CropPAL

Subcellular Localization

min:

: max

Winner_takes_all: cytosol

Predictor Summary:

cytosol 3
plastid 2
mitochondrion 1
peroxisome 1

Predictors

GFP

MS/MS

Papers

PPI

Inferred distinct locusB in Crop

locusB	locations
CDY42827
CDY51514
CDY55390
CDY61202

Inferred from Arabidopsis experimental PPI

Ath locusA	locusB	Ath locusB	Paper
AT5G11670.1	CDY42827	AT2G42580.1	19000166
AT5G11670.1	CDY51514	AT2G42580.1	19000166
AT5G11670.1	CDY55390	AT2G42580.1	19000166
AT5G11670.1	CDY61202	AT2G42580.1	19000166

Homology

Paralog

locus	Identity	Homology Identity

Ortholog

locus	Homology Species	Location	Identity	Homology Identity
Bra023336.1-P	Field mustard	cytosol	100.0	100.0
CDX91199	Canola	cytosol	99.66	99.66
CDX70409	Canola	cytosol	95.75	95.75
CDX78432	Canola	cytosol	95.75	95.75
AT5G11670.1	Thale cress	cytosol	94.73	94.73
CDX88203	Canola	cytosol	91.84	91.84
CDX80544	Canola	cytosol	91.5	91.5
Os05t0186300-01	Rice	cytosol, plasma membrane	80.1	82.63
VIT_04s0008g00180.t01	Wine grape	cytosol	80.95	81.37
Zm00001d037693_P003	Maize	extracellular	78.06	80.53
EES19164	Sorghum	cytosol, peroxisome, plastid	77.89	80.35
Solyc08g066360.2.1	Tomato	nucleus, plastid, unclear	79.59	79.19
TraesCS1A01G122500.1	Wheat	cytosol	76.7	79.12
Zm00001d010358_P001	Maize	cytosol	76.7	79.12
PGSC0003DMT400077452	Potato	cytosol	79.42	79.02
TraesCS1B01G141700.1	Wheat	cytosol	76.53	78.95
KRH52781	Soybean	mitochondrion	81.12	77.06
KRH62099	Soybean	mitochondrion	81.46	77.01
TraesCS3B01G128000.1	Wheat	cytosol	76.36	75.97
TraesCS1D01G123400.2	Wheat	mitochondrion	73.47	75.39
CDY35901	Canola	cytosol	73.47	74.23
CDY02046	Canola	mitochondrion	69.73	73.35
HORVU1Hr1G028030.16	Barley	mitochondrion	75.34	72.15
Zm00001d036873_P001	Maize	cytosol	6.12	72.0
VIT_02s0012g02460.t01	Wine grape	endoplasmic reticulum, plasma membrane	52.55	71.53
Zm00001d037961_P004	Maize	plastid	76.7	70.03
Os01t0188400-01	Rice	plastid	75.85	69.8
Zm00001d037962_P003	Maize	plastid	76.36	69.72
TraesCS3D01G110700.1	Wheat	plastid	76.02	69.63
TraesCS3A01G108900.1	Wheat	golgi	76.19	69.14
CDX82748	Canola	cytosol	68.54	69.13
CDX68115	Canola	plastid	76.53	68.91
CDX85926	Canola	plastid	73.47	68.9
KXG21798	Sorghum	plastid	76.36	68.86
EES00150	Sorghum	plastid	73.13	67.61
Zm00001d000316_P001	Maize	plastid	68.37	66.67
Os02t0665000-01	Rice	cytosol	20.24	63.98
Zm00001d034292_P001	Maize	cytosol	28.57	61.31
HORVU3Hr1G019790.9	Barley	plastid	75.17	60.63
OQU86165	Sorghum	mitochondrion	66.16	55.02
CDY33808	Canola	endoplasmic reticulum, extracellular	3.23	54.29
HORVU5Hr1G018620.1	Barley	cytosol	9.18	46.55
CDY37211	Canola	mitochondrion	40.82	38.84
CDX81448	Canola	mitochondrion	40.65	38.67
CDY68746	Canola	mitochondrion	38.61	37.52
CDX74340	Canola	mitochondrion	38.61	37.34
CDY07209	Canola	mitochondrion	38.61	37.27
CDY51151	Canola	mitochondrion	38.44	37.23
HORVU3Hr1G102340.13	Barley	plastid	11.05	27.08
Zm00001d003090_P001	Maize	mitochondrion	14.8	25.51
Zm00001d051182_P001	Maize	cytosol	6.63	17.65

Protein Annotations

Gene3D:3.40.50.10380	Gene3D:3.40.50.720	MapMan:5.1.1.4	GO:A0A078J560	UniProt:A0A078J560	EnsemblPlants:CDY61446
ProteinID:CDY61446	ProteinID:CDY61446.1	GO:GO:0000166	GO:GO:0003674	GO:GO:0003824	GO:GO:0004470
GO:GO:0004471	GO:GO:0005488	GO:GO:0008150	GO:GO:0008152	GO:GO:0016491	GO:GO:0046872
GO:GO:0051287	GO:GO:0055114	EnsemblPlantsGene:GSBRNA2T00033860001	InterPro:IPR037062	InterPro:Malic_NAD-bd	InterPro:Malic_N_dom
InterPro:Malic_N_dom_sf	InterPro:Malic_OxRdtase	InterPro:Malic_enzyme_CS	InterPro:NAD(P)-bd_dom_sf	PFAM:PF00390	PFAM:PF03949
PIRSF:PIRSF000106	PRINTS:PR00072	ScanProsite:PS00331	PANTHER:PTHR23406	PANTHER:PTHR23406:SF36	SMART:SM00919
SMART:SM01274	SUPFAM:SSF51735	SUPFAM:SSF53223	UniParc:UPI0002543398	:	:

Description

BnaA02g35010DMalic enzyme [Source:UniProtKB/TrEMBL;Acc:A0A078J560]

Coordinates

chrLK033996:-:30680..34575

Molecular Weight (calculated)

64483.5 Da

IEP (calculated)

5.722

GRAVY (calculated)

-0.099

Length

588 amino acids

Sequence
(BLAST)

001: MGSTPAEVPC DVVSDNRSGL GGGISDVYGE DLATLDQLVT PWVSSVASGY SLMRDPRYNK GLAFTDKERD AHYLTGLLPP VVLSQEVQER KLMHNLRQYT
101: VPLQRYMALM DLQERNERLF YKLLIDNVEE LLPVVYTPTV GEACQKYGSI FRKPQGLYIS LKEKGRILEV LKNWPQRGIQ VIVVTDGERI LGLGDLGCQG
201: MGIPVGKLSL YTALGGIRPS ACLPITIDVG TNNEKLLNDE FYIGLKQRRA TGQEYAEFLH EFMCAVKQNY GEKVLVQFED FANHNAFDLL SKYSTSHLVF
301: NDDIQGTASV VLAGLIAAQK VLGKSLADHT FLFLGAGEAG TGIAELIALK ISKETGAPID ETRKKIWLVD SKGLIVSSRK ESLQHFKQPW AHEHEPVKNL
401: IGAVNAIKPT VLIGTSGVGQ TFTQEVVEAM ATNNETPLIL ALSNPTSQAE CTAEQAYTWT KGRAIFGSGS PFDPVEYDGK TYFPGQANNC YIFPGLGLGL
501: IMSGAIRVRD DMLLAASEAL AAQVTEENFA NGLIYPPFSN IREISANIAA SVAAKTYELG LASNLPRPKD LVKFAESCMY SPVYRNYR

Best Arabidopsis Sequence Match ( AT5G11670.1 )
(BLAST)

001: MGSTPTDLPG EDVADNRSGV GGGISDVYGE DSATLDQLVT PWVTSVASGY TLMRDPRYNK GLAFTDKERD AHYLTGLLPP VILSQDVQER KVMHNLRQYT
101: VPLQRYMALM DLQERNERLF YKLLIDNVEE LLPVVYTPTV GEACQKYGSI FRKPQGLYIS LNEKGKILEV LKNWPQRGIQ VIVVTDGERI LGLGDLGCQG
201: MGIPVGKLSL YTALGGIRPS ACLPITIDVG TNNEKLLNDE FYIGLKQRRA TGQEYAEFLH EFMCAVKQNY GEKVLVQFED FANHNAFDLL SKYSDSHLVF
301: NDDIQGTASV VLAGLIAAQK VLGKKLADHT FLFLGAGEAG TGIAELIALK ISKETGAPIT ETRKKIWLVD SKGLIVSSRK ESLQHFKQPW AHEHKPVKDL
401: IGAVNAIKPT VLIGTSGVGQ TFTKEVVEAM ATNNEKPLIL ALSNPTSQAE CTAEQAYTWT KGRAIFGSGS PFDPVVYDGK TYLPGQANNC YIFPGLGLGL
501: IMSGAIRVRD DMLLAASEAL AAQVTEEHYA NGLIYPPFSN IREISANIAA CVAAKTYDLG LASNLPRAKD LVKFAESSMY SPVYRNYR

Arabidopsis Description

NADP-ME2NADP-dependent malic enzyme 2 [Source:UniProtKB/Swiss-Prot;Acc:Q9LYG3]
SUBAcon: [cytosol]

Hydropathy Plot

About CropPAL

The Protein Annotated Locations Database (CropPAL) houses large scale proteomic and GFP localization data from published experimental studies in Soybean (Glycine max), Maize (Zea mays), Wheat (Triticum aestivum), Barley (Hordeum vulgare), Rice (Oryza sativa), Field mustard (Brassica rapa), Canola (Brassica napus), Sorghum (Sorghum bicolor), Potato (Solanum tuberosum), Tomato (Solanum lycopersicum), Banana (Musa acuminata) and Wine grape (Vitis vinifera) as well as precomputed predictions for protein subcellular localizations using protein sequences.