CropPAL

Subcellular Localization

min:

: max

Winner_takes_all: nucleus, cytosol

Predictor Summary:

cytosol 4
nucleus 1
plastid 1
mitochondrion 1

Predictors

GFP

MS/MS

Papers

nucleus: 21132161

msms PMID: 21132161 doi

B Cooper, KB Campbell, J Feng, WM Garrett, R Frederick

Soybean Genomics and Improvement Laboratory, USDA-ARS, Beltsville, MD 20705, USA. bret.cooper@ars.usda.gov

PPI

Inferred distinct locusB in Crop

locusB	locations
KRG91803
KRH02720
KRH19149
KRH34983
KRH34984
KRH52586
KRH61926

Inferred from Arabidopsis experimental PPI

Ath locusA	locusB	Ath locusB	Paper
AT1G65650.1	KRG91803	AT1G35670.1	19130088
AT1G65650.1	KRH34983	AT1G35670.1	19130088
AT1G65650.1	KRH34984	AT1G35670.1	19130088
AT5G16310.1	KRH52586	AT1G71230.1	21798944
AT5G16310.1	KRH61926	AT1G71230.1	21798944
AT5G16310.1	KRH02720	AT2G19560.1	19843313
AT5G16310.1	KRH19149	AT2G19560.1	19843313

Homology

Paralog

locus	Identity	Homology Identity

Ortholog

locus	Homology Species	Location	Identity	Homology Identity
KRH34213	Soybean	cytosol	98.2	98.2
VIT_04s0044g02040.t01	Wine grape	cytosol	90.12	90.12
PGSC0003DMT400052269	Potato	cytosol	89.52	89.52
GSMUA_Achr3P00030_001	Banana	cytosol	83.23	89.39
Solyc09g018730.2.1	Tomato	cytosol, nucleus	83.83	88.89
GSMUA_AchrUn_...	Banana	cytosol	62.57	80.08
AT1G65650.1	Thale cress	cytosol	79.04	80.0
CDY39276	Canola	cytosol	78.14	79.33
Bra004123.1-P	Field mustard	cytosol	77.84	79.03
CDY07458	Canola	cytosol	77.84	79.03
AT5G16310.1	Thale cress	cytosol	70.36	70.36
Os02t0180100-01	Rice	nucleus	68.86	69.91
CDX70685	Canola	cytosol	68.86	69.91
CDX78681	Canola	cytosol	70.06	69.44
EES14068	Sorghum	cytosol	67.37	67.98
TraesCS6D01G137100.1	Wheat	cytosol	66.47	67.68
TraesCS6A01G147800.1	Wheat	cytosol	66.17	67.38
TraesCS6B01G176100.1	Wheat	cytosol	66.17	67.38
EER95226	Sorghum	cytosol	66.47	67.07
HORVU6Hr1G029150.7	Barley	cytosol	64.07	65.05
Zm00001d049911_P001	Maize	cytosol	59.58	63.78
Zm00001d031894_P005	Maize	cytosol	65.57	61.69
HORVU6Hr1G083540.21	Barley	plastid	41.32	44.09
HORVU5Hr1G105430.16	Barley	endoplasmic reticulum, peroxisome, plasma membrane	32.04	33.97

Protein Annotations

Gene3D:1.20.58.860	EntrezGene:100792356	MapMan:19.2.2.1.2.1	MapMan:19.2.2.3.2.1	MapMan:19.2.6.2.3	Gene3D:3.40.532.10
EMBL:ACUP02012763	MEROPS:C12.A01	ncoils:Coil	EnsemblPlantsGene:GLYMA_20G219500	GO:GO:0003674	GO:GO:0003824
GO:GO:0004843	GO:GO:0005575	GO:GO:0005622	GO:GO:0005623	GO:GO:0005737	GO:GO:0006464
GO:GO:0006508	GO:GO:0006511	GO:GO:0008150	GO:GO:0008152	GO:GO:0008233	GO:GO:0008234
GO:GO:0009056	GO:GO:0009987	GO:GO:0016579	GO:GO:0016787	GO:GO:0019538	GO:GO:0036459
UniProt:I1NIJ5	InterPro:IPR036959	EnsemblPlants:KRG92573	ProteinID:KRG92573	ProteinID:KRG92573.1	ProteinID:KRG92574.1
PFAM:PF01088	PIRSF:PIRSF038120	PRINTS:PR00707	PANTHER:PTHR10589	PANTHER:PTHR10589:SF30	InterPro:Papain_like_cys_pep_sf
InterPro:Peptidase_C12_UCH	InterPro:Peptidase_C12_UCH_sf	SUPFAM:SSF54001	UniParc:UPI000233DCB5	InterPro:Ubiquitinyl_hydrolase_UCH37	SEG:seg

Description

hypothetical protein

Coordinates

chr20:+:45505965..45509832

Molecular Weight (calculated)

38328.1 Da

IEP (calculated)

6.335

GRAVY (calculated)

-0.442

Length

334 amino acids

Sequence
(BLAST)

001: MSWCTIESDP GVFTELIQQM QVKGVQVEEL YSLDLDSLNS LRPVYGLIFL FKWRPGEKDD RVVIKDPNPN LFFASQVINN ACATQAILSI LMNSPDIDIG
101: PELTKLKEFT KNFPPELKGL AINNSEAIRT AHNSFARPEP FVPEEQKVAS KDDDVYHFIS YLPVDGVLYE LDGLKEGPIS LGQCSGGQGD MEWLKMVQPV
201: IQERIERYSQ SEIRFNLLAV IKNRKEMYTA ELKELQKRRE RILQQLAASK SDRLVDNSSF EALNNSLSEV NAGIEAATEK ILMEEEKFKK WRTENIRRKH
301: NYIPFLFNFL KILAEKKQLK PLIEKAKQKT SSPR

Best Arabidopsis Sequence Match ( AT1G65650.1 )
(BLAST)

001: MSWCTIESDP GVFTELIQQM QVKGVQVEEL YSLDSDSLNN LRPVYGLIFL FKWQAGEKDE RPTIQDQVSN LFFANQVINN ACATQAILAI LLNSPEVDIG
101: PELSALKEFT KNFPSDLKGL AINNSDSIRA AHNSFARPEP FVPEEQKAAT KDDDVYHFIS YIPVDGVLYE LDGLKEGPIS LGPCPGDQTG IEWLQMVQPV
201: IQERIERYSQ SEIRFNLLAV IKNRKDIYTA ELKELQRQRE QLLQQANTCV DKSEAEAVNA LIAEVGSGIE AASDKIVMEE EKFMKWRTEN IRRKHNYIPF
301: LFNFLKLLAE KKQLKPLIEK AKKQKTESST

Arabidopsis Description

UCH2Ubiquitin carboxyl-terminal hydrolase [Source:UniProtKB/TrEMBL;Acc:A0A178WM46]
SUBAcon: [cytosol]

Hydropathy Plot

About CropPAL

The Protein Annotated Locations Database (CropPAL) houses large scale proteomic and GFP localization data from published experimental studies in Soybean (Glycine max), Maize (Zea mays), Wheat (Triticum aestivum), Barley (Hordeum vulgare), Rice (Oryza sativa), Field mustard (Brassica rapa), Canola (Brassica napus), Sorghum (Sorghum bicolor), Potato (Solanum tuberosum), Tomato (Solanum lycopersicum), Banana (Musa acuminata) and Wine grape (Vitis vinifera) as well as precomputed predictions for protein subcellular localizations using protein sequences.