Factsheet for Os04t0480200-01

Subcellular Localization

min:

: max

Winner_takes_all: plasma membrane

Predictor Summary:

extracellular 5
endoplasmic reticulum 6
vacuole 5
plasma membrane 7
golgi 6
plastid 2
nucleus 1

Predictors

GFP

MS/MS

Papers

Winner Takes All:	plasma membrane
Any Predictor:	nucleus, plastid, secretory
BaCelLo:	secretory
iPSORT:	secretory
MultiLoc:	endoplasmic reticulum
Plant-mPloc:	golgi, nucleus, plasma membrane, plastid
Predotar:	secretory
PProwler:	secretory
TargetP:	secretory
WoLF PSORT:	plastid
YLoc:	plasma membrane

plasma membrane: 18260611

msms PMID: 18260611 doi

SH Natera, KL Ford, AM Cassin, JH Patterson, EJ Newbigin, A Bacic

Plant Cell Biology Research Centre, VIC, Australia. s.natera@unimelb.edu.au

PPI

Inferred distinct locusB in Crop

locusB	locations
Os02t0112100-01
Os02t0112600-01

Inferred from Arabidopsis experimental PPI

Ath locusA	locusB	Ath locusB	Paper
AT5G50200.1	Os02t0112100-01	AT1G08090.1	17012411
AT5G50200.1	Os02t0112600-01	AT1G08090.1	17012411
AT5G50200.1	Os02t0112100-01	AT5G60780.1	17012411
AT5G50200.1	Os02t0112600-01	AT5G60780.1	17012411

Homology

Paralog

locus	Identity	Homology Identity

Ortholog

locus	Homology Species	Location	Identity	Homology Identity
KXG26563	Sorghum	extracellular, plasma membrane, vacuole	67.62	65.44
Zm00001d003287_P001	Maize	endoplasmic reticulum, plasma membrane, vacuole	57.14	60.91
Os02t0595900-01	Rice	plasma membrane	57.62	58.74
GSMUA_Achr6P12130_001	Banana	endoplasmic reticulum, extracellular	50.95	53.5
GSMUA_Achr9P01940_001	Banana	endoplasmic reticulum	47.62	50.51
GSMUA_Achr10P...	Banana	endoplasmic reticulum, plasma membrane	47.62	49.02
VIT_17s0000g09470.t01	Wine grape	endoplasmic reticulum, vacuole	44.76	46.77
KRH57152	Soybean	endoplasmic reticulum, extracellular	40.48	40.87
KRH03862	Soybean	endoplasmic reticulum, extracellular	40.48	40.87
Solyc03g112100.2.1	Tomato	endoplasmic reticulum, extracellular, plasma membrane	38.57	39.9
Bra013828.1-P	Field mustard	cytosol	29.05	39.61
Bra000613.1-P	Field mustard	endoplasmic reticulum, extracellular, plasma membrane	38.57	39.32
CDY64637	Canola	endoplasmic reticulum, extracellular, plasma membrane	38.57	39.32
CDY34047	Canola	endoplasmic reticulum, extracellular, plasma membrane	38.1	38.84
AT5G50200.1	Thale cress	endoplasmic reticulum, extracellular, plasma membrane	38.57	38.57
CDX71613	Canola	endoplasmic reticulum, extracellular	38.57	38.21
Bra037934.1-P	Field mustard	endoplasmic reticulum, extracellular	37.62	37.98
CDX83019	Canola	endoplasmic reticulum, extracellular, plasma membrane	37.14	37.86
Bra033790.1-P	Field mustard	endoplasmic reticulum, extracellular, plasma membrane	37.14	37.86
CDX91545	Canola	endoplasmic reticulum, extracellular, plasma membrane	37.62	37.26
CDY16372	Canola	extracellular	37.14	36.79
Bra022511.1-P	Field mustard	extracellular	37.14	36.79
CDY05799	Canola	cytosol, endoplasmic reticulum, plastid	19.52	36.28
CDY03424	Canola	endoplasmic reticulum, extracellular, plasma membrane	35.24	35.58
AT4G24715.1	Thale cress	endoplasmic reticulum, extracellular, plasma membrane	31.9	29.0
CDY34305	Canola	extracellular, plasma membrane, plastid	35.71	28.63
Solyc03g112090.2.1	Tomato	extracellular	14.76	26.96
PGSC0003DMT400046695	Potato	endoplasmic reticulum, golgi, plasma membrane	40.0	25.38

Protein Annotations

MapMan:25.1.3.2	EntrezGene:4336175	EMBL:AK109571	ProteinID:BAF15013.1	ProteinID:BAS89729.1	ProteinID:CAE05891.1
GO:GO:0005575	GO:GO:0005623	GO:GO:0005886	GO:GO:0006810	GO:GO:0008150	GO:GO:0008152
GO:GO:0009987	GO:GO:0010167	GO:GO:0015706	GO:GO:0016020	GO:GO:0016021	GO:GO:0042128
EnsemblPlantsGene:Os04g0480200	EnsemblPlants:Os04t0480200-01	PFAM:PF16974	PIRSF:PIRSF012939	PFscan:PS51257	PANTHER:PTHR34806
PANTHER:PTHR34806:SF2	UniProt:Q7XK12	SignalP:SignalP-noTM	TMHMM:TMhelix	InterPro:Transptr_NO3_Nar2	UniParc:UPI00001B1CDC
RefSeq:XP_015634457.1	SEG:seg	:	:	:	:

Description

ACTIVATOR FOR NRT2Transporter, high affinity nitrate, Nar2 domain containing protein. (Os04t0480200-01)

Coordinates

chr4:-:24018298..24019456

Molecular Weight (calculated)

21537.0 Da

IEP (calculated)

9.713

GRAVY (calculated)

0.413

Length

210 amino acids

Sequence
(BLAST)

001: MARFGAVIHR VFLPLLLLLV VLGACHVTPA AAAAGARLSA LAKALVVEAS PRAGQVLHAG EDAITVTWSL NATAAAAAAG ADAGYKAVKV TLCYAPASQV
101: GRGWRKAHDD LSKDKACQFK IAQQPYDGAG KFEYTVARDV PTASYYVRAY ALDASGARVA YGETAPSASF AVAGITGVTA SIEVAAGVLS AFSVAALAVF
201: LVLENKKKNK

Best Arabidopsis Sequence Match ( AT5G50200.1 )
(BLAST)

001: MAIQKILFAS LLICSLIQSI HGAEKVRLFK ELDKGALDVT TKPSREGPGV VLDAGKDTLN ITWTLSSIGS KREAEFKIIK VKLCYAPPSQ VDRPWRKTHD
101: ELFKDKTCPH KIIAKPYDKT LQSTTWTLER DIPTGTYFVR AYAVDAIGHE VAYGQSTDDA KKTNLFSVQA ISGRHASLDI ASICFSVFSV VALVVFFVNE
201: KRKAKIEQSK

Arabidopsis Description

NRT3.1High-affinity nitrate transporter 3.1 [Source:UniProtKB/Swiss-Prot;Acc:Q9FGS5]
SUBAcon: [endoplasmic reticulum,extracellular,plasma membrane]

Hydropathy Plot

About CropPAL

The Protein Annotated Locations Database (CropPAL) houses large scale proteomic and GFP localization data from published experimental studies in Soybean (Glycine max), Maize (Zea mays), Wheat (Triticum aestivum), Barley (Hordeum vulgare), Rice (Oryza sativa), Field mustard (Brassica rapa), Canola (Brassica napus), Sorghum (Sorghum bicolor), Potato (Solanum tuberosum), Tomato (Solanum lycopersicum), Banana (Musa acuminata) and Wine grape (Vitis vinifera) as well as precomputed predictions for protein subcellular localizations using protein sequences.