CropPAL

Subcellular Localization

min:

: max

Winner_takes_all: mitochondrion

Predictor Summary:

plastid 1
mitochondrion 9
peroxisome 1

Predictors

GFP

MS/MS

Papers

nucleus: 21132161
mitochondrion: 27780359

msms PMID: 21132161 doi

B Cooper, KB Campbell, J Feng, WM Garrett, R Frederick

Soybean Genomics and Improvement Laboratory, USDA-ARS, Beltsville, MD 20705, USA. bret.cooper@ars.usda.gov

msms PMID: 27780359 doi

G Mustafa, S Komatsu

Graduate School of Life and Environmental Science, University of Tsukuba , Tsukuba 305-8572, Japan., National Institute of Crop Science, National Agriculture and Food Research Organization , Tsukuba 305-8518, Japan.

PPI

Inferred distinct locusB in Crop

locusB	locations
KRH02865
KRH10294
KRH13062
KRH19028
KRH23029
KRH24840
KRH29844
KRH38497

Inferred from Arabidopsis experimental PPI

Ath locusA	locusB	Ath locusB	Paper
AT4G00570.1	KRH02865	AT2G18876.1	21798944
AT4G00570.1	KRH13062	AT2G18876.1	21798944
AT4G00570.1	KRH19028	AT2G18876.1	21798944
AT4G00570.1	KRH38497	AT2G18876.1	21798944
AT4G00570.1	KRH10294	AT3G46090.1	17259181
AT4G00570.1	KRH23029	AT3G46090.1	17259181
AT4G00570.1	KRH24840	AT3G46090.1	17259181
AT4G00570.1	KRH29844	AT3G46090.1	17259181

Homology

Paralog

locus	Identity	Homology Identity

Ortholog

locus	Homology Species	Location	Identity	Homology Identity
KRH65116	Soybean	mitochondrion	98.18	98.18
KRH00689	Soybean	mitochondrion	86.75	87.33
KRH40505	Soybean	mitochondrion	85.93	86.36
VIT_15s0046g03670.t01	Wine grape	mitochondrion	85.6	85.45
Bra037347.1-P	Field mustard	mitochondrion	80.63	82.96
CDY68746	Canola	mitochondrion	82.62	82.48
CDY51151	Canola	mitochondrion	82.62	82.21
Solyc01g094200.2.1	Tomato	plastid	81.79	82.2
CDX74340	Canola	mitochondrion	82.62	82.07
CDY07209	Canola	mitochondrion	82.45	81.77
AT4G00570.1	Thale cress	mitochondrion	81.62	81.22
Bra000967.1-P	Field mustard	mitochondrion	82.28	80.16
GSMUA_Achr1P00210_001	Banana	mitochondrion	79.14	79.14
PGSC0003DMT400000066	Potato	mitochondrion	81.95	78.95
KXG38219	Sorghum	mitochondrion	76.66	74.92
TraesCS1D01G164800.4	Wheat	mitochondrion, unclear	75.83	74.23
Os10t0503500-01	Rice	mitochondrion	75.66	73.71
TraesCS1B01G190400.5	Wheat	mitochondrion	73.68	72.48
Zm00001d013911_P001	Maize	mitochondrion	72.68	68.59
KRH66385	Soybean	mitochondrion	66.39	64.47
HORVU1Hr1G045720.1	Barley	plastid	76.16	63.27
KRH74263	Soybean	cytosol	38.24	39.09
KRH07269	Soybean	cytosol	38.24	39.09
KRH60218	Soybean	mitochondrion	39.57	37.76
KRH52781	Soybean	mitochondrion	38.08	37.16
KRH62099	Soybean	mitochondrion	38.24	37.14
KRH41507	Soybean	cytosol, peroxisome, plastid	34.11	36.72
KRH09931	Soybean	mitochondrion, plastid	38.24	35.93
KRH44278	Soybean	plastid	37.91	35.73
KRH23402	Soybean	nucleus	38.24	35.7
KRH47214	Soybean	mitochondrion	28.31	29.74
KRH13473	Soybean	plastid	5.96	29.27
KRH12915	Soybean	cytosol	4.64	25.23
KRH01213	Soybean	cytosol, plastid	8.44	17.96

Protein Annotations

EntrezGene:100806545	MapMan:2.3.9	Gene3D:3.40.50.10380	Gene3D:3.40.50.720	EMBL:ACUP02004210	EnsemblPlantsGene:GLYMA_07G074700
GO:GO:0000166	GO:GO:0003674	GO:GO:0003824	GO:GO:0004470	GO:GO:0004471	GO:GO:0005488
GO:GO:0005575	GO:GO:0005622	GO:GO:0005623	GO:GO:0005737	GO:GO:0005739	GO:GO:0006090
GO:GO:0006108	GO:GO:0008150	GO:GO:0008152	GO:GO:0009507	GO:GO:0009536	GO:GO:0009987
GO:GO:0016491	GO:GO:0046872	GO:GO:0051287	GO:GO:0055114	UniProt:I1KIF0	InterPro:IPR037062
EnsemblPlants:KRH48207	ProteinID:KRH48207	ProteinID:KRH48207.1	InterPro:Malic_NAD-bd	InterPro:Malic_N_dom	InterPro:Malic_N_dom_sf
InterPro:Malic_OxRdtase	InterPro:Malic_enzyme_CS	InterPro:NAD(P)-bd_dom_sf	PFAM:PF00390	PFAM:PF03949	PIRSF:PIRSF000106
PRINTS:PR00072	ScanProsite:PS00331	PANTHER:PTHR23406	PANTHER:PTHR23406:SF32	SMART:SM00919	SMART:SM01274
SUPFAM:SSF51735	SUPFAM:SSF53223	UniParc:UPI0001CAC0C5	SEG:seg	:	:

Description

hypothetical protein

Coordinates

chr7:-:6793757..6801619

Molecular Weight (calculated)

66651.7 Da

IEP (calculated)

6.452

GRAVY (calculated)

-0.072

Length

604 amino acids

Sequence
(BLAST)

001: MWKLVRYAAA SNLARSRRFS AAIPGPCIVH KRGADILHDP WFNKDTGFPL TERDRLGLRG LLPPRVISFE QQYDRFMNSF RSLENNTQGQ PDKVVSLAKW
101: RILNRLHDRN ETLYYRVLID NIKEFAPIIY TPTVGLVCQN YSGLFRRPRG MYFSAKDKGE MMSMIYNWPA HEVDMIVLTD GSRILGLGDL GVQGIGIPIG
201: KLDVYVAAAG INPQRILPVM LDVGTNNQKL LEDRLYLGLR QPRLEGEEYL SIIDEFMEAV HARWPKAIVQ FEDFQMKWAF ETLKRYQKKF CMFNDDIQGT
301: AGVALAGLLG TVRAQGRPLT DFVNQKIVVV GAGSAGLGVL KMAIQAVAKI SGCSELAAKS QFYLIDKDGL VTTERNNLDP AAAPFAKNPR DIEGLTEGAS
401: IIEVVKKIRP HVLLGLSGVG GIFNEEVLKA MRESVSTKPA IFAMSNPTMN AECTAIDAFK HAGENMVFAS GSPFENVDLG NGIVGHVNQA NNMYLFPGIG
501: LGTLLSGAHL ITDGMLQAAA ECLASYMAEE DILKGILYPS VDSIRDVTAE VGAAVLRAAV EEELAEGNGD VGPKELSHMS KDETVEYVRS NMWYPVYSPL
601: VHEK

Best Arabidopsis Sequence Match ( AT4G00570.1 )
(BLAST)

001: MMWKNIAGLS KAAAAARTHG SRRCFSTAIP GPCIVHKRGA DILHDPWFNK DTGFPLTERD RLGIRGLLPP RVMTCVQQCD RFIESFRSLE NNTKGEPENV
101: VALAKWRMLN RLHDRNETLY YRVLIDNIKD FAPIIYTPTV GLVCQNYSGL YRRPRGMYFS AKDKGEMMSM IYNWPAPQVD MIVITDGSRI LGLGDLGVQG
201: IGIPIGKLDM YVAAAGINPQ RVLPIMLDVG TNNEKLLQND LYLGVRQPRL EGEEYLEIID EFMEAAFTRW PKAVVQFEDF QAKWAFGTLE RYRKKFCMFN
301: DDVQGTAGVA LAGLLGTVRA QGRPISDFVN QKIVVVGAGS AGLGVTKMAV QAVARMAGIS ESEATKNFYL IDKDGLVTTE RTKLDPGAVL FAKNPAEIRE
401: GASIVEVVKK VRPHVLLGLS GVGGIFNEEV LKAMRESDSC KPAIFAMSNP TLNAECTAAD AFKHAGGNIV FASGSPFENV ELENGKVGHV NQANNMYLFP
501: GIGLGTLLSG ARIVTDGMLQ AASECLASYM TDEEVQKGIL YPSINNIRHI TAEVGAAVLR AAVTDDIAEG HGDVGPKDLS HMSKEDTVNY ITRNMWFPVY
601: SPLVHEK

Arabidopsis Description

NAD-ME2NAD-dependent malic enzyme 2, mitochondrial [Source:UniProtKB/Swiss-Prot;Acc:Q8L7K9]
SUBAcon: [mitochondrion]

Hydropathy Plot

About CropPAL

The Protein Annotated Locations Database (CropPAL) houses large scale proteomic and GFP localization data from published experimental studies in Soybean (Glycine max), Maize (Zea mays), Wheat (Triticum aestivum), Barley (Hordeum vulgare), Rice (Oryza sativa), Field mustard (Brassica rapa), Canola (Brassica napus), Sorghum (Sorghum bicolor), Potato (Solanum tuberosum), Tomato (Solanum lycopersicum), Banana (Musa acuminata) and Wine grape (Vitis vinifera) as well as precomputed predictions for protein subcellular localizations using protein sequences.