDRATEDQ TITPWTFSVA SGYSLLRDPH YNKGLAFTEA ERDAHYLRGL LPPTVSSQQL QEKKVMHNLR
101: QYQVPLQRYM AMMDLQERNE RLFYKLLIDN VEELLPVVYT PTVGEACQKY GGIFRRPQGL FISLKEKGKI LEVLKNWPEK SIQVIVVTDG ERILGLGDLG
201: CQGMGIPVGK LSLYTALGGV RPSVCLPITI DVGTNNQKLL DDEFYIGLKQ KRATGQEYAE LLEEFMSAVK QNYGEKVLIQ FEDFANHNAF ELLAKYRTTH
301: LVFNDDIQGT ASVVLAGLIA SLKLLGGTLA DHTFLFLGAG EAGTGIAELI ALEITRKTSV PLEEARKKIW LVDSKGLIVS SRVESLQHFK KPWAHDHEPV
401: KELIDAVKAI KPTVLIGTSG VGKTFTKEVV EAMASLNPKP LIMALSNPTS QAECTAEEAY TWSKGHAIFA SGSPFDPVEF EGRTFVSGQA NNAYVFPGFG
501: LGIIMSGTIR VHDDMLLAAS EALASEVTED DYLKGRIYPP FTNIRKISAH IAAEVAAKAY ELGLATRLPR PSDLVKYAES CMYTPLYRSY R

Best Arabidopsis Sequence Match ( AT1G79750.1 )
(BLAST)

001: MISLTPSLFL NKTVVPGCST RLSLRQPRTI ITPPASLRVF SSLGSNQDPT GSVLIETTAT SSSSLETSAA DIVPKSTVSG GVQDVYGEDA ATEDMPITPW
101: SLSVASGYTL LRDPHHNKGL AFSHRERDAH YLRGLLPPTV ISQDLQVKKI MHTLRQYQVP LQKYMAMMDL QETNERLFYK LLIDHVEELL PVIYTPTVGE
201: ACQKYGSIFL RPQGLFISLK EKGKIHEVLR NWPEKNIQVI VVTDGERILG LGDLGCQGMG IPVGKLSLYT ALGGVRPSAC LPVTIDVGTN NEKLLNDEFY
301: IGLRQRRATG EEYSELMHEF MTAVKQNYGE KVVIQFEDFA NHNAFDLLAK YGTTHLVFND DIQGTASVVL AGLIAALRFV GGSLSDHRFL FLGAGEAGTG
401: IAELIALEIS KKSHIPLEEA RKNIWLVDSK GLIVSSRKES IQHFKKPWAH DHEPIRELVD AVKAIKPTVL IGTSGVGQTF TQDVVETMAK LNEKPIILSL
501: SNPTSQSECT AEEAYTWSQG RAIFASGSPF APVEYEGKTF VPGQANNAYI FPGFGLGLIM SGTIRVHDDM LLAASEALAE ELMEEHYEKG MIYPPFRNIR
601: KISARIAAKV AAKAYELGLA TRLPQPKELE QCAESSMYSP SYRSYR

Arabidopsis Description

NADP-ME4Malic enzyme [Source:UniProtKB/TrEMBL;Acc:A0A178W4H6]
SUBAcon: [plastid]

Hydropathy Plot

About CropPAL

The Protein Annotated Locations Database (CropPAL) houses large scale proteomic and GFP localization data from published experimental studies in Soybean (Glycine max), Maize (Zea mays), Wheat (Triticum aestivum), Barley (Hordeum vulgare), Rice (Oryza sativa), Field mustard (Brassica rapa), Canola (Brassica napus), Sorghum (Sorghum bicolor), Potato (Solanum tuberosum), Tomato (Solanum lycopersicum), Banana (Musa acuminata) and Wine grape (Vitis vinifera) as well as precomputed predictions for protein subcellular localizations using protein sequences.