CropPAL

Subcellular Localization

min:

: max

Winner_takes_all: nucleus

Predictor Summary:

plastid 8
mitochondrion 3

Predictors

GFP

MS/MS

Papers

nucleus: 21132161

msms PMID: 21132161 doi

B Cooper, KB Campbell, J Feng, WM Garrett, R Frederick

Soybean Genomics and Improvement Laboratory, USDA-ARS, Beltsville, MD 20705, USA. bret.cooper@ars.usda.gov

PPI

Inferred distinct locusB in Crop

locusB	locations
KRG99623
KRH07269
KRH07429
KRH12915
KRH41507
KRH46625
KRH60218
KRH66142
KRH74263

Inferred from Arabidopsis experimental PPI

Ath locusA	locusB	Ath locusB	Paper
AT1G79750.1	KRH07269	AT2G19900.1	21798944
AT1G79750.1	KRH12915	AT2G19900.1	21798944
AT1G79750.1	KRH41507	AT2G19900.1	21798944
AT1G79750.1	KRH60218	AT2G19900.1	21798944
AT1G79750.1	KRH74263	AT2G19900.1	21798944
AT1G79750.1	KRG99623	AT3G07310.1	21798944
AT1G79750.1	KRH07429	AT3G07310.1	21798944
AT1G79750.1	KRH46625	AT3G07310.1	21798944
AT1G79750.1	KRH66142	AT3G07310.1	21798944

Homology

Paralog

locus	Identity	Homology Identity

Ortholog

locus	Homology Species	Location	Identity	Homology Identity
KRH09931	Soybean	mitochondrion, plastid	97.53	98.13
KRH44278	Soybean	plastid	82.38	83.15
KRH07269	Soybean	cytosol	72.95	79.86
KRH74263	Soybean	cytosol	72.64	79.53
KRH12915	Soybean	cytosol	13.45	78.38
KRH52781	Soybean	mitochondrion	74.65	78.03
KRH62099	Soybean	mitochondrion	74.96	77.97
VIT_09s0002g03620.t01	Wine grape	cytosol	77.13	77.97
AT1G79750.1	Thale cress	plastid	76.82	76.94
KRH60218	Soybean	mitochondrion	74.65	76.3
PGSC0003DMT400006726	Potato	plastid	75.42	76.01
PGSC0003DMT400068027	Potato	plastid	74.65	75.47
CDX85926	Canola	plastid	73.11	75.44
CDX68115	Canola	plastid	75.42	74.73
Bra003738.1-P	Field mustard	plastid	72.49	73.86
KRH41507	Soybean	cytosol, peroxisome, plastid	62.75	72.37
Solyc08g066290.2.1	Tomato	cytosol	8.04	72.22
KRH13473	Soybean	plastid	12.67	66.67
KRH47214	Soybean	mitochondrion	57.03	64.17
KRH01213	Soybean	cytosol, plastid	19.17	43.66
KRH65116	Soybean	mitochondrion	35.86	38.41
KRH48207	Soybean	mitochondrion	35.7	38.24
KRH00689	Soybean	mitochondrion	35.24	38.0
KRH40505	Soybean	mitochondrion	35.24	37.94
KRH66385	Soybean	mitochondrion	36.01	37.46

Protein Annotations

EntrezGene:100800844	Gene3D:3.40.50.10380	Gene3D:3.40.50.720	MapMan:5.1.1.4	EMBL:ACUP02008465	EnsemblPlantsGene:GLYMA_13G354900
GO:GO:0000166	GO:GO:0003674	GO:GO:0003824	GO:GO:0004470	GO:GO:0004471	GO:GO:0004473
GO:GO:0005488	GO:GO:0005575	GO:GO:0005622	GO:GO:0005623	GO:GO:0005737	GO:GO:0006090
GO:GO:0006108	GO:GO:0008150	GO:GO:0008152	GO:GO:0009507	GO:GO:0009536	GO:GO:0009987
GO:GO:0016491	GO:GO:0046872	GO:GO:0051287	GO:GO:0055114	UniProt:I1M5G8	InterPro:IPR037062
EnsemblPlants:KRH23402	ProteinID:KRH23402	ProteinID:KRH23402.1	InterPro:Malic_NAD-bd	InterPro:Malic_N_dom	InterPro:Malic_N_dom_sf
InterPro:Malic_OxRdtase	InterPro:Malic_enzyme_CS	InterPro:NAD(P)-bd_dom_sf	PFAM:PF00390	PFAM:PF03949	PIRSF:PIRSF000106
PRINTS:PR00072	ScanProsite:PS00331	PFscan:PS51257	PANTHER:PTHR23406	PANTHER:PTHR23406:SF36	SMART:SM00919
SMART:SM01274	SUPFAM:SSF51735	SUPFAM:SSF53223	UniParc:UPI000233B3C2	SEG:seg	:

Description

hypothetical protein

Coordinates

chr13:-:44343888..44349533

Molecular Weight (calculated)

71313.6 Da

IEP (calculated)

7.609

GRAVY (calculated)

-0.211

Length

647 amino acids

Sequence
(BLAST)

001: MFSSTRCAFL SNSGLGGCSS LCDAAQKKRS TRLRVVSMTP SSSSSSRSGD RNGSVVMETP LKELKKESTV ADVDDNAISA GGPQDVYGED RATEDHFVTP
101: WSVSVASGYT LLRDPHFNKG LAFTEDERDA HYLRGLLPPS VIPQETQVKK MIQHVRQYQV PLQKYMAMMD LQERNERLFY KLLIDHVEEL LPVVYTPTVG
201: EACQKYGSIF MHPQGLYISL KEKGKIREVL RNWPEKNIQV IVVTDGERIL GLGDLGCQGM GIPVGKLSLY TALGGVRPSA CLPITIDVGT NNEKLLNDEL
301: YIGLKQRRAT GQEYAELMHE FMTAVKQTYG EKVLIQFEDF ANHNAFNLLE KYRSTHLVFN DDIQGTASVV LAGLVASLKL VGGNLADHRF LFLGAGEAGT
401: GIAELIALET SKQTNAPLEE VRKNIWLVDS KGLIVSSRKD SLQHFKKPWA HEHEPVKNLL DAVNKIKPTV LIGTSGQGRT FTKEVIEAMA SINKRPIILS
501: LSNPTSQSEC TAEEAYKWSQ GRAIFASGSP FPPVEYEGKV FVPGQANNAY IFPGFGLGLI MSGTIRVHDD LLLAASEALA AQVSQENFDK GLIYPPFTNI
601: RKISAHIAAN VAAKAYELGL ATRLPQPKDL VKFAESCMYT PAYRSYR

Best Arabidopsis Sequence Match ( AT1G79750.1 )
(BLAST)

001: MISLTPSLFL NKTVVPGCST RLSLRQPRTI ITPPASLRVF SSLGSNQDPT GSVLIETTAT SSSSLETSAA DIVPKSTVSG GVQDVYGEDA ATEDMPITPW
101: SLSVASGYTL LRDPHHNKGL AFSHRERDAH YLRGLLPPTV ISQDLQVKKI MHTLRQYQVP LQKYMAMMDL QETNERLFYK LLIDHVEELL PVIYTPTVGE
201: ACQKYGSIFL RPQGLFISLK EKGKIHEVLR NWPEKNIQVI VVTDGERILG LGDLGCQGMG IPVGKLSLYT ALGGVRPSAC LPVTIDVGTN NEKLLNDEFY
301: IGLRQRRATG EEYSELMHEF MTAVKQNYGE KVVIQFEDFA NHNAFDLLAK YGTTHLVFND DIQGTASVVL AGLIAALRFV GGSLSDHRFL FLGAGEAGTG
401: IAELIALEIS KKSHIPLEEA RKNIWLVDSK GLIVSSRKES IQHFKKPWAH DHEPIRELVD AVKAIKPTVL IGTSGVGQTF TQDVVETMAK LNEKPIILSL
501: SNPTSQSECT AEEAYTWSQG RAIFASGSPF APVEYEGKTF VPGQANNAYI FPGFGLGLIM SGTIRVHDDM LLAASEALAE ELMEEHYEKG MIYPPFRNIR
601: KISARIAAKV AAKAYELGLA TRLPQPKELE QCAESSMYSP SYRSYR

Arabidopsis Description

NADP-ME4Malic enzyme [Source:UniProtKB/TrEMBL;Acc:A0A178W4H6]
SUBAcon: [plastid]

Hydropathy Plot

About CropPAL

The Protein Annotated Locations Database (CropPAL) houses large scale proteomic and GFP localization data from published experimental studies in Soybean (Glycine max), Maize (Zea mays), Wheat (Triticum aestivum), Barley (Hordeum vulgare), Rice (Oryza sativa), Field mustard (Brassica rapa), Canola (Brassica napus), Sorghum (Sorghum bicolor), Potato (Solanum tuberosum), Tomato (Solanum lycopersicum), Banana (Musa acuminata) and Wine grape (Vitis vinifera) as well as precomputed predictions for protein subcellular localizations using protein sequences.