Factsheet for PGSC0003DMT400000066

Subcellular Localization

min:

: max

Winner_takes_all: mitochondrion

Predictor Summary:

plastid 1
mitochondrion 9

Predictors

GFP

MS/MS

Papers

mitochondrion: 14983062
cytosol: 22313117

msms PMID: 22313117 doi

S Lim, K Chisholm, RH Coffin, RD Peters, KI Al-Mughrabi, G Wang-Pruski, DM Pinto

Department of Plant and Animal Sciences, Nova Scotia Agricultural College, Truro, Nova Scotia, Canada.

msms PMID: 14983062 doi

Y Balmer, WH Vensel, CK Tanaka, WJ Hurkman, E Gelhaye, N Rouhier, JP Jacquot, W Manieri, P Schürmann, M Droux, BB Buchanan

Department of Plant and Microbial Biology, University of California, 111 Koshland Hall, Berkeley, CA 94720, USA.

PPI

Inferred distinct locusB in Crop

locusB	locations
PGSC0003DMT400040141
PGSC0003DMT400040142
PGSC0003DMT400040143
PGSC0003DMT400040144
PGSC0003DMT400040206
PGSC0003DMT400040207
PGSC0003DMT400077934

Inferred from Arabidopsis experimental PPI

Ath locusA	locusB	Ath locusB	Paper
AT4G00570.1	PGSC0003DMT400040141	AT3G46090.1	17259181
AT4G00570.1	PGSC0003DMT400040142	AT3G46090.1	17259181
AT4G00570.1	PGSC0003DMT400040143	AT3G46090.1	17259181
AT4G00570.1	PGSC0003DMT400040144	AT3G46090.1	17259181
AT4G00570.1	PGSC0003DMT400040206	AT3G46090.1	17259181
AT4G00570.1	PGSC0003DMT400040207	AT3G46090.1	17259181
AT4G00570.1	PGSC0003DMT400077934	AT3G46090.1	17259181

Homology

Paralog

locus	Identity	Homology Identity

Ortholog

locus	Homology Species	Location	Identity	Homology Identity
Solyc01g094200.2.1	Tomato	plastid	95.37	99.5
VIT_15s0046g03670.t01	Wine grape	mitochondrion	81.02	83.97
KRH48207	Soybean	mitochondrion	78.95	81.95
KRH65116	Soybean	mitochondrion	78.79	81.79
KRH00689	Soybean	mitochondrion	77.67	81.17
KRH40505	Soybean	mitochondrion	77.03	80.37
Bra037347.1-P	Field mustard	mitochondrion	74.96	80.07
CDY68746	Canola	mitochondrion	77.03	79.83
CDY51151	Canola	mitochondrion	77.03	79.57
CDX74340	Canola	mitochondrion	77.03	79.44
CDY07209	Canola	mitochondrion	77.03	79.31
GSMUA_Achr1P00210_001	Banana	mitochondrion	76.08	78.97
Bra000967.1-P	Field mustard	mitochondrion	77.03	77.9
AT4G00570.1	Thale cress	mitochondrion	74.64	77.1
KXG38219	Sorghum	mitochondrion	75.12	76.21
TraesCS1D01G164800.4	Wheat	mitochondrion, unclear	73.68	74.88
Os10t0503500-01	Rice	mitochondrion	73.68	74.52
TraesCS1B01G190400.5	Wheat	mitochondrion	71.61	73.13
Zm00001d013911_P001	Maize	mitochondrion	71.13	69.69
HORVU1Hr1G045720.1	Barley	plastid	73.68	63.55
PGSC0003DMT400077452	Potato	cytosol	37.48	39.76
PGSC0003DMT400000961	Potato	cytosol	37.16	39.56
PGSC0003DMT400084201	Potato	cytosol	36.52	39.55
PGSC0003DMT400068027	Potato	plastid	36.84	36.09
PGSC0003DMT400006726	Potato	plastid	36.52	35.67
PGSC0003DMT400043836	Potato	cytosol	12.28	32.08
PGSC0003DMT400008389	Potato	mitochondrion	13.24	31.32

Protein Annotations

MapMan:2.3.9	Gene3D:3.40.50.10380	GO:GO:0000166	GO:GO:0003674	GO:GO:0003824	GO:GO:0004470
GO:GO:0004471	GO:GO:0005488	GO:GO:0005575	GO:GO:0005622	GO:GO:0005623	GO:GO:0005737
GO:GO:0005739	GO:GO:0005759	GO:GO:0008150	GO:GO:0008152	GO:GO:0016491	GO:GO:0046872
GO:GO:0051287	GO:GO:0055114	InterPro:IPR037062	UniProt:M0ZG23	InterPro:Malic_NAD-bd	InterPro:Malic_N_dom
InterPro:Malic_N_dom_sf	InterPro:Malic_OxRdtase	InterPro:Malic_enzyme_CS	InterPro:NAD(P)-bd_dom_sf	PFAM:PF00390	PFAM:PF03949
EnsemblPlantsGene:PGSC0003DMG401000020	PGSC:PGSC0003DMG401000020	EnsemblPlants:PGSC0003DMT400000066	PIRSF:PIRSF000106	PRINTS:PR00072	ScanProsite:PS00331
PANTHER:PTHR23406	PANTHER:PTHR23406:SF32	SMART:SM00919	SMART:SM01274	SUPFAM:SSF51735	SUPFAM:SSF53223
UniParc:UPI000294EC71	SEG:seg	:	:	:	:

Description

NAD-dependent malic enzyme 59 kDa isoform, mitochondrial [Source:PGSC_GENE;Acc:PGSC0003DMG401000020]

Coordinates

chr1:+:71807804..71817295

Molecular Weight (calculated)

69178.3 Da

IEP (calculated)

6.356

GRAVY (calculated)

-0.115

Length

627 amino acids

Sequence
(BLAST)

001: MWRVARSAAS TFRRTRRLST AISAPCIVHK RGADILHDPW FNKDTGFPMT ERDRLGLRGL LPPRVISFEQ QYDRFMESFR SLEKNTEGQP DSVVSLAKWR
101: ILNRLHDRNE TLYYRVLIDN IKDFAPIIYT PTVGLVCQNY SGLFRRPRGM YFSAKDKGEM MSMIFNWPST QVDMIVLTDG SRILGLGDLG VQGIGIPIGK
201: LDMYVAAAGI NPQRVLPVML DVGTNNQKLL EDPLYLGLRQ PRLEGEEYLS IVDEFVEAVH ARWPKAVVQF EDFQAKWAFE TLDRYRKKFC MFNDDIQGTA
301: GVALAGLLGT VRAQGRPLTD FANQKIVVVG AGSAGLGVLK MALQAVSRMT GPSADPHFFL LDKNGLITKD RKDIDPAALP FAKAHHEIEG LGLQEGAGLA
401: EVGTNNCYLH LSAFYAFQDN KAGLNVCQVK KVKPHVLLGL SGVGGIFHEE VLRAMKESDS VRPAIFAMSN PTNNAECCPV DAFKLAGEDI VFASGSPFAN
501: VDLGNGKIGH VNQANNMYLF PGIGLGALLS GARNISDTML EAAAECLASY MSDDEINRGI LYPSIDDIRD ITAEVGAAVL RAAVAEDLAE GHGDVGVREL
601: QHMSKEETIE HVRQNMWYPV YGPLVHE

Best Arabidopsis Sequence Match ( AT4G00570.1 )
(BLAST)

001: MMWKNIAGLS KAAAAARTHG SRRCFSTAIP GPCIVHKRGA DILHDPWFNK DTGFPLTERD RLGIRGLLPP RVMTCVQQCD RFIESFRSLE NNTKGEPENV
101: VALAKWRMLN RLHDRNETLY YRVLIDNIKD FAPIIYTPTV GLVCQNYSGL YRRPRGMYFS AKDKGEMMSM IYNWPAPQVD MIVITDGSRI LGLGDLGVQG
201: IGIPIGKLDM YVAAAGINPQ RVLPIMLDVG TNNEKLLQND LYLGVRQPRL EGEEYLEIID EFMEAAFTRW PKAVVQFEDF QAKWAFGTLE RYRKKFCMFN
301: DDVQGTAGVA LAGLLGTVRA QGRPISDFVN QKIVVVGAGS AGLGVTKMAV QAVARMAGIS ESEATKNFYL IDKDGLVTTE RTKLDPGAVL FAKNPAEIRE
401: GASIVEVVKK VRPHVLLGLS GVGGIFNEEV LKAMRESDSC KPAIFAMSNP TLNAECTAAD AFKHAGGNIV FASGSPFENV ELENGKVGHV NQANNMYLFP
501: GIGLGTLLSG ARIVTDGMLQ AASECLASYM TDEEVQKGIL YPSINNIRHI TAEVGAAVLR AAVTDDIAEG HGDVGPKDLS HMSKEDTVNY ITRNMWFPVY
601: SPLVHEK

Arabidopsis Description

NAD-ME2NAD-dependent malic enzyme 2, mitochondrial [Source:UniProtKB/Swiss-Prot;Acc:Q8L7K9]
SUBAcon: [mitochondrion]

Hydropathy Plot

About CropPAL

The Protein Annotated Locations Database (CropPAL) houses large scale proteomic and GFP localization data from published experimental studies in Soybean (Glycine max), Maize (Zea mays), Wheat (Triticum aestivum), Barley (Hordeum vulgare), Rice (Oryza sativa), Field mustard (Brassica rapa), Canola (Brassica napus), Sorghum (Sorghum bicolor), Potato (Solanum tuberosum), Tomato (Solanum lycopersicum), Banana (Musa acuminata) and Wine grape (Vitis vinifera) as well as precomputed predictions for protein subcellular localizations using protein sequences.