Factsheet for Os05t0186300-01

Subcellular Localization

min:

: max

Winner_takes_all: plasma membrane, cytosol

Predictor Summary:

cytosol 4
plastid 2
mitochondrion 1

Predictors

GFP

MS/MS

Papers

plasma membrane: 27800704

msms PMID: 27800704 doi

J Cao, C Yang, L Li, L Jiang, Y Wu, C Wu, Q Bu, G Xia, X Liu, Y Luo, J Liu

Huai'an Institute of Agricultural Sciences, Huai'an, 223001, China., Institute of Analytical Chemistry and Synthetic and Functional Biomolecules Center, College of Chemistry and Molecular Engineering, Peking University, Beijing, 100871, China; and., Northeast Institute of Geography and Agroecology, Key Laboratory of Soybean Molecular Design Breeding, Chinese Academy of Sciences, Harbin, 150081, China., State Key Laboratory of Plant Genomics, Institute of Microbiology, Chinese Academy of Sciences, Beijing, 100101, China.

PPI

Inferred distinct locusB in Crop

locusB	locations
Os01t0218200-00
Os05t0210600-01
Os05t0587300-01

Inferred from Arabidopsis experimental PPI

Ath locusA	locusB	Ath locusB	Paper
AT5G11670.1	Os01t0218200-00	AT2G42580.1	19000166
AT5G11670.1	Os05t0210600-01	AT2G42580.1	19000166
AT5G11670.1	Os05t0587300-01	AT2G42580.1	19000166

Homology

Paralog

locus	Identity	Homology Identity

Ortholog

locus	Homology Species	Location	Identity	Homology Identity
TraesCS1B01G141700.1	Wheat	cytosol	90.7	90.7
TraesCS1A01G122500.1	Wheat	cytosol	90.53	90.53
Zm00001d037693_P003	Maize	extracellular	90.0	90.0
EES19164	Sorghum	cytosol, peroxisome, plastid	90.0	90.0
Zm00001d010358_P001	Maize	cytosol	88.42	88.42
TraesCS1D01G123400.2	Wheat	mitochondrion	86.67	86.21
VIT_04s0008g00180.t01	Wine grape	cytosol	84.21	82.05
HORVU1Hr1G028030.16	Barley	mitochondrion	88.25	81.92
CDX91199	Canola	cytosol	82.81	80.27
Bra009866.1-P	Field mustard	cytosol	82.63	80.1
Bra023336.1-P	Field mustard	cytosol	82.63	80.1
CDX88203	Canola	cytosol	82.63	80.1
CDY61446	Canola	cytosol	82.63	80.1
Solyc08g066360.2.1	Tomato	nucleus, plastid, unclear	82.98	80.03
CDX80544	Canola	cytosol	82.46	79.93
AT5G25880.1	Thale cress	cytosol	81.4	78.91
AT5G11670.1	Thale cress	cytosol	81.4	78.91
CDX78432	Canola	cytosol	80.88	78.4
CDX70409	Canola	cytosol	80.88	78.4
Bra006107.1-P	Field mustard	cytosol	80.88	78.4
KRH52781	Soybean	mitochondrion	84.39	77.71
KRH62099	Soybean	mitochondrion	84.74	77.65
Os01t0188400-01	Rice	plastid	85.09	75.9
Os01t0743500-01	Rice	plasma membrane, plastid	77.54	75.56
VIT_02s0012g02460.t01	Wine grape	endoplasmic reticulum, plasma membrane	54.74	72.22
Os01t0723400-04	Rice	plastid	80.18	71.07
Os02t0665000-01	Rice	cytosol	22.63	69.35
CDY02046	Canola	mitochondrion	65.09	66.37
Os07t0496200-01	Rice	mitochondrion	42.11	38.59
Os10t0503500-01	Rice	mitochondrion	41.23	37.9

Protein Annotations

Gene3D:3.40.50.10380	Gene3D:3.40.50.720	EntrezGene:4338007	MapMan:5.1.1.4	EMBL:AK070360	EMBL:AK099249
EMBL:AY435404	ProteinID:BAF16746.1	ProteinID:BAS92606.1	GO:GO:0000166	GO:GO:0003674	GO:GO:0003824
GO:GO:0004470	GO:GO:0004471	GO:GO:0004473	GO:GO:0005488	GO:GO:0005575	GO:GO:0005622
GO:GO:0005623	GO:GO:0005737	GO:GO:0006090	GO:GO:0006108	GO:GO:0008150	GO:GO:0008152
GO:GO:0009507	GO:GO:0009536	GO:GO:0009987	GO:GO:0016491	GO:GO:0046872	GO:GO:0051287
GO:GO:0055114	InterPro:IPR037062	InterPro:Malic_NAD-bd	InterPro:Malic_N_dom	InterPro:Malic_N_dom_sf	InterPro:Malic_OxRdtase
InterPro:Malic_enzyme_CS	InterPro:NAD(P)-bd_dom_sf	EnsemblPlantsGene:Os05g0186300	EnsemblPlants:Os05t0186300-01	PFAM:PF00390	PFAM:PF03949
PIRSF:PIRSF000106	PRINTS:PR00072	ScanProsite:PS00331	PANTHER:PTHR23406	PANTHER:PTHR23406:SF37	UniProt:Q6T5D1
SMART:SM00919	SMART:SM01274	SUPFAM:SSF51735	SUPFAM:SSF53223	UniParc:UPI00001D9476	RefSeq:XP_015640686.1
SEG:seg	:	:	:	:	:

Description

cytosolic NADP malic enzyme 2Similar to NADP-malic enzyme. (Os05t0186300-01);Non-protein coding transcript. (Os05t0186300-03)

Coordinates

chr5:-:5293558..5297417

Molecular Weight (calculated)

62938.4 Da

IEP (calculated)

5.525

GRAVY (calculated)

-0.131

Length

570 amino acids

Sequence
(BLAST)

001: MAGGGVEDTY GEDRATEDQL ITPWSFSVAS GYTLLRDPRH NKGLAFSEAE RDAHYLRGLL PPSIVSQELQ EKKLMHNLRN YTVPLQRYIA MMDLQERNER
101: LFYKLLIDNV EELLPVVYTP TVGEACQKYG SIYRRPQGLY ISLKDKGKIL EVLKNWPERS IQVIVVTDGE RILGLGDLGC QGMGIPVGKL SLYTALGGVR
201: PSACLPITID VGTNNESLLN DEFYIGLKQR RATGEEYHEL LEEFMTAVKQ NYGEKVLVQF EDFANHNAFD LLAKYSKSHL VFNDDIQGTA SVVLAGLIAA
301: LKVVGGTLAD HTYLFLGAGE AGTGIAELIA LEMSKQTEIP INDCRKKVWL VDSRGLIVES RKESLQHFKQ PFAHEHEPVK TLLEAVQSIK PTVLIGTSGV
401: GKTFTQEVVE AMAAFNEKPV IFALSNPTSH SECTAEEAYT WTKGSAVFAS GSPFDAVEYE GKTYVPGQSN NAYIFPGFGL GVVISGAIRV HDDMLLAASE
501: ALAEQVSEDN FARGLIFPPF TNIRKISAHI AAKVAAKAYE LGLASRLPRP DDLVKYAESC MYTPAYRCYR

Best Arabidopsis Sequence Match ( AT5G25880.1 )
(BLAST)

001: MGTNQTQISD EYVTGNSSGV GGGISDVYGE DSATLDQLVT PWVTSVASGY TLMRDPRYNK GLAFTDKERD AHYITGLLPP VVLSQDVQER KVMHNLRQYT
101: VPLQRYMALM DLQERNERLF YKLLIDNVEE LLPVVYTPTV GEACQKYGSI YRRPQGLYIS LKEKGKILEV LKNWPQRGIQ VIVVTDGERI LGLGDLGCQG
201: MGIPVGKLSL YTALGGIRPS ACLPITIDVG TNNEKLLNNE FYIGLKQKRA NGEEYAEFLQ EFMCAVKQNY GEKVLVQFED FANHHAFELL SKYCSSHLVF
301: NDDIQGTASV VLAGLIAAQK VLGKSLADHT FLFLGAGEAG TGIAELIALK ISKETGKPID ETRKKIWLVD SKGLIVSERK ESLQHFKQPW AHDHKPVKEL
401: LAAVNAIKPT VLIGTSGVGK TFTKEVVEAM ATLNEKPLIL ALSNPTSQAE CTAEEAYTWT KGRAIFASGS PFDPVQYDGK KFTPGQANNC YIFPGLGLGL
501: IMSGAIRVRD DMLLAASEAL ASQVTEENFA NGLIYPPFAN IRKISANIAA SVGAKTYELG LASNLPRPKD LVKMAESCMY SPVYRNFR

Arabidopsis Description

NADP-ME3Malic enzyme [Source:UniProtKB/TrEMBL;Acc:A0A178UAR6]
SUBAcon: [cytosol]

Hydropathy Plot

About CropPAL

The Protein Annotated Locations Database (CropPAL) houses large scale proteomic and GFP localization data from published experimental studies in Soybean (Glycine max), Maize (Zea mays), Wheat (Triticum aestivum), Barley (Hordeum vulgare), Rice (Oryza sativa), Field mustard (Brassica rapa), Canola (Brassica napus), Sorghum (Sorghum bicolor), Potato (Solanum tuberosum), Tomato (Solanum lycopersicum), Banana (Musa acuminata) and Wine grape (Vitis vinifera) as well as precomputed predictions for protein subcellular localizations using protein sequences.