CropPAL

Subcellular Localization

min:

: max

Winner_takes_all: mitochondrion

Predictor Summary:

plastid 1
mitochondrion 7

Predictors

GFP

MS/MS

Papers

PPI

Inferred distinct locusB in Crop

locusB	locations
CDY30300
CDY52581
CDY52582

Inferred from Arabidopsis experimental PPI

Ath locusA	locusB	Ath locusB	Paper
AT4G00570.1	CDY30300	AT2G18876.1	21798944
AT4G00570.1	CDY52581	AT2G18876.1	21798944
AT4G00570.1	CDY52582	AT2G18876.1	21798944

Homology

Paralog

locus	Identity	Homology Identity

Ortholog

locus	Homology Species	Location	Identity	Homology Identity
CDX74340	Canola	mitochondrion	99.67	99.18
CDY07209	Canola	mitochondrion	97.85	97.21
CDY51151	Canola	mitochondrion	97.52	97.2
Bra000967.1-P	Field mustard	mitochondrion	98.84	96.45
AT4G00570.1	Thale cress	mitochondrion	92.07	91.76
VIT_15s0046g03670.t01	Wine grape	mitochondrion	84.13	84.13
CDY33808	Canola	endoplasmic reticulum, extracellular	4.79	82.86
KRH48207	Soybean	mitochondrion	82.48	82.62
KRH65116	Soybean	mitochondrion	81.65	81.79
KRH00689	Soybean	mitochondrion	80.17	80.83
Solyc01g094200.2.1	Tomato	plastid	79.83	80.37
KRH40505	Soybean	mitochondrion	79.83	80.37
PGSC0003DMT400000066	Potato	mitochondrion	79.83	77.03
GSMUA_Achr1P00210_001	Banana	mitochondrion	75.7	75.83
KXG38219	Sorghum	mitochondrion	76.2	74.6
Os10t0503500-01	Rice	mitochondrion	75.87	74.03
TraesCS1D01G164800.4	Wheat	mitochondrion, unclear	75.37	73.91
TraesCS1B01G190400.5	Wheat	mitochondrion	73.22	72.15
Zm00001d013911_P001	Maize	mitochondrion	72.4	68.44
HORVU1Hr1G045720.1	Barley	plastid	75.7	63.0
CDY37211	Canola	mitochondrion	62.98	61.65
CDX81448	Canola	mitochondrion	62.98	61.65
CDY35901	Canola	cytosol	37.85	39.35
CDX78432	Canola	cytosol	38.02	39.12
CDX70409	Canola	cytosol	38.02	39.12
CDX88203	Canola	cytosol	37.85	38.95
CDX80544	Canola	cytosol	37.85	38.95
CDY61446	Canola	cytosol	37.52	38.61
CDX91199	Canola	cytosol	37.52	38.61
CDX82748	Canola	cytosol	35.54	36.88
CDX68115	Canola	plastid	37.36	34.61
CDX85926	Canola	plastid	35.04	33.81
CDY02046	Canola	mitochondrion	30.41	32.92

Protein Annotations

MapMan:2.3.9	Gene3D:3.40.50.10380	Gene3D:3.40.50.720	GO:A0A078JT02	UniProt:A0A078JT02	EnsemblPlants:CDY68746
ProteinID:CDY68746	ProteinID:CDY68746.1	GO:GO:0000166	GO:GO:0003674	GO:GO:0003824	GO:GO:0004470
GO:GO:0004471	GO:GO:0005488	GO:GO:0008150	GO:GO:0008152	GO:GO:0016491	GO:GO:0046872
GO:GO:0051287	GO:GO:0055114	EnsemblPlantsGene:GSBRNA2T00077578001	InterPro:IPR037062	InterPro:Malic_NAD-bd	InterPro:Malic_N_dom
InterPro:Malic_N_dom_sf	InterPro:Malic_OxRdtase	InterPro:Malic_enzyme_CS	InterPro:NAD(P)-bd_dom_sf	PFAM:PF00390	PFAM:PF03949
PIRSF:PIRSF000106	PRINTS:PR00072	ScanProsite:PS00331	PANTHER:PTHR23406	PANTHER:PTHR23406:SF32	SMART:SM00919
SMART:SM01274	SUPFAM:SSF51735	SUPFAM:SSF53223	UniParc:UPI0004EC4E0C	SEG:seg	:

Description

BnaCnng60330DMalic enzyme [Source:UniProtKB/TrEMBL;Acc:A0A078JT02]

Coordinates

chrLK037883:-:570..4518

Molecular Weight (calculated)

66796.7 Da

IEP (calculated)

7.088

GRAVY (calculated)

-0.094

Length

605 amino acids

Sequence
(BLAST)

001: MMWRNIARFS KAAGRTGGSR RCLSTAIPGP CIVHKRGSDI LHDPWFNKDT GFPLTERDRL GLRGLLPPRI ISFEQQYDRF IESFRSLERN TLGQPDNVVS
101: LAKWRILNRL HDRNETLYYR VLIDNIKDFA PIIYTPTVGL VCQNYSGLYR RPRGMYFSAK DKGEMMSMIY NWPAHQVDMI VITDGSRILG LGDLGVQGIG
201: IPIGKLDMYV AAAGINPQRV LPIMLDVGTN NQKLLQNPLY LGLRQPRLEG DEYLEIIDEF MEAAFTRWPK AVVQFEDFQA KWAFETLDRY RKKFCMFNDD
301: VQGTAGVALA GLLGTVRAQG RPLSDFVNQK IVVVGAGSAG LGVTKTAVQA VARMAGISFA EATKNFYLID KDGLVTTERS KLDPAVVPFA KNPAEIREGA
401: SIVEVVKTVR PHVLLGLSGV GGIFNEEVLK AMRESDSCKP AIFAMSNPTL NAECTAADAF KHAGENIVFG SGSPFENVQL ENGNVGHVNQ ANNMYLFPGI
501: GLGTLLSGAR IVTDGMLLAA AECLASYMTD EEVQKGILYP SINNIRHITA EVGAAVLRAA VSDDIVEGHG DVGPRDLSHM SKEETVDYIT RNMWFPIYSP
601: LVHEK

Best Arabidopsis Sequence Match ( AT4G00570.1 )
(BLAST)

001: MMWKNIAGLS KAAAAARTHG SRRCFSTAIP GPCIVHKRGA DILHDPWFNK DTGFPLTERD RLGIRGLLPP RVMTCVQQCD RFIESFRSLE NNTKGEPENV
101: VALAKWRMLN RLHDRNETLY YRVLIDNIKD FAPIIYTPTV GLVCQNYSGL YRRPRGMYFS AKDKGEMMSM IYNWPAPQVD MIVITDGSRI LGLGDLGVQG
201: IGIPIGKLDM YVAAAGINPQ RVLPIMLDVG TNNEKLLQND LYLGVRQPRL EGEEYLEIID EFMEAAFTRW PKAVVQFEDF QAKWAFGTLE RYRKKFCMFN
301: DDVQGTAGVA LAGLLGTVRA QGRPISDFVN QKIVVVGAGS AGLGVTKMAV QAVARMAGIS ESEATKNFYL IDKDGLVTTE RTKLDPGAVL FAKNPAEIRE
401: GASIVEVVKK VRPHVLLGLS GVGGIFNEEV LKAMRESDSC KPAIFAMSNP TLNAECTAAD AFKHAGGNIV FASGSPFENV ELENGKVGHV NQANNMYLFP
501: GIGLGTLLSG ARIVTDGMLQ AASECLASYM TDEEVQKGIL YPSINNIRHI TAEVGAAVLR AAVTDDIAEG HGDVGPKDLS HMSKEDTVNY ITRNMWFPVY
601: SPLVHEK

Arabidopsis Description

NAD-ME2NAD-dependent malic enzyme 2, mitochondrial [Source:UniProtKB/Swiss-Prot;Acc:Q8L7K9]
SUBAcon: [mitochondrion]

Hydropathy Plot

About CropPAL

The Protein Annotated Locations Database (CropPAL) houses large scale proteomic and GFP localization data from published experimental studies in Soybean (Glycine max), Maize (Zea mays), Wheat (Triticum aestivum), Barley (Hordeum vulgare), Rice (Oryza sativa), Field mustard (Brassica rapa), Canola (Brassica napus), Sorghum (Sorghum bicolor), Potato (Solanum tuberosum), Tomato (Solanum lycopersicum), Banana (Musa acuminata) and Wine grape (Vitis vinifera) as well as precomputed predictions for protein subcellular localizations using protein sequences.