Factsheet for VIT_09s0002g03620.t01

Subcellular Localization

min:

: max

Winner_takes_all: cytosol

Predictor Summary:

plastid 10
cytosol 1

Predictors

GFP

MS/MS

Papers

cytosol: 21379583

msms PMID: 21379583 doi

RG Sharathchandra, C Stander, D Jacobson, B Ndimba, MA Vivier

Department of Viticulture and Oenology, Institute for Wine Biotechnology, Stellenbosch University, Stellenbosch, South Africa.

PPI

Inferred distinct locusB in Crop

locusB	locations
VIT_00s0360g00020.t01
VIT_00s2037g00010.t01
VIT_11s0016g03210.t01

Inferred from Arabidopsis experimental PPI

Ath locusA	locusB	Ath locusB	Paper
AT1G79750.1	VIT_11s0016g03210.t01	AT2G19900.1	21798944
AT1G79750.1	VIT_00s0360g00020.t01	AT3G07310.1	21798944
AT1G79750.1	VIT_00s2037g00010.t01	AT3G07310.1	21798944

Homology

Paralog

locus	Identity	Homology Identity

Ortholog

locus	Homology Species	Location	Identity	Homology Identity
VIT_04s0008g00180.t01	Wine grape	cytosol	77.97	85.3
VIT_11s0016g03210.t01	Wine grape	cytosol	78.44	84.94
PGSC0003DMT400006726	Potato	plastid	80.47	80.22
PGSC0003DMT400068027	Potato	plastid	80.16	80.16
Solyc12g044600.2.1	Tomato	plastid	79.84	79.84
Solyc03g120990.2.1	Tomato	plastid	79.69	79.44
KRH09931	Soybean	mitochondrion, plastid	78.75	78.38
KRH23402	Soybean	nucleus	77.97	77.13
HORVU5Hr1G053200.1	Barley	endoplasmic reticulum, extracellular	7.81	76.92
KRH44278	Soybean	plastid	76.56	76.44
AT1G79750.1	Thale cress	plastid	76.41	75.7
CDX85926	Canola	plastid	72.5	74.0
CDX68115	Canola	plastid	75.16	73.66
VIT_02s0012g02460.t01	Wine grape	endoplasmic reticulum, plasma membrane	49.69	73.61
Bra003738.1-P	Field mustard	plastid	72.19	72.76
VIT_16s0039g00580.t01	Wine grape	cytosol	5.47	68.63
VIT_00s0342g00030.t01	Wine grape	cytosol	5.94	61.29
KRH47214	Soybean	mitochondrion	54.22	60.35
VIT_16s0039g01050.t01	Wine grape	cytosol	5.94	60.32
KRH13473	Soybean	plastid	11.56	60.16
VIT_00s0183g00140.t01	Wine grape	cytosol	7.03	57.69
VIT_00s0279g00080.t01	Wine grape	cytosol	12.34	55.24
VIT_16s0115g00440.t01	Wine grape	cytosol	12.19	54.55
VIT_03s0038g00040.t01	Wine grape	extracellular	6.09	54.17
VIT_16s0039g00360.t01	Wine grape	extracellular	5.94	52.78
VIT_15s0045g00190.t01	Wine grape	extracellular	5.78	51.39
VIT_15s0021g00500.t01	Wine grape	mitochondrion	38.44	39.36
VIT_15s0046g03670.t01	Wine grape	mitochondrion	37.19	39.34
KRH01213	Soybean	cytosol, plastid	16.56	37.32

Protein Annotations

EntrezGene:100233075	wikigene:100233075	Gene3D:3.40.50.10380	Gene3D:3.40.50.720	MapMan:5.1.1.4	ProteinID:AAB08874
ProteinID:AAB08874.1	ProteinID:CBI36068	ProteinID:CBI36068.3	UniProt:D7U0C2	EMBL:FN596494	GO:GO:0000166
GO:GO:0003674	GO:GO:0003824	GO:GO:0004470	GO:GO:0004471	GO:GO:0004473	GO:GO:0005488
GO:GO:0005575	GO:GO:0005622	GO:GO:0005623	GO:GO:0005737	GO:GO:0006090	GO:GO:0006108
GO:GO:0008150	GO:GO:0008152	GO:GO:0009507	GO:GO:0009536	GO:GO:0009987	GO:GO:0016491
GO:GO:0046872	GO:GO:0051287	GO:GO:0055114	InterPro:IPR037062	InterPro:Malic_NAD-bd	InterPro:Malic_N_dom
InterPro:Malic_N_dom_sf	InterPro:Malic_OxRdtase	InterPro:Malic_enzyme_CS	InterPro:NAD(P)-bd_dom_sf	PFAM:PF00390	PFAM:PF03949
PIRSF:PIRSF000106	PRINTS:PR00072	ScanProsite:PS00331	PANTHER:PTHR23406	PANTHER:PTHR23406:SF39	SMART:SM00919
SMART:SM01274	SUPFAM:SSF51735	SUPFAM:SSF53223	TIGR:TC57980	EMBL:U67426	UniParc:UPI0001983F7A
ArrayExpress:VIT_09s0002g03620	EnsemblPlantsGene:VIT_09s0002g03620	EnsemblPlants:VIT_09s0002g03620.t01	EntrezGene:VVME2	wikigene:VVME2	unigene:Vvi.25305
unigene:Vvi.4987	SEG:seg	:	:	:	:

Description

Malic enzyme [Source:UniProtKB/TrEMBL;Acc:D7U0C2]

Coordinates

chr9:-:3297048..3303772

Molecular Weight (calculated)

70018.3 Da

IEP (calculated)

6.338

GRAVY (calculated)

-0.084

Length

640 amino acids

Sequence
(BLAST)

001: MISLNSSSFL SNSGFSGISR SVSQRKVLPP VVLALGSNGR AGELNVSAVM ESTLKELRDG NTVLEVDSKS AVSGGVRDVQ GEDAATEDQI VTPWTITVAS
101: GYSLLRNPHH NKGLAFTEKE RDHHYLRGLL PPAVVSQDLQ VKKLMANIRQ YTVPLQKYMA MMDLQERNEK LFYKLLMDNV EELLPVVYTP TVGEACQKYG
201: GILRRPQGLF ISLNEKGKIL EVLKNWPEKN IQVIVVTDGE RILGLGDLGC QGMGIPVGKL SLYTALGGVR PSACLPVTID VGTNNQKLLD DEFYIGLRQK
301: RATGQEYAEL IHEFMCAVKQ NYGEKVLVQF EDFANHNAFD LLARYGTTHL VFNDDIQGTA SVVLAGLISA LNLVGGTLAE HTFLFLGAGE AGTGIAELIA
401: LEMSKQTQAP LEETRKKIWL VDSKGLIVSS RMDSLQHFKK PWAHEHEPIK NLVDAVKAIK PTVLIGSSGV GRTFTKEVVE AMASFNEKPI ILALSNPTSQ
501: SECTAEEAYT WSEGRAIFAS GSPFDPVEYN GKVFVPGQSN NAYIFPGFGL GLIISGAIRV HDDMLLAASE ALAKQATQEN FDKGMIYPPF SNIRKISAHI
601: AANVAAKAYE LGLATRLPQP ENLVEYAENC MYSPVYRSFR

Best Arabidopsis Sequence Match ( AT1G79750.1 )
(BLAST)

001: MISLTPSLFL NKTVVPGCST RLSLRQPRTI ITPPASLRVF SSLGSNQDPT GSVLIETTAT SSSSLETSAA DIVPKSTVSG GVQDVYGEDA ATEDMPITPW
101: SLSVASGYTL LRDPHHNKGL AFSHRERDAH YLRGLLPPTV ISQDLQVKKI MHTLRQYQVP LQKYMAMMDL QETNERLFYK LLIDHVEELL PVIYTPTVGE
201: ACQKYGSIFL RPQGLFISLK EKGKIHEVLR NWPEKNIQVI VVTDGERILG LGDLGCQGMG IPVGKLSLYT ALGGVRPSAC LPVTIDVGTN NEKLLNDEFY
301: IGLRQRRATG EEYSELMHEF MTAVKQNYGE KVVIQFEDFA NHNAFDLLAK YGTTHLVFND DIQGTASVVL AGLIAALRFV GGSLSDHRFL FLGAGEAGTG
401: IAELIALEIS KKSHIPLEEA RKNIWLVDSK GLIVSSRKES IQHFKKPWAH DHEPIRELVD AVKAIKPTVL IGTSGVGQTF TQDVVETMAK LNEKPIILSL
501: SNPTSQSECT AEEAYTWSQG RAIFASGSPF APVEYEGKTF VPGQANNAYI FPGFGLGLIM SGTIRVHDDM LLAASEALAE ELMEEHYEKG MIYPPFRNIR
601: KISARIAAKV AAKAYELGLA TRLPQPKELE QCAESSMYSP SYRSYR

Arabidopsis Description

NADP-ME4Malic enzyme [Source:UniProtKB/TrEMBL;Acc:A0A178W4H6]
SUBAcon: [plastid]

Hydropathy Plot

About CropPAL

The Protein Annotated Locations Database (CropPAL) houses large scale proteomic and GFP localization data from published experimental studies in Soybean (Glycine max), Maize (Zea mays), Wheat (Triticum aestivum), Barley (Hordeum vulgare), Rice (Oryza sativa), Field mustard (Brassica rapa), Canola (Brassica napus), Sorghum (Sorghum bicolor), Potato (Solanum tuberosum), Tomato (Solanum lycopersicum), Banana (Musa acuminata) and Wine grape (Vitis vinifera) as well as precomputed predictions for protein subcellular localizations using protein sequences.