Factsheet for Bra027457.1-P

Subcellular Localization

min:

: max

Winner_takes_all: cytosol

Predictor Summary:

cytosol 3
plastid 2
extracellular 1
golgi 2
plasma membrane 1
endoplasmic reticulum 1
vacuole 1
mitochondrion 1

Predictors

GFP

MS/MS

Papers

Winner Takes All:	cytosol
Any Predictor:	cytosol, mitochondrion, plastid, secretory
BaCelLo:	cytosol
EpiLoc:	plastid
iPSORT:	secretory
MultiLoc:	cytosol
Plant-mPloc:	golgi
PProwler:	mitochondrion
WoLF PSORT:	plastid
YLoc:	cytosol

PPI

Inferred distinct locusB in Crop

locusB	locations
Bra003209.1-P
Bra006224.1-P
Bra007142.1-P
Bra007145.1-P
Bra008792.1-P
Bra009358.1-P
Bra017728.1-P
Bra023441.1-P
Bra036307.1-P

Inferred from Arabidopsis experimental PPI

Ath locusA	locusB	Ath locusB	Paper
AT5G42800.1	Bra003209.1-P	AT3G55120.1	10536025
AT5G42800.1	Bra007142.1-P	AT3G55120.1	10536025
AT5G42800.1	Bra007145.1-P	AT3G55120.1	10536025
AT5G42800.1	Bra017728.1-P	AT3G55120.1	10536025
AT5G42800.1	Bra009358.1-P	AT5G08640.1	18467451
AT5G42800.1	Bra006224.1-P	AT5G13930.1	10536025
AT5G42800.1	Bra008792.1-P	AT5G13930.1	10536025
AT5G42800.1	Bra023441.1-P	AT5G13930.1	10536025
AT5G42800.1	Bra036307.1-P	AT5G13930.1	10536025
AT5G42800.1	Bra006224.1-P	AT5G13930.1	21669202
AT5G42800.1	Bra008792.1-P	AT5G13930.1	21669202
AT5G42800.1	Bra023441.1-P	AT5G13930.1	21669202
AT5G42800.1	Bra036307.1-P	AT5G13930.1	21669202

Homology

Paralog

locus	Identity	Homology Identity

Ortholog

locus	Homology Species	Location	Identity	Homology Identity
CDY52372	Canola	cytosol	100.0	100.0
AT5G42800.1	Thale cress	cytosol	86.23	86.91
VIT_04s0023g02090.t01	Wine grape	cytosol	28.05	67.08
KRH15169	Soybean	plastid	59.22	65.52
KRH05848	Soybean	plastid	59.74	64.97
EES01135	Sorghum	cytosol	56.1	61.71
KRH71611	Soybean	nucleus	54.29	61.65
PGSC0003DMT400009287	Potato	cytosol	60.0	60.47
Solyc02g085020.2.1	Tomato	cytosol	59.48	60.42
GSMUA_Achr4P24540_001	Banana	cytosol, golgi, plastid	54.81	59.94
TraesCS3D01G224600.1	Wheat	cytosol, extracellular, plastid	55.06	59.89
TraesCS3A01G226600.1	Wheat	nucleus	54.55	59.32
Zm00001d044122_P001	Maize	plastid	54.55	58.82
EES19023	Sorghum	cytosol, peroxisome, plastid	55.06	58.56
HORVU3Hr1G056560.2	Barley	cytosol	53.77	58.47
EES04562	Sorghum	plastid	54.55	58.01
Zm00001d011438_P001	Maize	extracellular	53.77	57.34
Os01t0633500-00	Rice	cytosol, mitochondrion, plasma membrane, plastid	45.71	55.52
TraesCS3B01G257900.2	Wheat	plastid	55.58	55.44
EES01136	Sorghum	plastid	54.81	54.24
HORVU6Hr1G047380.1	Barley	peroxisome	18.7	45.57
KRH05851	Soybean	extracellular	14.29	44.35
HORVU6Hr1G048650.1	Barley	endoplasmic reticulum, plasma membrane	17.66	42.77
Bra021318.1-P	Field mustard	cytosol	35.58	39.71
Bra031403.1-P	Field mustard	cytosol	34.29	39.05
Bra004907.1-P	Field mustard	cytosol	31.43	38.78
Bra040313.1-P	Field mustard	cytosol, golgi, plastid	34.55	37.15
Bra019062.1-P	Field mustard	cytosol	33.25	36.16
Bra013768.1-P	Field mustard	cytosol	17.92	30.94
Bra013769.1-P	Field mustard	nucleus	20.52	26.07
Bra030206.1-P	Field mustard	cytosol	20.78	23.81

Protein Annotations

Gene3D:3.40.50.720	MapMan:9.2.2.9.1	ProteinID:AAV80210	ProteinID:AAV80210.1	EMBL:AY567978	EnsemblPlantsGene:Bra027457
EnsemblPlants:Bra027457.1	EnsemblPlants:Bra027457.1-P	InterPro:Epimerase_deHydtase	GO:GO:0003674	GO:GO:0003824	GO:GO:0005488
GO:GO:0050662	UniProt:M4EF95	InterPro:NAD(P)-bd_dom_sf	PFAM:PF01370	PANTHER:PTHR10366	PANTHER:PTHR10366:SF276
SUPFAM:SSF51735	UniParc:UPI0000EBF6B5	:	:	:	:

Description

AT5G42800 (E=3e-194) DFR, TT3, M318 | DFR (DIHYDROFLAVONOL 4-REDUCTASE); dihydrokaempferol 4-reductase

Coordinates

chrA09:+:14186276..14187832

Molecular Weight (calculated)

42888.4 Da

IEP (calculated)

5.634

GRAVY (calculated)

-0.255

Length

385 amino acids

Sequence
(BLAST)

001: MVAHKETVCV TGASGFIGSW LVMRLLERGY FVRATVRDPG NLKKVQHLLD LPNAKTQLTL WKADLSDEGS YDDAINGCDG VFHIATPMDF ESKDPENEVI
101: KPTVNGVLGI MKACDKAKTV RRIVFTSSAG TVNVEEHQKN VYDENDWSDL DFIMSKKMTG WMYFMSKTLA EKAAWDYAKE KGIDFISIIP TLVIGPFITT
201: SMPPSLITAL SPITRNEAHY SIIRQGQYVH LDDLCNAHIF LYEQAAAKGR YVCSSHDATI LTISEFLRQK YPEYNVPSTF EGVDENLKSI MFSSKKLIDM
301: GFNFKYSLED MLVESIETCR QKGFLPVTLP EHLKSEDKVP GSDDNKEIKN GSAGLTDGMV ACKKTEPGMA GEKADSHMSA QQICA

Best Arabidopsis Sequence Match ( AT5G42800.1 )
(BLAST)

001: MVSQKETVCV TGASGFIGSW LVMRLLERGY FVRATVRDPG NLKKVQHLLD LPNAKTLLTL WKADLSEEGS YDDAINGCDG VFHVATPMDF ESKDPENEVI
101: KPTVNGMLGI MKACVKAKTV RRFVFTSSAG TVNVEEHQKN VYDENDWSDL EFIMSKKMTG WMYFVSKTLA EKAAWDFAEE KGLDFISIIP TLVVGPFITT
201: SMPPSLITAL SPITRNEAHY SIIRQGQYVH LDDLCNAHIF LYEQAAAKGR YICSSHDATI LTISKFLRPK YPEYNVPSTF EGVDENLKSI EFSSKKLTDM
301: GFNFKYSLEE MFIESIETCR QKGFLPVSLS YQSISEIKTK NENIDVKTGD GLTDGMKPCN KTETGITGER TDAPMLAQQM CA

Arabidopsis Description

DFRADihydroflavonol reductase [Source:UniProtKB/TrEMBL;Acc:B1GV15]
SUBAcon: [cytosol]

Hydropathy Plot

About CropPAL

The Protein Annotated Locations Database (CropPAL) houses large scale proteomic and GFP localization data from published experimental studies in Soybean (Glycine max), Maize (Zea mays), Wheat (Triticum aestivum), Barley (Hordeum vulgare), Rice (Oryza sativa), Field mustard (Brassica rapa), Canola (Brassica napus), Sorghum (Sorghum bicolor), Potato (Solanum tuberosum), Tomato (Solanum lycopersicum), Banana (Musa acuminata) and Wine grape (Vitis vinifera) as well as precomputed predictions for protein subcellular localizations using protein sequences.