CropPAL

Subcellular Localization

min:

: max

Winner_takes_all: nucleus, cytosol

Predictor Summary:

plastid 2
cytosol 3
nucleus 1
mitochondrion 1

Predictors

GFP

MS/MS

Papers

nucleus: 21132161

msms PMID: 21132161 doi

B Cooper, KB Campbell, J Feng, WM Garrett, R Frederick

Soybean Genomics and Improvement Laboratory, USDA-ARS, Beltsville, MD 20705, USA. bret.cooper@ars.usda.gov

PPI

Inferred distinct locusB in Crop

locusB	locations
KRG92573
KRG92986
KRH04963
KRH11322
KRH16129
KRH20952
KRH34213
KRH36229
KRH54417
KRH54615
KRH56868
KRH56869
KRH56870
KRH63273
KRH63506

Inferred from Arabidopsis experimental PPI

Ath locusA	locusB	Ath locusB	Paper
AT2G19560.1	KRG92986	AT2G39340.1	19843313
AT2G19560.1	KRH36229	AT2G39340.1	19843313
AT2G19560.1	KRH11322	AT3G06290.1	19843313
AT2G19560.1	KRH20952	AT3G06290.1	19843313
AT2G19560.1	KRG92573	AT5G16310.1	19843313
AT2G19560.1	KRH34213	AT5G16310.1	19843313
AT2G19560.1	KRH04963	AT5G45010.1	19843313
AT2G19560.1	KRH16129	AT5G45010.1	19843313
AT2G19560.1	KRH54417	AT5G45010.1	19843313
AT2G19560.1	KRH63506	AT5G45010.1	19843313
AT2G19560.1	KRH54615	AT5G61290.1	19843313
AT2G19560.1	KRH56868	AT5G61290.1	19843313
AT2G19560.1	KRH56869	AT5G61290.1	19843313
AT2G19560.1	KRH56870	AT5G61290.1	19843313
AT2G19560.1	KRH63273	AT5G61290.1	19843313

Homology

Paralog

locus	Identity	Homology Identity

Ortholog

locus	Homology Species	Location	Identity	Homology Identity
KRH02720	Soybean	cytosol, nucleus	97.32	96.61
VIT_11s0016g02290.t01	Wine grape	cytosol	85.12	84.5
CDY67021	Canola	cytosol	71.46	81.62
CDY66989	Canola	cytosol	71.22	81.11
PGSC0003DMT400034206	Potato	cytosol	80.49	79.52
Bra040682.1-P	Field mustard	cytosol	80.0	79.42
CDY35873	Canola	cytosol	80.0	79.42
Solyc07g039210.2.1	Tomato	nucleus	80.0	79.04
AT2G19560.1	Thale cress	cytosol	79.02	78.45
PGSC0003DMT400056705	Potato	cytosol	33.9	78.09
GSMUA_Achr7P18480_001	Banana	cytosol	74.39	73.85
CDY13554	Canola	cytosol	30.24	71.68
Os09t0386400-01	Rice	cytosol	69.51	69.68
EER98853	Sorghum	cytosol	69.02	69.19
TraesCS5D01G207800.1	Wheat	cytosol	68.29	68.46
TraesCS5A01G201500.1	Wheat	cytosol	68.05	68.22
HORVU5Hr1G059930.4	Barley	cytosol	67.8	67.97
TraesCS5B01G200200.1	Wheat	cytosol	67.8	67.97
Zm00001d020359_P003	Maize	cytosol	69.27	66.36
CDY13555	Canola	cytosol, nucleus, vacuole	18.54	39.58

Protein Annotations

Gene3D:1.10.10.10	Gene3D:1.25.40.570	EntrezGene:100781018	MapMan:16.11.2.2	EMBL:ACUP02008170	EnsemblPlantsGene:GLYMA_13G103500
GO:GO:0000973	GO:GO:0003674	GO:GO:0003676	GO:GO:0003677	GO:GO:0003690	GO:GO:0003723
GO:GO:0005488	GO:GO:0005575	GO:GO:0005622	GO:GO:0005623	GO:GO:0005634	GO:GO:0005635
GO:GO:0005643	GO:GO:0006139	GO:GO:0006368	GO:GO:0006810	GO:GO:0008150	GO:GO:0008152
GO:GO:0009056	GO:GO:0009058	GO:GO:0009987	GO:GO:0016043	GO:GO:0016973	GO:GO:0035327
GO:GO:0070390	GO:GO:0071033	UniProt:I1LVJ3	InterPro:IPR000717	InterPro:IPR036388	EnsemblPlants:KRH19149
ProteinID:KRH19149	ProteinID:KRH19149.1	InterPro:PCI_dom	PFAM:PF01399	PFscan:PS50250	PANTHER:PTHR12732
PANTHER:PTHR12732:SF0	UniParc:UPI000233B93E	InterPro:WH-like_DNA-bd_sf	:	:	:

Description

hypothetical protein

Coordinates

chr13:+:21834734..21839761

Molecular Weight (calculated)

47169.6 Da

IEP (calculated)

9.812

GRAVY (calculated)

-0.153

Length

410 amino acids

Sequence
(BLAST)

001: MSMGEAHRRI TEYLNRFSDA ISSQDGATFK SLFALSSNSP FLLSLGDALN LFQDPNRLIK QSDNYSQFAD ILVPLFRSLL NYRQNNLLEA YNAFEKTANA
101: FIQEFRNWES AWALEALYVI VYDIRVLAEK ADKELASNGK SPEKLKGAGS VLMKFFGTLA GKGSKRVGAL YVTCQLFKIY FKLGTVHLCR SVIRSIETAR
201: IFDFEEFPKR DKVTYMYYTG RLEVFNENFP AADYKLSYAL KHCNPQSEAN IRMILKHLIP VKLSIGILPK NSLLEKYNLL EYSKIVQALR RGDLRLLRCA
301: LQDHEDRFLR SGVYLVLEKL ELQVYQRLVK KIYIIQKQKD PGRAHQVKLE VIVKALKWLE IDMDVDEVEC IVAILIYKNL MKGYFAHKSK VVVLSKQDPF
401: PKLNGKPVNS

Best Arabidopsis Sequence Match ( AT2G19560.1 )
(BLAST)

001: MAYVSMGEAH RRITEYLNRF CDAVSYQDSS TLCRLLSFSS NSPPLLSLAD ALNVFQDSSS LIRQSDRFSE YGEILAHVFR SLQSYRVGNL VEAYLAFDKF
101: ANAFVQEFRN WESAWALEAL YVVCYEIRVL AEKADKDLTS NGKSPEKLKA AGSLLMKVFG VLAGKGPKRV GALYVTCQLF KTYFKLGTVN LCRSVIRSIE
201: TARIFDFEEF PRRDKVTYMY YTGRLEVFNE NFPAADTKLS YALQNCNPKR ERNIRMILKY LVPVKLSLGI IPKDELLRNY NLHEYTKIVQ ALRKGDLRLL
301: RHALQEHEDR FLRSGVYLVL EKLELQVYQR LMKKIYINQK LSDPARAHQL KLEGIAKALR WLDMDMDLDE VECIMTILIY KNLVKGYLAH KSKVVVLSKQ
401: DPFPKLNGKP VSS

Arabidopsis Description

EER5Enhanced ethylene response protein 5 [Source:UniProtKB/Swiss-Prot;Acc:Q8GWE6]
SUBAcon: [cytosol]

Hydropathy Plot

About CropPAL

The Protein Annotated Locations Database (CropPAL) houses large scale proteomic and GFP localization data from published experimental studies in Soybean (Glycine max), Maize (Zea mays), Wheat (Triticum aestivum), Barley (Hordeum vulgare), Rice (Oryza sativa), Field mustard (Brassica rapa), Canola (Brassica napus), Sorghum (Sorghum bicolor), Potato (Solanum tuberosum), Tomato (Solanum lycopersicum), Banana (Musa acuminata) and Wine grape (Vitis vinifera) as well as precomputed predictions for protein subcellular localizations using protein sequences.